Abstract:
:Investigations of the cleavage specificity of a cyclizing endoribonuclease of yeast show that it hydrolyzes Y-A bonds of single-stranded regions of RNA and that oligonucleotides are hydrolyzed poorly. The stringent specificity of the enzyme suggests that it may be useful for generating specific fragments of polyribonucleotides. An isolation technique employing ethanol extraction has now been used to isolate the enzyme free of other RNases, and the studies show that it is a small protein, 10 kDa or less in molecular mass.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Stevens Adoi
10.1016/0014-5793(86)80899-7subject
Has Abstractpub_date
1986-09-15 00:00:00pages
210-4issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(86)80899-7journal_volume
205pub_type
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