Formation of free sphingosine and ceramide from exogenous ganglioside GM1 by cerebellar granule cells in culture.


:Cerebellar granule cells differentiated in culture were incubated with ganglioside [3H-Sph]GM1 in order to have it inserted into the plasma membrane and metabolized. Among the formed metabolites radioactive sphingosine and ceramide were identified. [3H]Ceramide started to be measurable after 10 min of incubation (pulse), and [3H]sphingosine after 15 min. Their concentrations increased with pulse time, and, after a 1-hour pulse, with chase time. After a 1-hour pulse with 2 x 10(-6) M [3H-Sph]GM1 followed by a 4-hour chase, the amount of [3H]sphingosine and [3H]ceramide formed were 0.04 and 0.4 pmol/10(6) cells, respectively. Particularly the ability to produce sphingosine was higher in differentiated than in undifferentiated cells. It is concluded that ganglioside turnover contributes to the maintenance of the intracellular levels of free sphingosine and ceramide.




FEBS letters


Riboni L,Bassi R,Sonnino S,Tettamanti G





Has Abstract


1992-03-30 00:00:00














  • Iron binding to microsomes and liposomes in relation to lipid peroxidation.

    abstract::The effects of ADP, ATP, citrate and EDTA on iron-dependent microsomal and liposomal lipid peroxidation, and on 59FeCl3 binding to the lipid membranes were measured. The aim was to test if initiation of lipid peroxidation is a site-specific mechanism requiring bound iron. In the absence of chelator, iron was bound to ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vile GF,Winterbourn CC

    更新日期:1987-05-04 00:00:00

  • Increasing cAMP antagonizes hypertrophic response to angiotensin II without affecting Ras and MAP kinase activation in vascular smooth muscle cells.

    abstract::Angiotensin II (Ang II), a potent hypertrophic factor for vascular smooth muscle cells (VSMC), induces activation of the ras proto-oncogene product (Ras) and mitogen-activated protein (MAP) kinases, and tyrosine phosphorylation of a focal adhesion-associated protein, paxillin. Forskolin, a direct activator of adenylat...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Takahashi T,Kawahara Y,Okuda M,Yokoyama M

    更新日期:1996-11-11 00:00:00

  • Induction of oxidative DNA damage in anaerobes.

    abstract::We compared oxidative DNA damage in strictly anaerobic Prevotella melaninogenica, aerotolerant anaerobic Bacteroides fragilis, and facultative anaerobic Salmonella typhimurium after exposure to O2 or H2O2. Using HPLC with electrochemical detection, we measured 8-hydroxydeoxyguanosine (8OHdG) as a damage marker. O2 ind...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Takeuchi T,Nakaya Y,Kato N,Watanabe K,Morimoto K

    更新日期:1999-05-07 00:00:00

  • Mapping the heparin-binding domain of boar spermadhesins.

    abstract::Boar spermadhesins are a group of seminal plasma, heparin-binding proteins which appear to be involved in sperm capacitation and gamete interaction. Using a proteolytic protection assay we have identified regions of AQN-1, AQN-3, PSP-I and AWN which remain attached to a heparin-Sepharose column following in-column dig...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Calvete JJ,Dostàlova Z,Sanz L,Adermann K,Thole HH,Töpfer-Petersen E

    更新日期:1996-02-05 00:00:00

  • Evidence from peptide mapping for a human renin zymogen.

    abstract::Data obtained from peptide mapping of the active and inactive forms of human renin show that there are extensive regions of common sequence in the two forms of the enzyme, and are consistent with the hypothesis that inactive renin is a renin zymogen. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: McIntyre GD,Chaudhuri S,Coggins JR

    更新日期:1984-10-01 00:00:00

  • Purification and characterization of the cystinyl bond cleaving yeast aminopeptidase yscXVI.

    abstract::Aminopeptidase yscXVI was purified from the yeast Saccharomyces cerevisiae. By SDS-PAGE the enzyme has a molecular weight of 45,000 Da, and in chromatofocusing, elution was observed at pH 6.2. The synthetic substrate cystinyl-4-nitroanilide (Km 22.5 microM, Vmax 12.9 mU/mg) is cleaved most efficiently in the pH range ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tisljar U,Wolf DH

