Abstract:
:Moloney murine leukemia virus reverse transcriptase (RT) selectively uses deoxyribonucleotides over ribonucleotides (rNTPs) as substrates. Substitution of F155 with valine (F155V) was previously found to increase the enzyme's affinity for rNTPs, though without affecting the V(max) for catalysis, and thereby conferred to the enzyme significant RNA polymerase activity. We have sought new mutations that might increase the RNA polymerase activity of the F155V mutant. We report here that substitution of Q84 with alanine improved RT-F155V's RNA polymerase activity, but also its DNA polymerase activity. Kinetic analysis and gel-retardation assays suggested that the substitution increased the enzyme's general affinity for the template-primer.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Liu S,Goff SP,Gao Gdoi
10.1016/j.febslet.2006.01.044keywords:
subject
Has Abstractpub_date
2006-02-20 00:00:00pages
1497-501issue
5eissn
0014-5793issn
1873-3468pii
S0014-5793(06)00097-4journal_volume
580pub_type
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