Abstract:
:Little is known about the mechanisms underlying calcium-induced Ca2+ release (CICR) in plants. The slow-activating vacuolar (SV) channel is both permeable to, and activated by Ca2+, and is therefore a prime candidate for a role in CICR. Cytosol-side-out vacuolar membrane vesicles loaded with 45Ca2+ showed voltage- and Ca(2+)-dependent Ca2+ release, which was sensitive to the SV channel modulators DIDS, protein phosphatase 2B and calmodulin. Significantly, voltage-dependent Ca2+ release strongly depended on cytoplasmic Ca2+ concentrations. The results support the notion that CICR occurs in plant cells and that the process can be catalysed by the SV channel on the vacuolar membrane.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Bewell MA,Maathuis FJ,Allen GJ,Sanders Ddoi
10.1016/s0014-5793(99)01109-6keywords:
subject
Has Abstractpub_date
1999-09-10 00:00:00pages
41-4issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(99)01109-6journal_volume
458pub_type
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