Abstract:
:The small GTPase Rho1 plays an essential role in controlling the organization of the actin cytoskeleton and synthesis of the cell wall in the fission yeast Schizosaccharomyces pombe. Here we studied the role of Rho5 whose primary structure is very similar to that of Rho1. It was found that elevated expression of Rho5 was able to compensate for the lethality of cells lacking Rho1. Rho5 was localized to the ends of interphase cells and the mid-region of mitotic cells. Overexpression of Rho5 caused depolarization of F-actin patches and abnormal formation of the cell wall, as did Rho1. Although rho5(+) was not essential for maintaining the cell shape, rho1 rho5-double null cells showed more severe defects in cell viability than rho1-null cells. Thus, it is likely that Rho5 has an overlapping function with Rho1 in controlling cell growth and division in S. pombe.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Nakano K,Arai R,Mabuchi Idoi
10.1016/j.febslet.2005.08.031keywords:
subject
Has Abstractpub_date
2005-09-26 00:00:00pages
5181-6issue
23eissn
0014-5793issn
1873-3468pii
S0014-5793(05)01026-4journal_volume
579pub_type
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