Abstract:
:Azide-binding to the heme-copper binuclear center of bo-type ubiquinol oxidase from Escherichia coli was investigated with Fourier-transform infrared spectroscopy. Deconvolution analyses of infrared spectra of the azide (14N3)-inhibited air-oxidized form showed a major infrared azide antisymmetric stretching band at 2041 cm(-1). An additional band developed at 2062.5 cm(-1) during a longer incubation. Isotope substitutions with terminally 15N-labelled azides did not show a splitting of the major band, indicating that the geometry of the bound azide is mainly in a bridging configuration between high-spin heme o and CuB. The band at 2062.5 cm(-1) showed clear splittings upon substitution with the terminally 15N-labelled azides, indicating the Cu(2+)B-N=N=N structure. Partial reduction of the oxidase with beta-NADH in the presence of azide caused an appearance of new infrared bands at 2038.5 (major) and 2009 (minor) cm(-1). The former band also showed clear splittings in the presence of the terminally 15N-labelled azides, indicating that reduction of low-spin heme b alters the structure of the binuclear center leading to the Fe(3+)o-N=N=N configuration.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tsubaki M,Mogi T,Hori Hdoi
10.1016/s0014-5793(99)00423-8keywords:
subject
Has Abstractpub_date
1999-04-23 00:00:00pages
191-5issue
2-3eissn
0014-5793issn
1873-3468pii
S0014-5793(99)00423-8journal_volume
449pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Human synovium was analyzed for the possible expression of cartilage oligomeric matrix protein (COMP). Immunostaining with polyclonal antiserum to COMP demonstrated positive staining within the synovial cells and immediately subjacent connective tissue, with less intense staining in the deeper connective tissue. Weste...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00789-8
更新日期:1997-07-21 00:00:00
abstract::Cathelicidins are a group of myeloid antimicrobial peptide precursors found in a variety of mammalian species. Transcripts of this family show a highly conserved 5' region corresponding to the 5' untranslated region, signal peptide and propiece, and diverse 3' regions encoding structurally varied C-terminal sequences ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01310-0
更新日期:1997-11-17 00:00:00
abstract::The off rate from the first calcium-binding site of the C2A domain of synaptotagmin I, a putative calcium receptor in neurotransmitter release, has been determined by 15N-nuclear magnetic resonance relaxation dispersion measurements. The exchange rate was obtained by fitting the dependence of the transverse relaxation...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02508-5
更新日期:2002-04-10 00:00:00
abstract::We studied the molecular binding specificity of two rabbit polyclonal sera generated against phenolic glycolipid antigens namely PheG1 B and PheG1 B-3 from Mycobacterium bovis BCG. PheG1 B is the well-known mycoside B (2-O-Me-alpha-L-Rhap 1----aglycone), while PheG1 B-3 is a recently found glycolipid (alpha-L-Rhap-(1-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80469-w
更新日期:1992-05-25 00:00:00
abstract::Disulfide bridges were introduced into CrylAa, a Bacillus thuringiensis lepidopteran toxin, to stabilize different protein domains including domain I alpha-helical regions thought to be involved in membrane integration and permeation. Bridged mutants could not form functional ion channels in lipid bilayers in the oxid...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00626-1
更新日期:1997-06-30 00:00:00
abstract::In this paper we introduce monobromobimane, a thiol reagent, as a selective blocker of the recently identified dithiol whose oxidation-reduction status modifies voltage sensing by the mitochondrial permeability transition pore, a cyclosporin A-sensitive channel. Monobromobimane does not inhibit the phosphate carrier, ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00256-9
更新日期:1995-04-03 00:00:00
abstract::Adenosine kinase is one of the enzymes potentially responsible for the formation of cytokinin nucleotides in plants. Using a zeatin affinity column a 40 kDa protein was isolated from tobacco Bright Yellow 2 (TBY-2) and identified by mass spectrometry as adenosine kinase. The ligand interaction reported here can be dis...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03750-x
更新日期:2003-01-02 00:00:00
abstract::The scorpion toxin peptide BeKm-1 was synthesised by fluorenylmethoxycarbonyl solid phase chemistry and folded by air oxidation. The peptide's effects on heterologous human ether-a-go-go-related gene potassium current (I(HERG)) in HEK293 cells were assessed using 'whole-cell' patch clamp. Blockade of I(HERG) by BeKm-1...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00662-8
