Abstract:
:A modular microscope-based screening platform, with applications in large-scale analysis of protein function in intact cells is described. It includes automated sample preparation, image acquisition, data management and analysis, and the genome-wide automated retrieval of bioinformatic information. The modular nature of the system ensures that it is rapidly adaptable to new biological questions or sets of proteins. Two automated functional assays addressing protein secretion and the integrity of the Golgi complex were developed and tested. This shows the potential of the system in large-scale, cell-based functional proteomic projects.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Liebel U,Starkuviene V,Erfle H,Simpson JC,Poustka A,Wiemann S,Pepperkok Rdoi
10.1016/s0014-5793(03)01197-9keywords:
subject
Has Abstractpub_date
2003-11-20 00:00:00pages
394-8issue
3eissn
0014-5793issn
1873-3468pii
S0014579303011979journal_volume
554pub_type
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