Inhibition of Na+/H+ exchange reduces Ca2+ mobilization without affecting the initial cleavage of phosphatidylinositol 4,5-bisphosphate in thrombin-stimulated platelets.


:Stimulation of human platelets increases cytoplasmic pH (pHi) via activation of Na+/H+ exchange. We have determined the effect of inhibiting Na+/H+ exchange on (i) thrombin-induced Ca2+ mobilization and (ii) turnover of 32P-labelled phospholipids. Blocking Na+/H+ exchange by removal of extracellular Na+ or by ethylisopropylamiloride (EIPA) inhibited Ca2+ mobilization induced by 0.2 U/ml thrombin, whereas increasing pHi by NH4Cl enhanced the thrombin-induced increase in cytosolic free Ca2+. The effect of EIPA was bypassed after increasing pHi by moneasin. The thrombin-induced cleavage of phosphatidylinositol 4,5-bisphosphate (PIP2) was unaffected by treatments that blocked Na+/H+ exchange or increased pHi. It is concluded that activation of Na+/H+ exchange is a prerequisite for Ca2+ mobilization in human platelets but not for the stimulus-induced hydrolysis of PIP2.




FEBS letters


Siffert W,Siffert G,Scheid P,Riemens T,Gorter G,Akkerman JW




Has Abstract


1987-02-09 00:00:00














  • Microanalysis of the amino acid sequence of the eclosion hormone from the tobacco hornworm Manduca sexta.

    abstract::The amino acid sequence of the eclosion hormone from the tobacco hornworm Manduca sexta has been determined, using less than 500 pmol of protein and microanalytical techniques. The protein contains 62 amino acid residues and has a molecular mass of 6813 Da. The amino-terminal sequence is similar to that of a 13-residu...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Marti T,Takio K,Walsh KA,Terzi G,Truman JW

    更新日期:1987-07-27 00:00:00

  • Adrenergic regulation of ureogenesis in hepatocytes from adrenalectomized rats. Possible involvement of two pathways of signal transduction in alpha 1-adrenergic action.

    abstract::In hepatocytes from control rats, the ureogenic action of epinephrine is mainly mediated through alpha 1-adrenoceptors and the effect is independent of the presence of extracellular calcium. In hepatocytes from adrenalectomized rats, both alpha 1- and beta-adrenoceptors are involved in the action of epinephrine. Furth...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hernández-Sotomayor SM,García-Sáinz JA

    更新日期:1984-01-30 00:00:00

  • The prostate-specific antigen gene and the human glandular kallikrein-1 gene are tandemly located on chromosome 19.

    abstract::Using a prostate-specific antigen cDNA as a hybridization probe, clones containing the kallikrein genes encoding prostate-specific antigen, human glandular kallikrein-1 and pancreas/kidney kallikrein were isolated from a human genomic library. Clones containing the prostate-specific antigen gene and the human glandula...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Riegman PH,Vlietstra RJ,Klaassen P,van der Korput JA,Geurts van Kessel A,Romijn JC,Trapman J

    更新日期:1989-04-10 00:00:00

  • Identification of active site serine and histidine residues in Escherichia coli outer membrane protease OmpT.

    abstract::Escherichia coli outer membrane protease OmpT has been characterised as a serine protease based on its inhibitor profile, but serine protease consensus sequences are absent. By site-directed mutagenesis we substituted all conserved serines and histidines. Substitution of His(101) and His(212) by Ala, Asn or Gln result...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kramer RA,Dekker N,Egmond MR

    更新日期:2000-02-25 00:00:00

  • Analysis of gene expression in rose petals using expressed sequence tags.

    abstract::Single-pass sequences were obtained from the 5'-ends of a total of 1794 rose petal cDNA clones. Cluster analysis identified 242 groups of sequences and 635 singletons indicating that the database represents a total of 877 genes. Putative functions could be assigned to 1151 of the transcripts. Expression analysis indic...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Channelière S,Rivière S,Scalliet G,Szecsi J,Jullien F,Dolle C,Vergne P,Dumas C,Bendahmane M,Hugueney P,Cock JM

    更新日期:2002-03-27 00:00:00

  • Phosphorylation sites for ribosomal S6 protein kinases in mouse 3T3 fibroblasts stimulated with platelet-derived growth factor.

    abstract::Platelet release products and purified platelet-derived growth factor stimulated the phosphorylation of ribosomal protein S6 in cultured mouse Balb/c 3T3 fibroblasts. The post-nuclear fraction of the stimulated cells was enriched in S6 kinase activity specific for sites resembling those phosphorylated within intact ce...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wettenhall RE,Chesterman CN,Walker T,Morgan FJ