更新日期:2003-07-17 00:00:00
abstract::The cAMP-dependent induction of 2,5-oligoadenylate (2-5A) synthetase and cAMP-dependent inhibition of 2-5A phosphodiesterase are shown. Variations in activities of cAMP-dependent protein kinase and the enzymes of 2-5A metabolism in the cells deepening into the resting state were found to be compatible with the above f...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80467-6
更新日期:1984-06-04 00:00:00
abstract::Cyclic mononucleotides are messengers in plant stress responses. Here we show that hydrogen peroxide (H2O2) induces rapid net K(+)-efflux and Ca(2+)-influx in Arabidopsis roots. Pre-treatment with either 10μM cAMP or cGMP for 1 or 24h does significantly reduce net K(+)-leakage and Ca(2+)-influx, and in the case of the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.01.062
更新日期:2014-03-18 00:00:00
abstract::Actin filaments copolymerized with both intact and chemically modified actin monomers restored their sliding activity when they were supplemented with tropomyosin extracted from skeletal muscle. In contrast, the ATPase activation of the copolymers was decreased when supplemented with tropomyosin. The results indicate ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00218-9
更新日期:1996-03-25 00:00:00
abstract::Thrombin is a potent activator of human glomerular epithelial cells (HGEC). Here we compare short-term and long-term effects of thrombin and thrombin receptor agonist peptide (TRAP) which selectively activates the functional thrombin receptor. TRAP, as thrombin, increases intracellular free Ca2+ concentration and acts...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81716-d
更新日期:1993-11-15 00:00:00
abstract::We report on the cDNA cloning and characterization of a novel short-chain isoprenyl diphosphate synthase from the aphid Myzus persicae. Of the three IPPS cDNAs we cloned, two yielded prenyltransferase activity following expression in Escherichia coli; these cDNAs encode identical proteins except for the presence, in o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.04.043
更新日期:2008-06-11 00:00:00
abstract::The oxidation of free methionine to methionine sulfoxide by chemically or enzymatically generated oxygen free radicals is presented. The physiological significance of this process in living cells is suggested. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80443-x
更新日期:1987-11-16 00:00:00
abstract::Designed mutations within the Streptomyces lividans kcsA gene resulted in a set of mutant proteins, which were characterized in respect to their assembly and channel activities. (i) The amino acid residue leucine 81 located at the external side of KcsA was found to be exchangeable by a cysteine residue without affecti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01429-0
更新日期:2000-04-21 00:00:00
abstract::Immunoblotting analysis was used to detect the microtubule-associated proteins present at different stages of rat brain development. Polyclonal antibodies were raised against the two main adult brain microtubule-associated proteins: MAP-2 (300 kDa) and TAU (60-70 kDa). Whatever the stage of development, anti-MAP-2 ser...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80397-5
更新日期:1985-09-02 00:00:00
abstract::The phosphatidylinositol 4,5-bisphosphate (PtdIns-P2)-regulated actin-binding protein gelsolin and most phosphoinositide-specific phospholipases C (PLCs) comprise a basic amino acid motif ((K/R)xxxKxK(K/R); x denotes any amino acid) which was previously suggested to represent a PtdInsP2-binding site commonly present i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00464-k
更新日期:1995-05-29 00:00:00
abstract::The amino acid sequence of rat ribosomal protein L26 was deduced from the sequence of nucleotides in a recombinant cDNA and confirmed from the NH2-terminal amino acid sequence of the protein. Rat L26 contains 145 amino acids and has a molecular mass of 17,266 Da. Hybridization of the cDNA to digests of nuclear DNA sug...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81434-6
更新日期:1989-07-17 00:00:00
abstract::The mechanism underlying lipopolysaccharide (LPS)-induced depression of phosphoinositide (PI) hydrolysis was investigated using rat aortas. In LPS-pretreated aortas, the 5-hydroxytryptamine-stimulated accumulation of inositol monophosphate and incorporation of exogenous myo-inositol into PIs were significantly less th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02747-3
