Inhibition of Na+/H+ exchange reduces Ca2+ mobilization without affecting the initial cleavage of phosphatidylinositol 4,5-bisphosphate in thrombin-stimulated platelets.


:Stimulation of human platelets increases cytoplasmic pH (pHi) via activation of Na+/H+ exchange. We have determined the effect of inhibiting Na+/H+ exchange on (i) thrombin-induced Ca2+ mobilization and (ii) turnover of 32P-labelled phospholipids. Blocking Na+/H+ exchange by removal of extracellular Na+ or by ethylisopropylamiloride (EIPA) inhibited Ca2+ mobilization induced by 0.2 U/ml thrombin, whereas increasing pHi by NH4Cl enhanced the thrombin-induced increase in cytosolic free Ca2+. The effect of EIPA was bypassed after increasing pHi by moneasin. The thrombin-induced cleavage of phosphatidylinositol 4,5-bisphosphate (PIP2) was unaffected by treatments that blocked Na+/H+ exchange or increased pHi. It is concluded that activation of Na+/H+ exchange is a prerequisite for Ca2+ mobilization in human platelets but not for the stimulus-induced hydrolysis of PIP2.




FEBS letters


Siffert W,Siffert G,Scheid P,Riemens T,Gorter G,Akkerman JW




Has Abstract


1987-02-09 00:00:00














  • FixK₂, a key regulator in Bradyrhizobium japonicum, is a substrate for the protease ClpAP in vitro.

    abstract::FixK₂ is a CRP-like transcription factor that controls the endosymbiotic lifestyle of Bradyrhizobium japonicum. The reason for its noticeable protease sensitivity was explored here. The repertoire of Clp chaperone-proteases in B. japonicum was examined, and specifically ClpAP₁ and ClpXP₁ were purified and tested. FixK...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bonnet M,Stegmann M,Maglica Ž,Stiegeler E,Weber-Ban E,Hennecke H,Mesa S

    更新日期:2013-01-04 00:00:00

  • Stimulus-secretion coupling by high-affinity IgE receptor: new developments.

    abstract::Stimulation of mast cells through their high affinity IgE receptor (FcεRI) leads to the secretion of pre- and neoformed soluble mediators through vesicular carriers. This process is highly regulated in order to adapt the secretion of these potentially dangerous factors to the physiological needs. This regulation requi...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Benhamou M,Blank U

    更新日期:2010-12-15 00:00:00

  • Beyond the epithelium: cadherin function in fibrous connective tissues.

    abstract::In fibrous connective tissues, fibroblasts are organized into syncytia, cellular networks that enable matrix remodeling and that are interconnected by intercellular adherens junctions (AJs). The AJs of fibroblasts are mediated by N-cadherin, a broadly expressed classical cadherin that is critically involved in develop...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: El Sayegh TY,Kapus A,McCulloch CA

    更新日期:2007-01-23 00:00:00

  • Molecular cloning of cDNA encoding the 110 kDa and 21 kDa regulatory subunits of smooth muscle protein phosphatase 1M.

    abstract::The structures of the M110 and M21 regulatory subunits of protein phosphatase-1M, the major enzyme which dephosphorylates myosin in smooth muscle, have been deduced from cloned cDNAs. The N-terminus of the M110 subunit from rat aorta contains seven ankyrin repeats, while the C-terminus of the M21 subunit from chicken ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chen YH,Chen MX,Alessi DR,Campbell DG,Shanahan C,Cohen P,Cohen PT

    更新日期:1994-12-12 00:00:00

  • Pertussis toxin reverses the inhibition of insulin secretion caused by [Arg8]vasopressin in rat pancreatic islets.

    abstract::When rat pancreatic islets were incubated with 10(-8) M arginine vasopressin in the presence of 15 mM glucose there was a pronounced inhibition of insulin release in comparison with controls. This inhibitory effect appeared to be specific for vasopressin since it was antagonised by vasopressin antibody. Moreover, pert...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Khalaf LJ,Taylor KW

    更新日期:1988-04-11 00:00:00

  • An Escherichia coli cyoE gene homologue in thermophilic Bacillus PS3 encodes a thermotolerant heme O synthase.

