Abstract:
:The aim of the present study is to determine the role of intracellular Ca(2+) in VEGF signaling. We demonstrate that reduction in Ca(2+) by chelating compound BAPTA-AM or by IP(3)-endoplasmic reticulum blocker 2-APB selectively inhibited VEGF-induced activation of c-Src-PI3K-Akt but not ERK1/2 in human coronary artery endothelial cells (HCAEC). We also show that the selective inhibitory effects of NADPH oxidase knockdown on VEGF-mediated activation of c-Src-PI3K-Akt signaling and cell proliferation in HCAEC can be reversed by increase in intracellular Ca(2+). These results suggest an essential role for Ca(2+) in redox-dependent selective activation of c-Src-PI3K-Akt and endothelial cell proliferation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Lee M,Spokes KC,Aird WC,Abid MRdoi
10.1016/j.febslet.2010.05.053subject
Has Abstractpub_date
2010-07-16 00:00:00pages
3131-6issue
14eissn
0014-5793issn
1873-3468pii
S0014-5793(10)00457-6journal_volume
584pub_type
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