VCP/Cdc48 rescues the growth defect of a GPI10 mutant in yeast.

abstract：:Structural differences between platelet and endothelial cell thrombospondin (TBSP) were found in two protease-resistant domains (70 and 18 kDa). The 70 kDa fragment is involved in the binding of TBSP to fibrinogen and the 18 kDa fragment in the attachment to various cultured cells. Despite these structural differences...

journal_title：FEBS letters

authors： Clezardin P,Bourdillon MC,Hunter NR,McGregor JL

更新日期：1988-02-15 00:00:00

abstract：:Azide-binding to the heme-copper binuclear center of bo-type ubiquinol oxidase from Escherichia coli was investigated with Fourier-transform infrared spectroscopy. Deconvolution analyses of infrared spectra of the azide (14N3)-inhibited air-oxidized form showed a major infrared azide antisymmetric stretching band at 2...

journal_title：FEBS letters

authors： Tsubaki M,Mogi T,Hori H

更新日期：1999-04-23 00:00:00

abstract：:Using sensitive methods of sequence analysis including hydrophobic cluster analysis, we report here a hitherto undescribed family of modules, the BAH (bromo-adjacent homology) family, which includes proteins such as eukaryotic DNA (cytosine-5) methyltransferases, the origin recognition complex 1 (Orc1) proteins, as we...

journal_title：FEBS letters

authors： Callebaut I,Courvalin JC,Mornon JP

更新日期：1999-03-05 00:00:00

abstract：:Using an all-atom, molecular dynamics-based, flexible docking method, the tertiary and quaternary structures of protofilaments of the "K3" fragment from beta(2)-microglobulin (residues Ser20-Lys41) were predicted at low pH in a continuous mixture of water and 2,2,2-trifluoroethanol (TFE). Tetramers with energies very ...

journal_title：FEBS letters

authors： Standley DM,Yonezawa Y,Goto Y,Nakamura H

更新日期：2006-11-13 00:00:00

abstract：:Poly-ADP ribosylation of nuclear proteins is activated when poly(ADP-ribose) polymerase (PARP), a nuclear zinc-finger enzyme, binds to single-strand DNA breaks. To understand how the signal emerging from its DNA-binding domain (DBD) bound to such breaks is transduced to its catalytic domain, the structure-function rel...

journal_title：FEBS letters

authors： Trucco C,Flatter E,Fribourg S,de Murcia G,Ménissier-de Murcia J

更新日期：1996-12-16 00:00:00

abstract：:The possible role of sphingomyelin cycle for the regulation of cell proliferation was investigated in human keratinocytes. The time-dependent breakdown of sphingomyelin was observed in the immortalized human keratinocyte cell line HaCaT as well as in primary human keratinocytes thereby providing evidence that the sphi...

journal_title：FEBS letters

authors： Geilen CC,Bektas M,Wieder T,Orfanos CE

更新日期：1996-01-02 00:00:00

abstract：:Apoptosis repressor with caspase recruitment domain is expressed at high levels in brain and myogenic tissues, consistent with a role to inhibit apoptosis in the terminally differentiated cells. Expression of ARC in cancers is not known. In this study, we reported that ARC was highly expressed in various non-myogenic ...

journal_title：FEBS letters

authors： Wang M,Qanungo S,Crow MT,Watanabe M,Nieminen AL

更新日期：2005-04-25 00:00:00

abstract：:Myeloperoxidase (MPO) is the most abundant protein in neutrophils and plays a central role in microbial killing and inflammatory tissue damage. Because most of the non-steroidal anti-inflammatory drugs and other drugs contain a thiol group, it is necessary to understand how these substrates are oxidized by MPO. We hav...

journal_title：FEBS letters

authors： Burner U,Jantschko W,Obinger C

更新日期：1999-01-29 00:00:00

abstract：:The pluripotent nature of human embryonic stem cells (hESC) has attracted great interest in using them as a source of cells or tissue in cell therapy. However, in order to be used in regenerative medicine, the pluripotent hESC lines should be established and propagated according to good manufacturing practice quality ...

journal_title：FEBS letters

authors： Skottman H,Dilber MS,Hovatta O

更新日期：2006-05-22 00:00:00

abstract：:We have shown previously that the skeletal muscle ryanodine receptor mRNA of approximately 16,000 nucleotides codes 5,037 amino acid residues constituting the calcium release channel in skeletal muscle. In this study, RNA blot hybridization analysis shows that the brain contains an RNA species with an estimated size o...

journal_title：FEBS letters

authors： Takeshima H,Nishimura S,Nishi M,Ikeda M,Sugimoto T

更新日期：1993-05-10 00:00:00

abstract：:We identified a gene encoding a novel secreted protein in mice, humans, and zebrafish. As the protein of 222 amino acids is similar to Brorin, a secreted BMP antagonist, which is a member of the Chordin family, we named it Brorin-like. Recombinant Brorin-like protein weakly but significantly inhibited the activity of ...

