Abstract:
:Stimulation of the bicarbonate dehydration reaction in thylakoid suspension under conditions of saturating light at pH 7.6-8.0 was discovered. This effect was inhibited by nigericin or the lipophilic carbonic anhydrase (CA) inhibitor ethoxyzolamide (EZ), but not by the hydrophilic CA inhibitor, acetazolamide. It was shown that the action of EZ is not caused by an uncoupling effect. It was concluded that thylakoid CA is the enzyme utilizing the light-generated proton gradient across the thylakoid membrane thus facilitating the production of CO(2) from HCO(3)(-) and that this enzyme is covered from the stroma side of thylakoids by a lipid barrier.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Moskvin OV,Ivanov BN,Ignatova LK,Kollmeier MAdoi
10.1016/s0014-5793(00)01328-4keywords:
subject
Has Abstractpub_date
2000-03-31 00:00:00pages
375-7issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(00)01328-4journal_volume
470pub_type
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