Abstract:
:Proteins with a CyaB, thiamine triphosphatase domain (CYTH domain) may play a central role at the interface between nucleotide and polyphosphate metabolism. One of the plant CYTH domain-containing proteins from Brachypodium distachyon, BdTTM3, is annotated in NCBI databases as an 'adenylyl cyclase (AC)' or a 'triphosphate tunnel metalloenzyme'. The divergent nomenclature and the search for plant ACs induced us to experimentally confirm the enzymatic activity of BdTTM3. Based on in vitro analysis, we have shown that the recombinant form of BdTTM3 is a protein with high triphosphatase activity (binding both tripolyphosphate and ATP) and low AC activity. Furthermore, the analysis of BdTTM3 transcriptional activity indicates its involvement in the mechanism underlying responses to wounding stress in B. distachyon leaves.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Świeżawska B,Duszyn M,Kwiatkowski M,Jaworski K,Pawełek A,Szmidt-Jaworska Adoi
10.1002/1873-3468.13701subject
Has Abstractpub_date
2020-03-01 00:00:00pages
1101-1111issue
6eissn
0014-5793issn
1873-3468journal_volume
594pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Selenium depletion of H4 hepatoma cells reduced cytosolic glutathione peroxidase (cGSH-Px) mRNA abundance but had no effect on phospholipid hydroperoxide glutathione peroxidase (PHGSH-Px) mRNA abundance. Actinomycin D chase experiments showed that selenium depletion had no effect on the stability of PHGSH-Px mRNA but ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00493-0
更新日期:1996-06-03 00:00:00
abstract::The cytochrome P-450 content of rat hepatocytes declined rapidly over 72 h in culture, due primarily to denaturation to cytochrome P-420. Six different media were investigated for their ability to conserve cytochrome P-450 during culture, and the most successful was a modified Earle's medium. After 72 h culture in thi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80436-1
更新日期:1985-10-07 00:00:00
abstract::Hydrolysis of GTP, bound to members of the G-protein superfamily, terminates their downstream signaling activity. A conserved glutamine serves a critical role in this pivotal guanosine triphosphatase (GTPase) reaction. However, the role of the catalytic glutamine in GTP hydrolysis is still not well understood. We have...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00930-2
更新日期:1998-08-21 00:00:00
abstract::Ataxin-3, which is encoded by a gene that has been associated with Machado-Joseph disease, contains a catalytic N-terminal Josephin domain with deubiquitinase activity. Here, we show that the Josephin domain of ataxin 3 catalyzes endo-type cleavage of Lys48-linked polyubiquitin. Furthermore, NMR data obtained followin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.10.013
更新日期:2014-11-28 00:00:00
abstract::Yeasts with disruptions in the genes PYC1 and PYC2 encoding the isoenzymes of pyruvate carboxylase cannot grow in a glucose-ammonium medium (Stucka et al. (1991) Mol. Gen. Genet. 229, 307-315). We have isolated a dominant mutation, BPC1-1, that allows growth in this medium of yeasts with interrupted PYC1 and PYC2 gene...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01337-7
更新日期:1995-12-18 00:00:00
abstract::32P-Labelled washed rabbit platelets were incubated with 0.6 nM platelet activating factor (PAF-acether), giving a full aggregation and release response within 30-60 s. The major phospholipid changes observed under these conditions were: (1) An increased labelling of phosphatidic acid (PA) within 10 s and of phosphati...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80643-7
更新日期:1983-03-21 00:00:00
abstract::The interaction of Fas (Apo-1) and TNF receptor-1 with their respective ligands can lead to cell death. The so-called death domain, a sequence motif present in the cytoplasmic portion of the two receptors, has been identified as a critical structural element involved in signal transduction that leads to apoptosis. Her...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00931-x
更新日期:1995-09-11 00:00:00
abstract::The E-E* model for calcium pumping by the CaATPase of sarcoplasmic reticulum includes two distinct conformational states of the enzyme, E and E*. Exterior Ca2+ binds only to E and interior Ca2+ binds only to E*. Therefore, it is expected that there will be competition between the binding of calcium to the unphosphoryl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80077-g
更新日期:1991-01-14 00:00:00
