Formation of free sphingosine and ceramide from exogenous ganglioside GM1 by cerebellar granule cells in culture.

abstract：:The neurogenic gene Drosophila big brain (bib) has a high sequence homology to aquaporin-4. However, its cellular functions in Drosophila neurogenesis have remained elusive. Here we investigated cell adhesion, and the ion and water permeability of Bib. The adhesive function was examined by a cell aggregation assay usi...

journal_title：FEBS letters

authors： Tatsumi K,Tsuji S,Miwa H,Morisaku T,Nuriya M,Orihara M,Kaneko K,Okano H,Yasui M

更新日期：2009-06-18 00:00:00

abstract：:Natural freeze tolerance depends on cellular adaptations that address the multiple stresses imposed on cells during freezing. These adaptations preserve viability by suppressing energy-expensive cell processes in the frozen state. In this study, we explore the freeze-responsive expression of microRNA in hatchling pain...

journal_title：FEBS letters

authors： Biggar KK,Storey KB

更新日期：2015-11-30 00:00:00

abstract：:The nucleotide sequence of cDNA encoding the human substance P precursor, beta-preprotachykinin (beta-PPT), has been determined. The source of mRNA was a human laryngeal carcinoid tumour that contained a high concentration of immunoreactive substance P. The human beta-PPT polypeptide is 129 amino acids long and contai...

journal_title：FEBS letters

authors： Harmar AJ,Armstrong A,Pascall JC,Chapman K,Rosie R,Curtis A,Going J,Edwards CR,Fink G

更新日期：1986-11-10 00:00:00

abstract：:WT1 encodes a zinc finger protein with a key role in urogenital development that is inactivated in a subset of Wilms' tumors. This tumor suppressor gene product contains an amino-terminal dimerization domain required for trans-inhibition of wild-type WT1 activity by mutants defective for DNA binding. In the course of ...

journal_title：FEBS letters

authors： Bruening W,Moffett P,Chia S,Heinrich G,Pelletier J

更新日期：1996-09-09 00:00:00

abstract：:Nuclear pore complexes (NPC) form nuclear pores that cross the nuclear envelope and allow molecules to transport between the nucleus and the cytoplasm. We solved the crystal structure of human Nup43 (hNUP43), an important component in the Nup107 subcomplex of NPC. hNup43 adopts a seven-bladed β-propeller fold. We conf...

journal_title：FEBS letters

authors： Xu C,Li Z,He H,Wernimont A,Li Y,Loppnau P,Min J

更新日期：2015-10-24 00:00:00

abstract：:In fission yeast, the only known essential function of Ned8p is the modification of the cullin, Pcu1p, and subsequent Cullin-RING-Ligase (CRL) activation and substrate ubiquitination. We show here that a functional Pcu1p mutant, deleted for its C-terminal autoinhibitory domain, which negates the requirement of neddyla...

journal_title：FEBS letters

authors： Girdwood D,Robertson M,Gordon C

更新日期：2012-05-21 00:00:00

abstract：:Small organic molecules, like Congo red and lacmoid, have been shown to modulate the self-assembly of the amyloid β peptide (Aβ). Here, we show that Aβ forms NMR invisible non-toxic co-aggregates together with lacmoid as well as Congo red. We find that the interaction involves two distinct kinetic processes and at eve...

journal_title：FEBS letters

authors： Abelein A,Lang L,Lendel C,Gräslund A,Danielsson J

更新日期：2012-11-16 00:00:00

abstract：:The presence of additives during X-irradiation of malate synthase led to radioprotective effects against primary and post-irradiation inactivation. Pronounced effects were provided by typical scavengers, sulfhydryl reagents and specific ligands (substrates, products, analogues). The results show that scavenging and sp...

journal_title：FEBS letters

authors： Durchschlag H,Zipper P

更新日期：1988-09-12 00:00:00

abstract：:This study demonstrated that activation of tyrosine kinase of epidermal growth factor receptor (EGFR) induces its association with G protein-coupled receptor kinase 2 (GRK2). Immunoprecipitation experiments showed that EGF stimulation increased GRK2 binding to EGFR complex in HEK293 cells coexpressing EGFR and GRK2. T...

journal_title：FEBS letters

authors： Gao J,Li J,Chen Y,Ma L

更新日期：2005-01-03 00:00:00

abstract：:Proteins implicated as intracellular chloride channels include the intracellular ClC proteins, the bestrophins, the cystic fibrosis transmembrane conductance regulator, the CLICs, and the recently described Golgi pH regulator. This paper examines current hypotheses regarding roles of intracellular chloride channels an...

