Leptospiral TlyC is an extracellular matrix-binding protein and does not present hemolysin activity.

abstract：:Many genes are regulated by the intracellular calcium, protein kinase C (PKC) and protein kinase A (PKA) pathways and it has been shown that these pathways synergize in some cell types, whereas they antagonize in others. Here we show that the calcium and PKC pathways suppress the effects mediated by the PKA pathway in...

journal_title：FEBS letters

authors： Döbbeling U,Berchtold MW

更新日期：1996-08-05 00:00:00

abstract：:The presence of various antigens in two types of isolated endocrine vesicles (chromaffin granules and secretory vesicles of thyroid parafollicular cells) was investigated by immunoblotting. The two types of vesicles have three common secretory proteins: chromogranin A, chromogranin B and secretogranin II. Furthermore,...

journal_title：FEBS letters

authors： Weiler R,Cidon S,Gershon MD,Tamir H,Hogue-Angeletti R,Winkler H

更新日期：1989-11-06 00:00:00

abstract：:Phosphorylation of the 20 kDa myosin light chain from smooth muscle by five different kinases was investigated. Three of the kinases (myosin light chain kinase, phosphorylase kinase, and cAMP-dependent protein kinase) phosphorylate serine residues, the fourth (casein-kinase-2) mainly threonine, and the fifth (glycogen...

journal_title：FEBS letters

authors： Singh TJ,Akatsuka A,Huang KP

更新日期：1983-08-08 00:00:00

abstract：:The proteolysis of beta-amyloid (Abeta) requires neprylisin, an enzyme that has been shown as reduced in Alzheimer's disease (AD). We investigated whether a decrease in neprilysin levels contributes to the accumulation of amyloid deposits not only in AD but also in the normal aging. We analyzed neprilysin mRNA and pro...

journal_title：FEBS letters

authors： Russo R,Borghi R,Markesbery W,Tabaton M,Piccini A

更新日期：2005-11-07 00:00:00

abstract：:Helicobacter pylori secretes a unique virulence factor, Tipalpha, that enters gastric cells and both stimulates the production of the TNF-alpha and activates the NF-kappaB pathway. The structures of a truncated version of Tipalpha (TipalphaN34) in two crystal forms are presented here. Tipalpha adopts a novel beta(1)al...

journal_title：FEBS letters

authors： Tosi T,Cioci G,Jouravleva K,Dian C,Terradot L

更新日期：2009-05-19 00:00:00

abstract：:The predicted Exigobacterium sibiricum bacterirhodopsin gene was amplified from an ancient Siberian permafrost sample. The protein bacteriorhodopsin from Exiguobacterium sibiricum (ESR) encoded by this gene was expressed in Escherichia coli membrane. ESR bound all-trans-retinal and displayed an absorbance maximum at 5...

journal_title：FEBS letters

authors： Petrovskaya LE,Lukashev EP,Chupin VV,Sychev SV,Lyukmanova EN,Kryukova EA,Ziganshin RH,Spirina EV,Rivkina EM,Khatypov RA,Erokhina LG,Gilichinsky DA,Shuvalov VA,Kirpichnikov MP

更新日期：2010-10-08 00:00:00

abstract：:The calcitonin receptor (CTR) gene in human osteoclasts formed in a human bone marrow cell culture system was examined by reverse transcription-polymerase chain reaction (RT-PCR). The RT-PCR results indicated that the 5'-untranslated region (5'UTR) was different between CTR mRNAs in human osteoclasts and in a mammary ...

journal_title：FEBS letters

authors： Nishikawa T,Ishikawa H,Yamamoto S,Koshihara Y

更新日期：1999-09-24 00:00:00

abstract：:The biochemical properties of the p51 subunit of HIV-1 reverse transcriptase (RT) were studied in order to understand its role in the heterodimeric form p66/p51 found in virions. A recombinant form of RT, p51/p51, expressed in yeast, was purified and characterized. The enzyme was affinity labeled using a 5' modified o...

journal_title：FEBS letters

authors： el Dirani-Diab R,Andreola ML,Nevinsky G,Tharaud D,Barr PJ,Litvak S,Tarrago-Litvak L

更新日期：1992-04-13 00:00:00

abstract：:Yeast Golgi ecto-ATPase Ynd1p is an unusual type III membrane protein with the longest translocated N-terminus reported. Sequential deletion analysis reveals that translocation of this 500-residue-long hydrophilic domain across the membranes requires the C-terminal transmembrane domain of Ynd1p and its flanking region...

journal_title：FEBS letters

authors： Zhong X,Malhotra R,Guidotti G

更新日期：2005-10-24 00:00:00

abstract：:It has previously been shown that the levels of poly(ADP-ribose)polymerase and polymers of ADP-ribose that co-purify with the nuclear matrix in regenerating liver fluctuate with the levels of in vivo DNA replication [(1988) FEBS Lett. 236, 362-366]. We have now electrophoretically identified lamins A and C, and poly(A...