journal_title：FEBS letters

authors： Miwa H,Miyake A,Kouta Y,Shimada A,Yamashita Y,Nakayama Y,Yamauchi H,Konishi M,Itoh N

更新日期：2009-11-19 00:00:00

abstract：:The changes in phosphoproteins purified with the affinity peptide p9CKShs1 were analyzed from extracts of regenerating rat livers in order to define some G1 and G1/S regulations characteristic of mature hepatocytes stimulated to proliferate. We observed a 47 kDa phosphoprotein that occurred first at the end of G1 befo...

journal_title：FEBS letters

authors： Bilodeau M,Talarmin H,Ilyin G,Rescan C,Glaise D,Cariou S,Loyer P,Guguen-Guillouzo C,Baffet G

更新日期：1999-06-11 00:00:00

abstract：:The impact of hexose-6-phosphate dehydrogenase (H6PDH) on 11beta-hydroxysteroid dehydrogenase (11beta-HSD) type 1 activity was investigated upon coexpression in HEK-293 cells. Confocal microscopy analysis indicated colocalisation of both enzymes at the lumenal side of the endoplasmic reticulum (ER) membrane. Functiona...

journal_title：FEBS letters

authors： Atanasov AG,Nashev LG,Schweizer RA,Frick C,Odermatt A

更新日期：2004-07-30 00:00:00

abstract：:The functional molecular masses of the vacuolar membrane H+-ATPase in Saccharomyces cerevisiae under two kinetic conditions for ATP hydrolysis were measured by radiation inactivation. When vacuolar membrane vesicles were exposed to gamma-rays from 60Co, the activities catalyzing a single-cycle and multi-cycles of ATP ...

journal_title：FEBS letters

authors： Hirata R,Ohsumi Y,Anraku Y

更新日期：1989-02-27 00:00:00

abstract：:Characterization of lignified Zinnia elegans hypocotyls by both alkaline nitrobenzene oxidation and thioacidolysis reveals that coniferyl alcohol units are mainly found as part of 4-O-linked end groups and aryl-glycerol-beta-aryl ether (beta-O-4) structures. Z. elegans hypocotyls also contain a basic peroxidase (EC 1....

journal_title：FEBS letters

authors： Pomar F,Caballero N,Pedreño M,Ros Barceló A

更新日期：2002-10-09 00:00:00

abstract：:Dynein is a minus end-directed microtubule motor that serves multiple cellular functions. We have performed a fine mapping of the 8 kDa dynein light chain (LC8) binding sites throughout the development of a library of consecutive synthetic dodecapeptides covering the amino acid sequences of the various proteins known ...

journal_title：FEBS letters

authors： Rodríguez-Crespo I,Yélamos B,Roncal F,Albar JP,Ortiz de Montellano PR,Gavilanes F

更新日期：2001-08-17 00:00:00

abstract：:In the present study, we investigated the roles and molecular mechanisms of miR-320a in human nasopharyngeal carcinoma (NPC). miR-320a expression was strongly reduced in NPC tissues and cell lines. Overexpression of miR-320a significantly suppressed NPC cell growth, migration, invasion and tumor growth in a xenograft ...

journal_title：FEBS letters

authors： Qi X,Li J,Zhou C,Lv C,Tian M

更新日期：2014-10-16 00:00:00

abstract：:HSP73, the constitutive form of heat-shock protein 70, has been implicated in the translocation of preproteins across the mitochondrial membranes, being required for maintaining mitochondrial preproteins in an import competent conformation. Here we report that highly purified mitochondrial outer membranes contain a pr...

journal_title：FEBS letters

authors： Lithgow T,Ryan M,Anderson RL,Høj PB,Hoogenraad NJ

更新日期：1993-10-18 00:00:00

abstract：:Internalization of membrane proteins has been studied for more than three decades without solving all the underlying mechanisms. Our knowledge of the clathrin-coated endocytosis is sufficient to understand the basic principles. However, more detailed insight is required to recognize why different proteins enter clathr...

journal_title：FEBS letters

authors： Strous GJ,Gent J

更新日期：2002-10-02 00:00:00

abstract：:A novel calcium-dependent serine proteinase (CASP) secreted from malignant hamster embryo fibroblast Ni 12C2 degrades extracellular matrix proteins. A complementary DNA encoding CASP has been isolated with the use of oligonucleotide probes synthesized based on partial amino acid sequences of CASP. The complete amino a...

journal_title：FEBS letters

authors： Kinoshita H,Sakiyama H,Tokunaga K,Imajoh-Ohmi S,Hamada Y,Isono K,Sakiyama S

