Abstract:
:Mature beta-hexosaminidase A has been found associated to the external leaflet of plasma membrane of cultured fibroblasts. The plasma membrane association of beta-hexosaminidase A has been directly determined by cell surface biotinylation followed by affinity chromatography purification of the biotinylated proteins, and by immunocytochemistry. The immunological and biochemical characterization of biotinylated beta-hexosaminidase A revealed that the plasma membrane associated enzyme is fully processed, suggesting its lysosomal origin.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Mencarelli S,Cavalieri C,Magini A,Tancini B,Basso L,Lemansky P,Hasilik A,Li YT,Chigorno V,Orlacchio A,Emiliani C,Sonnino Sdoi
10.1016/j.febslet.2005.08.081keywords:
subject
Has Abstractpub_date
2005-10-24 00:00:00pages
5501-6issue
25eissn
0014-5793issn
1873-3468pii
S0014-5793(05)01107-5journal_volume
579pub_type
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