Sphingomyelinases: enzymology and membrane activity.

abstract：:We have generated a mouse model for hepatic ischemia in which surgical subcutaneous transposition of the spleen allows hepatic ischemia to be applied without affecting other tissues. Using this mouse model we investigated the relationship between the length of ischemic periods in the liver and subsequent liver functio...

journal_title：FEBS letters

authors： Onishi I,Tani T,Hashimoto T,Shimizu K,Yagi M,Yamamoto K,Yoshioka K

更新日期：1997-12-29 00:00:00

abstract：:Stimulation of the bicarbonate dehydration reaction in thylakoid suspension under conditions of saturating light at pH 7.6-8.0 was discovered. This effect was inhibited by nigericin or the lipophilic carbonic anhydrase (CA) inhibitor ethoxyzolamide (EZ), but not by the hydrophilic CA inhibitor, acetazolamide. It was s...

journal_title：FEBS letters

authors： Moskvin OV,Ivanov BN,Ignatova LK,Kollmeier MA

更新日期：2000-03-31 00:00:00

abstract：:Human SRC encodes the non-receptor tyrosine kinase pp60(c-Src), which is activated in many human colon cancer cell lines (HCCLs) and tumors. We found that both c-Src protein and mRNA levels were elevated in a subset of HCCLs. Increased c-Src mRNA and protein levels correlated strongly with increased c-Src kinase activ...

journal_title：FEBS letters

authors： Dehm S,Senger MA,Bonham K

更新日期：2001-01-05 00:00:00

abstract：:Monoclonal antibodies (mAbs) against cell surface antigens and receptors are instrumental in defining specific membrane markers. mAbs GF 26.7.3 and MF 25.1 against human neutrophils modulated the activation mechanism of superoxide anion production induced by formyl-peptide and PMA in all subject. However, treatment wi...

journal_title：FEBS letters

authors： Traniello S,Spisani S,Gavioli R,Dovigo L,Baricordi RO,Sensi A,Damiani G

更新日期：1986-08-11 00:00:00

abstract：:Dipeptidylaminopeptidase IV, a plasma membrane-bound glycoprotein, is characterized by an intramolecular heterogeneous turnover of the protein backbone and carbohydrate chain. The faster turnover of the latter is restricted only to the outer sugars. The inner core sugars D-mannose and N-acetyl-D-glucosamine turn over ...

journal_title：FEBS letters

authors： Volk BA,Kreisel W,Köttgen E,Gerok W,Reutter W

更新日期：1983-10-31 00:00:00

abstract：:There is considerable epidemiological evidence that tea drinking lowers the risk of heart disease. However, the mechanism by which tea can be protective is unknown. Hamsters were fed a normal or high cholesterol diet for 2 weeks and drank green or black tea ad libitum. The plasma lipid profile was significantly improv...

journal_title：FEBS letters

authors： Vinson JA,Dabbagh YA

更新日期：1998-08-14 00:00:00

abstract：:The two subunits of gamma-glutamyl transpeptidase (EC 2.3.2.2) are derived from a single-chain glycosylated precursor. A small fraction of the propeptide survives proteolytic processing in the rat kidney and has been purified by an immunoaffinity technique. The propeptide contains determinants for both the subunits an...

journal_title：FEBS letters

authors： Tate SS

更新日期：1986-01-01 00:00:00

abstract：:Accumulation of hyperphosphorylated Tau is associated with a number of neurodegenerative diseases collectively known as tauopathies. Differences in clinical and cognitive profiles among them suggest differential sensitivity of neuronal populations to Tau levels, phosphorylation and mutations. We used tissue specific e...

journal_title：FEBS letters

authors： Grammenoudi S,Kosmidis S,Skoulakis EM

更新日期：2006-08-21 00:00:00

abstract：:The galactose transport system of Saccharomyces cerevisiae consists of one component which shows a Km value of approx. 4mM in growing cells. A rapid and irreversible inactivation of this transport is detected on impairment of protein synthesis. This inactivation shows the following characteristics: (i) it is due to ch...

journal_title：FEBS letters

authors： DeJuan C,Lagunas R

更新日期：1986-10-27 00:00:00

abstract：:Binding of the thiazolyl peptide antibiotic thiostrepton to the GTPase domain of 23S rRNA involves a few crucial nucleotides, notably A1067 (E. coli). Small RNA transcripts were prepared corresponding to the GTPase domain of the plastid 23S rRNA and the two forms of cytosolic 28S rRNAs found in the human malaria paras...

