Abstract:
:Retinal dehydrogenase (RALDH) catalyzes the oxidation of retinal to all-trans and 9-cis retinoic acid, which function as ligands controlling RAR and RXR nuclear receptor-signaling pathways. We have recently shown the expression of RALDH transcript in the stomach and small intestine by reverse transcription polymerase chain reaction [Bhat, P.V., Labrecque J., Dumas, F., Lacroix, A. and Yoshida, A. (1995) Gene 166, 303-306]. We have examined RALDH expression in the stomach and small intestine before and during postnatal development and in vitamin A deficiency by assaying for mRNA levels and protein as well as for enzyme activity. In -2 day fetuses, RALDH expression was high in the small intestine, whereas RALDH protein was not detectable in the stomach. However, expression of RALDH was seen in the stomach after birth, and gradually increased with age and reached the highest level at postnatal day 42. In the intestine, RALDH expression decreased postnatally. Vitamin A deficiency up-regulated RALDH expression in the stomach and small intestine, and administration of retinoids down-regulated the RALDH expression in these tissues. These results show the differential expression of RALDH in the stomach and small intestine during postnatal development, and that vitamin A status regulates the expression of RALDH gene in these tissues.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Bhat PVdoi
10.1016/s0014-5793(98)00355-xsubject
Has Abstractpub_date
1998-04-17 00:00:00pages
260-2issue
2eissn
0014-5793issn
1873-3468pii
S0014-5793(98)00355-Xjournal_volume
426pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The bacteriophytochrome Agp1 was reconstituted with a locked 5Zs-biliverdin in which the C(4)=C(5) and C(5)-C(6) bonds of the methine bridge between rings A and B are fixed in the Z and syn configuration/conformation, respectively. In Agp1-5Zs the photoconversion proceeds via the Lumi-R intermediate to Meta-R(A), but ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.10.043
更新日期:2007-11-27 00:00:00
abstract::Contribution of sphingosine kinase (SPK)-catalyzed production of sphingosine-1-phosphate (SPP), in comparison to phospholipase C (PLC), to Ca(2+) signalling by epidermal growth factor (EGF) was studied in two HEK-293 cell clones (HEK2 and HEK3), expressing functional EGF receptors and exhibiting release of stored Ca(2...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01463-5
更新日期:1999-11-19 00:00:00
abstract::We have characterized somatostatinergic phenotype markers of the human neuroblastoma, LA-N-2. A single mRNA-transcript (approximately 850bp) and two cellular somatostatin immunoreactivity forms, a high molecular weight form (M(r) 15,000) and a fragment corresponding to somatostatin-28 was found, while the somatostatin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00958-c
更新日期:1995-09-18 00:00:00
abstract::Recently, we have reported the presence of kinetin (N6-furfuryladenine) in commercially available DNA, in freshly extracted cellular DNA and in plant cell extracts. We have also found that kinetin has electrochemical properties which can be used for monitoring the level of this modified base in DNA. Here, for the firs...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01037-5
更新日期:1997-09-08 00:00:00
abstract::Living isolated mitochondria incubated with iron(II) show a major alteration in mitochondrial DNA (mtDNA) conformational forms as assessed by Southern blot analysis of undigested mtDNA. In the presence of iron(II), form I is transformed into form III in a dose-dependent manner. This alteration in mtDNA conformation sh...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01768-3
更新日期:2000-09-01 00:00:00
abstract::The cofactor of several flavoenzymes is autocatalytically bound to the polypeptide via a histidyl(N3)-(8alpha)-FAD linkage which makes the generation of apoenzyme difficult. We introduced an alternative covalent protein-FAD bond at the active site of 6-hydroxy-D-nicotine oxidase (6HDNO) by replacing the FAD-binding hi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00438-3
更新日期:1996-05-20 00:00:00
abstract::Multidrug transporters are involved in mediating the failure of chemotherapy in treating several serious diseases. The archetypal multidrug transporter P-glycoprotein (P-gp) confers resistance to a large number of chemically and functionally unrelated anti-cancer drugs by mediating efflux from cancer cells. The abilit...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2005.11.083
更新日期:2006-02-13 00:00:00
