Abstract:
:Stimulation of human platelets increases cytoplasmic pH (pHi) via activation of Na+/H+ exchange. We have determined the effect of inhibiting Na+/H+ exchange on (i) thrombin-induced Ca2+ mobilization and (ii) turnover of 32P-labelled phospholipids. Blocking Na+/H+ exchange by removal of extracellular Na+ or by ethylisopropylamiloride (EIPA) inhibited Ca2+ mobilization induced by 0.2 U/ml thrombin, whereas increasing pHi by NH4Cl enhanced the thrombin-induced increase in cytosolic free Ca2+. The effect of EIPA was bypassed after increasing pHi by moneasin. The thrombin-induced cleavage of phosphatidylinositol 4,5-bisphosphate (PIP2) was unaffected by treatments that blocked Na+/H+ exchange or increased pHi. It is concluded that activation of Na+/H+ exchange is a prerequisite for Ca2+ mobilization in human platelets but not for the stimulus-induced hydrolysis of PIP2.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Siffert W,Siffert G,Scheid P,Riemens T,Gorter G,Akkerman JWdoi
10.1016/0014-5793(87)81569-7subject
Has Abstractpub_date
1987-02-09 00:00:00pages
123-6issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(87)81569-7journal_volume
212pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::We have purified a 38 kDa protein from bovine brain which is cross-reactive with an affinity purified antibody against the 35 kDa phosphatidylinositol transfer protein from the same source. Controlled trypsinization of the 38 kDa protein yielded an immunoreactive protein of 35 kDa which displayed a 6-fold increase in ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80523-l
更新日期:1990-12-10 00:00:00
abstract::Vascular endothelial growth factor (VEGF) mRNA expression was analysed in rabbit vascular smooth muscle cells following exposure to hypoxia and platelet-derived growth factor-BB (PDGF-BB). Hypoxia potently upregulated VEGF mRNA steady-state levels in a time- and concentration-dependent manner reaching a maximum level ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01458-d
更新日期:1995-01-30 00:00:00
abstract::Nine of the cbi genes from the 17.5 kb cob operon of Salmonella typhimurium previously shown by genetic studies to be involved in the biosynthesis of cobinamide from precorrin-2, have been subcloned and expressed in Escherichia coli. Seven of the gene products were found in the soluble fraction of cell lysates and hav...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80213-z
更新日期:1992-04-13 00:00:00
abstract::Nitric oxide (.NO) synthase (NOS) activity in subcellular fractions from cultured endothelial cells (EC) and lipopolysaccharide-activated J774.2 monocyte/macrophages was investigated by monitoring the .NO-mediated increase in intracellular cyclic GMP in LLC-PK1 pig kidney epithelial cells. The constitutive NOS in EC (...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81434-a
更新日期:1991-12-09 00:00:00
abstract::The four chain agglutinins from Abrus precatorius, Viscum album and Ricinus communis promote depurination of the 28 S rRNA from rabbit reticulocyte ribosomes characteristic of the common ribosome-inactivating proteins (RIPs). These agglutinins inhibited mammalian protein synthesis at nanomolar concentrations but they ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80193-x
更新日期:1993-08-23 00:00:00
abstract::The gene for human tetranectin was isolated from a genomic library with a mixture of degenerate oligonucleotide probes. The gene is about 12 kbp and contains two intervening sequences. The gene encodes a protein of 202 amino acid residues, with a signal peptide of 21 amino acid residues, followed by the tetranectin se...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80729-z
更新日期:1992-08-31 00:00:00
abstract::The inositol (1,4,5)-trisphosphate receptor (InsP3R) is an intracellular calcium release channel that plays a crucial role in cell signaling. In Drosophila melanogaster, a single InsP3R gene (itpr) encodes a protein (DmInsP3R) that is approximately 60% conserved with mammalian InsP3Rs. The functional properties of wil...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.08.042
更新日期:2004-09-24 00:00:00
abstract::As a member of the dynamin superfamily human guanylate-binding protein 1 (hGBP1) binds and hydrolyses GTP thereby undergoing structural changes which lead to self-assembly of the protein. Here, we employ the reactivity of hGBP1 with a cysteine reactive compound in order to monitor structural changes imposed by GTP bin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.05.027
更新日期:2009-06-18 00:00:00
abstract::It has been suggested that the C-terminal domain of Bcl-2 family members may contain a signal anchor sequence that targets these proteins to the mitochondrial outer membrane. We have investigated the consequence of deleting this domain upon cytochrome c release in yeast strains that coexpress truncated forms of Bax (i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01661-5
更新日期:1999-01-25 00:00:00
abstract::Human uncoupling protein 3 (UCP3) has two RNA transcripts that arise from the differential processing of the same gene product. One encodes the full length protein (UCP3L) while the other encodes a truncated version (UCP3S) lacking the sixth membrane spanning domain. The roles of the two isoforms are not known, but a ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01688-9
更新日期:2000-01-14 00:00:00