    更新日期:1993-05-10 00:00:00

  • Lipoprotein MtsA of MtsABC in Streptococcus pyogenes primarily binds ferrous ion with bicarbonate as a synergistic anion.

    abstract::Lipoprotein MtsA is a critical component of MtsABC responsible for iron binding and transport in the Gram-positive bacterium Streptococcus pyogenes. The present collective experimental data establish that Fe(2+) is the primary binding ion for MtsA under optimal physiologically relevant conditions. The binding affiniti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sun X,Ge R,Chiu JF,Sun H,He QY

    更新日期:2008-04-16 00:00:00

  • Human interferon-gamma lacking 23 COOH-terminal amino acids is biologically active.

    abstract::We constructed five mutated cDNAs encoding human interferon-gamma (IFN-gamma) derivatives lacking 19-23 COOH-terminal residues and expressed them in Escherichia coli. All the derivatives were purified to homogeneity. They showed substantially the same order of antiviral activity in vitro as the intact molecule, and be...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sakaguchi M,Honda S,Ozawa M,Nishimura O

    更新日期:1988-03-28 00:00:00

  • Insertion of MOM22 into the mitochondrial outer membrane strictly depends on surface receptors.

    abstract::Targeting of preproteins to mitochondria and their translocation across the outer membrane are mediated by the mitochondrial receptor complex. This protein complex contains the import receptors MOM19 and MOM72 and the general insertion pore GIP. All seven components of the receptor complex are synthesized in the cytos...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Keil P,Pfanner N

    更新日期:1993-04-26 00:00:00

  • Retromer-mediated endosomal protein sorting: The role of unstructured domains.

    abstract::The retromer complex is a key element of the endosomal protein sorting machinery that is conserved through evolution and has been shown to play a role in diseases such as Alzheimer's disease and Parkinson's disease. Through sorting various membrane proteins (cargo), the function of retromer complex has been linked to ...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Mukadam AS,Seaman MN

    更新日期:2015-09-14 00:00:00

  • Connexin hemichannel induced vascular leak suggests a new paradigm for cancer therapy.

    abstract::It is 40 years since cancer growth was correlated with neovascularisation. Anti-angiogenic drugs remain at the forefront of cancer investigations but progress has been disappointing and unexpected toxicities are emerging. Gap junction channels are implicated in lesion spread following injury, with channel blockers sho...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Zhang J,O'Carroll SJ,Henare K,Ching LM,Ormonde S,Nicholson LF,Danesh-Meyer HV,Green CR

    更新日期:2014-04-17 00:00:00

  • Functional effects of cytoskeletal components on the lateral movement of individual BKCa channels expressed in live COS-7 cell membrane.

    abstract::The lateral diffusion of BK(Ca) channels was previously shown to be highly 'confined' in the COS-7 cell membrane. Here we report that the diffusion coefficient and the confinement area of BK(Ca) channel were significantly increased by the treatment of latrunculin A, an actin-depolymerizing agent, but not by microtubul...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Won S,Lee BC,Park CS

    更新日期:2011-07-21 00:00:00

  • The aminoacceptor stem of the yeast tRNA(Lys) contains determinants of mitochondrial import selectivity.

    abstract::Yeast tRNA(Lys)CUU is nucleus-encoded and is partially imported into the mitochondria. Another lysine isoacceptor, tRNA(Lys)SUU, is also nucleus-encoded but is not imported. These two tRNAs differ in 21 bases. We have previously localised import selectivity determinants in the anticodon arm. By in vitro import of muta...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kazakova HA,Entelis NS,Martin RP,Tarassov IA

    更新日期:1999-01-15 00:00:00

  • Nicking of the tryptophan synthase beta 2-subunit at Glu-296 prevents the conformational change undergone on binding the alpha-subunit.

    abstract::Using a monoclonal antibody as conformational probe it has been shown that the weakly active nicked-beta 2 dimer of tryptophan synthase generated by proteolytic cleavage at Glu-296, does not undergo on association with alpha subunit a conformational change known to occur in intact beta 2 subunit. This alpha induced co...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Linkens HJ,Djavadi-Ohaniance L,Goldberg ME

    更新日期:1993-04-12 00:00:00

  • Endurance training alters alanine and glutamine release from muscle during contractions.