journal_title：FEBS letters

authors： Edwards JC,Kahl CR

更新日期：2010-05-17 00:00:00

abstract：:Seryl-tRNA synthetases (SerRSs) fall into two distinct evolutionary groups of enzymes, bacterial and methanogenic. These two types of SerRSs display only minimal sequence similarity, primarily within the class II conserved motifs, and possess distinct modes of tRNA(Ser) recognition. In order to determine whether the t...

journal_title：FEBS letters

authors： Ahel D,Slade D,Mocibob M,Söll D,Weygand-Durasevic I

更新日期：2005-08-15 00:00:00

abstract：:Epigenetic silencing mechanisms play an important role in chemoresistance of human cancer. Here we report the upregulated expression of metastasis-associated protein 1 (MTA1), a component of the nucleosome remodeling deacetylation (NuRD) complex, in chemoresistant prostate cancer (PCa). MTA1 knockdown in PC-3 cells in...

journal_title：FEBS letters

authors： Yu L,Su YS,Zhao J,Wang H,Li W

更新日期：2013-08-19 00:00:00

abstract：:Astrocyte-elevated gene-1 (AEG-1) positively regulates tumor progression and metastasis. Here, we document that AEG-1 upregulates transcription of the membrane protein tetraspanin 8 (TSPAN8). Knocking down TSPAN8 in AEG-1-overexpressing human hepatocellular carcinoma (HCC) cells markedly inhibited invasion and migrati...

journal_title：FEBS letters

authors： Akiel MA,Santhekadur PK,Mendoza RG,Siddiq A,Fisher PB,Sarkar D

更新日期：2016-08-01 00:00:00

abstract：:Isoenzymes of creatine kinase (CK, EC 2.7.3.2) in guinea-pig smooth, cardiac and skeletal muscles as well as in brain were analyzed by cellulose acetate electrophoresis and FPLC gel permeation chromatography. In crude tissue extracts of smooth muscles brain type BB-CK and the hybrid form MB-CK were detected, but in en...

journal_title：FEBS letters

authors： Ishida Y,Wyss M,Hemmer W,Wallimann T

更新日期：1991-05-20 00:00:00

abstract：:A colostral proline-rich polypeptide complex (PRP) consisting of over 30 peptides shows beneficial effects in Alzheimer's disease (AD) patients when administered in the form of sublinqual tablets called Colostrinin. The aim of the present studies was to investigate whether nanopeptide fragment of PRP (NP) - one of the...

journal_title：FEBS letters

authors： Janusz M,Woszczyna M,Lisowski M,Kubis A,Macała J,Gotszalk T,Lisowski J

更新日期：2009-01-05 00:00:00

abstract：:The assembly of the Sm-class of uridine-rich small nuclear ribonucleoproteins (U snRNPs), albeit spontaneous in vitro, has recently been shown to be dependent on the aid of a large number of assisting factors in vivo. These factors are organized in two interacting units termed survival motor neuron (SMN)- and protein ...

journal_title：FEBS letters

authors： Neuenkirchen N,Chari A,Fischer U

更新日期：2008-06-18 00:00:00

abstract：:VacA is a major virulence factor of the widespread stomach-dwelling bacterium Helicobacter pylori. It causes cell vacuolation and tissue damage by forming anion-selective, urea-permeable channels in plasma and endosomal membranes. We report that several flavone derivatives and other polyphenols present in vegetables a...

journal_title：FEBS letters

authors： Tombola F,Campello S,De Luca L,Ruggiero P,Del Giudice G,Papini E,Zoratti M

更新日期：2003-05-22 00:00:00

abstract：:Okadaic acid is an inhibitor of the protein Ser/Thr phosphatases PP1 and PP2A, which blocks the activation of extracellular signal-regulated protein kinase 5 (ERK5), a member of the MAP kinase family activated by growth factors and several types of stressors. The blocking of ERK5 activation by okadaic acid was observe...

journal_title：FEBS letters

authors： Garcia L,Garcia F,Llorens F,Unzeta M,Itarte E,Gómez N

更新日期：2002-07-17 00:00:00

abstract：:Five-lipoxygenase-activating protein (FLAP) is usually described as an essential protein to activate the leukotriene (LTs) synthesis via the 5-lipoxygenase pathway. In the enterocyte model HT29 cl.19A cell line, 5-lipoxygenase metabolism was found despite the lack of FLAP expression. Therefore HT29 cl.19A represents a...

journal_title：FEBS letters

authors： Battu S,Beneytout JL,Pairet M,Rigaud M

更新日期：1998-10-16 00:00:00

abstract：:The effect of the three platelet-derived growth factor (PDGF) isoforms AA, AB and BB on the induction of the early growth response genes c-fos, egr-1 and c-myc mRNA in vascular smooth muscle cells from rat was compared with their respective mitogenic potency. The three PDGF isoforms strongly stimulated the induction t...

