Abstract:
:Cerebellar granule cells differentiated in culture were incubated with ganglioside [3H-Sph]GM1 in order to have it inserted into the plasma membrane and metabolized. Among the formed metabolites radioactive sphingosine and ceramide were identified. [3H]Ceramide started to be measurable after 10 min of incubation (pulse), and [3H]sphingosine after 15 min. Their concentrations increased with pulse time, and, after a 1-hour pulse, with chase time. After a 1-hour pulse with 2 x 10(-6) M [3H-Sph]GM1 followed by a 4-hour chase, the amount of [3H]sphingosine and [3H]ceramide formed were 0.04 and 0.4 pmol/10(6) cells, respectively. Particularly the ability to produce sphingosine was higher in differentiated than in undifferentiated cells. It is concluded that ganglioside turnover contributes to the maintenance of the intracellular levels of free sphingosine and ceramide.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Riboni L,Bassi R,Sonnino S,Tettamanti Gdoi
10.1016/0014-5793(92)80193-kkeywords:
subject
Has Abstractpub_date
1992-03-30 00:00:00pages
188-92issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(92)80193-Kjournal_volume
300pub_type
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