A novel partner for the GTP-bound forms of rho and rac.

abstract：:A set of inhibitors that are useful for distinction of three types of human cytosolic glutathione transferase is presented. The near-neutral transferase is inhibited most effectively by Cibacron blue (I50 = 0.05 microM), the acidic transferase by Cibacron blue (I50 = 0.5 microM), and the basic transferase by tributylt...

journal_title：FEBS letters

authors： Tahir MK,Guthenberg C,Mannervik B

更新日期：1985-02-25 00:00:00

abstract：:Polyclonal antibodies have been raised against two synthetic peptides reproducing the 48-64 and 353-369 sequences of CSK, a protein tyrosine kinase implicated in the down-regulation of src-related protein kinases. Both antibodies specifically recognize recombinant CSK and a CSK-related 49 kDa protein tyrosine kinase p...

journal_title：FEBS letters

authors： Brunati AM,Allee G,Marin O,Donella-Deana A,Cesaro L,Bougeret C,Fagard R,Benarous R,Fischer S,Pinna LA

更新日期：1992-11-30 00:00:00

abstract：:In Ruminococcus albus, 4-O-β-D-mannosyl-D-glucose phosphorylase (RaMP1) and β-(1,4)-mannooligosaccharide phosphorylase (RaMP2) belong to two subfamilies of glycoside hydrolase family 130. The two enzymes phosphorolyze β-mannosidic linkages at the nonreducing ends of their substrates, and have substantially diverse sub...

journal_title：FEBS letters

authors： Ye Y,Saburi W,Odaka R,Kato K,Sakurai N,Komoda K,Nishimoto M,Kitaoka M,Mori H,Yao M

更新日期：2016-03-01 00:00:00

abstract：:Plasma membrane H(+)-ATPase of the yeast Saccharomyces cerevisiae was isolated and purified in its two forms, the activated A-ATPase from glucose-metabolizing cells, and the basal-level B-ATPase from cells with endogenous metabolism only. Using two-dimensional gel electrophoretic analysis, we showed that both enzyme p...

journal_title：FEBS letters

authors： Lapathitis G,Tanfani F,Kotyk A,Bertoli E

更新日期：2001-09-07 00:00:00

abstract：:This study was undertaken to demonstrate the unique specificity of the chemokine receptor CXCR4 antagonist AMD3100. Calcium flux assays with selected chemokine/cell combinations, affording distinct chemokine receptor specificities, revealed no interaction of AMD3100 with any of the chemokine receptors CXCR1 through CX...

journal_title：FEBS letters

authors： Hatse S,Princen K,Bridger G,De Clercq E,Schols D

更新日期：2002-09-11 00:00:00

abstract：:We investigated the expression of formyl peptide receptor (FPR) and its functional role in human bone marrow-derived mesenchymal stem cells (MSCs). We analyzed the expression of FPR by using ligand-binding assay with radio-labeled N-formyl-met-leu-phe (fMLF), and found that MSCs express FPR. FMLF stimulated intracellu...

journal_title：FEBS letters

authors： Kim MK,Min do S,Park YJ,Kim JH,Ryu SH,Bae YS

更新日期：2007-05-01 00:00:00

abstract：:A phylogenetic analysis, using the open reading frame I sequence of 93 TT viruses (TTV) obtained from various geographical areas, indicated that the virus could be classified into six different genotypes including three hitherto unreported genotypes. The high reliability of the six clusters was confirmed by bootstrap ...

journal_title：FEBS letters

authors： Tanaka Y,Mizokami M,Orito E,Ohno T,Nakano T,Kato T,Kato H,Mukaide M,Park YM,Kim BS,Ueda R

更新日期：1998-10-23 00:00:00

abstract：:The low density lipoprotein receptor-related protein (LRP) is a multifunctional cell surface receptor highly expressed in human aortic smooth muscle cells. In the present study, we used the short interfering RNA (siRNA) technique to explore the role of LRP in smooth muscle cell migration. We identified an LRP-specific...

journal_title：FEBS letters

authors： Li Y,Lu W,Bu G

更新日期：2003-12-04 00:00:00

abstract：:Crystals of methylamine-treated alpha 2-macroglobulin (alpha 2M-MA), alpha 2-macroglobulin in complex with two molecules of trypsin, alpha 2M-T2, one molecule of plasmin, alpha 2M-PL, and one molecule of plasmin followed by methylamine-treatment, alpha 2M-PL(MA), have reproducibly been obtained using ammonium sulfate ...

journal_title：FEBS letters

authors： Andersen GR,Jacobsen L,Thirup S,Nyborg J,Sottrup-Jensen L

更新日期：1991-11-04 00:00:00

abstract：:Measurements of the effects of NH3/NH4+ on glucose-induced electrical activity in beta-cells from microdissected mouse islets of Langerhans and on intracellular pH in single collagenase-isolated islets pre-loaded with a fluorescent pH probe were performed and are reported here. Application of NH3/NH4+ (15 mM) in the p...

