Promoted evolution of a shortened variant of heme A synthase in the membrane of Bacillus subtilis.

abstract：:Here we show that increased amount of secondary structure is acquired in the folded states of two structurally-different proteins (alpha-helical VlsE and alpha/beta flavodoxin) in the presence of macromolecular crowding agents. The structural content of flavodoxin and VlsE is enhanced by 33% and 70%, respectively, in ...

journal_title：FEBS letters

authors： Perham M,Stagg L,Wittung-Stafshede P

更新日期：2007-10-30 00:00:00

abstract：:We have recently demonstrated that myocardial adaptation to ischemia triggers a tyrosine kinase regulated signaling pathway leading to the translocation and activation of p38 MAP kinase and MAPKAP kinase 2. Since oxidative stress is developed during ischemic adaptation and since free radicals have recently been shown ...

journal_title：FEBS letters

authors： Maulik N,Sato M,Price BD,Das DK

更新日期：1998-06-16 00:00:00

abstract：:Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system characterized by widespread inflammation, focal demyelination and a variable degree of axonal and neuronal loss. Ionic conductances regulate T cell activation as well as neuronal function and thus have been found to play a crucial ...

journal_title：FEBS letters

authors： Ehling P,Bittner S,Budde T,Wiendl H,Meuth SG

更新日期：2011-12-01 00:00:00

abstract：:Bacterial DNA supercoiling is controlled by balancing the supercoiling activity of DNA gyrase and the relaxing activity of DNA topoisomerase I. We have characterized the gyrB gene from a top A deletion mutant of Escherichia coli (DM800) that has a compensatory mutation in gyrB, lowering the activity of gyrase 10-fold,...

journal_title：FEBS letters

authors： McEachern F,Fisher LM

更新日期：1989-08-14 00:00:00

abstract：:Streptomyces lividans 1326-9, a bldA+ strain, and its bldA39 mutant derivative J1725 were transformed with a cosmid containing the pur cluster, which determines the puromycin biosynthetic pathway from Streptomyces alboniger. bldA+ transformants produced puromycin in typical amounts, whereas bldA39 transformants did so...

journal_title：FEBS letters

authors： Tercero JA,Espinosa JC,Jiménez A

更新日期：1998-01-16 00:00:00

abstract：:The amino acid sequence and part of the DNA sequence of a glutamic acid-specific serine protease from Streptomyces griseus is reported. This protease is shown to be homologous with other serine proteases. An improved purification protocol for this enzyme is described. ...

journal_title：FEBS letters

authors： Svendsen I,Jensen MR,Breddam K

更新日期：1991-11-04 00:00:00

abstract：:The phosphopeptide Ser (P)-Ser(P)-Ser-(P)-Glu-Glu-Ser22-Ile-Thr, reproducing the 17-24 segment of beta-casein A2 including the seryl residue (Ser-22) which is targeted by casein kinase-1 was synthesized and used as model substrate for this enzyme. Its phosphorylation efficiency is actually higher than that of intact b...

journal_title：FEBS letters

authors： Meggio F,Perich JW,Reynolds EC,Pinna LA

更新日期：1991-06-03 00:00:00

abstract：:The orientation of the transition dipole moments in the ground state and the M-intermediate of bacteriorhodopsin were determined by time-resolved and steady-state polarized absorption spectroscopy on samples of oriented immobilized purple membranes. The angle between the transition dipole moment and the membrane norma...

journal_title：FEBS letters

authors： Otto H,Heyn MP

更新日期：1991-11-18 00:00:00

abstract：:Dicarbonyl/L-xylulose reductase (DCXR) converts l-xylulose into xylitol, and reduces various α-dicarbonyl compounds, thus performing a dual role in carbohydrate metabolism and detoxification. In this study, we identified DHS-21 as the only DCXR ortholog in Caenorhabditis elegans. The dhs-21 gene is expressed in variou...

journal_title：FEBS letters

authors： Son le T,Ko KM,Cho JH,Singaravelu G,Chatterjee I,Choi TW,Song HO,Yu JR,Park BJ,Lee SK,Ahnn J

更新日期：2011-05-06 00:00:00

abstract：:Chronic inflammation associated with tumor necrosis factor (TNF)-alpha and reactive oxygen species (ROS) is the hallmark of tuberculosis. Mycobacterium tuberculosis (MTB) directly stimulates human monocytes to secrete TNF-alpha. We show the augmented expression of TNF-alpha mRNA in MTB-infected monocytes by cellular a...

