Mode of substrate recognition by the Josephin domain of ataxin-3, which has an endo-type deubiquitinase activity.

abstract：:The present study was focused on the regulation of ecdysteroidogenesis in the Y-organ of Scylla serrata during molting cycle. A strong expression of molt-inhibiting hormone (MIH) and phosphorylation of ERK was predominantly observed in the postmolt and intermolt stages of Y-organs, whereas protein kinase C, steroidoge...

journal_title：FEBS letters

authors： Imayavaramban L,Dhayaparan D,Devaraj H

更新日期：2007-11-13 00:00:00

abstract：:An essential component of the chromatin remodeling machinery is NURF (Nucleosome Remodeling Factor), the founding member of the ISWI family of chromatin remodeling complexes. In vertebrates and invertebrates alike, NURF has many important functions in chromatin biology including regulating transcription, establishing ...

journal_title：FEBS letters

authors： Alkhatib SG,Landry JW

更新日期：2011-10-20 00:00:00

abstract：:Bovine heart cytochrome-c oxidase was reconstituted in liposomes and the kinetics of cytochrome c oxidation were measured by the polarographic and photometric method under uncoupled conditions in the presence of various polyvalent anions. In order to distinguish between specific and unspecific ionic effects of ATP, th...

journal_title：FEBS letters

authors： Hüther FJ,Kadenbach B

更新日期：1986-10-20 00:00:00

abstract：:The relationship between glutathione deficiency, glycogen metabolism and ascorbate synthesis was investigated in isolated murine hepatocytes. Glutathione deficiency caused by various agents increased ascorbate synthesis with a stimulation of glycogen breakdown. Increased ascorbate synthesis from UDP-glucose or gulonol...

journal_title：FEBS letters

authors： Braun L,Csala M,Poussu A,Garzó T,Mandl J,Bánhegyi G

更新日期：1996-06-17 00:00:00

abstract：:To characterize energy metabolism in rat brown adipose tissue (BAT), we carried out differential screening of a cDNA library of BAT with a cDNA probe of white adipose tissue (WAT) and isolated one cDNA clone. It contained a single open reading frame of 2,316 bases which encodes a protein of 88.2 kDa. The predicted ami...

journal_title：FEBS letters

authors： Yamazaki N,Shinohara Y,Shima A,Terada H

更新日期：1995-04-17 00:00:00

abstract：:The role of two positively charged amino acid residues located at the active site of Escherichia coli maltodextrin phosphorylase was investigated by site-directed mutagenesis. Substitution of Lys539 by an arginine caused a 600-fold reduction, substitution of Arg534 by a glutamine caused an even larger 7000-fold reduct...

journal_title：FEBS letters

authors： Schinzel R,Drueckes P

更新日期：1991-07-29 00:00:00

abstract：:We have identified and purified a DNA binding protein which specifically binds to a segment of the mouse alpha 2 (I) collagen promoter between -420 and -399 bp upstream of the start of transcription. Purification included heparin-agarose and sequence-specific DNA-affinity chromatography, followed by SDS-PAGE and renat...

journal_title：FEBS letters

authors： Hatamochi A,de Crombrugghe B,Krieg T

更新日期：1993-08-02 00:00:00

abstract：:The plant toxin ricin has proven valuable as a membrane marker in studies of endocytosis as well as studies of different intracellular transport steps. The toxin, which consists of two polypeptide chains, binds by one chain (the B-chain) to both glycolipids and glycoproteins with terminal galactose at the cell surface...

journal_title：FEBS letters

authors： Sandvig K,van Deurs B

更新日期：1999-06-04 00:00:00

abstract：:Glucocorticoid-induced TNFR-related protein (GITR) is constitutively expressed in T regulatory (Treg) cells and regulates their suppressive function. We identified two methylated CpG islands in the Gitr locus. Using a ChIP assay, we demonstrate that both DNMT1 and methyl-CpG-binding domain Protein 4 (MBD4) bind to the...

journal_title：FEBS letters

authors： Wang S,Li Y,Zhu F,Lin F,Luo X,Zhao B,Zhang P,Li D,Gao Y,Liang R,Liu L,Tsun A,Yuan X,Wu K,Li B

更新日期：2017-07-01 00:00:00

abstract：:A mutant of Escherichia coli has been described that produces an altered form of penicillin-binding protein 5 which still binds penicillin but is unable to catalyse the release of the bound penicilloyl moiety. We show that the mutation is caused by a single nucleotide transition that results in a change from glycine a...