journal_title：FEBS letters

authors： Pedraza-Reyes M,Alvarez-Gonzalez R

更新日期：1990-12-17 00:00:00

abstract：:Nitric oxide (.NO) synthase (NOS) activity in subcellular fractions from cultured endothelial cells (EC) and lipopolysaccharide-activated J774.2 monocyte/macrophages was investigated by monitoring the .NO-mediated increase in intracellular cyclic GMP in LLC-PK1 pig kidney epithelial cells. The constitutive NOS in EC (...

journal_title：FEBS letters

authors： Hecker M,Walsh DT,Vane JR

更新日期：1991-12-09 00:00:00

abstract：:The action of high-intensity ultraviolet pulse laser radiation on a 161 bp fragment of pBR 322 DNA (EcoRI-MspI fragment) was studied. At doses up to 5 X 10(18) photons/cm2 the N-glycosidic bond splitting is negligible. The action of hot piperidine on irradiated DNA leads to chain splitting at the residues, modified vi...

journal_title：FEBS letters

authors： Budowsky EI,Kovalsky OI,Yakovlev DYu,Simukova NA,Rubin LB

更新日期：1985-08-19 00:00:00

abstract：:Coagulation factor Va is an essential cofactor which combines with the serine protease factor Xa on a phospholipid surface to form the prothrombinase complex. In the present study, the structure of factor Va interacting with lipid surfaces containing phosphatidylserine was studied by electron microscopy. Two-dimension...

journal_title：FEBS letters

authors： Stoylova S,Mann KG,Brisson A

更新日期：1994-09-12 00:00:00

abstract：:A human teratoma cell line (Tera 2) was grown in serum-free medium, and the population multiplication was stimulated by the addition of 1-10 ng basic fibroblast growth factor (bFGF)/ml. The bFGF-effect was abrogated by the addition of protamine sulphate. When high concentrations of bFGF were added, a preferential effe...

journal_title：FEBS letters

authors： Schofield PN,Granerus M,Lee A,Ektröm TJ,Engström W

更新日期：1992-02-24 00:00:00

abstract：:Niemann-pick type C1 (NPC1) is an endo/lysosomal membrane protein involved in intracellular cholesterol trafficking, and its luminal domain C is an essential endosomal receptor for Ebola and Marburg viruses. We have determined the crystal structure of glycosylated NPC1 luminal domain C and find all seven possible site...

journal_title：FEBS letters

authors： Zhao Y,Ren J,Harlos K,Stuart DI

更新日期：2016-03-01 00:00:00

abstract：:The membrane-bound 3-chloro-4-hydroxyphenylacetate (Cl-OHPA) reductive dehalogenase from the chlorophenol-reducing anaerobe Desulfitobacterium hafniense was purified 11.3-fold to apparent homogeneity in the presence of the detergent CHAPS. The purified dehalogenase catalyzed the reductive dechlorination of Cl-OHPA to ...

journal_title：FEBS letters

authors： Christiansen N,Ahring BK,Wohlfarth G,Diekert G

更新日期：1998-10-02 00:00:00

abstract：:Respiratory complexes in both prokaryotes and eukaryotes contain multiple co-factors, which are coordinated in defined positions so that they can function as electron wires. Intriguingly, co-factors are usually buried deep within hetero-oligomeric protein complexes and it is not clear when or how they are incorporated...

journal_title：FEBS letters

authors： Palombo I,Daley DO

更新日期：2012-11-30 00:00:00

abstract：:Here we present a method to purify large amounts of highly pure and stably arrested ribosome-nascent chain complexes (RNCs) from Escherichia coli cells. It relies on the combined use of translation-arrest sequences to generate nascent polypeptides of specified length and subsequent tag purification of the RNCs. Moreov...

journal_title：FEBS letters

authors： Rutkowska A,Beerbaum M,Rajagopalan N,Fiaux J,Schmieder P,Kramer G,Oschkinat H,Bukau B

更新日期：2009-07-21 00:00:00

abstract：:The molecular mass of the rabbit prolactin receptor (rbPRLR) deduced from cDNA cloning is 66 kDa. However, the molecular mass of the full-length receptor expressed in the insect Sf9 cells was found to be 94 kDa. In order to explain this discrepancy, we analyzed the possible post-translational modifications of the PRLR...

journal_title：FEBS letters

authors： Cahoreau C,Garnier L,Djiane J,Devauchelle G,Cerutti M

更新日期：1994-08-22 00:00:00

abstract：:The involvement of H2O2 generated by photosystem II (PSII) in the process of photoinhibition of thylakoids with a functional oxygen-evolving complex (OEC) was investigated. The rate of photoinhibition was decreased to the rate of loss of activity in the dark when bovine Fe-catalase was present during the photoinhibito...