    abstract::The release of alanine and glutamine from perfused muscle of trained and control animals was investigated. Release rates did not differ between trained and control muscle at rest. During contractions in trained muscle, alanine release was higher than at rest, while glutamine release was transiently increased. Phenylal...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hood DA,Terjung RL

    更新日期:1994-03-07 00:00:00

  • The formyl peptide chemoattractant receptor is encoded by a 2 kilobase messenger RNA. Expression in Xenopus oocytes.

    abstract::Activation of the formyl peptide chemoattractant receptor (FPCR) of phagocytic cells mobilizes intracellular calcium stores and affects the plasma membrane potential. Affinity crosslinking of FPCR has demonstrated a 60-80 kDa glycoprotein, with core peptide of 32 kDa. It is not known whether functional FPCR is this si...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Murphy PM,Gallin EK,Tiffany HL,Malech HL

    更新日期:1990-02-26 00:00:00

  • Linkage between phosphorylated cystatin alpha and filaggrin by epidermal transglutaminase as a model of cornified envelope and inhibition of cathepsin L activity by cornified envelope and the conjugated cystatin alpha.

    abstract::Lysine-rich phosphorylated cystatin alpha (P-cystatin alpha) from newborn rat epidermis is a good substrate for epidermal transglutaminase (TGase) and also one of the component proteins of cornified envelope in the stratum corneum. Since the filaggrin linker segment peptide was efficiently conjugated with P-cystatin a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Takahashi M,Tezuka T,Kakegawa H,Katunuma N

    更新日期:1994-03-07 00:00:00

  • Two molecules of cytochrome c function as the electron donors to P840 in the reaction center complex isolated from a green sulfur bacterium, Chlorobium tepidum.

    abstract::A photoactive reaction center complex was isolated from a thermophilic green sulfur bacterium, Chlorobium tepidum under anaerobic conditions. The electron transfer occurred from heme c to the photo-oxidized reaction center chlorophyll, P840+, with a half time (t1/2) of 110 or 340 microseconds at 24 or 12 degrees C, re...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Oh-oka H,Kamei S,Matsubara H,Iwaki M,Itoh S

    更新日期:1995-05-22 00:00:00

  • Low-temperature microRNA expression in the painted turtle, Chrysemys picta during freezing stress.

    abstract::Natural freeze tolerance depends on cellular adaptations that address the multiple stresses imposed on cells during freezing. These adaptations preserve viability by suppressing energy-expensive cell processes in the frozen state. In this study, we explore the freeze-responsive expression of microRNA in hatchling pain...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Biggar KK,Storey KB

    更新日期:2015-11-30 00:00:00

  • TNF-alpha enhances intracellular glucocorticoid availability.

    abstract::For understanding the mechanism(s) relating inflammation to corticosteroid action, the effect of tumour necrosis factor-alpha (TNF-alpha) on 11beta-hydroxysteroid dehydrogenase type 2 (11beta-HSD2), the enzyme regulating access of 11beta-hydroxycorticosteroids to receptors, was studied in LLC-PK(1) cells. We observed ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Heiniger CD,Rochat MK,Frey FJ,Frey BM

    更新日期:2001-11-02 00:00:00

  • Secretion of 5-hydroxytryptamine from electropermeabilised human platelets. Effects of GTP and cyclic 3',5'-AMP.

    abstract::Enhancement by thrombin of Ca2+-dependent 5HT secretion in the absence of added GTP decreases as the time between electropermeabilisation and addition of thrombin is increased. No decrease occurs if thrombin is added with GTP. Observation of apparent GTP-independent receptor/phospholipase C coupling may result from th...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Knight DE,Scrutton MC

    更新日期:1987-10-19 00:00:00

  • The roles of organic anion permeases in aluminium resistance and mineral nutrition.

    abstract::Soluble aluminium (Al(3+)) is the major constraint to plant growth on acid soils. Plants have evolved mechanisms to tolerate Al(3+) and one type of mechanism relies on the efflux of organic anions that protect roots by chelating the Al(3+). Al(3+) resistance genes of several species have now been isolated and found to...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Delhaize E,Gruber BD,Ryan PR

    更新日期:2007-05-25 00:00:00

  • Shift in rat liver glycolysis control from fed to starved conditions. Flux control coefficients of glucokinase and phosphofructokinase.

