Abstract:
:We have used 19F NMR and CD spectra to study interactions of TFP with CaM under various conditions where no aggregation of TFP occurs. It was found that TFP is bound to CaM in the absence of Ca2+ with Ka greater than 4 X 10(4) M in terms of 19F NMR of TFP and that KCl markedly influences the interaction of TFP with CaM. It was also found that TFP causes quite strong induced CD bands corresponding with the absorption bands of the aromatic ring of TFP when TFP is bound to Ca2+-CaM. The induction of the CD bands was time-dependent and was composed of fast and slow phases.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Shimizu T,Hatano Mdoi
10.1016/0014-5793(83)80962-4subject
Has Abstractpub_date
1983-08-22 00:00:00pages
182-6issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(83)80962-4journal_volume
160pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Prolonged incubation of membrane fragments containing homogeneous (Na+ + K+)-ATPase with Mg2+, K+ and VO-3 at 4 degrees C resulted in formation of two-dimensional crystals of this enzyme with unit cell parameters: a = 66 A, b = 118 A, gamma = 108 degrees. The crystals correspond to the two-sided plane group p21. By co...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80430-0
更新日期:1985-10-07 00:00:00
abstract::Using top-of-the-line fold recognition methods, we assigned an oligonucleotide/oligosaccharide-binding (OB)-fold structure to a family of previously uncharacterized hypothetical proteins from several bacterial genomes. This novel family of bacterial OB-fold (BOF) proteins present in a number of pathogenic strains enco...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.04.086
更新日期:2004-06-04 00:00:00
abstract::The primary structure of a new sarafotoxin, SRTX-d, from the venom of Atractaspis engaddensis is described. SRTX-d differs from SRTX-b in two substitutions: Ile19 instead of Val and Thr2 instead of Ser. The toxicity of SRTX-d and its vasoconstriction potency are very low in comparison to SRTX-a and SRTX-b, whereas its...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81706-5
更新日期:1989-10-09 00:00:00
abstract::To detect and characterize ion channel activity in the nuclear envelope of a higher plant cell, we performed patch clamp experiments on nuclei isolated from coconut endosperm cells and on giant liposomes containing nuclear envelope fragments prepared from the same cells. An ion channel exhibiting a number of conductan...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80290-w
更新日期:1992-05-04 00:00:00
abstract::Apoptotic protease activating factor-1 (Apaf-1) is an adaptor molecule essential for caspase-9 activation. Subcellular analysis of Apaf-1 in NIH-3T3 fibroblasts and the immature murine B cell lymphoma WEHI-231 indicates that Apaf-1 is localized in the Golgi apparatus and cytoplasm. Bcl-2 overexpression in WEHI-231 cel...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02629-1
更新日期:2001-07-13 00:00:00
abstract::Alix and its homologs are involved in various phenomena such as endosomal protein-sorting and adaptation to stress conditions. In this study, we found that development of Dictyostelium discoideum Alix (DdAlix) deletion mutant (alx-) cells was impaired in alkaline pH environments. The fruiting body formation efficiency...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.02.036
更新日期:2005-03-14 00:00:00
abstract::Transactive response DNA-binding protein of 43 kDa (TDP-43) regulates RNA processing, including alternative splicing of tau exon 10. Pathological TDP-43 is hyperphosphorylated. However, how do the protein phosphatase(s) (PP) regulate TDP-43 phosphorylation is unclear. Here, we found that both PP1 and PP2A were coimmun...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12976
更新日期:2018-02-01 00:00:00
abstract::The nucleotide sequence of the dnaK operon cloned from Porphyromonas gingivalis revealed that the operon does not contain homologues of either dnaJ or grpE. However, there were two genes which encode small heat shock proteins immediately downstream from the dnaK and they were transcribed together with dnaK as one unit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00237-9
更新日期:1999-03-12 00:00:00
abstract::The protein RPE65 has an important role in retinoid processing and/or retinoid transport in the eye. Retinoids are involved in cell differentiation, embryogenesis and carcinogenesis. Since the kidney is known as an important site for retinoid metabolism, the expression of RPE65 in normal kidney and transformed kidney ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00606-7
