Macromolecular crowding increases structural content of folded proteins.

abstract：:When bottromycin A2 was added to an in vitro protein synthesis system carried out by naturally occurring polysomes, it inhibited protein synthesis effectively. Examination of the 3 steps of peptide chain elongation revealed that the binding of aminoacyl-tRNA to the polyribosomes was inhibited by bottromycin A2. In con...

journal_title：FEBS letters

authors： Otaka T,Kaji A

更新日期：1983-03-07 00:00:00

abstract：:The odorant-induced accumulation of cAMP can be inhibited by antibodies directed against G alpha s/olf. In contrast, antibodies raised against G alpha i-subunits caused a strong enhancement of the odorant-induced cAMP accumulation. Western blotting and immunoelectron microscopy revealed the presence of both G alpha s/...

journal_title：FEBS letters

authors： Sinnarajah S,Ezeh PI,Pathirana S,Moss AG,Morrison EE,Vodyanoy V

更新日期：1998-04-24 00:00:00

abstract：:The expression of interphotoreceptor retinoid binding protein (IRBP) is limited to photoreceptor cells of the retina and pinealocytes of the pineal gland. We sought to define cis-elements of the mouse IRBP 5' flanking region that are required for this restricted activity. In vitro transient transfections of retinoblas...

journal_title：FEBS letters

authors： Boatright JH,Knox BE,Jones KM,Stodulkova E,Nguyen HT,Padove SA,Borst DE,Nickerson JM

更新日期：2001-08-24 00:00:00

abstract：:We have reported previously on the ability of Lactobacillus reuteri to produce a compound with vitamin B(12) activity. Here we report on the chemical characterisation of this corrinoid-like molecule. High performance liquid chromatography coupled to an ultraviolet diode array detector, mass spectrometry and nuclear ma...

journal_title：FEBS letters

authors： Santos F,Vera JL,Lamosa P,de Valdez GF,de Vos WM,Santos H,Sesma F,Hugenholtz J

更新日期：2007-10-16 00:00:00

abstract：:Recently, it has been shown that really interesting new gene (RING)-in between ring finger (IBR)-RING domain-containing proteins, such as Parkin and Parc, are E3 ubiquitin ligases and are involved in regulation of apoptosis. In this report, we show that p53-inducible RING-finger protein (p53RFP), a p53-inducible E3 ub...

journal_title：FEBS letters

authors： Huang J,Xu LG,Liu T,Zhai Z,Shu HB

更新日期：2006-02-06 00:00:00

abstract：:During steady-state, the Pi released in the medium is derived from glucose-6-phosphate which continuously regenerates the ATP hydrolyzed. A membrane potential (delta psi) can be built up in submitochondrial particles using glucose-6-phosphate and hexokinase as an ATP-regenerating system. The energy derived from the me...

journal_title：FEBS letters

authors： de Meis L,Grieco MA,Galina A

更新日期：1992-08-17 00:00:00

abstract：:In Alzheimer's disease (AD) brain, microtubule-associated protein tau is abnormally modified by hyperphosphorylation and glycosylation, and is aggregated as neurofibrillary tangles of paired helical filaments. To investigate the role of tau glycosylation in neurofibrillary pathology, we isolated various pools of tau p...

journal_title：FEBS letters

authors： Liu F,Zaidi T,Iqbal K,Grundke-Iqbal I,Merkle RK,Gong CX

更新日期：2002-02-13 00:00:00

abstract：:We have studied the modulation and pharmacological properties of two anion channels in T84 cells by recording single channel and transepithelial currents. One channel had an outwardly rectifying current-voltage I/V curve, was rarely active in cell-attached patches, and was unaffected by cAMP. The other channel had low...

journal_title：FEBS letters

authors： Tabcharani JA,Low W,Elie D,Hanrahan JW

更新日期：1990-09-17 00:00:00

abstract：:Evolutionary trees based on partial small ribosomal subunit RNA sequences of 22 metazoa species have been published [(1988) Science 239, 748-753]. In these trees, cnidarians (Radiata) seemed to have evolved independently from the Bilateria, which is in contradiction with the general evolutionary view. In order to furt...

journal_title：FEBS letters

authors： Hendriks L,Van de Peer Y,Van Herck M,Neefs JM,De Wachter R

更新日期：1990-09-03 00:00:00

abstract：:Three mutant polypeptides of the type I 37-residue winter flounder 'antifreeze' protein have been synthesized. All four threonine residues in the native peptide were been mutated to serine, valine and glycine respectively and two additional salt bridges were incorporated into the sequences in order to improve aqueous ...

