Abstract:
:Spin-trapping techniques and electron spin resonance (ESR) spectroscopy were used to study the relationship between the effect of streptozotocin (STZ) on pancreatic beta-cells and free radical formation by these cells. Results showed that STZ enhanced generation of the DMPO-OH radical adduct, which is a degradation product of the superoxide anion (O2-) in the presence of cellular components, in a hypoxanthine-xanthine oxidase (XOD) system with a homogenate of beta-cells. This enhancing effect was also observed in a system without cellular components; STZ increased the signal height due to the O2- radical in a concentration-dependent manner and caused a maximum of 150% enhancement at a concentration of 1.5 mM. Thus, STZ seemed to enhance the generation of the O2- radical in the XOD system, probably by some mechanism of its interaction with XOD. Pancreatic beta-cells exhibited a high XOD activity and a very low superoxide dismutase activity. Therefore, the present result supports the possibility that the cytotoxic effect of STZ is closely related to free radical generation in pancreatic beta-cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Nukatsuka M,Sakurai H,Yoshimura Y,Nishida M,Kawada Jdoi
10.1016/0014-5793(88)80938-4subject
Has Abstractpub_date
1988-11-07 00:00:00pages
295-8issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(88)80938-4journal_volume
239pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The Arabidopsis thalina genome database was searched for homologues of the Candida cloacae fao1 gene which encodes a membrane bound, flavin-containing, hydrogen peroxide generating, long chain alcohol oxidase. This gene has not been isolated from plants or animals. Four putative candidates were found in the database b...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.07.086
更新日期:2004-09-10 00:00:00
abstract::Characterization of lignified Zinnia elegans hypocotyls by both alkaline nitrobenzene oxidation and thioacidolysis reveals that coniferyl alcohol units are mainly found as part of 4-O-linked end groups and aryl-glycerol-beta-aryl ether (beta-O-4) structures. Z. elegans hypocotyls also contain a basic peroxidase (EC 1....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03339-2
更新日期:2002-10-09 00:00:00
abstract::Using an all-atom, molecular dynamics-based, flexible docking method, the tertiary and quaternary structures of protofilaments of the "K3" fragment from beta(2)-microglobulin (residues Ser20-Lys41) were predicted at low pH in a continuous mixture of water and 2,2,2-trifluoroethanol (TFE). Tetramers with energies very ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.10.022
更新日期:2006-11-13 00:00:00
abstract::The phosphatidylinositol 3-kinase/Akt pathway is responsible for key aspects of tumor progression, and is frequently hyperactivated in cancer. We have recently identified palladin, an actin-bundling protein that functions to control the actin cytoskeleton, as an Akt1-specific substrate that inhibits breast cancer cell...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.10.056
更新日期:2010-12-01 00:00:00
abstract::TLR3 and TLR4 utilize adaptor TRIF to activate interferon regulatory factor 3 (IRF3), resulting in interferon β (IFN-β) production to mediate anti-viral infection. In this report, we analyzed the effect of two known phosphatidylinositol 3-kinase (PI3K) inhibitors LY294002 and wortmannin on LPS- and poly(I:C)-induced I...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.01.016
更新日期:2012-03-23 00:00:00
abstract::Ca2+-dependent chromatography of soluble cytosolic proteins on calmodulin-Sepharose gave a fraction that exhibited Ca2+- and calmodulin-dependent phosphorylation of several polypeptides, including 60, 56 and 45 kDa species. At 0.2 microM beef calmodulin the phosphorylation was optimal at 3 microM free Ca2+, and at 80 ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81226-7
更新日期:1987-07-13 00:00:00
abstract::Glycogen synthase from human and bovine polymorphonuclear leukocytes was purified to homogeneity. Rabbit antisera were raised against the two glycogen synthases and used for immunochemical analysis. Western blotting analysis showed that the subunit of glycogen synthase in crude homogenates of human and bovine leukocyt...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80653-7
更新日期:1988-03-28 00:00:00
abstract::For understanding the mechanism(s) relating inflammation to corticosteroid action, the effect of tumour necrosis factor-alpha (TNF-alpha) on 11beta-hydroxysteroid dehydrogenase type 2 (11beta-HSD2), the enzyme regulating access of 11beta-hydroxycorticosteroids to receptors, was studied in LLC-PK(1) cells. We observed ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03004-6