abstract::A long-term goal of the field of interactome modeling is to understand how global and local properties of complex macromolecular networks impact on observable biological properties, and how changes in such properties can lead to human diseases. The information available at this stage of development of the field provid...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2005.02.030
更新日期:2005-03-21 00:00:00
abstract::To identify the novel genes involved in lipid metabolism and lipid droplet formation that may play important roles in Hepatitis C virus (HCV) propagation, we have screened the small interfering RNA library using cell culture derived HCV (HCVcc)-infected cells. We selected and characterized the gene encoding farnesyl-d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.03.043
更新日期:2014-05-02 00:00:00
abstract::The alkaline phosphatase of beef, horse, pig and sheep brain have been purified. In each case, the alkaline phosphatase activity can be separated into two or three components by DEAE-cellulose chromatography and by electrophoresis. Although the properties of these enzyme fractions are very similar, there are sufficien...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(70)80073-4
更新日期:1970-02-16 00:00:00
abstract::Tomato Cf-9, a receptor-like protein (RLP), confers resistance to races of the fungal pathogen Cladosporium fulvum that express the Avr9 avirulence gene. CITRX (Cf-9-interacting thioredoxin) was previously identified in a yeast two-hybrid screen as a protein interacting with the cytoplasmic domain of Cf-9 and shown to...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.06.077
更新日期:2006-07-24 00:00:00
abstract::The mitochondrial alternative oxidase is a diiron carboxylate quinol oxidase (Dox) found in plants and some fungi and protists, but not animals. The plastid terminal oxidase is distantly related to alternative oxidase and is most likely also a Dox protein. Database searches revealed that the alpha-proteobacterium Novo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01309-7
更新日期:2003-12-18 00:00:00
abstract::We have cloned and characterized four Itpk genes from soybean. All four recombinant Itpk proteins showed canonical Ins(1,3,4)P3 5/6-kinase activity, but a kinetic analysis raised questions about its biological significance. Instead, we provide evidence that one alternative biological role for soybean Itpks is to inter...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.04.054
更新日期:2008-06-11 00:00:00
abstract::Nuclear magnetic resonance (NMR) spectroscopy was used to determine the secondary structure and the three-dimensional polypeptide backbone fold of the human sterol carrier protein 2 (hSCP2), which is a basic protein with 123 residues believed to participate in the intracellular transport of cholesterol and various oth...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80431-s
更新日期:1993-11-29 00:00:00
abstract::The transmembrane protein MIG-13 is a key regulator required for anterior migration of neural cells in Caenorhabditis elegans, but the signaling mechanisms involved remain unknown. Here, we isolated a suppressor mutation in the unc-71/adm-1 gene, which rescued the AVM neuron migration defect in mig-13 mutants. Genetic...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.01.031
更新日期:2012-03-23 00:00:00
abstract::Snake venoms are rich sources of serine proteinase inhibitors that are members of the Kunitz/BPTI (bovine pancreatic trypsin inhibitor) family. However, only a few of their gene sequences have been determined from snakes. We therefore cloned the cDNAs for the trypsin and chymotrypsin inhibitors from a Vipera ammodytes...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00693-8
更新日期:2003-07-17 00:00:00
abstract::Phosphotyrosine-containing proteins in Dictyostelium discoideum were detected by immunoblot analysis and immunoprecipitation using a monoclonal anti-phosphotyrosine antibody. The iodinated antibody recognized on bots a cluster of 205-220 kDa polypeptides and bands of 107 and 60 kDa. The 107 and 60 kDa polypeptides and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81007-b
更新日期:1990-07-30 00:00:00
abstract::New polypeptide components have been isolated from Dendroaspis angusticeps venom using chromatography. Two polypeptides containing 59 and 57 amino acids, called 'DaE1' and 'DaE2' respectively, have been purified to homogeneity and fully sequenced. Spectrometric analysis yielded masses of 6631.5 and 6389.0 Da, respecti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02201-3
更新日期:2001-03-02 00:00:00