abstract::The early response gene IEX-1 modulates apoptosis and cell growth in a poorly defined fashion. Here, we describe the effect of hammerhead ribozymes specifically disrupting IEX-1 expression in 293 cells. Compared to vector control, 293 cells exhibit a reduced growth rate and a slowed cell cycle progression, when stably...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02344-4
更新日期:2001-04-13 00:00:00
abstract::The effect of activation of endogenous c-Src tyrosine kinase by (pY)EEI peptide was examined on voltage-operated calcium channel (VOC) currents in arterial smooth muscle cells. In single rabbit ear artery cells intracellular application of (pY)EEI peptide increased calcium channel currents. Inactive, non-phosphorylate...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01177-5
更新日期:1996-12-09 00:00:00
abstract::The effect of expression of c-fos gene on proteoglycan synthesis, one of the important markers of cartilage metabolism, was examined by introducing the c-fos DNA into HCS 2/8 chondrocytes. The [35S]sulfate incorporation into proteoglycan was decreased in the c-fos transfectants expressing exogenous c-fos mRNA, when co...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00118-4
更新日期:1996-03-04 00:00:00
abstract::1-Farnesylpyridinium (FPy), an analog of isoprenoid farnesol, initially induced morphological changes similar to those of typical apoptosis in human leukemia HL-60 cells but FPy-treated cells were characterized by the absolute absence of final apoptotic events such as fragmentation into apoptotic bodies. FPy-induced c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02373-6
更新日期:2002-03-13 00:00:00
abstract::The interaction of Bordetella pertussis adenylate cyclase toxin (CyaA) with complement receptor 3 (CR3, CD11b/CD18) involves N-linked oligosaccharide chains. To investigate the relative importance of the individual N-glycans of CR3 for toxin activity, the asparagine residues of the consensus N-glycosylation sites of C...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.12.023
更新日期:2015-01-30 00:00:00
abstract::The contents of sterol derivatives (sterols + esters, glycosides and acylated glycosides) have been determined using thin layer chromatography in leaves and bulky and organs from carrots (Daucus carota L.) potato (Solanum tuberosum L.), onion (Allium cepa L.) two varieties of radish (Raphanus sativus L.) and Gentiana ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(70)80522-1
更新日期:1970-12-01 00:00:00
abstract::The effect of platelet-activating factor (PAF) on polyphosphoinositide metabolism and 45Ca2+ efflux was examined in a vascular smooth muscle cell line (A7r5). PAF stimulated a rapid but transient production of inositol trisphosphate and inositol bisphosphate which, in the presence of lithium, resulted in an accumulati...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80288-5
更新日期:1986-03-03 00:00:00
abstract::Rat brain S-100 (S-100b) and ox brain S-100a0, S-100a and S-100b have been tested for their ability to control the assembly and disassembly of brain microtubule proteins in the presence of either Ca2+ or Zn2+, in vitro. In the presence of Ca2+, single S-100 isoforms have similar, if not identical, effects, i.e. they i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81340-5
更新日期:1985-07-01 00:00:00
abstract::Cytosolic Ca2+ concentration and membrane potential were monitored in individual cultured endothelial cells mechanically stimulated with a micropipette attached to the stage of a microscope. Both dimpling and poking of endothelial cells resulted in Ca2+i transients (from 63 +/- 12 to 397 +/- 52 nM, characterized by a ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80340-5
更新日期:1988-11-21 00:00:00
abstract::Drosophila PTEN (dPTEN) plays indispensable roles in the development of Drosophila melanogaster by controlling cell size and number. Although three potential spliced forms of dPTEN have been isolated, functional distinction among these forms remains elusive. In this study, we demonstrate that all spliced forms of dPTE...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.03.074
更新日期:2004-05-07 00:00:00
abstract::A simple method for isolation and fluorescence labelling of Leydig cells (L-cells) from rat testes was developed. Lateral diffusion coefficients of both lipid and protein membrane fluorescent probes were measured by the method of fluorescence recovery after photobleaching (FRAP). Age-dependent changes in diffusibility...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80189-8
更新日期:1987-09-28 00:00:00
abstract::Tomato Cf-9, a receptor-like protein (RLP), confers resistance to races of the fungal pathogen Cladosporium fulvum that express the Avr9 avirulence gene. CITRX (Cf-9-interacting thioredoxin) was previously identified in a yeast two-hybrid screen as a protein interacting with the cytoplasmic domain of Cf-9 and shown to...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.06.077