journal_title：FEBS letters

authors： Clough B,Strath M,Preiser P,Denny P,Wilson IR

更新日期：1997-04-07 00:00:00

abstract：:In human pancreatic islets an increase in the glucose concentration from 3 to 20 mM raised the free cytoplasmic Ca2+ concentration [( Ca2+]i), an effect being reversible upon withdrawal of the sugar. Depolarization with a high concentration of K+ or the sulphonylurea tolbutamide also raised [Ca2+]i. Addition of extrac...

journal_title：FEBS letters

authors： Kindmark H,Köhler M,Nilsson T,Arkhammar P,Wiechel KL,Rorsman P,Efendić S,Berggren PO

更新日期：1991-10-21 00:00:00

abstract：:The interactions between the polyanionic ligands phosphate and sulphate and the type II dehydroquinases from Streptomyces coelicolor and Mycobacterium tuberculosis have been characterised using a combination of structural and kinetic methods. From both approaches, it is clear that interactions are more complex in the ...

journal_title：FEBS letters

authors： Evans LD,Roszak AW,Noble LJ,Robinson DA,Chalk PA,Matthews JL,Coggins JR,Price NC,Lapthorn AJ

更新日期：2002-10-23 00:00:00

abstract：:Turnip yellow mosaic virus (TYMV) is a positive-strand RNA virus able to infect Arabidopsis thaliana. To establish a TYMV infection system in Arabidopsis cell culture, TYMV replicons with the capsid protein gene replaced by a reporter gene expressing the Sh ble protein conferring zeocin resistance were used to transfe...

journal_title：FEBS letters

authors： Camborde L,Tournier V,Noizet M,Jupin I

更新日期：2007-01-23 00:00:00

abstract：:Several lines of evidence led to the conclusion that mammalian ribosomal protein synthesis is a highly organized biological process in vivo. A wealth of data support the concept according to which tRNA aminoacylation, formation of the ternary complex on EF1A and delivery of aminoacyl-tRNA to the ribosome is a processi...

journal_title：FEBS letters

authors： Mirande M

更新日期：2010-01-21 00:00:00

abstract：:Nitric oxide (.NO) synthase (NOS) activity in subcellular fractions from cultured endothelial cells (EC) and lipopolysaccharide-activated J774.2 monocyte/macrophages was investigated by monitoring the .NO-mediated increase in intracellular cyclic GMP in LLC-PK1 pig kidney epithelial cells. The constitutive NOS in EC (...

journal_title：FEBS letters

authors： Hecker M,Walsh DT,Vane JR

更新日期：1991-12-09 00:00:00

abstract：:The contents of sterol derivatives (sterols + esters, glycosides and acylated glycosides) have been determined using thin layer chromatography in leaves and bulky and organs from carrots (Daucus carota L.) potato (Solanum tuberosum L.), onion (Allium cepa L.) two varieties of radish (Raphanus sativus L.) and Gentiana ...

journal_title：FEBS letters

authors： Eichenberger W,Grob EC

更新日期：1970-12-01 00:00:00

abstract：:The two activators of protein kinase C, oleoylacetylglycerol (OAG) and dioctanoylglycerol (DOG), are able to induce a concentration-dependent rise in cytoplasmic free Ca2+ concentration in gel-filtered human platelets, detected as an increase in quin2 fluorescence. The phorbol ester phorbol-12-myristate-13-acetate (PM...

journal_title：FEBS letters

authors： Bruns C,Schächtele C,Marmé D

更新日期：1987-08-31 00:00:00

abstract：:Nine analogs of fusion peptide of influenza virus hemagglutinin whose membrane perturbation activity has been thoroughly tested [Murata et al. (1992) Biochemistry 31, 1986-1992; Murata et al. (1993) Biophys. J. 64, 724-734] were characterized by molecular modeling techniques with the aim of delineating any specific st...

journal_title：FEBS letters

authors： Efremov RG,Nolde DE,Volynsky PE,Chernyavsky AA,Dubovskii PV,Arseniev AS

更新日期：1999-11-26 00:00:00

abstract：:An experimental procedure is described for the reproducible production of hybridoma cells using the electrofusion technique. High yields can be obtained when fusion is performed in isotonic inositol solutions containing Ca2+ and Mg2+ in a ratio of 1:5 in the millimolar range. The hybridoma cells are transferred 10 min...

journal_title：FEBS letters

authors： Vienken J,Zimmermann U

更新日期：1985-03-25 00:00:00

abstract：:Glucosamine-6-phosphate (GlcN6P) N-acetyltransferase 1 (GNA1) is a key enzyme in the pathway toward biosynthesis of UDP-N-acetylglucosamine, an important donor substrate for N-linked glycosylation. GNA1 catalyzes the formation of N-acetylglucosamine-6-phosphate (GlcNAc6P) from acetyl-CoA (AcCoA) and the acceptor subst...