journal_title：FEBS letters

authors： Haymet AD,Ward LG,Harding MM,Knight CA

更新日期：1998-07-03 00:00:00

abstract：:WT1 encodes a zinc finger protein with a key role in urogenital development that is inactivated in a subset of Wilms' tumors. This tumor suppressor gene product contains an amino-terminal dimerization domain required for trans-inhibition of wild-type WT1 activity by mutants defective for DNA binding. In the course of ...

journal_title：FEBS letters

authors： Bruening W,Moffett P,Chia S,Heinrich G,Pelletier J

更新日期：1996-09-09 00:00:00

abstract：:Antimycin, 2-nonyl-4-hydroxyquinoline N-oxide and funiculosin induce O.2(-) generation by submitochondrial particles oxidizing succinate, whereas KCN, mucidin, myxothiazol or 2,3-dimercaptopropanol inhibit O.2(-) generation. Thenoyltrifluoroacetone does not induce superoxide production by itself but slightly stimulate...

journal_title：FEBS letters

authors： Ksenzenko M,Konstantinov AA,Khomutov GB,Tikhonov AN,Ruuge EK

更新日期：1983-05-02 00:00:00

abstract：:CREB binding protein (CBP) is an acetyltransferase that plays an important role in many biological processes. Here, we show that Akt phosphorylates CBP at threonine 1871 and suppresses its acetyltransferase activity by impeding the binding of CBP to histone H3, which results in a decrease in lysine K18 acetylation and...

journal_title：FEBS letters

authors： Liu Y,Xing ZB,Zhang JH,Fang Y

更新日期：2013-04-02 00:00:00

abstract：:Cytoplasmic colicin A has the ability to bind to membranes and to form stable dimers. This form remains stable even in the presence of 1% SDS at 25 degrees C. Both of these properties were not observed for extracellular colicin A suggesting a possible difference in the conformation between cytoplasmic and extracellula...

journal_title：FEBS letters

authors： Knibiehler M,Lazdunski C

更新日期：1987-06-01 00:00:00

abstract：:The photocycle of the light-adapted purple membrane was studied with a time-resolved fluorometry apparatus: fluorescence of the sample suspension (greater than 660 nm) was pumped with a 633-nm cw laser and the temporal change induced by a 532-nm pulsed laser was measured with a photon-counting-type transient recorder....

journal_title：FEBS letters

authors： Ohtani H,Itoh H,Shinmura T

更新日期：1992-06-22 00:00:00

abstract：:The incorporation of carbon into the carbon monoxide and cyanide ligands of [NiFe]-hydrogenases has been investigated by using (13)C labelling in infrared studies of the Allochromatium vinosum enzyme and by (14)C labelling experiments with overproduced Hyp proteins from Escherichia coli. The results suggest that the b...

journal_title：FEBS letters

authors： Roseboom W,Blokesch M,Böck A,Albracht SP

更新日期：2005-01-17 00:00:00

abstract：:JAK2 V617F mutant induces transformation through aberrant activation of various transcription factors. We found that the expression of Aurora kinase A (Aurka) was significantly induced by mutant JAK2 through c-Myc expression. Interestingly, mutant JAK2 enhanced resistance to cisplatin (CDDP)-induced DNA damage, and ef...

journal_title：FEBS letters

authors： Sumi K,Tago K,Kasahara T,Funakoshi-Tago M

更新日期：2011-06-23 00:00:00

abstract：:Computer modelling with an energy minimization procedure is used here to obtain stereochemical and energetic details for complexes of the dye Hoechst 33258 with different oligonucleotide sequences. An optimised model of the dye with d(A)5 X d(T)5 is in conformity with previous proposed models. It has bifurcated hydrog...

journal_title：FEBS letters

authors： Gopalakrishna K,Kalia A,Royyuru AK,Mrigank,Atreyi M,Rao MV,Kothekar V

更新日期：1987-05-04 00:00:00

abstract：:Msn2 and Msn4 transcription factors activate expression of stress-responsive element (STRE) controlled genes in response to various stresses triggering the environmental stress response in Saccharomyces cerevisiae. Although high hydrostatic pressure is known to induce gene expression modification in yeast, the transcr...

journal_title：FEBS letters

authors： Domitrovic T,Fernandes CM,Boy-Marcotte E,Kurtenbach E

更新日期：2006-11-13 00:00:00

abstract：:Antifungal activity has been associated with 2 immunochemically distinct proteins, protein R and S (Mr approximately 23 kDa; pI 9-10), which were isolated in pure form from barley grain. The proteins are homologous with thaumatin- and pathogenesis-related proteins of the PR5 family. The proteins inhibit growth of i.a....