    更新日期:1983-10-03 00:00:00

  • Structure of the 16 S ribosomal RNA of the thermophilic cyanobacterium Chlorogloeopsis HTF ('Mastigocladus laminosus HTF') strain PCC7518, and phylogenetic analysis.

    abstract::The thermophilic cyanobacterial strain, PCC7518, originally identified as 'Mastigocladus laminosus HTF' does not show branchings or heterocysts. The absence of branchings supports the later assignment to the genus Chlorogloeopsis. The absence of heterocysts may be the result of a mutation because heterocysts were obse...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wilmotte A,Van der Auwera G,De Wachter R

    更新日期:1993-02-08 00:00:00

  • Effects of Ca2+ and a phorbol ester on insulin secretion from islets of Langerhans permeabilised by high-voltage discharge.

    abstract::Isolated rat islets of Langerhans permeabilised by high-voltage discharge secreted insulin in response to elevations in Ca2+ over the range 100 nM to 10 microM Ca2+. The phorbol ester, 12-O-tetradecanoylphorbol 13-acetate (TPA), had no effects on insulin secretion in the absence of Ca2+. In the presence of Ca2+ concen...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Jones PM,Stutchfield J,Howell SL

    更新日期:1985-10-21 00:00:00

  • Comparison of the current RefSeq, Ensembl and EST databases for counting genes and gene discovery.

    abstract::Large amounts of refined sequence material in the form of predicted, curated and annotated genes and expressed sequences tags (ESTs) have recently been added to the NCBI databases. We matched the transcript-sequences of RefSeq, Ensembl and dbEST in an attempt to provide an updated overview of how many unique human gen...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Larsson TP,Murray CG,Hill T,Fredriksson R,Schiöth HB

    更新日期:2005-01-31 00:00:00

  • Regulation of protein synthesis in lymphoblastoid cells during inhibition of cell proliferation by human interferons.

    abstract::Treatment of human lymphoblastoid (Daudi) cells with interferons inhibits cell proliferation in culture within 24 h. The failure of cell growth has been shown to be associated with impaired processing and decreased stability of newly replicated DNA. Because there is a close relationship between DNA replication and pro...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Clemens MJ,McNurlan MA,Moore G,Tilleray VJ

    更新日期:1984-06-04 00:00:00

  • Aging decreases the abundance of retinoic acid (RAR) and triiodothyronine (TR) nuclear receptor mRNA in rat brain: effect of the administration of retinoids.

    abstract::Aging is accompanied by troubles resulting from changes in hormonal and nutritional status. Therefore, the abundance of mRNA coding for triiodothyronine (TR) and retinoic acid (RA) nuclear receptors was studied in the brain of young, adult and aged (2.5, 6 and 24 months, respectively) rats. In the brain of aged rats, ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Enderlin V,Alfos S,Pallet V,Garcin H,Azaïs-Braesco V,Jaffard R,Higueret P

    更新日期:1997-08-04 00:00:00

  • Functional argument for the existence of an avian nitric oxide synthase in muscle mitochondria: effect of cold acclimation.

    abstract::We report the first evidence of a mitochondrial NO synthase (mtNOS) in bird skeletal muscle. In vitro, mtNOS activity stimulated by L-arginine reduced intermyofibrillar mitochondrial oxygen uptake and ATP synthesis rates, stimulated endogenous H(2)O(2) generation, but had no effect on oxidative phosphorylation efficie...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rey B,Roussel D,Teulier L,Eyenga P,Degletagne C,Belouze M,Duchamp C

    更新日期:2011-01-03 00:00:00

  • Importance of local structures of second and third repeat fragments of microtubule-binding domain for tau filament formation.

    abstract::To investigate the importance of the seventh residue of the second and third repeat fragments (R2 and R3 peptides) of the microtubule-binding domain (MBD) for tau filamentous assembly, the residues Lys and Pro were substituted (R2-K7P and R3-P7K). The filament formations of the R2 and R3 peptides were almost lost due ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tokimasa M,Minoura K,Hiraoka S,Tomoo K,Sumida M,Taniguchi T,Ishida T

    更新日期:2005-07-04 00:00:00

  • Mutation of an N-terminal acidic-rich region of p115-RhoGEF dissociates alpha13 binding and alpha13-promoted plasma membrane recruitment.