journal_title：FEBS letters

authors： Hasan N,Yusuf N,Toossi Z,Islam N

更新日期：2006-05-01 00:00:00

abstract：:Activity of tonoplast slow vacuolar (SV, or TPC1) channels has to be under a tight control, to avoid undesirable leak of cations stored in the vacuole. This is particularly important for salt-grown plants, to ensure efficient vacuolar Na(+) sequestration. In this study we show that choline, a cationic precursor of gly...

journal_title：FEBS letters

authors： Pottosin I,Bonales-Alatorre E,Shabala S

更新日期：2014-11-03 00:00:00

abstract：:The possible participation of calmodulin in the activation of store-operated Ca2+ entry (SOC) in single rat hepatocytes was investigated microspectrofluorimetrically. SOC was triggered after discharging intracellular Ca2+ stores using the endoplasmic reticulum Ca2+-ATPase inhibitor thapsigargin in the absence of exter...

journal_title：FEBS letters

authors： Cao Y,Chatton JY

更新日期：1998-03-06 00:00:00

abstract：:Glycogen phosphorylase b at concentrations close to those found in skeletal muscle interacts with sarcoplasmic reticulum membranes, but not with liposomes made of lipids extracted from these membranes, and is inhibited upon binding to the membrane. The interaction of glycogen phosphorylase with the sarcoplasmic reticu...

journal_title：FEBS letters

authors： Cuenda A,Centeno F,Gutierrez-Merino C

更新日期：1991-06-03 00:00:00

abstract：:Escherichia coli phosphofructokinase-2 (Pfk-2) is a homodimer whose subunits consist of a large domain and an additional beta-sheet that provides the interfacial contacts between the subunits, creating a beta-barrel flattened-like structure with the adjacent subunit's beta-sheet. To determine how the structural organi...

journal_title：FEBS letters

authors： Baez M,Babul J

更新日期：2009-06-18 00:00:00

abstract：:Parathyroid hormone (PTH) binds to its receptor (PTH 1 receptor, PTH1R) and activates multiple pathways. The PTH1R, a class b GPCR, contains consensus calmodulin-binding motifs. The PTH1R cytoplasmic tail interacts with calmodulin in a calcium-dependent manner via the basic 1-5-8-14 motif. Calcium-dependent calmodulin...

journal_title：FEBS letters

authors： Mahon MJ,Shimada M

更新日期：2005-01-31 00:00:00

abstract：:10Sa RNA is a bacterial small stable RNA, in which the 5'- and 3'-end sequences are folded into a tRNA-like structure. The RNA accepts alanine in vitro, and interacts with 70S ribosomes in the cells. In this study, we examined the ribosome-binding properties of Escherichia coli 10Sa RNA in vivo, and found that the ami...

journal_title：FEBS letters

authors： Tadaki T,Fukushima M,Ushida C,Himeno H,Muto A

更新日期：1996-12-16 00:00:00

abstract：:Immunoprecipitated p100Gag-Mil protein kinase from MH2-transformed quail embryo fibroblasts is associated with an 89 kDa protein. The molar ratio between p89 and Gag-Mil in the immunocomplex is 0.72, indicating that the majority of Gag-Mil is complexed with p89. During mitosis part of Gag-Mil is shifted to a form with...

journal_title：FEBS letters

authors： Lovrić J,Bischof O,Moelling K

更新日期：1994-04-18 00:00:00

abstract：:LUSH is a soluble odorant-binding protein of the fruit fly Drosophila melanogaster. Mutants not expressing this protein have been reported to lack the avoidance behaviour, exhibited by wild type flies, to high concentrations of ethanol. Very recently, the three-dimensional structure of LUSH complexed with short-chain ...

journal_title：FEBS letters

authors： Zhou JJ,Zhang GA,Huang W,Birkett MA,Field LM,Pickett JA,Pelosi P

更新日期：2004-01-30 00:00:00

abstract：:Moloney murine leukemia virus reverse transcriptase (RT) selectively uses deoxyribonucleotides over ribonucleotides (rNTPs) as substrates. Substitution of F155 with valine (F155V) was previously found to increase the enzyme's affinity for rNTPs, though without affecting the V(max) for catalysis, and thereby conferred ...

journal_title：FEBS letters

authors： Liu S,Goff SP,Gao G

更新日期：2006-02-20 00:00:00

abstract：:Decades of neuroscience research have shed light on the hippocampus as a key structure for the formation of episodic memory. The hippocampus is divided into distinct subfields - CA1, CA2 and CA3. While accumulating evidence points to cellular and synaptic heterogeneity within each subfield, this heterogeneity has not ...