更新日期:2002-05-22 00:00:00
abstract::The ∆Np63 isoform of the p53-family transcription factor Trp63 is a key regulator of mammary epithelial stem cells that is involved in breast cancer development. To investigate the role of ∆Np63 at different stages of normal mammary gland development, we generated a ∆Np63-inducible conditional knockout (cKO) mouse mod...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13703
更新日期:2020-03-01 00:00:00
abstract::Placental alkaline phosphatase is an inducible enzyme, expressed in HeLaS3 cells, which has been shown to possess protein phosphotyrosine phosphatase activity. Since phosphotyrosine levels are known to increase in actively dividing cells we sought an inverse correlation between PLAP activity and growth rate in HeLaS3 ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80229-n
更新日期:1993-08-30 00:00:00
abstract::Activity of the plant mitochondrial alternative oxidase (AOX) can be regulated by organic acids, notably pyruvate. To date, only two well-conserved cysteine residues have been implicated in this process. We report the functional expression of two AOX isozymes (Sauromatum guttatum Sg-AOX and Arabidopsis thaliana At-AOX...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.10.107
更新日期:2005-01-17 00:00:00
abstract::Annexins are multifunctional intracellular proteins with Ca2+- and phospholipid-binding properties. Their structures consist of four conserved repeat domains that form the core and a diverse N-terminal tail, from which their functional differences may arise. We searched for cellular proteins that interact with the N-t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01643-3
更新日期:1999-01-15 00:00:00
abstract::Cerebellar granule cells differentiated in culture were incubated with ganglioside [3H-Sph]GM1 in order to have it inserted into the plasma membrane and metabolized. Among the formed metabolites radioactive sphingosine and ceramide were identified. [3H]Ceramide started to be measurable after 10 min of incubation (puls...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80193-k
更新日期:1992-03-30 00:00:00
abstract::Five-lipoxygenase-activating protein (FLAP) is usually described as an essential protein to activate the leukotriene (LTs) synthesis via the 5-lipoxygenase pathway. In the enterocyte model HT29 cl.19A cell line, 5-lipoxygenase metabolism was found despite the lack of FLAP expression. Therefore HT29 cl.19A represents a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01196-x
更新日期:1998-10-16 00:00:00
abstract::Mitochondrial uncoupling protein 2 (UCP2) is abundant in developing monocyte/macrophage cells and may affect hematopoiesis by reducing formation of reactive oxygen species. The aims of this study were to further characterize the involvement of UCP2 in hematopoiesis. In situ hybridization in mouse embryos identified UC...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.02.012
更新日期:2007-03-20 00:00:00
abstract::Under physiological conditions significant amounts of low-density lipoprotein LDL particles ar taken up by cells independently of specific high-affinity LDL receptors (apo-B receptors). Previously it was established that some cells contain surface sites capable of binding liposomes. We proposed that liposome-binding s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80006-o
更新日期:1991-08-05 00:00:00
abstract::The recently proposed mechanistic concept of a receptor-regulated entrance compartment for hexose transport formed by microvilli on 3T3-L1 adipocytes predicted a preferential localization of glucose transporters in these structures. The cytochalasin B-binding technique was used to determine in basal and insulin-stimul...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80615-p
更新日期:1990-02-26 00:00:00
abstract::The human mutT homolog, hMTH1, suppresses spontaneous mutations by degrading the endogeneous mutagen, 8-hydroxy-dGTP. We previously reported the broad substrate specificity of hMTH1, which also degrades the oxidatively damaged purine nucleotides, 2-hydroxy-dATP, 8-hydroxy-dATP, 2-hydroxy-ATP, and 8-hydroxy-GTP, in add...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02240-8
更新日期:2002-02-13 00:00:00
abstract::The ability of three fatty acids and their respective ethyl esters, to promote generation of reactive oxygen species (ROS), was compared in a preparation of rat brain synaptosomes. Arachidonic but not palmitic or linoleic acids promoted ROS generation. Ethyl esterification of each fatty acid significantly enhanced ROS...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01173-c
更新日期:1995-11-13 00:00:00