    abstract::The Ras homology (Rho) guanine nucleotide exchange factor p115-RhoGEF couples the alpha(13) heterotrimeric guanine nucleotide binding protein (G protein) subunit to Rho GTPase. Alpha(13) binds to a regulator of G protein signaling (RGS) domain in p115-RhoGEF, but the mechanism of alpha(13) activation of p115-RhoGEF is...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bhattacharyya R,Wedegaertner PB

    更新日期:2003-04-10 00:00:00

  • The cosubstrate NADP(H) protects lysine 601 in the porcine NADPH-cytochrome P-450 reductase against pyridoxylation.

    abstract::Lys601 in NADPH-cytochrome P-450 reductase is modified by reductive alkylation with pyridoxal 5'-phosphate (pyridoxylation). Lys601 is protected against modification by the cosubstrate NADP(H). ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Müller K,Linder D,Lumper L

    更新日期:1990-01-29 00:00:00

  • Alterations in expression of E2F-1 and E2F-responsive genes by RB, p53 and p21(Sdi1/WAF1/Cip1) expression.

    abstract::RB, p53 and p21(Sdi1/WAF1/Cip1) interact in the induction of G1 arrest. We established osteosarcoma cell lines in which a tetracycline-regulatable promoter controls the induction of RB, p53 and p21. By using these cell lines, we investigated whether RB, p53 or p21 regulates, in the same manner or differently, expressi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ookawa K,Tsuchida S,Kohno T,Yokota J

    更新日期:2001-06-29 00:00:00

  • A cytochrome c-GFP fusion is not released from mitochondria into the cytoplasm upon expression of Bax in yeast cells.

    abstract::To study Bax-induced release of cytochrome c in vivo, we have expressed a cytochrome c-GFP (green fluorescent protein) fusion in Saccharomyces cerevisiae cells null for the expression of the endogenous cytochrome. We show here that cytochrome c-GFP is efficiently localised to mitochondria and able to function as an el...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Roucou X,Prescott M,Devenish RJ,Nagley P

    更新日期:2000-04-14 00:00:00

  • COP9 signalosome function in the DDR.

    abstract::The COP9 signalosome (CSN) is a platform for protein communication in eukaryotic cells. It has an intrinsic metalloprotease that removes the ubiquitin (Ub)-like protein Nedd8 from cullins. CSN-mediated deneddylation regulates culling-RING Ub ligases (CRLs) and controls ubiquitination of proteins involved in DNA damage...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Hannss R,Dubiel W

    更新日期:2011-09-16 00:00:00

  • The X gene of hepatitis B virus shows a high level stimulation of the Rous sarcoma virus long terminal repeat in the methylotropic yeast, Pichia pastoris.

    abstract::In order to study the transactivational property of the X gene in the methylotropic yeast Pichia pastoris, a Rous sarcoma virus-chloramphenicol acetyltransferase (RSV-CAT) cassette was co-transformed and integrated into the host yeast strain as a reporter which showed an overwhelming CAT activity. Immunoprecipitation ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lal SK,Kumar V

    更新日期:1999-07-30 00:00:00

  • Folding kinetics of the lipoic acid-bearing domain of human mitochondrial branched chain alpha-ketoacid dehydrogenase complex.

    abstract::A reversible two-step (native state<-->denatured state) folding mechanism based on equilibrium and stopped flow experiments is proposed for urea denaturation of the lipoyl-bearing domain (hbLBD) of human mitochondrial branched chain alpha-ketoacid dehydrogenase (BCKD) complex. The results from this circular dichroism ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Naik MT,Chang YC,Huang TH

    更新日期:2002-10-23 00:00:00

  • The Zβ domain of human DAI binds to Z-DNA via a novel B-Z transition pathway.

    abstract::The human DNA-dependent activator of IFN-regulatory factor (DAI) protein, which activates the innate immune response in response to DNA, contains two tandem Z-DNA binding domains (Zα and Zβ) at the NH(2) terminus. The hZβ(DAI) structure is similar to other Z-DNA binding proteins, although it demonstrates an unusual Z-...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kim HE,Ahn HC,Lee YM,Lee EH,Seo YJ,Kim YG,Kim KK,Choi BS,Lee JH

    更新日期:2011-03-09 00:00:00

  • Transbilayer reorientation of platelet-activating factor in the erythrocyte membrane.

    abstract::Platelet-activating factor (PAF) is a phospholipid, intermediate in its structure between diacyl- and lysophospholipids. The reorientation of this highly bioactive compound to the inner membrane layer of human erythrocytes after its primary incorporation into the outer membrane layer has been studied. Reorientation wa...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schneider E,Haest CW,Deuticke B