journal_title：FEBS letters

authors： Wang J,Liu X,Liang YH,Li LF,Su XD

更新日期：2008-09-03 00:00:00

abstract：:Different bioinformatics methods illuminate different aspects of protein function, from specific catalytic activities to broad functional categories. Here, a triple-pronged approach to predict function for a domain of unknown function, DUF2086, is applied. Distant homology to characterised enzymes and conservation of ...

journal_title：FEBS letters

authors： Mello LV,Rigden DJ

更新日期：2012-11-02 00:00:00

abstract：:As an attempt to investigate the dynamic interactions between plasma serine protease, coagulation factor VIIa (VIIa) and its cofactor, tissue factor (TF), we performed normal mode analysis (NMA) of the complex of VIIa with soluble TF (the extracellular part of TF; sTF). We compared fluctuations of Calpha atoms of VIIa...

journal_title：FEBS letters

authors： Soejima K,Kurihara Y,Kamiya K,Umeyama H

更新日期：1999-12-10 00:00:00

abstract：:The importance of HNF1 binding proteins for intestinal aminopeptidase N expression was investigated using the Caco-2 cell-line. Aminopeptidase N promoter activity in Caco-2 cells depends on the HNF1 element (positions -85 to -58) and co-transfection with an HNF1 alpha expression vector demonstrates a direct activation...

journal_title：FEBS letters

authors： Olsen J,Laustsen L,Troelsen J

更新日期：1994-04-11 00:00:00

abstract：:Histone H1(0) is found in tissues with little or no cellular proliferation and has been shown to accumulate during cellular terminal differentiation. Two subtypes of H1(0), H1 a and H1 b, are present in any tissue where the protein has been detected. We report here the first evidence of an age-dependent change in the ...

journal_title：FEBS letters

authors： Piña B,Suau P

更新日期：1987-01-05 00:00:00

abstract：:Single molecule localization microscopy (SMLM), which can provide up to an order of magnitude improvement in spatial resolution over conventional fluorescence microscopy, has the potential to be a highly useful tool for quantitative biological experiments. It has already been used for this purpose in varied fields in ...

journal_title：FEBS letters

authors： Shivanandan A,Deschout H,Scarselli M,Radenovic A

更新日期：2014-10-01 00:00:00

abstract：:Prepro-vasoactive intestinal peptide (prepro VIP) was expressed in NIH 3T3 cells, and the prepro VIP-derived peptides produced by the cells were analyzed by chromatography combined with sequence-specific radio-immunoanalysis. In accordance with what has previously been reported on processing in non-endocrine cell line...

journal_title：FEBS letters

authors： Wulff BS,Georg B,Fahrenkrug J

更新日期：1994-03-14 00:00:00

abstract：:The antibacterial activity of many small, positively charged peptides and proteins is based on pore formation in lipid bilayers. It is here proposed to arise from an electroporation effect. This hypothesis is supported by calculations of the electrostatic potential of NK-lysin associated to a membrane. For a significa...

journal_title：FEBS letters

authors： Miteva M,Andersson M,Karshikoff A,Otting G

更新日期：1999-11-26 00:00:00

abstract：:The yeast integral plasma membrane protein Ist2 belongs to a group of membrane proteins which are synthesized from localized mRNAs. The protein reaches the plasma membrane via the ER on a route operating independently of the classical secretory pathway. We have identified a complex peptide-sorting signal located at th...

journal_title：FEBS letters

authors： Franz A,Maass K,Seedorf M

更新日期：2007-02-06 00:00:00

abstract：:Transgenically introduced laminin (LN) alpha1 chain prevents muscular dystrophy in LNalpha2 chain deficient mice. We now report increased integrin alpha7Bbeta1D synthesis in dystrophic LNalpha2 chain deficient muscle. Yet, immunofluorescence demonstrated a reduced expression of integrin alpha7B subunit at the sarcolem...

journal_title：FEBS letters

authors： Gawlik KI,Mayer U,Blomberg K,Sonnenberg A,Ekblom P,Durbeej M

更新日期：2006-03-20 00:00:00

abstract：:The remarkable stability of c-myc during oogenesis contrasts with its degradation during the early developmental period in Xenopus laevis. Three evolutionary conserved motifs found in the 3'-untranslated region of Xenopus c-myc RNAs have been analyzed for a possible role in c-myc RNA degradation. No specific degradati...

journal_title：FEBS letters

authors： Tchang F,Vriz S,Méchali M

更新日期：1991-10-21 00:00:00