Receptors for mammalian steroid hormones in microbes and plants.

abstract：:Recent studies show LDL receptor-related protein 1B, LRP1B as a transducer of extracellular signals. Here, we identify six interacting partners of the LRP1B cytoplasmic region by yeast two-hybrid screen and confirmed their in vivo binding by immunoprecipitation. One of the partners, PICK1 recognizes the C-terminus of ...

journal_title：FEBS letters

authors： Shiroshima T,Oka C,Kawaichi M

更新日期：2009-01-05 00:00:00

abstract：:Undifferentiated rat pheochromocytoma PC12 cells were voltage clamped using the whole cell technique. After blockade of outward currents, calcium currents were elicited from -40 and -100 mV. A subpopulation of cells displayed only one current component activated at -10 mV and slowly decaying. In other cells this curre...

journal_title：FEBS letters

authors： Janigro D,Maccaferri G,Meldolesi J

更新日期：1989-09-25 00:00:00

abstract：:In Drosophila, the tumor suppressor Scribble is localized at the septate junctions of epithelial cells. Its mammalian homologue, hScrib, is a basolateral protein likely associated to proteins of the cell-cell junctions. We report the direct interaction between hScrib and ZO-2, a junction-associated protein. This inter...

journal_title：FEBS letters

authors： Métais JY,Navarro C,Santoni MJ,Audebert S,Borg JP

更新日期：2005-07-04 00:00:00

abstract：:Most tumor cells attain their immortality by reactivating telomerase. We report here the telomerase inhibitory potential of chimeric oligonucleotides composed of a 13mer antisense sequence targeting the telomerase RNA template region and a (s4dU)n moiety at its 3' or 5'-end. The increase of the thiolated chain length ...

journal_title：FEBS letters

authors： Tarkanyi I,Horváth A,Szatmari I,Eizert H,Vámosi G,Damjanovich S,Ségal-Bendirdjian E,Aradi J

更新日期：2005-02-28 00:00:00

abstract：:Nitrogenase is a two-component metalloenzyme that catalyzes a MgATP hydrolysis driven reduction of substrates. Aluminum fluoride plus MgADP inhibits nitrogenase by stabilizing an intermediate of the on-enzyme MgATP hydrolysis reaction. We report here the redox properties and electron paramagnetic resonance (EPR) signa...

journal_title：FEBS letters

authors： Spee JH,Arendsen AF,Wassink H,Marritt SJ,Hagen WR,Haaker H

更新日期：1998-07-31 00:00:00

abstract：:Two different clones of Swiss 3T3 cells belonging to the same original cell line have been obtained, one of which was unresponsive to mitogenic stimulation (e.g. insulin-like growth factor-I, bombesin, insulin-like growth factor-I + bombesin), while the other clone showed a very high rate of DNA synthesis under identi...

journal_title：FEBS letters

authors： Martelli AM,Gilmour RS,Neri LM,Manzoli L,Corps AN,Cocco L

更新日期：1991-06-03 00:00:00

abstract：:We used a xenograft model to investigate whether the aryl hydrocarbon receptor deletion construct CDelta553 suppresses tumor growth. HeLa cells that were infected with CDelta553 expressing adenovirus (Ad553) formed very small tumors whereas the control adenovirus-infected cells formed large tumors at day 15. CDelta553...

journal_title：FEBS letters

authors： Wang D,Faridi JS,Li Y,Chan WK

更新日期：2009-09-17 00:00:00

abstract：:The relationship between glutathione deficiency, glycogen metabolism and ascorbate synthesis was investigated in isolated murine hepatocytes. Glutathione deficiency caused by various agents increased ascorbate synthesis with a stimulation of glycogen breakdown. Increased ascorbate synthesis from UDP-glucose or gulonol...

journal_title：FEBS letters

authors： Braun L,Csala M,Poussu A,Garzó T,Mandl J,Bánhegyi G

更新日期：1996-06-17 00:00:00

abstract：:Tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF family of cytokines that promotes apoptosis and NF-kappaB activation. Here we show that recombinant hu-TRAIL initiates the activation of multiple caspases, the loss of mitochondrial transmembrane potential, the cleavage of BI...

journal_title：FEBS letters

authors： Keogh SA,Walczak H,Bouchier-Hayes L,Martin SJ

更新日期：2000-04-07 00:00:00

abstract：:In this work we have investigated the role of specific peptide sequences for glucose-inactivation of the yeast isocitrate lyase. Thus, different fragments of the ICL1 coding region were fused to the lacZ gene of E. coli to provide a reporter construction. Determinations of beta-galactosidase activities indicated that ...