    abstract::The cyoE gene of the Escherichia coli bo-type quinol oxidase operon (cyoABCDE) has been previously shown to encode heme O synthase. To demonstrate a catalytic role of a cyoE homologue (the caaE gene) in the gene cluster for caa3-type cytochrome c oxidase of thermophilic Bacillus PS3, we have carried out genetic comple...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Saiki K,Mogi T,Ishizuka M,Anraku Y

    更新日期:1994-09-12 00:00:00

  • Enhancement of phospholipase A2 activation by phosphatidic acid endogenously formed through phospholipase D action in rat peritoneal mast cell.

    abstract::Contribution of phosphatidic acid (PA) generated by activated phospholipase (PL) D to PLA2 activation was studied in rat peritoneal mast cells. Exogenous didecanoyl PA induced arachidonate liberation in the permeabilized cells which was inhibited by p-bromophenacyl bromide. Upon exposure of the cells to ethanol in a h...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sato T,Ishimoto T,Akiba S,Fujii T

    更新日期:1993-05-24 00:00:00

  • Staurosporine-related compounds, K252a and UCN-01, inhibit both cPKC and nPKC.

    abstract::The potent inhibitors of protein kinase C (PKC), H7, staurosporine, and staurosporine derivatives, were examined for their inhibitory effects on novel PKC (nPKC) isozymes delta and epsilon. H7 and staurosporine, usually used as selective inhibitors of PKC, showed similar inhibitory effects on cPKC (a mixture of cPKC a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mizuno K,Saido TC,Ohno S,Tamaoki T,Suzuki K

    更新日期:1993-09-13 00:00:00

  • Enhanced plasma ghrelin levels in rats with streptozotocin-induced diabetes.

    abstract::Ghrelin is a novel gastrointestinal peptide that stimulates growth hormone secretion, food intake, and body weight gain. Increased ghrelin secretion has been reported in such negative energy states as starvation and low body weight. We investigated the dynamics of ghrelin in rats with streptozotocin-induced diabetes, ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Masaoka T,Suzuki H,Hosoda H,Ota T,Minegishi Y,Nagata H,Kangawa K,Ishii H

    更新日期:2003-04-24 00:00:00

  • Identification of potential amino acid residues supporting anticodon recognition in yeast methionyl-tRNA synthetase.

    abstract::Sequence comparisons among methionyl-tRNA synthetases from different organisms reveal only one block of homology beyond the last beta strand of the mononucleotide fold. We have introduced a series of semi-conservative amino acid replacements in the conserved motif of yeast methionyl-tRNA synthetase. The results indica...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Despons L,Walter P,Senger B,Ebel JP,Fasiolo F

    更新日期:1991-09-09 00:00:00

  • Complex N-glycan promotes CD133 mono-ubiquitination and secretion.

    abstract::CD133 is a widely used cell surface marker of cancer stem cells that plays an important role in tumor initiation and metastasis. Increasing evidence shows that CD133 is secreted to the extracellular space. However, the underlying mechanisms of CD133 secretion remain largely unknown. In this study, we report that secre...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Li Y,Shi D,Yang F,Chen X,Xing Y,Liang Z,Zhuang J,Liu W,Gong Y,Jiang J,Wei Y

    更新日期:2019-04-01 00:00:00

  • Cell-cycle regulators are involved in transient cerebral ischemia induced neuronal apoptosis in female rats.

    abstract::Recent evidence indicates that cell-cycle regulating proteins are involved in apoptotic process in post-mitotic neurons. In this study, we examined cell-cycle regulators for G1/S cell-cycle progression after a transient focal cerebral ischemia induced by middle cerebral artery (MCA) occlusion. In the cerebral frontopa...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wen Y,Yang S,Liu R,Simpkins JW

    更新日期:2005-08-29 00:00:00

  • Leader sequence of a plant ribosomal protein gene with complementarity to the 18S rRNA triggers in vitro cap-independent translation.

