Abstract:
:A 3.3 kb cDNA encoding the complete amino acid sequence of a calcium/calmodulin regulated protein phosphatase has been isolated from a Drosophila eye disc cDNA library. The predicted protein of 560 amino acids (molecular mass 62 kDa) is 73-78% identical to human PP2B isoforms. The cDNA hybridised to the X-chromosome at cytological position 14D1-4. Two transcripts of 3.5 kb and 3.0 kb were expressed during embryonic development, their levels being highest in the early embryo. The larger transcript was also clearly present in adult females. This pattern of expression indicates a role for calcium/calmodulin regulated protein phosphatase in embryonic development.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Brown L,Chen MX,Cohen PTdoi
10.1016/0014-5793(94)80398-6subject
Has Abstractpub_date
1994-02-14 00:00:00pages
124-8issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(94)80398-6journal_volume
339pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The fluorescence dye 1-anilinonaphthalene-8-sulfonate (ANS) was used as a probe of non-polar binding sites in 4-aminobutyrate aminotransferase. ANS binds to a single binding site of the dimeric protein with a Kd of 6 microM. Nanosecond emission anisotropy measurements were performed on the ANS-enzyme in an effort to d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80971-5
更新日期:1983-08-22 00:00:00
abstract::We have used HPLC techniques to investigate the effects of gp120 upon inositol phosphate turnover in Jurkat E6-1 CD4+ T-cells, to pursue previous reports that this viral coat protein: (a) inhibits receptor-activated inositol phosphate release; (b) stimulates basal inositol phosphate release; (c) inhibits inositol poly...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00880-6
更新日期:1997-08-11 00:00:00
abstract::The rapid conversion of the carotenoid violaxanthin to zeaxanthin via antheraxanthin (xanthophyll cycle) in potato leaves exposed at 23 degrees C to a strong white light of 2000 microE.m-2.s-1 was associated with a slight inhibition of photosynthetic electron transport (as estimated from chlorophyll fluorescence measu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01028-5
更新日期:1994-10-17 00:00:00
abstract::Polymyxin B (PXB), a cyclic peptide antibiotic, in concentrations 0.1-3.0 mg/ml (0.08-4.0 mmol/l), inhibited the K+ efflux induced by opening of the Ca2+-activated K+ channel (the Gárdos effect) in intact human red blood cells. The inhibition was observed when the Gárdos effect was elicited by Ca2+ in the presence of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81152-3
更新日期:1987-12-10 00:00:00
abstract::Metabolic control theory is extended to include channelled metabolism in general. A simple relationship between the flux control by the enzymes and the degree of metabolite channelling is derived. This relationship suggests experiments in which modulation of gene expression allows one to quantify channelling. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81660-r
更新日期:1993-03-29 00:00:00
abstract::Cold-stable and cold-labile microtubules were prepared by two cycles of assembly and disassembly and two periods of exposure to cold. The cold-labile preparations were shown to contain a higher proportion of a high molecular mass microtubule-associated protein (MAP 2) than cold-stable preparations. The cold-stable pre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80341-5
更新日期:1984-04-24 00:00:00
abstract::Mitochondrial fatty acid synthesis is catalyzed by a dissociated fatty acid synthase similar to those of plant plastids and bacteria. The crystal structure of a mitochondrial beta-ketoacyl-[acyl carrier protein] synthase (mtKAS), namely that from Arabidopsis thaliana, has been determined for the first time. This enzym...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.10.007
更新日期:2004-11-05 00:00:00
abstract::Adaptation to hypoxia is essential for tumor progression. Transcriptional activation of hypoxia-regulated genes is mediated by hypoxia-inducible factor 1 (HIF-1), a heterodimer of HIF-1alpha and ARNT (Ah receptor nuclear translocator; HIF-1beta). Using representational difference analysis, we identified three novel hy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03475-0
更新日期:2002-10-23 00:00:00
