Abstract:
:We demonstrated that unmodified and modified (phosphorothioate) oligonucleotides prevent cDNA synthesis by AMV or HIV reverse transcriptases. Antisense oligonucleotide/RNA hybrids specifically arrest primer extension. The blockage involves the degradation of the RNA fragment bound to the antisense oligonucleotide by the reverse transcriptase-associated RNase H activity. However, the phosphorothioate oligomer inhibited polymerization by binding to the AMV RT rather than to the template RNA, whereas there was no competitive binding of the phosphorothioate oligomer on the HIV RT during reverse transcription.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Hatta T,Kim SG,Nakashima H,Yamamoto N,Sakamoto K,Yokoyama S,Takaku Hdoi
10.1016/0014-5793(93)80264-usubject
Has Abstractpub_date
1993-09-13 00:00:00pages
161-4issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(93)80264-Ujournal_volume
330pub_type
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