journal_title：FEBS letters

authors： Rosario LM,Rojas E

更新日期：1986-05-05 00:00:00

abstract：:Small organic molecules, like Congo red and lacmoid, have been shown to modulate the self-assembly of the amyloid β peptide (Aβ). Here, we show that Aβ forms NMR invisible non-toxic co-aggregates together with lacmoid as well as Congo red. We find that the interaction involves two distinct kinetic processes and at eve...

journal_title：FEBS letters

authors： Abelein A,Lang L,Lendel C,Gräslund A,Danielsson J

更新日期：2012-11-16 00:00:00

abstract：:The level of reduction of cytochrome a and CuA during the oxidation of ferrocytochrome c has been determined in stopped-flow experiments. Both components are partially reduced but become progressively more oxidized as the reaction proceeds. When all cytochrome c has been oxidized, CuA is also completely oxidized, wher...

journal_title：FEBS letters

authors： Brzezinski P,Thörnström PE,Malmström BG

更新日期：1986-01-01 00:00:00

abstract：:Two fatty acid ethyl ester (FAEE) synthase isoenzymes purified from human myocardium were reported to be glutathione S-transferases (GST) [(1989) Proc. Natl. Acad. Sci. USA 86, 4470-4473; and (1989) J. Clin. Invest. 84, 1942-1946]. In the present study, the FAEE synthase activity of several purified and well character...

journal_title：FEBS letters

authors： Suzuki T,Kovacs MS,Board PG

更新日期：1990-11-26 00:00:00

abstract：:Iron-sulfur cluster biosynthesis depends on protein machineries, such as the ISC and SUF systems. The reaction is proposed to imply binding of sulfur and iron atoms and assembly of the cluster within a scaffold protein followed by transfer of the cluster to recipient apoproteins. The SufA protein from Escherichia coli...

journal_title：FEBS letters

authors： Sendra M,Ollagnier de Choudens S,Lascoux D,Sanakis Y,Fontecave M

更新日期：2007-04-03 00:00:00

abstract：:Malonyl-CoA-acyl carrier protein transacylase (MCAT) transfers the malonyl group from malonyl-CoA to holo-acyl carrier protein (ACP), and since malonyl-ACP is a key building block for fatty-acid biosynthesis it is considered as a promising antibacterial target. The crystal structures of MCAT from Staphylococcus aureus...

journal_title：FEBS letters

authors： Hong SK,Kim KH,Park JK,Jeong KW,Kim Y,Kim EE

更新日期：2010-03-19 00:00:00

abstract：:Serine/threonine phosphatases such as PP1, PP2A, and PP2B are well known to regulate the transition phase of the cell cycle. However, the function of PP2Cgamma in cell cycle progression is still unclear. In the present study, we report the characterization of PP2Cgamma in mammalian cells during the cell cycle. After r...

journal_title：FEBS letters

authors： Suh EJ,Kim TY,Kim SH

更新日期：2006-11-13 00:00:00

abstract：:A cell-free protein expression system was established that provides protein samples of adequate concentration and purity for direct NMR analysis. The Escherichia coli peptidyl-prolyl cis-trans isomerase PpiB was expressed in this system with dual amino acid-selective isotope labeling to identify the NMR signal from th...

journal_title：FEBS letters

authors： Guignard L,Ozawa K,Pursglove SE,Otting G,Dixon NE

更新日期：2002-07-31 00:00:00

abstract：:The yeast gene, ACP1, encoding the mitochondrial acyl carrier protein, was deleted by gene replacement. The resulting acp1-deficient mutants had only 5-10% of the wild-type lipoic acid content remaining, and exhibited a respiratory-deficient phenotype. Upon meiosis, the lipoate deficiency co-segregated with the acp1 d...

journal_title：FEBS letters

authors： Brody S,Oh C,Hoja U,Schweizer E

更新日期：1997-05-19 00:00:00

abstract：:A single chain ribosome-inactivating protein with RNA N-glycosidase activity, here named Dianthin 29, was isolated from leaves of Dianthus barbatus L. Incubation of intact Escherichia coli ribosomes with Dianthin 29 and subsequent aniline treatment of the isolated rRNA releases a rRNA fragment of 243 nucleotides from ...

journal_title：FEBS letters

authors： Prestle J,Hornung E,Schönfelder M,Mundry KW

更新日期：1992-02-10 00:00:00

abstract：:Analysis of the fate of the p185HER2 oncoprotein following activation by heregulin beta1 revealed the induction of the tyrosine-phosphorylation, down-modulation, and polyubiquitination of p185HER2. Receptor ubiquitination was suppressed in cells treated with heregulin beta1 in the presence of sodium azide, an inhibito...