journal_title：FEBS letters

authors： Ordiz I,Herrero P,Rodicio R,Moreno F

更新日期：1995-07-03 00:00:00

abstract：:Changes in membrane expression of sodium-dependent glucose transporter (SGLT1) and glucose transporter isoform (GLUT2) protein have been implicated in the increased intestinal glucose transport in streptozotocin-diabetes. The possible involvement of GLUT1 in the transport response, however, has not previously been stu...

journal_title：FEBS letters

authors： Boyer S,Sharp PA,Debnam ES,Baldwin SA,Srai SK

更新日期：1996-11-04 00:00:00

abstract：:DNA is very stable in its double-stranded form. For many processes of DNA metabolism, such as replication, repair, recombination and transcription, the DNA has to be brought transiently into a single-stranded form. DNA helicases are enzymes capable of melting the hydrogen bonds of base pairs by using the energy of nuc...

journal_title：FEBS letters

authors： Thömmes P,Hübscher U

更新日期：1990-08-01 00:00:00

abstract：:Contribution of phosphatidic acid (PA) generated by activated phospholipase (PL) D to PLA2 activation was studied in rat peritoneal mast cells. Exogenous didecanoyl PA induced arachidonate liberation in the permeabilized cells which was inhibited by p-bromophenacyl bromide. Upon exposure of the cells to ethanol in a h...

journal_title：FEBS letters

authors： Sato T,Ishimoto T,Akiba S,Fujii T

更新日期：1993-05-24 00:00:00

abstract：:The class A Ca2+ channel alpha 1 subunit (alpha 1A) was expressed in Xenopus oocytes alone or in combination with the beta 1b, beta 2a, beta 3, or beta 4 subunit. Analysis of voltage-dependent activation and inactivation in the presence of 1.8 mM external Ca2+ showed an hyperpolarising shift of both relations when com...

journal_title：FEBS letters

authors： Cens T,Mangoni ME,Nargeot J,Charnet P

更新日期：1996-08-12 00:00:00

abstract：:Niemann-Pick C (NPC) disease is a progressive neurological disorder in which cholesterol, gangliosides and bis-monoacylglycerol phosphate accumulate in late endosomes/lysosomes. This disease is caused by mutations in either the NPC1 or NPC2 gene. NPC1 and NPC2 are involved in egress of lipids, particularly cholesterol...

journal_title：FEBS letters

authors： Vance JE

更新日期：2006-10-09 00:00:00

abstract：:The C1 domains are 50 amino acid sequences present in protein kinase C (PKC) isozymes that are responsible for binding of phorbol esters and the lipid second messenger diacylglycerol (DAG). We found that bacterially expressed C1b domain of PKCdelta induces germinal vesicle breakdown (GVBD) when microinjected into Xeno...

journal_title：FEBS letters

authors： Aroca P,Santos E,Kazanietz MG

更新日期：2000-10-13 00:00:00

abstract：:Sterically hindered cyclic amines react with hydroxyl radicals in the presence of oxygen to yield stable nitroxide radicals which can be detected by EPR. This reaction provides a nonconventional spin-trapping tool for detection of hydroxyl radicals. ...

journal_title：FEBS letters

authors： Rosenthal I,Krishna CM,Yang GC,Kondo T,Riesz P

更新日期：1987-09-28 00:00:00

abstract：:To characterize energy metabolism in rat brown adipose tissue (BAT), we carried out differential screening of a cDNA library of BAT with a cDNA probe of white adipose tissue (WAT) and isolated one cDNA clone. It contained a single open reading frame of 2,316 bases which encodes a protein of 88.2 kDa. The predicted ami...

journal_title：FEBS letters

authors： Yamazaki N,Shinohara Y,Shima A,Terada H

更新日期：1995-04-17 00:00:00

abstract：:The mitochondrial alternative oxidase is a diiron carboxylate quinol oxidase (Dox) found in plants and some fungi and protists, but not animals. The plastid terminal oxidase is distantly related to alternative oxidase and is most likely also a Dox protein. Database searches revealed that the alpha-proteobacterium Novo...

journal_title：FEBS letters

authors： Finnegan PM,Umbach AL,Wilce JA

更新日期：2003-12-18 00:00:00

abstract：:The mutant hen egg white lysozymes Ile55Thr and Asp66His, corresponding to human amyloidogenic mutant lysozymes Ile56Thr and Asp67His, respectively, were secreted in Saccharomyces cerevisiae. The amyloidogenic mutants (I55T and D66H) of hen egg white lysozymes were remarkably less soluble than that of the wild-type pr...