更新日期:2001-11-02 00:00:00
abstract::Members of the connexin family differ most in their carboxy-termini, both with respect to sequence and length. In order to assess the contribution of this region to channel function, a series of carboxy-terminal deletion mutants were tested in the paired-oocyte expression system. Connexin-32 can be truncated by 64 ami...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00819-1
更新日期:1994-08-29 00:00:00
abstract::Because phosphorylation of protein kinase C (PKC) may provide a mechanism for regulation of this enzyme, we have examined the ability of two other kinases to phosphorylate PKC. Our results show that casein kinase 1 (CK-1), but not casein kinase 2 (CK-2), can phosphorylate PKC in the absence of Ca2+ and phospholipids. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81092-0
更新日期:1989-09-11 00:00:00
abstract::In photosystem II (PSII) the probability that energy absorbed by core antenna chlorophyll (Chl) is transferred to the reaction center (RC) is extremely high. Although close proximity between antenna Chl ensures a high transfer efficiency, relative pigment orientation can fractionally modify it. This level of refinemen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/S0014-5793(04)00133-4
更新日期:2004-03-12 00:00:00
abstract::Hypoxic-ischaemic encephalopathy, resulting from asphyxia during birth, affects 2-3 in every 1000 term infants and depending on severity, brings about life-changing neurological consequences or death. This hypoxic-ischaemia (HI) results in a delayed neural energy failure during which the majority of brain injury occur...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12943
更新日期:2018-03-01 00:00:00
abstract::Fatty acid synthase (Fasn) is a key component of energy metabolism that is dynamically induced by food intake. Although extensive studies have revealed a number of transcription factors involved in the fasting/refeeding transition of Fasn expression in hepatocytes, much less evidence is available for adipocytes. Using...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12620
更新日期:2017-04-01 00:00:00
abstract::In order to better define the structural elements involved in allosteric signalling, wild-type DnaK and three deletion mutants of the peptide binding domain have been characterized by biophysical (steady-state and time-resolved fluorescence) and biochemical methods. In the presence of ATP the chemical environment of t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03752-3
更新日期:2003-01-02 00:00:00
abstract::Analyzing the primary structure we predicted that calponin may interact with phospholipids. In order to check this suggestion we investigated the interaction of calponin with phospholipids by ultracentrifugation, light scattering, vesicles leakage and differential scanning calorimetry. In agreement with our prediction...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00868-a
更新日期:1995-09-04 00:00:00
abstract::It is known that narcotic antagonists interact with many cholinergic pathways but less in known about specific mechanisms. Using neonatal rat myoballs to study single channel behaviour of the acetylcholinegated nicotinic receptor, it was found that micromolar concentrations of naltrexone had no effect on channel condu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81145-5
更新日期:1985-03-11 00:00:00
abstract::HBV particles with genome derived from spliced mRNAs accumulate in patients with virus-derived severe liver necrosis and fibrosis. We investigated the role of an intronic element (intronic splicing silencer-long, ISS(L)) on splicing of HBV minigene transcripts. Removal of the entire ISS(L) showed two-fold increase in ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.09.026
更新日期:2011-10-20 00:00:00
abstract::The glycogen branching enzyme from Vibrio vulnificus (VvGBE) transfers short side chains (DP 3-5) significantly greater than any other bacterial glycogen branching enzyme (GBE). To elucidate the role of the N-domain of VvGBE in the unique branching pattern, domain-truncated (N1 and N) and N1-domain-swapped (with VvGBE...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.03.011
更新日期:2015-04-28 00:00:00
abstract::CD38, a type II transmembrane glycoprotein, behaves as a catalytically active transporter responsible for ectocellular generation of cyclic ADP-ribose (cADPR) from NAD+ and for subsequent influx of cADPR across membranes [Franco, L., Guida, L., Bruzzone, S., Zocchi, E., Usai, C. and De Flora, A. (1998) FASEB J. in pre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00929-6