journal_title：FEBS letters

authors： Broome-Smith J,Spratt BG

更新日期：1984-01-09 00:00:00

abstract：:Incubation of rat adipocytes with the phorbol ester TPA caused a dose-dependent, persistent increase in phosphatidate phosphohydrolase activity and decrease in fatty acyl-CoA synthetase activity. Half-maximal effects were obtained with 1-2 X 10(-10) M TPA. TPA did not alter basal or noradrenaline-stimulated lipolysis....

journal_title：FEBS letters

authors： Hall M,Taylor SJ,Saggerson ED

更新日期：1985-01-07 00:00:00

abstract：:A novel peptide from Centruroides noxius Hoffmann scorpion venom was isolated and sequenced. The 37 amino acid peptide belongs to the charybdotoxin sub-family (alphaKTx1) and was numbered member 11. alphaKTx1.11 has 75% sequence identity with iberiotoxin and 54% with charybdotoxin. alphaKTx1.11 revealed specificity fo...

journal_title：FEBS letters

authors： Garcia-Valdes J,Zamudio FZ,Toro L,Possani LD

更新日期：2001-09-21 00:00:00

abstract：:Activation of protein kinase C (PKC) in Rat-1 fibroblasts leads to rapid phosphorylation of an 80-kDa protein, a major substrate of PKC. Digitonin-permeabilized cells perfectly supported this early response. Introduction of a PKC pseudosubstrate peptide inhibited 80 kDa phosphorylation with an IC50 of 1 microM, while ...

journal_title：FEBS letters

authors： Eichholtz T,Alblas J,van Overveld M,Moolenaar W,Ploegh H

更新日期：1990-02-12 00:00:00

abstract：:We have expressed the cloned rat kappa-opioid receptor in human embryonic kidney 293 cells and studied the ability of kappa-selective ligands to inhibit adenylyl cyclase. In transfected 293 cells, activation of the kappa-opioid receptor by U50,488 and the dynorphins resulted in the inhibition of cAMP accumulation. The...

journal_title：FEBS letters

authors： Lai HW,Minami M,Satoh M,Wong YH

更新日期：1995-02-20 00:00:00

abstract：:It is well known that growth hormone (GH)-induced IGF-1 signaling plays a dominant role in postnatal muscle growth. Our previous studies have identified a growth factor, progranulin (PGRN), that is co-induced with IGF-1 upon GH administration. This result prompted us to explore the function of PGRN and its association...

journal_title：FEBS letters

authors： Hu SY,Tai CC,Li YH,Wu JL

更新日期：2012-09-21 00:00:00

abstract：:Complete [1H]NMR resonance assignments are presented for the second of the three zinc fingers from SW15 as it appears in both 1- and 2-finger constructs. Signals from finger 2 are unaffected by the presence or absence of finger 1, showing that the protein has a modular construction. The structure of finger 2 comprises...

journal_title：FEBS letters

authors： Neuhaus D,Nakaseko Y,Nagai K,Klug A

更新日期：1990-03-26 00:00:00

abstract：:We investigated transactivation by NANOG in regulating growth and differentiation factor 3 (GDF3) expression in NCCIT cells. GDF3 expression was affected by shRNA-mediated downregulation and by exogenous overexpression of NANOG specifically, as well as by retinoic acid-mediated differentiation. GDF3 transcription was ...

journal_title：FEBS letters

authors： Park SW,Lim HY,Do HJ,Sung B,Huh SH,Uhm SJ,Song H,Chung HJ,Kim JH,Kim NH,Kim JH

更新日期：2012-09-21 00:00:00

abstract：:alpha-Crystallin, the major protein of the ocular lens, is known to have extensive similarities to small heat shock proteins and to act as a molecular chaperone. The exposure of hydrophobic surfaces on alpha-crystallin was studied by fluorescence spectroscopy using the hydrophobic probe bis-ANS. Upon heating the prote...

journal_title：FEBS letters

authors： Das KP,Surewicz WK

更新日期：1995-08-07 00:00:00

abstract：:Hematopoietic progenitors have been shown to retain plasticity and switch lineages by appropriate stimuli. However, mature blood cells hardly showed such differentiation plasticity. In this paper, we tried to reprogram mature B cells into erythroid lineage by expressing various hematopoietic transcription factors. Amo...

journal_title：FEBS letters

authors： Sadahira K,Fukuchi Y,Kunimono H,Sakurai M,Ikeda Y,Okamoto S,Nakajima H

更新日期：2012-10-19 00:00:00

abstract：:In the presence of ADP, the molecular chaperones GroEL and GroES from Escherichia coli not only facilitated the refolding of various proteins, but also prevented their irreversible heat inactivation in vitro. Without nucleotides the refolding reactions were arrested by GroEL. Addition of GroES and ADP to the reaction ...