journal_title：FEBS letters

authors： Magnifico A,Tagliabue E,Ardini E,Casalini P,Colnaghi MI,Ménard S

更新日期：1998-01-30 00:00:00

abstract：:In contrast to the preponderance of affected males in families with X-linked mental retardation, Rett syndrome (RTT) is a neurological disorder occurring almost exclusively in females. The near complete absence of affected males in RTT families has been explained by the lethal effect of an X-linked gene mutation in he...

journal_title：FEBS letters

authors： Orrico A,Lam C,Galli L,Dotti MT,Hayek G,Tong SF,Poon PM,Zappella M,Federico A,Sorrentino V

更新日期：2000-09-22 00:00:00

abstract：:Cyclic ADP-ribose (cADPR) is a natural metabolite of beta-NAD+ with a potent Ca2+-mobilizing activity in different cell types, including T-lymphocytes. We investigated (i) whether stimulation of T-lymphocytes with different agonists affects the intracellular concentration of cADPR, and (ii) whether the lymphocyte anti...

journal_title：FEBS letters

authors： da Silva CP,Schweitzer K,Heyer P,Malavasi F,Mayr GW,Guse AH

更新日期：1998-11-20 00:00:00

abstract：:The marked difference in auxin sensitivity between plant roots and shoots was studied in terms of auxin-induced stimulation of membrane P-type ATPase. The results suggest the existence in rice seedlings of, at least, two isoforms of soluble proteinous factors (SPF), SPF(I) and SPF(II), which are involved in the stimul...

journal_title：FEBS letters

authors： Kim D,Kim YS,Jung J

更新日期：1997-06-09 00:00:00

abstract：:Rats bearing the fast-growing AH-130 Yoshida ascites hepatoma showed a marked cachectic response which has been previously reported [Tessitore et al. (1987) Biochem. J. 241, 153-159]. Thus tumour-bearing animals showed significant decreases in body and muscle weight (soleus and gastrocnemius) as compared to both pair-...

journal_title：FEBS letters

authors： Llovera M,García-Martínez C,Agell N,Marzábal M,López-Soriano FJ,Argilés JM

更新日期：1994-02-07 00:00:00

abstract：:Calmodulin is shown to interact with human spectrin dimer. The binding was highly calcium-dependent and observed in two different kinds of experiments. Firstly, affinity chromatography of calmodulin on a Sepharose 4B column with immobilized spectrin, and secondly, partition in aqueous two-phase polymer systems. In the...

journal_title：FEBS letters

authors： Berglund A,Backman L,Shanbhag VP

更新日期：1984-06-25 00:00:00

abstract：:Jasmonic acid (JA) and its derivatives are well-characterized signaling molecules in plant defense and development, but the site of their localization within plant tissue is entirely unknown. To address the question whether applied JA accumulates extracellularly or intracellularly, leaves of tomato and barley were fed...

journal_title：FEBS letters

authors： Bücking H,Förster H,Stenzel I,Miersch O,Hause B

更新日期：2004-03-26 00:00:00

abstract：:Retinal dehydrogenase (RALDH) catalyzes the oxidation of retinal to all-trans and 9-cis retinoic acid, which function as ligands controlling RAR and RXR nuclear receptor-signaling pathways. We have recently shown the expression of RALDH transcript in the stomach and small intestine by reverse transcription polymerase ...

journal_title：FEBS letters

authors： Bhat PV

更新日期：1998-04-17 00:00:00

abstract：:Membrane proteins are usually solubilized in polar solvents by incorporation into micelles. Even for small membrane proteins these mixed micelles have rather large molecular masses, typically beyond 50000 Da. The NMR technique TROSY (transverse relaxation-optimized spectroscopy) has been developed for studies of struc...

journal_title：FEBS letters

authors： Fernández C,Hilty C,Bonjour S,Adeishvili K,Pervushin K,Wüthrich K

更新日期：2001-08-31 00:00:00

abstract：:Pyoverdine I (PVDI) is the major siderophore produced by Pseudomonas aeruginosa to import iron. Biosynthesis of this chelator involves non-ribosomal peptide synthetases and other enzymes. PvdQ is a periplasmic enzyme from the NTN hydrolase family and is involved in the final steps of PVDI biosynthesis. A pvdQ mutant p...

journal_title：FEBS letters

authors： Hannauer M,Schäfer M,Hoegy F,Gizzi P,Wehrung P,Mislin GL,Budzikiewicz H,Schalk IJ

更新日期：2012-01-02 00:00:00

abstract：:It is demonstrated by filter-binding assay that casein kinase 2 from Rana temporaria oocytes binds rRNA in vitro with high affinity. Ligand-blotting shows that rRNA-binding activity is inherent to alpha and alpha' subunits of the enzyme. Increase of pH from 6.5 to 7.5 has little effect on casein kinase but completely ...

journal_title：FEBS letters

authors： Kandror KV,Kapkov DV,Turapov OA,Stepanov AS

更新日期：1991-06-03 00:00:00