    abstract::Cap-independent translation (CIT) occurs at the leader sequences of uncapped plant viral RNAs, but also at a number of normally capped cellular mRNAs and has been correlated with sequence complementarity to 18S rRNA. The ribosomal protein S18 (RPS18) is a component of the small ribosomal subunit and is encoded by thre...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vanderhaeghen R,De Clercq R,Karimi M,Van Montagu M,Hilson P,Van Lijsebettens M

    更新日期:2006-05-15 00:00:00

  • Human Tudor staphylococcal nuclease (Tudor-SN) protein modulates the kinetics of AGTR1-3'UTR granule formation.

    abstract::Human Tudor staphylococcal nuclease (Tudor-SN) interacts with the G3BP protein and is recruited into stress granules (SGs), the main type of discrete RNA-containing cytoplasmic foci structure that is formed under stress conditions. Here, we further demonstrate that Tudor-SN binds and co-localizes with AGTR1-3'UTR (3'-...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gao X,Shi X,Fu X,Ge L,Zhang Y,Su C,Yang X,Silvennoinen O,Yao Z,He J,Wei M,Yang J

    更新日期:2014-06-13 00:00:00

  • Ycf1p attenuates basal level oxidative stress response in Saccharomyces cerevisiae.

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    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Paumi CM,Pickin KA,Jarrar R,Herren CK,Cowley ST

    更新日期:2012-03-23 00:00:00

  • A bifunctional Galphai/Galphas modulatory peptide that attenuates adenylyl cyclase activity.

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    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Johnston CA,Ramer JK,Blaesius R,Fredericks Z,Watts VJ,Siderovski DP

    更新日期:2005-10-24 00:00:00

  • Differences in the regulation of microtubule stability by the pro-rich region variants of microtubule-associated protein 4.

    abstract::We have recently reported a neural variant of microtubule-associated protein 4 with a short pro-rich region (MAP4-SP). Here, we show that the neural MAP4 has reduced microtubule-stabilizing activity, compared to the ubiquitous MAP4 with a long pro-rich region (MAP4-LP), both in vitro and in vivo. Fluorescence recovery...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hasan MR,Jin M,Matsushima K,Miyamoto S,Kotani S,Nakagawa H

    更新日期:2006-06-12 00:00:00

  • Substituting selenocysteine for active site cysteine 149 of phosphorylating glyceraldehyde 3-phosphate dehydrogenase reveals a peroxidase activity.

    abstract::Replacing the essential Cys-149 by a selenocysteine into the active site of phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPDH) from Bacillus stearothermophilus leads to a selenoGAPDH that mimics a selenoperoxidase activity. Saturation kinetics were observed with cumenyl and tert-butyl hydroperoxides, wit...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Boschi-Muller S,Muller S,Van Dorsselaer A,Böck A,Branlant G

    更新日期:1998-11-20 00:00:00

  • Primary structure of matrilin-3, a new member of a family of extracellular matrix proteins related to cartilage matrix protein (matrilin-1) and von Willebrand factor.

    abstract::A mouse cDNA encoding for matrilin-3, the third member of the novel matrilin family of extracellular matrix proteins, was cloned. The protein precursor of 481 amino acids consists of a putative signal peptide, a short positively charged sequence, a single vWFA-like domain followed by four epidermal growth factor-like ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wagener R,Kobbe B,Paulsson M

    更新日期:1997-08-11 00:00:00

  • Differential stability of tetraspanin/tetraspanin interactions: role of palmitoylation.

    abstract::The tetraspanins associate with various surface molecules and with each other to build a network of molecular interactions, the tetraspanin web. The interaction of tetraspanins with each other seems to be central for the assembly of the tetraspanin web. All tetraspanins studied, CD9, CD37, CD53, CD63, CD81, CD82 and C...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Charrin S,Manié S,Oualid M,Billard M,Boucheix C,Rubinstein E

    更新日期:2002-04-10 00:00:00

  • Peroxiredoxin-I is an autoimmunogenic tumor antigen in non-small cell lung cancer.

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    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chang JW,Lee SH,Jeong JY,Chae HZ,Kim YC,Park ZY,Yoo YJ

    更新日期:2005-05-23 00:00:00

  • Backbone folding of the polypeptide cardiac stimulant anthopleurin-A determined by nuclear magnetic resonance, distance geometry and molecular dynamics.