journal_title：FEBS letters

authors： Igarashi KM,Ito HT,Moser EI,Moser MB

更新日期：2014-08-01 00:00:00

abstract：:The effects of temperature on the gamma-aminobutyric acid (GABA) uptake and on the presteady-state and transport-associated currents of the GABA cotransporter, rat gamma-aminobutyric acid transporter 1 (rGAT1), have been studied using heterologous oocyte expression and voltage-clamp. Increasing temperature from 15 to ...

journal_title：FEBS letters

authors： Binda F,Bossi E,Giovannardi S,Forlani G,Peres A

更新日期：2002-02-13 00:00:00

abstract：:We present here a new set of aminoglycoside-arginine conjugates (AACs) that are either site-specific or per-arginine conjugates of paromomycin, neamine, and neomycin B as well as their structure-activity relationships. Their binding constants (KD) for TAR and RRE RNAs, measured by fluorescence anisotropy, revealed dep...

journal_title：FEBS letters

authors： Lapidot A,Vijayabaskar V,Litovchick A,Yu J,James TL

更新日期：2004-11-19 00:00:00

abstract：:The homeodomain protein, Cdx2, has been implicated in the transcriptional regulation of genes expressed in the small intestine. In vitro studies of the carbonic anhydrase 1 (CA1) colon promoter implied that Cdx2 may also play a role in the regulation of colon-specific gene expression. The current work follows up this ...

journal_title：FEBS letters

authors： Drummond FJ,Sowden J,Morrison K,Edwards YH

更新日期：1998-02-20 00:00:00

abstract：:We report on the cDNA cloning and characterization of a novel short-chain isoprenyl diphosphate synthase from the aphid Myzus persicae. Of the three IPPS cDNAs we cloned, two yielded prenyltransferase activity following expression in Escherichia coli; these cDNAs encode identical proteins except for the presence, in o...

journal_title：FEBS letters

authors： Vandermoten S,Charloteaux B,Santini S,Sen SE,Béliveau C,Vandenbol M,Francis F,Brasseur R,Cusson M,Haubruge E

更新日期：2008-06-11 00:00:00

abstract：:The main role of voltage-gated proton channels (Hv1) is to extrude protons from the intracellular milieu when, mediated by different cellular processes, the H(+) concentration increases. Hv1 are exquisitely selective for protons and their structure is homologous to the voltage sensing domain (VSD) of other voltage-gat...

journal_title：FEBS letters

authors： Castillo K,Pupo A,Baez-Nieto D,Contreras GF,Morera FJ,Neely A,Latorre R,Gonzalez C

更新日期：2015-11-14 00:00:00

abstract：:Classically, alpha-1,4-glucan synthases have been divided into two families, animal/fungal glycogen synthases (GS) and bacterial/plant starch synthases (G(S)S), according to differences in sequence, sugar donor specificity and regulatory mechanisms. Detailed sequence analysis, predicted secondary structure comparison ...

journal_title：FEBS letters

authors： Cid E,Geremia RA,Guinovart JJ,Ferrer JC

更新日期：2002-09-25 00:00:00

abstract：:Ribonucleoprotein complexes, which contain mRNAs and their regulator proteins, carry out post-transcriptional control of gene expression. The function of many RNA-binding proteins depends on their association with cofactors. Here, we use a genomic approach to identify transcripts associated with DLC-1, a protein previ...

journal_title：FEBS letters

authors： Day NJ,Ellenbecker M,Voronina E

更新日期：2018-11-01 00:00:00

abstract：:A 33-kDa protein component of the oxygen-evolving complex in photosystem II is essential for photosynthesis, and it has been believed that mutants with deletion of this 33-kDa protein are not found in higher plants. We report here the first isolation of an Arabidopsis thaliana mutant with a defect in one of the genes ...

journal_title：FEBS letters

authors： Murakami R,Ifuku K,Takabayashi A,Shikanai T,Endo T,Sato F

更新日期：2002-07-17 00:00:00

abstract：:Laser flash-induced photovoltage responses of the D85S and D85T mutants as well as of the wild-type acid blue form are similar and reflect intraprotein charge redistribution caused by retinal isomerization. The Cl- -induced transition of all of these blue forms into purple ones is accompanied by the appearance of elec...

journal_title：FEBS letters

authors： Kalaidzidis IV,Kaulen AD

更新日期：1997-12-01 00:00:00

abstract：:The organization of the U1snRNP-specific A protein (34 kDa) has been analyzed by 12 and 16 A thiol-reversible chemical cross-linking and Western blotting. A-containing cross-linked complexes had molecular masses of 43, 47, 56, 62, 67, 105 and 125 kDa. None of these complexes could be cross-linked following ribonucleas...

journal_title：FEBS letters

authors： Boix J,Habets WJ,van Venrooij WJ,Smith HC

更新日期：1990-10-15 00:00:00