更新日期:2006-07-24 00:00:00
abstract::The early embryonic development of Xenopus is mainly governed by post-transcriptional regulations until the mid-blastula transition. In this report, we present evidence demonstrating that fertilization of Xenopus eggs triggers a complete translational arrest of mRNAs containing UA-rich elements in their 3'-untranslate...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00413-7
更新日期:1994-05-30 00:00:00
abstract::Fibroblast growth factors (FGFs) and their receptors are expressed in a variety of mammalian tissues, playing a role in development and cell proliferation. While analyzing human sperm motility, we found that sperm treated with endo-β-galactosidase (EBG), which specifically hydrolyzes poly-N-acetyllactosamine type glyc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.07.056
更新日期:2013-10-01 00:00:00
abstract::The solution conformation of the cardiac stimulatory sea anemone polypeptide anthopleurin-A has been characterised using distance geometry and restrained molecular dynamics calculations. A set of 253 approximate interproton distance restraints and 14 peptide backbone torsion angle restraints derived from two-dimension...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80931-1
更新日期:1988-11-07 00:00:00
abstract::We performed microarray and computational gene network analyses to identify the detailed mechanisms by which sodium butyrate (SB) induces cell growth arrest and the differentiation of mouse colonic epithelial MCE301 cells. Two thousand six hundred four differentially expressed probe sets were identified in the cells t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.04.048
更新日期:2006-05-29 00:00:00
abstract::Maculatin 1.1 is an antimicrobial peptide isolated from the Australian tree frog Litoria genimaculata that adopts an amphipathic, alpha-helical structure in solution. Its orientation and conformation when incorporated to pre-formed DMPG (1,2-dimyristoyl-sn-glycero-3-phosphoglycerol) and DMPC (1,2-dimyristoyl-sn-glycer...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03313-0
更新日期:2002-02-13 00:00:00
abstract::A-disintegrin and metalloproteinase 10 (ADAM10) is involved in the generation of amyloid-β (Aβ) during amyloid precursor protein (APP) processing, and has a protective effect against Aβ neurotoxicity. We explored how metallothionein-III (MT-III) is regulated in the non-amyloidogenic pathway to generate soluble APPα (s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.05.017
更新日期:2014-06-27 00:00:00
abstract::The organization of the U1snRNP-specific A protein (34 kDa) has been analyzed by 12 and 16 A thiol-reversible chemical cross-linking and Western blotting. A-containing cross-linked complexes had molecular masses of 43, 47, 56, 62, 67, 105 and 125 kDa. None of these complexes could be cross-linked following ribonucleas...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80487-4
更新日期:1990-10-15 00:00:00
abstract::Macrolides are a group of antibiotics structurally characterized by a macrocyclic lactone to which one or several deoxy-sugar moieties are attached. The sugar moieties are transferred to the different aglycones by glycosyltransferases (GTF). The OleI GTF of an oleandomycin producer, Streptomyces antibioticus, catalyze...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01721-x
更新日期:2000-07-07 00:00:00
abstract::Two hybrid proteins between Pseudomonas aeruginosa exotoxin A (PE) and poliovirus protease 2Apro have been generated. One hybrid protein contains the poliovirus 2Apro sequence replacing the region of PE corresponding to amino acids 413-607. The other hybrid contains in addition the transforming growth factor sequence....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01157-5
更新日期:1994-11-21 00:00:00
abstract::We have previously shown that the Mycoplasma mycoides glycine tRNA (anticodon UCC) effectively reads the codons GGU and GGC in violation of the classic codon reading rules. We have attempted to elucidate what structural elements in this tRNA molecule confer this translational property and in the course of this investi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81077-2
更新日期:1990-10-29 00:00:00
abstract::Many biochemical reactions are confined to interfaces, such as membranes or cell walls. Despite their importance, no canonical rate laws describing the kinetics of surface-active enzymes exist. Combining the approach chosen by Michaelis and Menten 100 years ago with concepts from surface chemical physics, we here pres...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.07.026
更新日期:2013-09-02 00:00:00