Abstract:
:The early response gene IEX-1 modulates apoptosis and cell growth in a poorly defined fashion. Here, we describe the effect of hammerhead ribozymes specifically disrupting IEX-1 expression in 293 cells. Compared to vector control, 293 cells exhibit a reduced growth rate and a slowed cell cycle progression, when stably transfected with a concatemeric ribozyme construct. In addition, these 293 cells were much less sensitive to apoptosis induced by an activating Fas/CD95 antibody or by the anti-cancer drugs etoposide and doxorubicin. By modulating the cell cycle, IEX-1 might be part of a growth signal if favourable growth conditions prevail, whereas under unfavourable conditions, i.e. death receptor activation, IEX-1 facilitates apoptosis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Grobe O,Arlt A,Ungefroren H,Krupp G,Fölsch UR,Schmidt WE,Schäfer Hdoi
10.1016/s0014-5793(01)02344-4keywords:
subject
Has Abstractpub_date
2001-04-13 00:00:00pages
196-200issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(01)02344-4journal_volume
494pub_type
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