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    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Torda AE,Mabbutt BC,van Gunsteren WF,Norton RS

    更新日期:1988-11-07 00:00:00

  • Metallothionein-III increases ADAM10 activity in association with furin, PC7, and PKCα during non-amyloidogenic processing.

    abstract::A-disintegrin and metalloproteinase 10 (ADAM10) is involved in the generation of amyloid-β (Aβ) during amyloid precursor protein (APP) processing, and has a protective effect against Aβ neurotoxicity. We explored how metallothionein-III (MT-III) is regulated in the non-amyloidogenic pathway to generate soluble APPα (s...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Park BH,Kim HG,Jin SW,Song SG,Jeong HG

    更新日期:2014-06-27 00:00:00

  • Silencing of the MT1-MMP/ G6PT axis suppresses calcium mobilization by sphingosine-1-phosphate in glioblastoma cells.

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    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fortier S,Labelle D,Sina A,Moreau R,Annabi B

    更新日期:2008-03-05 00:00:00

  • Induction of nuclear factor kappaB and activation protein 1 in postischemic myocardium.

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    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chandrasekar B,Freeman GL

    更新日期:1997-01-13 00:00:00

  • Alkaloid homoharringtonine inhibits polypeptide chain elongation on human ribosomes on the step of peptide bond formation.

    abstract::The aim of the present study was to investigate homoharringtonine alkaloid effect on: (i) the nonenzymatic and eEF-1-dependent Phe-tRNAPhe binding to poly(U)-programmed human placenta 80 S ribosomes; (ii) diphenylalanine synthesis accompanying nonenzymatic Phe-tRNAPhe binding; and (iii) acetylphenylalanyl-puromycin fo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tujebajeva RM,Graifer DM,Karpova GG,Ajtkhozhina NA

    更新日期:1989-11-06 00:00:00

  • Farnesylcysteine analogues inhibit chemotactic peptide receptor-mediated G-protein activation in human HL-60 granulocyte membranes.

    abstract::Analogues of S-prenylated cysteine like N-acetyl-S-trans,trans-farnesyl-L-cysteine (AFC) have previously been shown to inhibit the carboxyl methylation of proteins carrying a C-terminal S-prenylated cysteine residue and to block the endotoxin-activated serum-elicited chemotactic response of mouse macrophages. Here, we...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Scheer A,Gierschik P

    更新日期:1993-03-15 00:00:00

  • Interactions of the HIV-1 reverse transcriptase 'AZT-resistant' mutant with substrates and AZT-TP.

    abstract::To investigate the biochemical basis of the HIV-1 resistance to AZT we obtained the RT mutant containing four amino acid substitutions by an oligonucleotide-directed mutagenesis technique. Enzymatic properties of the wild type and mutant RTs were compared. 'AZT-resistant' mutations in RT were shown to be associated wi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pokholok DK,Gudima SO,Yesipov DS,Dobrynin VN,Rechinsky VO,Kochetkov SN

    更新日期:1993-07-05 00:00:00

  • Postnatal developmental profiles of filamentous actin and of 200 kDa neurofilament polypeptide in the visual cortex of light- and dark-reared rats and their relationship to critical period plasticity.

    abstract::The concept of critical period plasticity in rat visual cortex has been studied in terms of changes in the level of filamentous actin and of the 200 kDa neurofilament polypeptide. Our results suggest that the postnatal developmental profile of filamentous actin is affected by visual experience, as a consequence of eye...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nona SN,Trowell SC,Cronly-Dillon JR

    更新日期:1985-07-01 00:00:00

  • Eukaryotic selenocysteine tRNA has the 9/4 secondary structure.

    abstract::There are two secondary structure models for the eukaryotic selenocysteine (Sec) tRNA(Sec). One model, the 9/4 structure, was experimentally tested and possesses acceptor and T-stems with 9 and 4 bp, respectively [Sturchler et al., 1993; Hubert et al., 1998]. The other one, the 7/5 secondary structure with a bulge in ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mizutani T,Goto C

    更新日期:2000-01-28 00:00:00