Enhancement by streptozotocin of O2- radical generation by the xanthine oxidase system of pancreatic beta-cells.

Abstract:

:Spin-trapping techniques and electron spin resonance (ESR) spectroscopy were used to study the relationship between the effect of streptozotocin (STZ) on pancreatic beta-cells and free radical formation by these cells. Results showed that STZ enhanced generation of the DMPO-OH radical adduct, which is a degradation product of the superoxide anion (O2-) in the presence of cellular components, in a hypoxanthine-xanthine oxidase (XOD) system with a homogenate of beta-cells. This enhancing effect was also observed in a system without cellular components; STZ increased the signal height due to the O2- radical in a concentration-dependent manner and caused a maximum of 150% enhancement at a concentration of 1.5 mM. Thus, STZ seemed to enhance the generation of the O2- radical in the XOD system, probably by some mechanism of its interaction with XOD. Pancreatic beta-cells exhibited a high XOD activity and a very low superoxide dismutase activity. Therefore, the present result supports the possibility that the cytotoxic effect of STZ is closely related to free radical generation in pancreatic beta-cells.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Nukatsuka M,Sakurai H,Yoshimura Y,Nishida M,Kawada J

doi

10.1016/0014-5793(88)80938-4

subject

Has Abstract

pub_date

1988-11-07 00:00:00

pages

295-8

issue

2

eissn

0014-5793

issn

1873-3468

pii

0014-5793(88)80938-4

journal_volume

239

pub_type

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