    更新日期:1986-03-31 00:00:00

  • Expression and biochemical analysis of the entire HIV-2 gp41 ectodomain: determinants of stability map to N- and C-terminal sequences outside the 6-helix bundle core.

    abstract::The folding of HIV gp41 into a 6-helix bundle drives virus-cell membrane fusion. To examine the structural relationship between the 6-helix bundle core domain and other regions of gp41, we expressed in Escherichia coli, the entire ectodomain of HIV-2(ST) gp41 as a soluble, trimeric maltose-binding protein (MBP)/gp41 c...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lay CS,Wilson KA,Kobe B,Kemp BE,Drummer HE,Poumbourios P

    更新日期:2004-06-04 00:00:00

  • The influence of the chain length of aldehydes on the fluorescence of chromolipids.

    abstract::Incubation of phosphatidylethanolamine containing liposomes with malondialdehyde and other aldehydes with different chain lengths results in the fluorescence of chromolipids. Relative to malondialdehyde, the fluorescence was greatly enhanced with increasing chain length upon incubation of 2-alkenals with phospholipids...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Montfoort A,Bezstarosti K,Groh MM,Koster JF

    更新日期:1987-12-21 00:00:00

  • Tubulin and microtubule are potential targets for brain hexokinase binding.

    abstract::The metabolite-modulated association of a fraction of hexokinase to mitochondria in brain is well documented, however, the involvement of other non-mitochondrial components in the binding of the hexokinase is controversial. Now we present evidence that the hexokinase binds both tubulin and microtubules in brain in vit...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wágner G,Kovács J,Löw P,Orosz F,Ovádi J

    更新日期:2001-11-30 00:00:00

  • Inhibition of Na,K-ATPase activity by cGMP is isoform-specific in brain endothelial cells.

    abstract::cGMP has been shown to either activate or inhibit Na,K-ATPase activity. Using mouse brain endothelial cells which express both ouabain-resistant alpha1 and ouabain-sensitive alpha2 and alpha3 isoforms, we show that cGMP reduces total Na,KATPase activity to about 58%. The inhibition is prevented by the protein kinase G...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pontiggia L,Winterhalter K,Gloor SM

    更新日期:1998-10-09 00:00:00

  • Characterization of glucose transport system in Drosophila Kc cells.

    abstract::We have characterized the glucose transport system in the Kc cells of Drosophila melanogaster. The transporter is stereospecific and can be inhibited by both cytochalasin B and phloretin, with ki values of 4 microM and 30 microM for cytochalasin B and phloretin, respectively. Moreover, phlordzin does not affect uptake...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wang M,Wang C

    更新日期:1993-02-15 00:00:00

  • CFTR mRNA and its truncated splice variant (TRN-CFTR) are differentially expressed during collecting duct ontogeny.

    abstract::The collecting duct epithelium originates from the embryonic ureter by branching morphogenesis. Ontogeny-dependent changes of CFTR mRNA expression were assessed by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) in primary monolayer cultures of rat ureteric buds (UB) and cortical collecting ducts...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Huber S,Braun G,Burger-Kentischer A,Reinhart B,Luckow B,Horster M

    更新日期:1998-02-27 00:00:00

  • Comparative pharmacology and cloning of two novel arachnid sodium channels: Exploring the adaptive insensitivity of scorpion to its toxins.

    abstract::Scorpion toxins have been found lacking effect on Na(+) current of its own sodium channel, whereas the molecular mechanism remains mystery. In this study, the binding affinity of pharmacologically distinct scorpion toxins was found much weaker to scorpion (Buthus martensii) nerve synaptosomes than to spider (Ornithoct...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zuo XP,He HQ,He M,Liu ZR,Xu Q,Ye JG,Ji YH

    更新日期:2006-08-07 00:00:00

  • Hypoxia-inducible factor-1alpha regulates matrix metalloproteinase-1 activity in human bone marrow-derived mesenchymal stem cells.

    abstract::We examined the mRNA levels of hypoxia-inducible factor-1alpha (HIF-1alpha) in bone marrow mesenchymal stem cells (bmMSCs) of eight osteoarthritis patients. BmMSC-1, expressing higher HIF-1alpha mRNA and protein than bmMSC-5, elicited higher matrix metalloproteinase-1 (MMP1) activity and stronger invasive capacity. In...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lin JL,Wang MJ,Lee D,Liang CC,Lin S

    更新日期:2008-07-23 00:00:00