journal_title：FEBS letters

authors： Bradley RL,Long KM,Frasch WD

更新日期：1991-07-29 00:00:00

abstract：:Alternative splicing of tau exon 10 generates tau isoforms with three or four microtubule-binding repeats, named 3R- or 4R-tau. Normal adult human brain expresses equal levels of them. Imbalance of 3R-tau and 4R-tau associates with several tauopathies. Splicing factor 9G8 suppresses tau exon 10 inclusion and its funct...

journal_title：FEBS letters

authors： Gu J,Shi J,Wu S,Jin N,Qian W,Zhou J,Iqbal IG,Iqbal K,Gong CX,Liu F

更新日期：2012-07-30 00:00:00

abstract：:Thrombin has been shown to inhibit skeletal muscle differentiation. However, the mechanisms by which thrombin represses myogenesis remain unknown. Since the thrombin receptor couples to G(i), G(q/11) and G(12), we examined which subunits of heterotrimeric guanine nucleotide-binding regulatory proteins (Galpha(i), Galp...

journal_title：FEBS letters

authors： Nagao M,Kaziro Y,Itoh H

更新日期：2000-04-28 00:00:00

abstract：:Eucaryotic DNA is punctuated by many A+T-rich segments that we named A+T-rich linkers. Two types of these A+T-rich linkers can be distinguished: (i) isolated A+T-rich linkers, and (ii) A+T-rich linkers crowded in clusters. We have analysed the distribution of A+T-rich linker across the alpha- and beta-globin gene doma...

journal_title：FEBS letters

authors： Moreau J,Scherrer K

更新日期：1987-08-31 00:00:00

abstract：:On the basis of recent results, we propose a model for DNA polymerase translocation along DNA. Human immunodeficiency virus reverse transcriptase is taken as an example. According to the model, movement of the enzyme is the result of transition of the enzyme-bound DNA from the A- to B-form which is accompanied by leng...

journal_title：FEBS letters

authors： Wlassoff WA,Dymshits GM,Lavrik OI

更新日期：1996-07-15 00:00:00

abstract：:Proteolytic processing is an important post-translational modification affecting protein activity and stability. In the current study, we investigate the N-terminal cleavage of Trop2, a protein which is overexpressed in many cancers. We demonstrate that Trop2 is cleaved at Arg87 by a transmembrane serine protease, mat...

journal_title：FEBS letters

authors： Kamble PR,Rane S,Breed AA,Joseph S,Mahale SD,Pathak BR

更新日期：2020-10-01 00:00:00

abstract：:In HL-60 cells signal transduction via sphingomyelin hydrolysis (sphingomyelin cycle) is induced by binding of tumor necrosis factor alpha (TNFalpha) to cell surface TNFalpha receptor. We found that IgG immunoglobulins activate sphingomyelin hydrolysis in plasma membrane of HL-60 cells, with kinetics similar to that o...

journal_title：FEBS letters

authors： Glick D,Barenholz Y

更新日期：1996-10-07 00:00:00

abstract：:We used a xenograft model to investigate whether the aryl hydrocarbon receptor deletion construct CDelta553 suppresses tumor growth. HeLa cells that were infected with CDelta553 expressing adenovirus (Ad553) formed very small tumors whereas the control adenovirus-infected cells formed large tumors at day 15. CDelta553...

journal_title：FEBS letters

authors： Wang D,Faridi JS,Li Y,Chan WK

更新日期：2009-09-17 00:00:00

abstract：:The 39-kDa fragment of oat phytochrome phyA, obtained by tryptic digestion at the amino acids 65 and 425, was studied by resonance Raman spectroscopy. The parent state P(r) reveals far-reaching similarities with that of the native phytochrome implying that the structures of the tetrapyrrole chromophore and its immedia...

journal_title：FEBS letters

authors： Kneip C,Schlamann W,Braslavsky SE,Hildebrandt P,Schaffner K

更新日期：2000-10-06 00:00:00

abstract：:Iron dependent regulator, IdeR, regulates the expression of genes in response to intracellular iron levels in M. tuberculosis. Orthologs of IdeR are present in all the sequenced genomes of mycobacteria. We have used a computational approach to identify conserved IdeR regulated genes across the mycobacteria and the gen...

journal_title：FEBS letters

authors： Yellaboina S,Ranjan S,Vindal V,Ranjan A

更新日期：2006-05-15 00:00:00

abstract：:The hyperthermophilic archaeon Sulfolobus solfataricus metabolises glucose and galactose by a 'promiscuous' non-phosphorylative variant of the Entner-Doudoroff pathway, in which a series of enzymes have sufficient substrate promiscuity to permit the metabolism of both sugars. Recently, it has been proposed that the pa...

journal_title：FEBS letters

authors： Lamble HJ,Theodossis A,Milburn CC,Taylor GL,Bull SD,Hough DW,Danson MJ

更新日期：2005-12-19 00:00:00