Abstract:
:Covalent, mechanism-based inhibitors of glycosidases are valuable probe molecules for visualizing enzyme activities in complex systems. We, here, describe the chemoenzymatic synthesis of 6-phospho-cyclophellitol and evaluate its behaviour as a mechanism-based inactivator of the Streptococcus pyogenes 6-phospho-β-glucosidase from CAZy family GH1. We further present the three-dimensional structure of the inactivated enzyme, which reveals the constellation of active site residues responsible for the enzyme's specificity and confirms the covalent nature of the inactivation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kwan DH,Jin Y,Jiang J,Chen HM,Kötzler MP,Overkleeft HS,Davies GJ,Withers SGdoi
10.1002/1873-3468.12059subject
Has Abstractpub_date
2016-02-01 00:00:00pages
461-8issue
4eissn
0014-5793issn
1873-3468journal_volume
590pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::JAK2 V617F mutant induces transformation through aberrant activation of various transcription factors. We found that the expression of Aurora kinase A (Aurka) was significantly induced by mutant JAK2 through c-Myc expression. Interestingly, mutant JAK2 enhanced resistance to cisplatin (CDDP)-induced DNA damage, and ef...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.04.068
更新日期:2011-06-23 00:00:00
abstract::Recent studies have implicated that a GTP-binding protein (G-protein) is involved in the coupling of both CCK-8 and muscarinic cholinergic receptors to phosphoinositidase C (PIC) in the human embryonic pituitary cell line, Flow 9000. Pretreatment of these cells with cholera toxin, but not pertussis toxin, inhibited th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80840-2
更新日期:1987-08-17 00:00:00
abstract::The fluorescence dye 1-anilinonaphthalene-8-sulfonate (ANS) was used as a probe of non-polar binding sites in 4-aminobutyrate aminotransferase. ANS binds to a single binding site of the dimeric protein with a Kd of 6 microM. Nanosecond emission anisotropy measurements were performed on the ANS-enzyme in an effort to d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80971-5
更新日期:1983-08-22 00:00:00
abstract::The Rad51 gene of Saccharomyces cerevisiae is required for genetic recombination and recombinational repair of DNA strand breaks. In higher eukaryotes Rad51 is essential for embryonic development, and is involved in cell proliferation and DNA repair. Here we show that human Rad51 (HsRad51) is proteolytically cleaved d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00433-5
更新日期:1998-05-08 00:00:00
abstract::The accumulation of pre-PhoS under conditions of PhoS overproduction has been previously described. It is now demonstrated that during the induction of PhoS, a delay in the completion of polypeptide chain elongation can be detected. This delay is related to the extent of jamming of export sites by pre-PhoS or by other...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80204-6
更新日期:1986-02-03 00:00:00
abstract::The release of a GPI-linked oncofetal protein (PLAP) from HEp-2 cells has been studied as an indicator of endogenous PI-specific PLC activation. A non-hydrolysable GTP analogue, EGF and insulin all produced a dose-dependent release of PLAP from these cells. This indicates that an increase in PLC activity following eit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80927-b
更新日期:1990-07-16 00:00:00
abstract::Dictyostelium discoideum Ax2 produces both L-erythro-tetrahydrobiopterin (BH4) and its stereoisomer D-threo-BH4 (DH4). The putative cofactor function of them for phenylalanine hydroxylase (PAH) was investigated through genetic manipulation and quantitative determination of pteridines. In addition to establishing that ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.08.026
更新日期:2011-10-03 00:00:00
abstract::The transcriptional control region of the Rouse sarcoma virus long terminal repeats (LTR) was shown to contain enhancer and promoter elements located within 200 base pairs upstream from the transcription initiation site [Cullen et al. (1985) Mol. Cell. Biol. 5, 438-447]. Deletion of these elements results in significa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01377-d
更新日期:1995-01-16 00:00:00
abstract::Jun activation domain-binding protein 1 (Jab1) is a coactivator of activating protein-1 (AP-1) and is the fifth component of the COP9 signalosome complex. It interacts with a variety of proteins and plays important roles in diverse signaling pathways and cellular function including oncogenesis. We show here that Jab1 ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.10.002