abstract::The trichothecene 3-O-acetyltransferase gene, Tri101, plays a pivotal role for the well-being of the type B trichothecene producer Fusarium graminearum. We have analyzed the cosmids containing Tri101 and found that this resistance gene is not in the biosynthetic gene cluster reported so far. It was located between the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01061-8
更新日期:1998-09-18 00:00:00
abstract::The 50 S subunit proteins from the Escherichia coli ribosome were purified by size-exclusion, ion-exchange or reversed phase high performance liquid chromatography (HPLC) avoiding any precipitation or desalting procedures during isolation. Best resolution of this complex protein mixture was achieved by reversed phase ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80832-7
更新日期:1984-02-13 00:00:00
abstract::Monoclonal H5 antibodies and a polyclonal antiserum, raised against the globular domain of chicken H5 (GH5) but which cross-reacts with histone H1(0) from mouse liver, were used to search for H5 or H1 (0)-like proteins in chicken embryo and adult tissue sections by indirect immunofluorescence. Chicken cell lines in cu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80704-3
更新日期:1985-07-08 00:00:00
abstract::Extended X-ray absorption fine structure (EXAFS) spectra at the Cu K-edge have been recorded of the oxidized and reduced form at pH 3.5 of rusticyanin, the type-1 or 'blue'-copper protein from Thiobacillus ferrooxidans. The EXAFS of oxidized rusticyanin is well simulated with models assuming a ligand set of 2 N(His) a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81133-9
更新日期:1990-08-20 00:00:00
abstract::Effects of sulfatide and its precursor galactosylceramide (gal-cer) on the kinetics of production of cytokines were studied. In human mononuclear leucocytes, gal-cer but not sulfatide induced significantly increased amounts of interleukin (IL)-1beta, IL-6 and tumor necrosis factor (TNF) mRNA. In phytohemagglutinin-sti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00908-4
更新日期:1999-07-23 00:00:00
abstract::The dgd1 mutant of Arabidopsis thaliana provides us with a powerful tool for revealing the specific role of digalactosyldiacylglycerol (DGDG) in photosynthesis. Blue-native polyacrylamide gel electrophoresis analysis revealed that photosystem I (PSI) subunits are assembled into a PSI complex, and that a PSI subcomplex...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.05.049
更新日期:2005-07-04 00:00:00
abstract::Abstract Type V adenylyl cyclase (AC) was stably over-expressed in HEK293 cells (293AC-V). Forskolin-stimulated cAMP accumulation in 293AC-V was 5 times as great as that in control cells. PMA, a protein kinase C (PKC) activator, enhanced cAMP accumulation in 293AC-V cells dose-and time-dependently and this enhancement...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00331-6
更新日期:1996-04-22 00:00:00
abstract::Syntabulin is a microtubule-associated protein that mediates anterograde transport of vesicles to neuronal processes. Here, we found that syntabulin was expressed in mouse pancreas and insulin-secreting β-cells, and that it partially co-localized with microtubule and insulin-containing granules. The association of syn...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.08.025
更新日期:2012-10-19 00:00:00
abstract::Mitochondria continually fuse and divide to dynamically adapt to changes in metabolism and stress. Mitochondrial dynamics are also required for mitochondrial DNA (mtDNA) integrity; however, the underlying reason is not known. In this study, we examined the link between mitochondrial fusion and mtDNA maintenance in Sch...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12432
更新日期:2016-10-01 00:00:00
abstract::This study demonstrated that activation of tyrosine kinase of epidermal growth factor receptor (EGFR) induces its association with G protein-coupled receptor kinase 2 (GRK2). Immunoprecipitation experiments showed that EGF stimulation increased GRK2 binding to EGFR complex in HEK293 cells coexpressing EGFR and GRK2. T...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.11.057
更新日期:2005-01-03 00:00:00
abstract::Metabolic control theory is extended to include channelled metabolism in general. A simple relationship between the flux control by the enzymes and the degree of metabolite channelling is derived. This relationship suggests experiments in which modulation of gene expression allows one to quantify channelling. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81660-r
更新日期:1993-03-29 00:00:00