journal_title：FEBS letters

authors： Kawata Y,Nosaka K,Hongo K,Mizobata T,Nagai J

更新日期：1994-05-30 00:00:00

abstract：:Regulation of Na(+)-dependent glutamate transport was studied in isolated luminal and abluminal plasma membranes derived from the bovine blood-brain barrier. Abluminal membranes have Na(+)-dependent glutamate transporters while luminal membranes have facilitative transporters. This organization allows glutamate to be ...

journal_title：FEBS letters

authors： Hawkins RA,Simpson IA,Mokashi A,Viña JR

更新日期：2006-08-07 00:00:00

abstract：:The remarkable stability of c-myc during oogenesis contrasts with its degradation during the early developmental period in Xenopus laevis. Three evolutionary conserved motifs found in the 3'-untranslated region of Xenopus c-myc RNAs have been analyzed for a possible role in c-myc RNA degradation. No specific degradati...

journal_title：FEBS letters

authors： Tchang F,Vriz S,Méchali M

更新日期：1991-10-21 00:00:00

abstract：:Analysis of the amino acid sequence of bovine brain myelin proteolipid reveals not only extensive internal homology, but also homology with portions of the myelin basic protein, the peripheral nerve myelin protein, Po, and with the small proteolipid subunit of mitochondrial ATP synthase. These results suggest that the...

journal_title：FEBS letters

authors： Laursen RA,Samiullah M,Lees MB

更新日期：1983-09-05 00:00:00

abstract：:Poly(A)+ RNA isolated from post-mortem human hypothalami has been used to characterize the poly-protein precursors to vasopressin and oxytocin. Translation in a cell-free system and subsequent immuno-precipitation with antibodies raised against either vasopressin or neurophysin identified a product of Mr 19000 (prepro...

journal_title：FEBS letters

authors： Mohr E,Hillers M,Ivell R,Haulica ID,Richter D

更新日期：1985-11-25 00:00:00

abstract：:U46619 is a potent platelet agonist, its binding to the thromboxane A2 receptor resulting in Gq-binding protein-mediated responses; nevertheless, it is unable to cause platelet aggregation, unless released ADP is present. In this study we demonstrate that Gi activation is the step U46619 lacks to cause platelet aggreg...

journal_title：FEBS letters

authors： Pulcinelli FM,Pesciotti M,Pignatelli P,Riondino S,Gazzaniga PP

更新日期：1998-09-11 00:00:00

abstract：:The reversible folding/unfolding of a short peptide in solution is studied by molecular dynamics simulations. The effects of long-range electrostatic interactions are examined and found to be important both for the equilibrium between folded and unfolded states and the dynamics of the folding process. The neglect of l...

journal_title：FEBS letters

authors： Aqvist J

更新日期：1999-09-03 00:00:00

abstract：:Evidence has previously suggested that cellobiose:quinone oxidoreductase (CBQ) in cellulolytic cultures of Phanerochaete chrysosporium might be produced from cellobiose oxidase (CBO) by proteolytic cleavage. This study demonstrates that the ratio of CBO activity to (CBO + CBQ) activity declines with decreasing culture...

journal_title：FEBS letters

authors： Habu N,Samejima M,Dean JF,Eriksson KE

更新日期：1993-07-26 00:00:00

abstract：:The ABCG2 protein has a key role in the transport of a wide range of structurally dissimilar endo- and xenobiotics in the human body, especially in the tissue barriers and the metabolizing or secreting organs. The human ABCG2 gene harbors a high number of polymorphisms and mutations, which may significantly modulate i...

journal_title：FEBS letters

authors： Sarkadi B,Homolya L,Hegedűs T

更新日期：2020-10-04 00:00:00

abstract：:The interaction of bovine pancreatic trypsin inhibitor (BPTI) and bovine tryptase, which are co-localized in the same granules of bovine mast cells, has been analyzed at 30 degrees C in 0.1 M Tris-HCl, pH 8.0. The analysis has unravelled that the functional unit of bovine tryptase is formed of (at least) four binding ...

journal_title：FEBS letters

authors： Fiorucci L,Erba F,Coletta M,Ascoli F

更新日期：1995-04-17 00:00:00

abstract：:Recent studies have shown that p21-activated kinase 1 (Pak1) phosphorylates estrogen receptor-alpha (ER alpha) at Ser 305 and also promotes its transactivation function. Here, we sought to investigate whether substitution of serine 305 in ER with glutamic acid (ER alpha-S305E), which mimics the phosphorylation state, ...

journal_title：FEBS letters

authors： Balasenthil S,Barnes CJ,Rayala SK,Kumar R

更新日期：2004-06-04 00:00:00