更新日期:2006-10-30 00:00:00
abstract::A recombinant expression system was developed to analyse sequence determinants involved in O-glycosylation of proteins in mycobacteria. By expressing peptide sequences corresponding to known glycosylation sites within a chimeric lipoprotein construct, amino acids flanking modified threonine residues were found to have...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01553-2
更新日期:2000-05-19 00:00:00
abstract::The myo-inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ mobilization in single saponin-permeabilized and fura-2-loaded parietal cells was analysed by a fluorescence digital image processor. When the cells were incubated with ATP, free cytoplasmic Ca2+ concentration [( Ca2+]i) increased in some restricted cytoplasmic r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80696-3
更新日期:1988-04-11 00:00:00
abstract::Misfolded or unassembled secretory proteins are retained in the endoplasmic reticulum (ER) and subsequently degraded by the cytosolic ubiquitin-proteasome system. This requires their retrograde transport from the ER lumen into the cytosol, which is mediated by the Sec61 translocon. It had remained a mystery whether ER...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01724-4
更新日期:1999-01-29 00:00:00
abstract::Studies were undertaken to further elucidate the mechanism(s) by which bradykinin-dependent phosphoinositide metabolism takes place in neuroblastoma X glioma hybrid NG108-15 cells [(1984) J. Biol. Chem. 259, 10201-10207] using [3H]inositol-labelled cells. Bradykinin produced net increases in the level of [3H]inositol ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80301-x
更新日期:1985-02-25 00:00:00
abstract::Glutamate uptake by rat hepatocytes in primary monolayer culture was found to be mediated by a Na+-independent and by two Na+-dependent transport systems of high and low affinity. Inhibition studies with cysteate and other model amino acids rules out the participation of the neutral amino acid transport systems A, ASC...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81024-2
更新日期:1983-09-19 00:00:00
abstract::We have studied functional properties of peptide transport in the pheochromocytoma neuroendocrine cell line from rat. The neutral peptide D-Phe-L-Ala (resistant to hydrolysis) is a good substrate for uptake into these cells. Transport is substantially inhibited by diethylpyrocarbonate pretreatment and is stimulated by...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03081-2
更新日期:2001-11-23 00:00:00
abstract::Specific receptors for angiotensin II (AII) were expressed in albino Xenopus laevis oocytes co-injected with poly(A)+ mRNA isolated from rat adrenal cortex and the calcium-specific photoprotein, aequorin. In such oocytes, AII elicited rapid, dose-dependent rises in cytosolic free calcium with light emission responses ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81055-x
更新日期:1988-12-05 00:00:00
abstract::Several neurodegenerative disorders are characterized by the accumulation of proteinaceous inclusions in the central nervous system. These inclusions are frequently composed of a mixture of aggregation-prone proteins. Here, we used a bimolecular fluorescence complementation assay to study the initial steps of the co-a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.11.019
更新日期:2012-01-02 00:00:00
abstract::This article presents an overview of the simulation studies of the behaviour of multihaem cytochromes using theoretical/computational methodologies, with an emphasis on cytochrome c(3). It starts with the first studies using rigid molecules and continuum electrostatic models, where protonation and redox events were tr...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2011.10.019
更新日期:2012-03-09 00:00:00
abstract::Abstract Type V adenylyl cyclase (AC) was stably over-expressed in HEK293 cells (293AC-V). Forskolin-stimulated cAMP accumulation in 293AC-V was 5 times as great as that in control cells. PMA, a protein kinase C (PKC) activator, enhanced cAMP accumulation in 293AC-V cells dose-and time-dependently and this enhancement...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00331-6
更新日期:1996-04-22 00:00:00