    abstract::The distribution of flux control coefficients of a glycolytic system in starved rat liver has been determined. The flux control coefficient profile in starved conditions is compared with normal fed conditions showing that in the former phosphofructokinase enhances more than 2-fold its flux control coefficient while gl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Torres NV,Mateo F,Meléndez-Hevia E

    更新日期:1988-06-06 00:00:00

  • Beyond the epithelium: cadherin function in fibrous connective tissues.

    abstract::In fibrous connective tissues, fibroblasts are organized into syncytia, cellular networks that enable matrix remodeling and that are interconnected by intercellular adherens junctions (AJs). The AJs of fibroblasts are mediated by N-cadherin, a broadly expressed classical cadherin that is critically involved in develop...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: El Sayegh TY,Kapus A,McCulloch CA

    更新日期:2007-01-23 00:00:00

  • Identification and characterization of the hemoglobin-binding domain of hemoglobin receptor in Leishmania.

    abstract::Leishmania internalize hemoglobin (Hb) via a specific receptor (HbR) for their survival. To identify the Hb-binding domain of HbR, we cloned and expressed several truncated proteins of HbR and determined their ability to bind Hb. Our findings reveal that 90% of Hb-binding activity is retained in HbR41-80 in comparison...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rastogi R,Verma JK,Singh V,Krishnamurthy G,Sood C,Kapoor A,Kumar K,Ansari I,Mukhopadhyay A

    更新日期:2020-12-14 00:00:00

  • Conformational change of 50 S ribosomes on enzymatic binding of phenylalanyl-tRNA.

    abstract::Tight couple 70 S ribosomes are converted to loose couple ones on enzymatic binding of phenylalanyl-tRNA. Enzymatic binding at 0 degree C as well as nonenzymatic binding does not lead to any change. Further, no change takes place when the P site is occupied by N-acetylphenylalanyl-tRNA. Loose couple 70 S ribosomes are...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Srivastava S,Burma DP

    更新日期:1985-07-01 00:00:00

  • Deletion in the Z-line region of the titin gene in a baby hamster kidney cell line, BHK-21-Bi.

    abstract::The gene for titin, a 4MDa myofibrillar protein, was analysed in golden hamster DNAs from different sources, using human cDNA probes and PCR. In the DNA from the BHK-21-Bi subline of baby hamster kidney cells, extended sequences coding for Z-line associated domains were missing, indicating a deletion that renders titi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Jäckel M,Witt C,Antonova O,Curdt I,Labeit S,Jockusch H

    更新日期:1997-05-12 00:00:00

  • Platelet activating factor (PAF-acether) promotes an early degradation of phosphatidylinositol-4,5-biphosphate in rabbit platelets.

    abstract::32P-Labelled washed rabbit platelets were incubated with 0.6 nM platelet activating factor (PAF-acether), giving a full aggregation and release response within 30-60 s. The major phospholipid changes observed under these conditions were: (1) An increased labelling of phosphatidic acid (PA) within 10 s and of phosphati...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mauco G,Chap H,Douste-Blazy L

    更新日期:1983-03-21 00:00:00

  • A pre-existing protease is a common effector of thymocyte apoptosis mediated by diverse stimuli.

    abstract::Data from a number of model systems support a role for proteolysis in apoptotic cell death. Using immature rat thymocytes, we demonstrate that the inhibitors N-tosyl-L-lysyl chloromethylketone (TLCK) and N-tosyl-L-phenylalanyl chloromethylketone (TPCK) have very different effects on apoptosis. TLCK inhibits apoptosis ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fearnhead HO,Rivett AJ,Dinsdale D,Cohen GM

    更新日期:1995-01-09 00:00:00

  • Hetero-oligomerization of adenosine A1 receptors with P2Y1 receptors in rat brains.

    abstract::Adenosine and ATP modulate cellular and tissue functions via specific P1 and P2 receptors, respectively. Although, in general, adenosine inhibits excitability and ATP functions as an excitatory transmitter in the central nervous system, little is known about the direct interaction between P1 and P2 receptors. We recen...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yoshioka K,Hosoda R,Kuroda Y,Nakata H

    更新日期:2002-11-06 00:00:00