更新日期:1999-06-11 00:00:00
abstract::Glycogen synthase from human and bovine polymorphonuclear leukocytes was purified to homogeneity. Rabbit antisera were raised against the two glycogen synthases and used for immunochemical analysis. Western blotting analysis showed that the subunit of glycogen synthase in crude homogenates of human and bovine leukocyt...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80653-7
更新日期:1988-03-28 00:00:00
abstract::The kinetic properties of dUTPase from equine infectious anemia virus (EIAV) were investigated. K(M) (1.1 +/- 0.1 microM) and k(cat) (25 s(-1)) were found to be independent of pH in the neutral pH range. Above pH 8.0, K(M) increases slightly. Below pH 6.0, the enzyme is rapidly deactivated. Detergent was found to enha...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00935-6
更新日期:1997-09-08 00:00:00
abstract::Glutathione peroxidase 3 (GPX3) is an important member of antioxidant enzymes for reducing reactive oxygen species and maintaining the oxygen balance. Gpx3 mRNA is strongly expressed in decidual cells from days 5 to 8 of pregnancy. After pregnant mice are treated with GPX inhibitor for 3 days, pregnancy rate is signif...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.02.043
更新日期:2014-05-02 00:00:00
abstract::Insecticidal proteins or delta-endotoxins of Bacillus thuringiensis are highly toxic to a wide range of agronomically important pests. The toxins are formed of three structural domains. The N-terminal domain is a bundle of eight alpha-helices and is implicated in pore formation in insect midgut epithelial membranes. A...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01157-6
更新日期:1999-09-17 00:00:00
abstract::A 3.3 kb cDNA encoding the complete amino acid sequence of a calcium/calmodulin regulated protein phosphatase has been isolated from a Drosophila eye disc cDNA library. The predicted protein of 560 amino acids (molecular mass 62 kDa) is 73-78% identical to human PP2B isoforms. The cDNA hybridised to the X-chromosome a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80398-6
更新日期:1994-02-14 00:00:00
abstract::Adiponectin and resistin, two recently identified adipocyte-specific secretory factors, are able to modulate insulin actions in target tissues. To investigate their expression and hormonal regulation in brown adipocytes, we used the brown adipocyte cell line T37i, which, beside uncoupling protein expression, secretes ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03697-9
更新日期:2002-12-18 00:00:00
abstract::The Escherichia coli K12 mutant gene, asnS40, coding for asparaginyl-tRNA synthetase (AsnRS) in the temperature-sensitive strain HO202, was isolated from genomic DNA using the Polymerase Chain Reaction. DNA sequencing revealed that the mutant enzyme differs from the wild-type AsnRS by two amino acids, but only the P23...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80106-q
更新日期:1992-03-24 00:00:00
abstract::The crystal structure of metagenome-derived LC9-RNase H1 was determined. The structure-based mutational analyses indicated that the active site motif of LC9-RNase H1 is altered from DEDD to DEDN. In this motif, the location of the second glutamate residue is moved from αA-helix to β1-strand immediately next to the fir...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.03.020
更新日期:2013-05-02 00:00:00
abstract::There is evidence that CCK-8 may interact with opioids and that both systems are probably implicated in pain modulation. In order to elucidate this relationship we sought to examine factors governing the movement of CCK-8 from the spinal cord into the extracellular space. We report that CCK-8 like immunoreactivity, as...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80723-9
更新日期:1989-07-03 00:00:00
abstract::Gangliosides and neutral glycolipids of muscles from normal and dystrophic chickens were studied. Total glycolipid content of the degenerating muscles was higher than the normal muscles. In addition, the myopathic muscles contained a ganglioside which was absent in the unaffected muscles from normal and dystrophic chi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(82)80768-0
更新日期:1982-12-27 00:00:00