journal_title：FEBS letters

authors： Song Y,Azakami H,Hamasu M,Kato A

更新日期：2001-02-23 00:00:00

abstract：:The phosphatidylinositol 3-kinase/Akt pathway is responsible for key aspects of tumor progression, and is frequently hyperactivated in cancer. We have recently identified palladin, an actin-bundling protein that functions to control the actin cytoskeleton, as an Akt1-specific substrate that inhibits breast cancer cell...

journal_title：FEBS letters

authors： Chin YR,Toker A

更新日期：2010-12-01 00:00:00

abstract：:Seven peptides from subunit 9 (S9) of the human 26S proteasome were sequenced and this information was used to clone a HeLa cDNA that encodes the 46 kDa subunit. Rabbit polyclonal antisera were made against a ubiquitin fusion protein containing 12 amino acids from S9 and against a full-length S9 expressed in E. coli. ...

journal_title：FEBS letters

authors： Hoffman L,Rechsteiner M

更新日期：1997-03-10 00:00:00

abstract：:A selective PKC inhibitor, UCN-01, was shown to exhibit anti-tumor activity in vitro and in vivo. We investigated UCN-01 with respect to isozyme-specific PKC inhibition using purified recombinant or rabbit brain PKC isozymes, cPKC alpha, beta and gamma, nPKC delta, epsilon and eta, and a PKC zeta. Of the PKC isozymes ...

journal_title：FEBS letters

authors： Mizuno K,Noda K,Ueda Y,Hanaki H,Saido TC,Ikuta T,Kuroki T,Tamaoki T,Hirai S,Osada S

更新日期：1995-02-13 00:00:00

abstract：:Association of protein inclusions or aggregates within brain tissues of patients with neurodegenerative disorders has been widely reported. These inclusions are commonly characterised both by the presence of ubiquitylated proteins and the sequestration of components of the ubiquitin-proteasome system (UPS). Such obser...

journal_title：FEBS letters

authors： Ardley HC,Hung CC,Robinson PA

更新日期：2005-01-31 00:00:00

abstract：:ATM (ataxia telangiectasia mutated), the gene mutated in ataxia telangiectasia, is related to a family of large phosphatidylinositol 3-kinase domain-containing proteins involved in cell cycle control and DNA repair. We found that ATM(-/-) DT40 cells were more susceptible than wild-type cells to apoptosis induced not o...

journal_title：FEBS letters

authors： Takao N,Li Y,Yamamoto K

更新日期：2000-04-21 00:00:00

abstract：:Tau proteins aggregate into different neuronal inclusions in several neurodegenerative disorders. In Alzheimer's disease (AD), hyperphosphorylated Tau from paired helical filaments (PHF) of neurofibrillary tangles, named PHF-Tau, have an electrophoretic profile with four main bands (Tau 55, 64, 69, 74 kDa). In Pick's ...

journal_title：FEBS letters

authors： Sergeant N,David JP,Lefranc D,Vermersch P,Wattez A,Delacourte A

更新日期：1997-08-04 00:00:00

abstract：:Cathepsin L is a ubiquitously expressed papain-like cysteine protease involved in the endosomal degradation of proteins and has numerous roles in physiological and pathological processes, such as arthritis, osteoporosis, and cancer. Insight into the specificity of cathepsin L is important for elucidating its physiolog...

journal_title：FEBS letters

authors： Sosnowski P,Turk D

更新日期：2016-04-01 00:00:00

abstract：:In this study, we demonstrated that tissue inhibitor of metalloproteinases (TIMP) produced by human bone marrow stromal cell line KM-102 had erythroid-potentiating activity (EPA) which stimulates the proliferation of erythroid progenitor cells. We, then, propose a scheme for the bifunctional role of TIMP/EPA in hemato...

journal_title：FEBS letters

authors： Hayakawa T,Yamashita K,Kishi J,Harigaya K

更新日期：1990-07-30 00:00:00

abstract：:Saccharomyces cerevisiae has only one gene encoding a putative voltage-gated Ca2+ channel pore-forming subunit, CCH1, which is not possible to be cloned by conventional molecular cloning techniques using Escherichia coli. Here, we report the successful cloning of CCH1 in yeast by in vivo homologous recombination witho...

journal_title：FEBS letters

authors： Iida K,Tada T,Iida H

更新日期：2004-10-22 00:00:00

abstract：:The dual function of the regulatory beta-subunit of protein kinase CK2 is highlighted by its ability to abolish calmodulin phosphorylation in contrast to its stimulatory effect on the phosphorylation of peptide substrates. Here we show that a synthetic peptide reproducing the C-terminal region of the beta-subunit (bet...

journal_title：FEBS letters

authors： Marin O,Meggio F,Boldyreff B,Issinger OG,Pinna LA

更新日期：1995-04-17 00:00:00