Abstract:
:Poly(A)+ RNA isolated from post-mortem human hypothalami has been used to characterize the poly-protein precursors to vasopressin and oxytocin. Translation in a cell-free system and subsequent immuno-precipitation with antibodies raised against either vasopressin or neurophysin identified a product of Mr 19000 (prepro-vasopressin). A second less intense product of Mr 16500 was tentatively identified as prepro-oxytocin. A cDNA library derived from the human hypothalamic poly(A)+ RNA was screened for vasopressin and oxytocin-encoding cDNA using heterologous probes; clones encoding the two precursors were identified and found to be organized as their rat and bovine counterparts. Northern blot analysis shows that the mRNAs for the two prepro-hormones consist of approximately 840 (AVP) and approximately 700 (OT) nucleotides.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Mohr E,Hillers M,Ivell R,Haulica ID,Richter Ddoi
10.1016/0014-5793(85)80069-7subject
Has Abstractpub_date
1985-11-25 00:00:00pages
12-6issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(85)80069-7journal_volume
193pub_type
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