abstract::The Methanocaldococcus jannaschii (formerly Methanococcus jannaschii) protein Mj0968 has been reported to represent a soluble P-type ATPase [Ogawa et al., FEBS Lett. 471 (2000) 99-102]. In this study, we report that the heterologously expressed Mj0968-His(10) protein exhibits high rates of phosphatase activity, wherea...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00372-7
更新日期:2003-05-22 00:00:00
abstract::Dun1p and Rad53p of the budding yeast Saccharomyces cerevisiae are members of a conserved family of cell cycle checkpoint protein kinases that contain forkhead-associated (FHA) domains. Here, we demonstrate that these FHA domains contain 130-140 residues, and are thus considerably larger than previously predicted by s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01392-2
更新日期:2000-04-14 00:00:00
abstract::A cell-free protein expression system was established that provides protein samples of adequate concentration and purity for direct NMR analysis. The Escherichia coli peptidyl-prolyl cis-trans isomerase PpiB was expressed in this system with dual amino acid-selective isotope labeling to identify the NMR signal from th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03048-x
更新日期:2002-07-31 00:00:00
abstract::Several peptidic and non-peptidic factors can modulate Na,K-ATPase activity, among them mainly inhibitors of this enzyme, ouabain being the most effective. In a very few cases only, activation of Na,K-ATPase by endogenous factors has been recorded. We have investigated the effect on Na,K-ATPase of a novel regulatory p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00407-2
更新日期:1994-05-23 00:00:00
abstract::The high affinity (low Km) cyclic GMP phosphodiesterase (PDE) is activated by GTP, while the cyclic AMP PDE is not. GTP and its hydrolysis-resistant analogue, guanylylimidodiphosphate (GppNHp), display a half-maximal stimulating effect at almost the same concentration (5 X 10(-6) M). The GTP stimulating effect is not ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80574-2
更新日期:1983-07-04 00:00:00
abstract::Mitogen-activated protein kinase (MAPK) cascades play an important role in mediating stress responses in plants. In Arabidopsis, 20 MAPKs have been identified and classified into four major groups (A-D). Little is known about the role of group C MAPKs. We have studied the activation of Arabidopsis subgroup C1 MAPKs (A...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.03.075
更新日期:2007-05-01 00:00:00
abstract::The effect of oligosaccharide carrying lipids on membrane fluidity has been investigated. Gangliosides GM1 and GQ1 were reconstituted into phosphatidylcholine bilayer membranes at low concentrations (less than 5 mol%). A strong fluidizing effect was observed leading to a suppression of the phase transition temperature...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80214-3
更新日期:1985-01-01 00:00:00
abstract::Pretreatment of tissues with potassium channel openers (KCO's) has been observed to be cytoprotective in a broad variety of insults. This phenomenon has been proposed to be intimately linked to activation of mitochondrial potassium channels which apparently modulate the mitochondrial production of reactive oxygen spec...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.01.013
更新日期:2010-05-17 00:00:00
abstract::The membrane-bound 3-chloro-4-hydroxyphenylacetate (Cl-OHPA) reductive dehalogenase from the chlorophenol-reducing anaerobe Desulfitobacterium hafniense was purified 11.3-fold to apparent homogeneity in the presence of the detergent CHAPS. The purified dehalogenase catalyzed the reductive dechlorination of Cl-OHPA to ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01114-4
更新日期:1998-10-02 00:00:00
abstract::Phospholipase D was first described in plant tissue but has recently been shown to occur in mammalian cells where it is activated by cell surface receptors. Its mode of activation by receptors in unclear. Biochemical studies suggest that it may occur downstream of other effector proteins and that small GTP-dependent r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00366-x
更新日期:1999-04-09 00:00:00
abstract::To determine the amino acid residues required for the signal-transducing activity of the human c-Ha-Ras protein, we introduced point mutations at residues 45-54 near the 'effector region' (residues 32-40). We transfected PC12 cells with these mutant genes and also micro-injected the mutant proteins, bound with an unhy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80665-p
更新日期:1991-12-09 00:00:00