更新日期:1998-08-21 00:00:00
abstract::The effect of increasing low density lipoprotein (LDL) concentrations on the synthesis of basement membrane components was investigated in proliferating porcine aortic endothelial cells (PAEC) in culture. Basement membrane-associated heparan sulfate proteoglycan (HSPG) and fibronectin were determined by enzyme immunoa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80758-b
更新日期:1990-05-07 00:00:00
abstract::Particulate drug carriers, including liposomes, are rapidly removed from blood by cells of the reticuloendothelial system (RES) with resulting adverse effects on this important host defense system. In order to overcome this and other major disadvantages of liposomes, we have altered liposome composition in an effort t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80506-9
更新日期:1987-10-19 00:00:00
abstract::Irreversible conversion of the purified zinc-containing 7Fe ferredoxin from the thermoacidophilic archaeon Sulfolobus sp. strain 7 was found to occur under aerobic conditions at pH 5.0 and at 4 degrees C. This process accompanied a substantial increase of the electron paramagnetic resonance signal attributed to a [3Fe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01286-6
更新日期:1997-11-10 00:00:00
abstract::A tropomyosin-binding protein (app. Mr 17000) was detected in equine platelet preparations by a gel overlay technique. Its isolation, amino acid and partial sequence analyses have shown it to be histone H2B. As with a similar protein from pig platelet preparations [der Terrossian et al. (1983) FEBS Lett. 152, 202-206]...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81130-2
更新日期:1983-06-27 00:00:00
abstract::The rotary motion in response to ATP hydrolysis of the ring of c subunits of the membrane portion, F(o), of ATP synthase, F(o)F(1), is still under contention. It was studied with EF(o)EF(1) (Escherichia coli) using microvideography with a fluorescent actin filament. To overcome the limited specificity of actin attachm...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01436-8
更新日期:2000-04-21 00:00:00
abstract::We report here the isolation and sequence determination of a gene closely linked to the Astyanax red visual pigment gene. Reverse transcription polymerase chain reaction assays show that this new gene (B23 Af) and the previously characterized red and green visual pigment genes of Astyanax are all expressed in the eye....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81673-n
更新日期:1993-11-08 00:00:00
abstract::The accumulation of protoporphyrin IX (Proto IX) in light-sensitive mutants of Escherichia coli was detected by spectrofluorimetry. Fluorescence emission and excitation spectra were recorded from extracts of bacterial cells. Proto IX clearly accumulated in cells with mutations in the visA (hemH) gene but not in the wi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81341-i
更新日期:1992-10-05 00:00:00
abstract::Alterations in both calcitonin (CT) secretion and plasma calcium were recently described in adult obese Zucker rats. We have investigated the CT biosynthetic activity of thyroid glands in 30-day-old obese Zucker rats (fa/fa), and their controls (Lean). Plasma calcium level was significantly increased (+0.6 mg/dl) in o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81083-2
更新日期:1984-08-20 00:00:00
abstract::We synthesized the zwitterionic amphiphile cholesterylphosphorylethylpyridinium. This substance activated protein kinase C (PKC) in a micelle-based assay, but in a vesicle assay it was inhibitory. An analog of this compound, in which the pyridine ring is saturated and the nitrogen methylated, showed similar behaviour ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80629-u
更新日期:1992-06-15 00:00:00
abstract::A conformational transition between E2 and E1 forms of Na, K-ATPase induced by different nucleotides has been studied under steady state conditions using the enzyme labelled with 5-iodoacetamidofluorescein. In the presence of K+ the plot of fluorescence as a function of [ATP], [ADP] or [CTP] (in a range of 5 microM-12...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81510-u
更新日期:1990-06-18 00:00:00
abstract::Quiescent fibroblasts derived from human fetal lung can be stimulated to reinitiate DNA synthesis by sequential addition of 3 nM IGF-1 and a low concentration (8 pM) of EGF or by continuous exposure to 10% fetal calf serum or 10 ng/ml PDGF. Bradykinin blocks the IGF-1 and EGF-dependent signals without affecting the re...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81459-0
更新日期:1989-07-17 00:00:00