Abstract:
:Six chimeras of the skeletal muscle (RyR1) and cardiac muscle (RyR2) Ca(2+) release channels (ryanodine receptors) previously used to identify RyR1 dihydropyridine receptor interactions [Nakai et al. (1998) J. Biol. Chem. 273, 13403] were expressed in HEK293 cells to assess their Ca(2+) dependence in [(3)H]ryanodine binding and single channel measurements. The results indicate that the C-terminal one-fourth has a major role in Ca(2+) activation and inactivation of RyR1. Further, our results show that replacement of RyR1 regions with corresponding RyR2 regions can result in loss and/or reduction of [(3)H]ryanodine binding affinity while maintaining channel activity.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Nakai J,Gao L,Xu L,Xin C,Pasek DA,Meissner Gdoi
10.1016/s0014-5793(99)01232-6keywords:
subject
Has Abstractpub_date
1999-10-08 00:00:00pages
154-8issue
2eissn
0014-5793issn
1873-3468pii
S0014-5793(99)01232-6journal_volume
459pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::We quantitatively measured protective antigen (PA) binding to human cells targeted by anthrax lethal toxin (LT). Affinities were less than 50 nM for all cells, but differentiated cells (macrophages and neutrophils) had significantly increased PA binding and endothelial cells demonstrated the most binding. Combined wit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.06.070
更新日期:2006-07-24 00:00:00
abstract::A cDNA coding for the mouse hepatic triglyceride lipase has been isolated from a mouse liver cDNA library. The nucleotide sequence of the cDNA shows an open reading frame encoding a polypeptide of 510 amino acids that is 91.5% and 86% homologous to rat and human hepatic lipase, respectively. The most drastic protein s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80910-u
更新日期:1991-09-02 00:00:00
abstract::Bcl-xL, a close homolog of Bcl2, is an important regulator of apoptosis and is overexpressed in human cancer. Phosphorylation of Bcl-xL can be induced by microtubule-damaging drugs such as taxol or 2-methoxyestradiol (2-ME). By site-directed mutagenesis studies, we have identified that serine 62 is the necessary site ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00131-5
更新日期:2003-03-13 00:00:00
abstract::Six charged amino acid residues located in the ectodomain of the full-length type I transforming growth factor (TGF)-beta receptor were individually mutated to alanine. Mutation of residues D47, D98, K102 and E104 resulted in functionally impaired receptors as demonstrated by a marked decrease in ligand-dependent sign...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03231-8
更新日期:2002-02-27 00:00:00
abstract::In photosystem II (PSII) the probability that energy absorbed by core antenna chlorophyll (Chl) is transferred to the reaction center (RC) is extremely high. Although close proximity between antenna Chl ensures a high transfer efficiency, relative pigment orientation can fractionally modify it. This level of refinemen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/S0014-5793(04)00133-4
更新日期:2004-03-12 00:00:00
abstract::It is well known that, in rabbits, the allotypes of the b locus carried by the light chains of the IgG molecules cross-react. Nothing similar has been described for the allotypes of the a locus carried by the heavy chains of the IgG molecules. This paper describes a cross-reaction between the rabbit allotypic markers ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(69)80028-1
更新日期:1969-02-01 00:00:00
abstract::Extracellular regulated kinases (ERKs) mediate the inhibitory effect of tumor necrosis factor alpha (TNF-alpha) on uncoupling protein-1 (UCP-1), but not on lipid accumulation. TNF-alpha-induced ERK-dependent peroxisome proliferator activator receptor gamma (PPAR gamma) phosphorylation could be responsible for UCP-1 do...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02762-x
更新日期:2002-06-05 00:00:00
abstract::A series of 9-beta-D-xylofuranosyladenine (xyloA or xyloadenosine) substituted analogs of 2-5A core trimer and tetramer were examined for their ability to be degraded by the 2',5'-phosphodiesterase activity of cytoplasmic extracts of mouse L cells. Two distinct groups of xyloA-substituted analogs could be readily disc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81358-3
更新日期:1987-02-23 00:00:00