更新日期：1989-07-03 00:00:00

abstract：:The formation of fibrils by amyloid beta-protein (Abeta) is considered as a key step in the pathology of Alzheimer's disease (AD). Inhibiting the aggregation of Abeta is a promising approach for AD therapy. In this study, we used biocompatible nanogels composed of a polysaccharide pullulan backbone with hydrophobic ch...

journal_title：FEBS letters

authors： Ikeda K,Okada T,Sawada S,Akiyoshi K,Matsuzaki K

更新日期：2006-12-11 00:00:00

abstract：:Using top-of-the-line fold recognition methods, we assigned an oligonucleotide/oligosaccharide-binding (OB)-fold structure to a family of previously uncharacterized hypothetical proteins from several bacterial genomes. This novel family of bacterial OB-fold (BOF) proteins present in a number of pathogenic strains enco...

journal_title：FEBS letters

authors： Ginalski K,Kinch L,Rychlewski L,Grishin NV

更新日期：2004-06-04 00:00:00

abstract：:SV40 large T antigen is phosphorylated at up to ten different amino acids clustered in an N-terminal and a C-terminal part of the polypeptide chain. The N-terminal phosphorylated residues include Ser 123 and Thr 124. We have analyzed the oligomerization, the complex formation with the cellular oncoprotein p53 and the ...

journal_title：FEBS letters

authors： Montenarh M,Müller D

更新日期：1987-09-14 00:00:00

abstract：:The conversion of the cellular prion protein into the beta-sheet-rich scrapie prion protein is thought to be the key step in the pathogenesis of prion diseases. To gain insight into this structural conversion, we analyzed the intrinsic structural propensity of the amino acid sequence of the murine prion C-terminal dom...

journal_title：FEBS letters

authors： Jamin N,Coïc YM,Landon C,Ovtracht L,Baleux F,Neumann JM,Sanson A

更新日期：2002-10-09 00:00:00

abstract：:Lipid metabolism is extensively studied in Saccharomyces cerevisiae. Here, we report that revertant of glycogen synthase kinase mutation-1 (Rog1p) possesses monoacylglycerol (MAG) lipase activity in S. cerevisiae. The lipase activity of Rog1p was confirmed in two ways: through analysis of a strain with a double deleti...

journal_title：FEBS letters

authors： Vishnu Varthini L,Selvaraju K,Srinivasan M,Nachiappan V

更新日期：2015-01-02 00:00:00

abstract：:In yeast, three AAA superfamily metalloproteases (Yme1p, Afg3p and Rca1p) are localized to the mitochondrial inner membrane where they perform roles in the assembly and turnover of the respiratory chain complexes. We have investigated the function of the proposed human orthologue of yeast Yme1p, encoded by the YME1L g...

journal_title：FEBS letters

authors： Shah ZH,Hakkaart GA,Arku B,de Jong L,van der Spek H,Grivell LA,Jacobs HT

更新日期：2000-08-04 00:00:00

abstract：:SV40 large tumor-antigen (T-ag) nuclear import is enhanced by the protein kinase CK2 (CK2) site (Ser111Ser112) flanking the nuclear localization sequence (NLS). Here we use site-directed mutagenesis to examine the influence of negative charge and conformation at the site on T-ag nuclear import and recognition by the N...

journal_title：FEBS letters

authors： Xiao CY,Jans P,Jans DA

更新日期：1998-12-04 00:00:00

abstract：:To complete the amino acid sequence of GTP:AMP phosphotransferase (MgGTP + AMP in equilibrium with MgGDP + ADP) from beef heart mitochondria it was necessary to sequence an intermediate region of about 33 residues after position 102 [(1984) Eur. J. Biochem. 143, 331-339] and find a suitable overlap with the rest of th...

journal_title：FEBS letters

authors： Tomasselli AG,Frank R,Schiltz E

更新日期：1986-07-07 00:00:00

abstract：:Silent information regulator 2 (Sir2) is an NAD(+)-dependent histone deacetylase that establishes repressive chromatin status and extends the life span of both budding yeast and the nematode worm Caenorhabditis elegans. There is growing evidence that its mammalian homologue Sir2alpha protects cells from stress-induced...

journal_title：FEBS letters

authors： Ohsawa S,Miura M

更新日期：2006-10-30 00:00:00

abstract：:We present evidence that the potent chloride channel blocker NPPB has protonophoric activity in the mitochondria and across the plasma membrane of phagocytic cells. The resting O2 consumption of murine peritoneal macrophages was stimulated up to 2.5-fold in the presence of NPPB, with a K0.5 of 15 microM. The stimulato...

journal_title：FEBS letters

authors： Lukacs GL,Nanda A,Rotstein OD,Grinstein S

更新日期：1991-08-19 00:00:00