abstract::Growth of regenerating lung lobes is preceded by an increase of the activity of L-ornithine decarboxylase (ODC). Mechanical factors play a role in determining both the growth rate and enhancement of activity of ODC in regenerating lung lobes. Hormonal regulation also appears of importance as shown by the increased ODC...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80585-2
更新日期:1984-09-17 00:00:00
abstract::The sarcoplasmic reticulum-bound creatine kinase from rabbit skeletal muscle was inhibited by the nitric oxide donor S-nitrosoglutathione (GSNO). This led to a decrease in Ca2+ uptake in sarcoplasmic reticulum vesicles when the transport was driven by ATP generated from phosphocreatine and ADP. In contrast, the Ca 2+ ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00829-0
更新日期:1996-09-02 00:00:00
abstract::Absence through mutation of two proteins involved in periplasmic disulphide bond formation, DsbA and DsbB, results in failure of anaerobically grown Escherichia coli to synthesise the holo forms of either its endogenous c-type cytochrome nitrite reductase or exogenous cytochrome c550 from Paracoccus denitrificans. The...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01256-2
更新日期:1996-12-02 00:00:00
abstract::We have shown that OSCP, a subunit of yeast mitochondrial ATP synthase, can be incorporated into the intact enzyme as a fusion protein representing OSCP fused at its C-terminus to the green fluorescent protein (GFP) of Aequorea victoria. The relevant fusion OSCP-GFP-h6 additionally contains a hexahistidine tag at the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00670-4
更新日期:1997-07-07 00:00:00
abstract::The possibility that the phospholipid-N-methyltransferases from yeast are capable of acting upon a phospholipid substrate, localized in a different membrane than in which the enzymes reside ('trans-catalysis' hypothesis), was investigated using cho2 and opi3 gene disruptant strains, which are defective in phosphatidyl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02298-6
更新日期:2002-02-27 00:00:00
abstract::Gibberellins are ent-kaurene-derived diterpenoid phytohormones produced by plants, fungi, and bacteria. The distinct gibberellin biosynthetic pathways in plants and fungi are known, but not that in bacteria. Plants typically use two diterpene synthases to form ent-kaurene, while fungi use only a single bifunctional di...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.12.052
更新日期:2009-01-22 00:00:00
abstract::Pioneer crystallographic studies of the isolated 30S ribosomal subunit provided the first structural insights into the decoding process. Recently, new crystallographic data on full 70S ribosomes with mRNA and tRNAs have shown that the formation of the tight decoding centre is ensured by conformational rearrangement of...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2013.05.009
更新日期:2013-06-27 00:00:00
abstract::The formation of cytoplasmic inclusion bodies by Escherichia coli overproducing Clostridium thermocellum endoglucanase D (EGD) was investigated. EGD was found in inclusion bodies as a 68 kDa form, whereas the size of the cytoplasmic form was 65 kDa. Upon solubilization with urea followed by dialysis, the 68 kDa form w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80478-l
更新日期:1991-04-22 00:00:00
abstract::Two genes code for calcitonin gene-related peptides (CGRPs). One expresses by tissue-specific alternate splicing calcitonin and CGRP I mRNAs, the other CGRP II mRNA. Calcitonin is the marker of sporadic or hereditary human medullary thyroid carcinoma (MTC). CGRP II expression is not well established in normal or tumor...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80856-e
更新日期:1990-12-17 00:00:00
abstract::Macrophages play vital roles in inflammatory responses, and their number at sites of inflammation is strictly regulated by cell death and division. Here, we demonstrate that production of nitric oxide (NO) is a major mechanism whereby ceramide-1-phosphate (C1P) blocks apoptosis in macrophages. However, NO failed to st...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.05.027
更新日期:2008-06-25 00:00:00
abstract::The mutation of Asp198 to Asn in the receptor for glucagon-like peptide-1(7-36)amide (GLP-1) had no effect upon GLP-1 affinity whereas substitution with Ala greatly reduced affinity, demonstrating the importance of polarity rather than negative charge at Asp198. However, the Asp198-Ala mutation had less effect upon th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03492-0
更新日期:2002-10-23 00:00:00