Abstract:
:The nature of the G-proteins present in the pre- and post-synaptic plasma membranes and in the synaptic vesicles of cholinergic nerve terminals purified from the Torpedo electric organ was investigated. In pre- and post-synaptic plasma membranes, Bordetella pertussis toxin, known to catalyze the ADP-ribosylation of the alpha-subunit of several G-proteins, labels two substrates at 41 and 39 kDa. The 39 kDa subunit detected by ADP-ribosylation in the synaptic plasma membrane fractions was immunologically similar to the Go alpha-subunit purified from calf brain. In contrast to bovine chromaffin cell granules, no G-protein could be detected in Torpedo synaptic vesicles either by ADP-ribosylation or by immunoblotting.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Toutant M,Bockaert J,Homburger V,Rouot Bdoi
10.1016/0014-5793(87)80190-4subject
Has Abstractpub_date
1987-09-28 00:00:00pages
51-5issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(87)80190-4journal_volume
222pub_type
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