journal_title：FEBS letters

authors： Hejgaard J,Jacobsen S,Svendsen I

更新日期：1991-10-07 00:00:00

abstract：:Oxaloacetate decarboxylase of Klebsiella pneumoniae was shown to contain between 0.6 and 1.0 mol zinc per mol enzyme in different preparations. The decarboxylase activity was completely abolished after 15 min incubation with 1 mM Hg(NO3)2 in phosphate buffer, while the activity decreased only 20% if the incubation was...

journal_title：FEBS letters

authors： Dimroth P,Thomer A

更新日期：1992-03-23 00:00:00

abstract：:The lipid A structure of the Gram-negative bacterium Helicobacter mustelae, a ferret gastric pathogen responsible for the onset of gastric diseases in its host, was investigated. Two variant lipid A structures were found in the same strain. One structure contained a bisphosphorylated beta-(1-->6)-linked D-glucosamine ...

journal_title：FEBS letters

authors： Thérisod H,Monteiro MA,Perry MB,Caroff M

更新日期：2001-06-15 00:00:00

abstract：:Over 400 human proteases documented in secondary databases can already be delineated in genomic sequence. A Genome Ontology annotation of 30585 sequences in the provisional human proteome set recognises 498 proteases, i.e. 1.6%. Homology searches against finished sequence and comparisons between mouse and zebrafish ar...

journal_title：FEBS letters

authors： Southan C

更新日期：2001-06-08 00:00:00

abstract：:A modular microscope-based screening platform, with applications in large-scale analysis of protein function in intact cells is described. It includes automated sample preparation, image acquisition, data management and analysis, and the genome-wide automated retrieval of bioinformatic information. The modular nature ...

journal_title：FEBS letters

authors： Liebel U,Starkuviene V,Erfle H,Simpson JC,Poustka A,Wiemann S,Pepperkok R

更新日期：2003-11-20 00:00:00

abstract：:A new epsilon-N-trimethyllysine metabolite has been isolated from the mycelium of Neurospora crassa. The labelled compound produced from incubations in vivo and in vitro from epsilon-N-trimethyl ([14-C]H3)L-lysine has been identified as 2-keto-epsilon-N-trimethyl-hexanoic acid by reducing its 2,4-dinitrophenyl hydrazo...

journal_title：FEBS letters

authors： Villanueva VR,Lederer E

更新日期：1975-04-01 00:00:00

abstract：:The present study discusses the efficacy of Aloe emodin-8-O-glycoside (AEG), a plant derived anthroquinone, on alleviating insulin resistance and augmenting glycogen synthesis in L6 myotubes and 3T3L1 adipocytes. Dose-dependent increase in glucose uptake activity (GUA) was observed in both cell lines. Immunoblot analy...

journal_title：FEBS letters

authors： Anand S,Muthusamy VS,Sujatha S,Sangeetha KN,Bharathi Raja R,Sudhagar S,Poornima Devi N,Lakshmi BS

更新日期：2010-07-16 00:00:00

abstract：:The C1 domains are 50 amino acid sequences present in protein kinase C (PKC) isozymes that are responsible for binding of phorbol esters and the lipid second messenger diacylglycerol (DAG). We found that bacterially expressed C1b domain of PKCdelta induces germinal vesicle breakdown (GVBD) when microinjected into Xeno...

journal_title：FEBS letters

authors： Aroca P,Santos E,Kazanietz MG

更新日期：2000-10-13 00:00:00

abstract：:Cyanobacteria possess genes encoding phytochrome-related proteins. We used a DNA microarray approach to evaluate the impact of the phytochromes Cph1 and Cph2 on red light (R)- and far-red light (FR)-dependent gene expression in the unicellular cyanobacterium Synechocystis sp. PCC 6803. In cells of wild-type and phytoc...

journal_title：FEBS letters

authors： Hübschmann T,Yamamoto H,Gieler T,Murata N,Börner T

更新日期：2005-03-14 00:00:00

abstract：:The organization of the U1snRNP-specific A protein (34 kDa) has been analyzed by 12 and 16 A thiol-reversible chemical cross-linking and Western blotting. A-containing cross-linked complexes had molecular masses of 43, 47, 56, 62, 67, 105 and 125 kDa. None of these complexes could be cross-linked following ribonucleas...

journal_title：FEBS letters

authors： Boix J,Habets WJ,van Venrooij WJ,Smith HC

更新日期：1990-10-15 00:00:00

abstract：:Adenosine and ATP modulate cellular and tissue functions via specific P1 and P2 receptors, respectively. Although, in general, adenosine inhibits excitability and ATP functions as an excitatory transmitter in the central nervous system, little is known about the direct interaction between P1 and P2 receptors. We recen...

journal_title：FEBS letters

authors： Yoshioka K,Hosoda R,Kuroda Y,Nakata H

更新日期：2002-11-06 00:00:00