journal_title：FEBS letters

authors： Sachinidis A,Schulte K,Ko Y,Meyer zu Brickwedde MK,Hoppe V,Hoppe J,Vetter H

更新日期：1993-03-22 00:00:00

abstract：:We studied the effect of point mutation within the putative 11th transmembrane domain (TM11) of the Na(+)/H(+) exchanger NHE1 on the plasma membrane expression. Of the 19 mutants tested, two mutants (Tyr454 or Arg458 replaced by Cys) were retained in the endoplasmic reticulum. Interestingly, Y454C was expressed on the...

journal_title：FEBS letters

authors： Wakabayashi S,Pang T,Su X,Shigekawa M

更新日期：2000-12-29 00:00:00

abstract：:We report that the mitotic inhibitor, vinblastine (VLB), is highly toxic to the malarial parasite, Plasmodium falciparum. In cultures in vitro growth is inhibited by 50% at a VLB level of about 28 nM, and totally abolished at a level of 100 nM. By tests on synchronized cultures we have found that the effect of VLB tak...

journal_title：FEBS letters

authors： Usanga EA,O'Brien E,Luzzato L

更新日期：1986-12-01 00:00:00

abstract：:RNA-binding protein kinase from amphibian oocytes modifies serine and threonine residues in the molecules of substrates and utilizes both ATP and GTP. Low concentrations of heparin inhibit protein kinase. The foregoing suggests that this enzyme is casein kinase II. It is shown that RNA-binding proteins lack active for...

journal_title：FEBS letters

authors： Kandror KV,Stepanov AS

更新日期：1984-05-07 00:00:00

abstract：:The specific mechanism by which the inhibitory guanine nucleotide binding protein (Gi) mediates the inhibition of adenylate cyclase activity is still unclear. The subunit dissociation model, based on studies in purified or reconstituted systems, suggests that the beta gamma subunit, which is dissociated with activatio...

journal_title：FEBS letters

authors： Avissar S,Schreiber G

更新日期：1990-01-15 00:00:00

abstract：:Chronic inflammation associated with tumor necrosis factor (TNF)-alpha and reactive oxygen species (ROS) is the hallmark of tuberculosis. Mycobacterium tuberculosis (MTB) directly stimulates human monocytes to secrete TNF-alpha. We show the augmented expression of TNF-alpha mRNA in MTB-infected monocytes by cellular a...

journal_title：FEBS letters

authors： Hasan N,Yusuf N,Toossi Z,Islam N

更新日期：2006-05-01 00:00:00

abstract：:The L1CAM antibody A10-A3 efficiently reduces tumor growth in a nude mouse model. Here, we describe the crystal structure of the Fab fragment of A10-A3 determined at 2.0 angstrom resolution. The A10-A3 antibody H3 loop reveals a characteristic arrangement of exposed aromatic residues that may play an important role in...

journal_title：FEBS letters

authors： Wei CH,Lee ES,Jeon JY,Heo YS,Kim SJ,Jeon YH,Kim KH,Hong HJ,Ryu SE

更新日期：2011-01-03 00:00:00

abstract：:RNA silencing as a robust host defense mechanism against plant viruses is generally countered by virus-encoded silencing suppressors. This strategy is now increasingly recognized to be used by animal viruses as well. We present here an overview of the common features shared by some of the better studied plant viral si...

journal_title：FEBS letters

authors： Qu F,Morris TJ

更新日期：2005-10-31 00:00:00

abstract：:The NK-lysin derived peptide NK-2 is a potent antibacterial, but non-toxic to a human keratinocyte cell line and of low hemolytic activity. Its target selectivity is based upon a strong binding preference to membranes containing anionic phospholipids, which are normally not found on the surface of human cells. Here, w...

journal_title：FEBS letters

authors： Schröder-Borm H,Bakalova R,Andrä J

更新日期：2005-11-07 00:00:00

abstract：:T cells are stimulated by the engagement of antigen, cytokine, pathogen, and hormone receptors. While research performed over many years has focused on deciphering the molecular components of these pathways, recent data underscore the importance of the metabolic environment in conditioning responses to receptor engage...

journal_title：FEBS letters

authors： Yong CS,Abba Moussa D,Cretenet G,Kinet S,Dardalhon V,Taylor N

更新日期：2017-10-01 00:00:00

abstract：:According to the leading theory, the agent responsible for prion diseases would be the conformational isomer PrP(Sc) of a cellular protein PrP(C), the pathogenic form PrP(Sc) multiplying by converting the normal protein into a likeness of itself. The pathogenic isoform could catalyze the conformational transition so t...

journal_title：FEBS letters

authors： Laurent M

更新日期：1997-04-21 00:00:00