abstract::Alix and its homologs are involved in various phenomena such as endosomal protein-sorting and adaptation to stress conditions. In this study, we found that development of Dictyostelium discoideum Alix (DdAlix) deletion mutant (alx-) cells was impaired in alkaline pH environments. The fruiting body formation efficiency...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.02.036
更新日期:2005-03-14 00:00:00
abstract::A cDNA of a novel form of type V adenylyl cyclase has been cloned from rabbit myocardium using oligonucleotide probes derived from peptides that were produced by enzymatic cleavage of purified heart cyclase. A corresponding mRNA (6 kb) has been detected in rabbit myocardial tissue by Northern blot analysis. The cDNA e...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80279-3
更新日期:1994-02-07 00:00:00
abstract::The in vivo toxicity of vanadium(V) has been found to correlate with the depletion of cellular glutathione and related non-protein thiols. With a view to understanding the mechanism for this observation, we have investigated the oxidation of glutathione, cysteine N-acetylcysteine and penicillamine by vanadium(V), usin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80402-5
更新日期:1990-10-01 00:00:00
abstract::Upon in vitro translation of mRNAs from slow (soleus) muscles of the mouse a hitherto undescribed translation product has been detected that was absent from fast skeletal muscles and was termed p19/6.8 according to its position in 2-dimensional gels. mRNA for p19/6.8 was also found in the ventricle of the heart. p19/6...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80669-0
更新日期:1988-03-28 00:00:00
abstract::Regulation of Na(+)-dependent glutamate transport was studied in isolated luminal and abluminal plasma membranes derived from the bovine blood-brain barrier. Abluminal membranes have Na(+)-dependent glutamate transporters while luminal membranes have facilitative transporters. This organization allows glutamate to be ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.06.097
更新日期:2006-08-07 00:00:00
abstract::The main role of voltage-gated proton channels (Hv1) is to extrude protons from the intracellular milieu when, mediated by different cellular processes, the H(+) concentration increases. Hv1 are exquisitely selective for protons and their structure is homologous to the voltage sensing domain (VSD) of other voltage-gat...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2015.08.003
更新日期:2015-11-14 00:00:00
abstract::This paper reviews our present knowledge of sphingomyelinases as enzymes, and as enzymes acting on a membrane constituent lipid, sphingomyelin. Six types of sphingomyelinases are considered, namely acidic, secretory, Mg(2+)-dependent neutral, Mg(2+)-independent neutral, alkaline, and bacterial enzymes with both phosph...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(02)03482-8
更新日期:2002-10-30 00:00:00
abstract::Ascorbic acid uptake was investigated in isolated, plated human neutrophils using high-performance liquid chromatography with coulometric electrochemical detection. Freshly isolated neutrophils contained 1.3 mM ascorbic acid and accumulated significantly greater amounts when physiologic concentrations of the vitamin w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80077-v
更新日期:1990-01-15 00:00:00
abstract::Zona pellucida (ZP) binding proteins from boar spermatozoa were compared with antigens recognized by ACR.2 and ACR.3 monoclonal antibodies. The ZP binding proteins of 55, 53, 45 and 38 kDa are identical with various forms of boar acrosin immunologically detected by ACR.2 antibody. The ZP binding proteins of 17 and 18 ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80258-k
更新日期:1990-05-21 00:00:00
abstract::MicroRNAs (miRNA) play an important role in tumorigenesis, proliferation, and differentiation. Altered miRNA expression in cancer indicates that miRNAs can function as tumor suppressors or oncogenes. MiR-449c downregulation in non-small cell lung cancer (NSCLC) compared with normal lung tissues was investigated in thi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.03.006
更新日期:2013-05-02 00:00:00
abstract::A chloroperoxidase gene from Pseudomonas pyrrocinia was cloned into Escherichia coli using the cosmid vector pJB8. The gene coding for the chloroperoxidase could be localized to a 1.5 kb fragment of DNA which was subcloned into the high-copy-number plasmid pUC18. In one subclone increased halogenating activity could b...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80505-2
更新日期:1988-10-10 00:00:00