abstract::The eukaryotic transcription factor NF-kappaB is activated by a large variety of stimuli. We have recently shown that ER stress, caused by an aberrant accumulation of membrane proteins within this organelle, also activates NF-kappaB. Here, we show that activation of NF-kappaB by ER stress requires an increase in the i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00800-9
更新日期:1996-08-26 00:00:00
abstract::The secretion mechanisms of cathepsin L from osteoclasts in the process of bone resorption were investigated. The increases in bone pit numbers formed take place by PTH addition in parallel with the increases of cathepsin L and/or L-like proteinase activities in the culture medium of bone cells, and these were suppres...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80522-9
更新日期:1994-04-11 00:00:00
abstract::Endothelin-1 (ET-1) dose-dependently increased HIF1α expression in pulmonary artery smooth muscle cells (PASMCs). Inhibition of protein synthesis did not affect ET-1-induced HIF1α expression. The maximum effect of ET-1 was similar to that caused by proteasome inhibitor MG132. Further study indicates that ET-1 also dos...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.08.036
更新日期:2012-11-02 00:00:00
abstract::In rat cerebrocortical synaptosomes, the addition of 4 beta-phorbol dibutyrate (4 beta-PDBu) and arachidonic acid enhances and decreases, respectively, the glutamate release evoked by 4-aminopyridine. Pretreatment of synaptosomes with 12-O-tetradecanoylphorbol 13-acetate (TPA) or pre-incubation with staurosporine, pre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80312-5
更新日期:1992-01-27 00:00:00
abstract::The glycogen branching enzyme from Vibrio vulnificus (VvGBE) transfers short side chains (DP 3-5) significantly greater than any other bacterial glycogen branching enzyme (GBE). To elucidate the role of the N-domain of VvGBE in the unique branching pattern, domain-truncated (N1 and N) and N1-domain-swapped (with VvGBE...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.03.011
更新日期:2015-04-28 00:00:00
abstract::The effect of oligosaccharide carrying lipids on membrane fluidity has been investigated. Gangliosides GM1 and GQ1 were reconstituted into phosphatidylcholine bilayer membranes at low concentrations (less than 5 mol%). A strong fluidizing effect was observed leading to a suppression of the phase transition temperature...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80214-3
更新日期:1985-01-01 00:00:00
abstract::AtCYP20-2 is one of 16 immunophilins in thylakoid lumen. The presence of the isomerase domain in AtCYP20-2, an enrichment of AtCYP20-2 in the stroma membranes and it's co-migration with NAD(P)H dehydrogenase (NDH) in native gels provide evidence that AtCYP20-2 is an auxiliary protein of NDH. When different NDH mutants...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.06.031
更新日期:2009-07-21 00:00:00
abstract::The ATP-binding cassette (ABC) transporters constitute one of the largest families of proteins in evolution. The ATM1 gene of the yeast Saccharomyces cerevisiae encodes an ABC protein, which is localized to the mitochondrial inner membrane. A deletion of ATM1 results in the accumulation of up to a 30-fold excess of mi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.05.051
更新日期:2004-07-02 00:00:00
abstract::Biological activities of five synthetic lipid A analogues (D-glucosamine derivatives) were examined to elucidate the structure required for expression of the biological activities of endotoxin. Proclotting enzyme of horseshoe crab activation, interferon-inducing and tumor necrosis factor-inducing activities were signi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80131-3
更新日期:1984-02-27 00:00:00
abstract::Large conductance voltage-dependent and Ca(2+)-modulated K(+) channels play a crucial role in myometrium contractility. Western blots and immunocytochemistry of rat uterine sections or isolated cells show that MaxiK channel protein signals drastically decrease towards the end of pregnancy. Consistent with a transcript...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01394-0
更新日期:1999-11-05 00:00:00
abstract::Two fatty acid ethyl ester (FAEE) synthase isoenzymes purified from human myocardium were reported to be glutathione S-transferases (GST) [(1989) Proc. Natl. Acad. Sci. USA 86, 4470-4473; and (1989) J. Clin. Invest. 84, 1942-1946]. In the present study, the FAEE synthase activity of several purified and well character...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81438-t
更新日期:1990-11-26 00:00:00