abstract::E. coli trigger factor is an abundant cytosolic protein originally identified by its ability to maintain the precursor of a secretory protein in a translocation competent form. Recent studies shed new light on the function of this protein. Trigger factor was found to be a peptidyl-prolyl-cisltrans-isomerase capable of...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(96)00582-0
更新日期:1996-06-24 00:00:00
abstract::In the present study, we have explored the impact of deleting a single allele of PDK1 in T cell progenitors on alpha/beta and gamma/delta T cell development. The data show that deleting a single allele of PDK1 allows differentiation of alpha/beta T cells but prevents their proliferative expansion in the thymus. Accord...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.03.022
更新日期:2006-04-03 00:00:00
abstract::The protein kinase C activator, phorbol-12-myristate-13-acetate (PMA), augments the cyclic AMP accumulation induced by forskolin in pheochromocytoma (PC 12) cells with an EC50 value of 14 nM, while having no effect on basal values. At a concentration of 100 nM PMA markedly augmented the magnitude of the forskolin resp...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80227-7
更新日期:1986-02-03 00:00:00
abstract::The p53 tumor suppressor coordinates a multitude of cellular and organismal processes and exerts its activities mainly by activation of gene transcription. Here we describe the transcriptional activation of ectodysplasin A2 receptor (EDA2R) by p53 in a variety of cell types and tissues. We demonstrate that treatment o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.04.058
更新日期:2010-06-03 00:00:00
abstract::Rat liver fructose 1,6-bisphosphatase can be protected against partial inactivation by N-ethylmaleimide by low concentrations of fructose 2,6-bisphosphate or high concentrations of fructose 1,6-bisphosphate. The partially inactivated enzyme has a much reduced sensitivity to high substrate inhibition and has lost the s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80946-6
更新日期:1983-08-22 00:00:00
abstract::Macrophages contain arginase and an inducible NO synthase, demonstrated by using L-arginine, the common substrate, for production of both nitric oxide and urea. Arginase was inhibited by nitrite, the stable end product of NO. This inhibition was non-competitive, and could not be explained by the reaction of nitrite wi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00659-x
更新日期:1996-07-22 00:00:00
abstract::We introduced rat ornithine transcarbamylase (OTC) gene into OTC-deficient spf-ash mice by mating spf-ash heterozygotes with transgenic mice which carried recombinant DNA composed of 1.3 kb of the 5' flanking region of the gene fused onto rat OTC cDNA. The liver OTC activity of hemizygous spf-ash mice which carried th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80148-v
更新日期:1991-02-25 00:00:00
abstract::Lysophosphatidic acid (LPA) exerts multiple biological functions through G protein-coupled receptors (EDG2/LPA(1), EDG4/LPA(2), and EDG7/LPA(3)) and is present in serum where it is associated with albumin. In this study we examined LPA activity in various biological fluids by measuring the LPA-induced increase in the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02976-9
更新日期:2002-07-17 00:00:00
abstract::miR398 links expression of the three major chloroplast copper proteins, plastocyanin, CCS1 and Csd2, to copper availability. miR398 abundance was stronger plastocyanin-controlled in accessions from cold and continental habitats (Kas-1, Ms-0, WS) than in Cvi-0 and Col-0. Target gene regulation was broken for Csd2 in Cv...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.07.049
更新日期:2012-09-21 00:00:00
abstract::The photosynthetic reaction center is one of the most complicated molecular complexes. Transducing photon energy to a transmembrane electrochemical potential difference for protons, it is the direct or indirect energy source for virtually all life. We show here that it operates in a simple, battery-like manner, with a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03210-0
更新日期:2002-01-02 00:00:00
abstract::Spermiogenesis is a dynamic process leading to alterations in cell morphology. In spermiogenesis, the roles of the histone chaperones are largely unknown. Here, I report the unexpected roles of two Nap family proteins, CG5017/Hanabi and nucleosome assembly protein 1 (Nap1) in Drosophila. Hanabi is mainly localized in ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.02.019
更新日期:2013-04-02 00:00:00