abstract::We present evidence that the potent chloride channel blocker NPPB has protonophoric activity in the mitochondria and across the plasma membrane of phagocytic cells. The resting O2 consumption of murine peritoneal macrophages was stimulated up to 2.5-fold in the presence of NPPB, with a K0.5 of 15 microM. The stimulato...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80992-c
更新日期:1991-08-19 00:00:00
abstract::Apoferritin has been selectively labeled with a maleimide nitroxide derivative at Cys-126, located in the hydrophilic 3-fold channels. Titration of this derivative with Fe(II), which gives rise to the initial Fe(III)-apoferritin complex, produces, at low metal-to-protein ratios, a decrease of the intensity of the labe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80004-m
更新日期:1991-08-05 00:00:00
abstract::We have shown previously that the skeletal muscle ryanodine receptor mRNA of approximately 16,000 nucleotides codes 5,037 amino acid residues constituting the calcium release channel in skeletal muscle. In this study, RNA blot hybridization analysis shows that the brain contains an RNA species with an estimated size o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81547-d
更新日期:1993-05-10 00:00:00
abstract::Human aminopeptidase N carries an apical sorting signal on its ectodomain necessary for its correct transport to the apical membrane in Madin-Darby canine kidney cells. To determine whether the apical sorting signal is localized in the serine/threonine rich stalk or in the catalytic head group, anchor/stalk-minus amin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81039-o
更新日期:1992-08-10 00:00:00
abstract::Reversible phosphorylation of myosin subunits is observed in almost all eukaryotic cells. The data concerning sites and effects of phosphorylation on actin-activated ATPase activity of myosin and on its filament formation are described. These observations are discussed in terms of possible evolutionary trends and rule...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(86)80806-7
更新日期:1986-08-18 00:00:00
abstract::In Ruminococcus albus, 4-O-β-D-mannosyl-D-glucose phosphorylase (RaMP1) and β-(1,4)-mannooligosaccharide phosphorylase (RaMP2) belong to two subfamilies of glycoside hydrolase family 130. The two enzymes phosphorolyze β-mannosidic linkages at the nonreducing ends of their substrates, and have substantially diverse sub...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12105
更新日期:2016-03-01 00:00:00
abstract::The yeast PMR2/ENA1 gene encodes an ATPase involved in sodium extrusion and induced by NaCl. At low salt concentrations (0.3 M) induction is mediated by the HOG-MAP kinase pathway, a system activated by non-specific osmotic stress. At high salt concentrations (0.8 M) induction is mediated by the protein phosphatase ca...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00157-3
更新日期:1996-03-11 00:00:00
abstract::Phospholipase D (PLD) has been associated with necrosis. However, it is not clear whether PLD plays a causative role in this cellular process. We investigated the role of PLD in oxidative stress-induced necrosis of vascular smooth muscle cells (VSMCs). Pervanadate (hydrogen peroxide plus orthovanadate) but not hydroge...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03059-9
更新日期:2001-11-16 00:00:00
abstract::Recently the identity of the peptidyl-prolyl cis-trans isomerase (PPIase), which accelerates the cis/trans isomerization of prolyl peptide bonds and cyclophilin, the binding protein for the immunosuppressive drug Cyclosporin A (CsA), was discovered. The PPIase catalysis toward the substrate Suc-Ala-Phe-Pro-Phe-pNA has...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80766-v
更新日期:1991-06-17 00:00:00
abstract::The gap junctional intercellular communication mediated by Cx43 plays indispensable roles in both germ line development and postnatal folliculogenesis. In this study, we focused on the effect of follicle-stimulating hormone (FSH) on the Cx43 protein in rat primary granulosa cells and found that FSH stimulation elevate...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03441-5
更新日期:2002-11-06 00:00:00
abstract::Thioredoxin-interacting protein (Txnip) knockout (TKO) mice exhibit impaired response to fasting. Herein, we showed that activation of adenine monophosphate-activated protein kinase and cellular AMP levels were diminished in the heart and soleus muscle but not in gastrocnemius muscle of fasting TKO mice. Similarly, gl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.03.042
更新日期:2011-04-20 00:00:00