abstract::Plasmids for the high-level overproduction of wild-type, and C- and N-terminal His-tagged MurG N-acetylglucosaminyl transferase from Escherichia coli were constructed. In complementation tests the three forms were active in vivo. After IPTG induction, growth, spheroplast formation and lysis, overproduced MurG proteins...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00412-3
更新日期:1999-04-23 00:00:00
abstract::Chronic myelogenous leukemia (CML) is associated with the presence of the Philadelphia chromosome, which is generated by the reciprocal translocation of chromosomes 9 and 22. In the case of L6 (b2a2) mRNA, it is difficult to cleave the abnormal mRNA specifically because the mRNA includes no sequences that can be cleav...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01367-8
更新日期:1999-11-12 00:00:00
abstract::Endothelial cells play a pivotal role in the development of atherosclerosis. An 'activated' phenotype of these cells is manifested by signal transduction-dependent expression of genes encoding cytokines, pro- and anticoagulant factors, and cell adhesion molecules. In the current study we examined the effect of ouabain...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01301-6
更新日期:1995-12-11 00:00:00
abstract::Cell stimulation raised the cytosolic free Ca2+ concentration, [Ca(2+]i and induces activation of Na+/H+ exchange which raises the cytosolic pH, pHi. Recent studies have addressed the question whether Na+/H+ exchange plays a role in Ca(2+) influx and, specifically, whether a rise in pHi alone suffices to open Ca(2+) c...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(89)81480-2
更新日期:1989-12-18 00:00:00
abstract::A structural tree for proteins and domains containing S-like beta-sheets has been constructed. An S-like beta-sheet is taken as a starting structure in modelling or as a root structure of the tree. Larger structures are obtained by a stepwise addition of beta-strands and/or alpha-helices to the root S-like beta-sheet ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01244-7
更新日期:1998-10-23 00:00:00
abstract::Extracellular ATP itself elicited the generation of superoxide (O2-) in guinea pig peritoneal macrophages associated with an increase in cytosolic calcium ([Ca2+]i). The ATP-induced O2- generation was completely inhibited by pretreatment with pertussis toxin (PT) accompanied by the suppression of [Ca2+]i mobilization....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80451-8
更新日期:1991-04-22 00:00:00
abstract::A stable complex of the chaperonins, cpn60 and cpn10 (Escherichia coli GroEL and GroES homologues), from the extremely thermophilic bacterium Thermus thermophilus has been isolated and crystallized. The crystals have dimensions up to 30 x 200 x 200 microns. Ultra-thin sections of the crystals estimated by electron mic...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81357-r
更新日期:1992-10-12 00:00:00
abstract::Phospholipase D (PLD) has been associated with necrosis. However, it is not clear whether PLD plays a causative role in this cellular process. We investigated the role of PLD in oxidative stress-induced necrosis of vascular smooth muscle cells (VSMCs). Pervanadate (hydrogen peroxide plus orthovanadate) but not hydroge...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03059-9
更新日期:2001-11-16 00:00:00
abstract::Protective antigen (PA) is the central receptor binding component of anthrax toxin, which translocates catalytic components of the toxin into the cytosol of mammalian cells. Ever since the crystal structure of PA was solved, there have been speculations regarding the possible role of calcium ions present in domain I o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01226-2
更新日期:2003-11-20 00:00:00
abstract::Glycoprotein synthesis is initiated in the endoplasmic reticulum (ER) lumen upon transfer of a glycan (Glc3Man9GlcNAc2) from a lipid derivative to Asn residues (N-glycosylation). N-Glycan-dependent quality control of glycoprotein folding in the ER prevents exit to Golgi of folding intermediates, irreparably misfolded ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2015.07.021
更新日期:2015-11-14 00:00:00
abstract::Synthetic high- and low-molecular-mass atrial peptides were phosphorylated in vitro by cyclic AMP-dependent protein kinase and [32P]ATP. From a series of atrial peptide analogs, it was deduced that the amino acid sequence, Arg101-Ser104 of atriopeptin was required for optimal phosphorylation. Phosphorylated AP(99-126)...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80478-7
更新日期:1987-11-30 00:00:00
abstract::The ATP synthase capacity of rat heart myocytes can be measured in sonicates of cultured cardiomyocytes. In these cells, transitions in ATP synthase capacity occur on changing to the anoxic or uncoupled state (drop in ATP synthase capacity of over 40%) or on electrically stimulating the cells to contract (rise of 70%)...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81725-9
更新日期:1989-10-09 00:00:00
abstract::The cDNA coding for a novel member of the cysteine-rich protein family was isolated from a rat brain cDNA library. It encodes a protein, denoted cysteine-rich protein 2 (CRP2), of 208 amino acid residues containing two tandem repeats of an unusual LIM/double zinc-finger-like motif. The ubiquitous tissue distribution a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80373-3
更新日期:1993-10-25 00:00:00
abstract::Recombinant porcine growth hormone (rPGH) was solubilised from inclusion bodies (IB's) using either 6 M guanidinium hydrochloride (GnHCl), 7.5 M urea or by a novel method using a cationic surfactant, cetyltrimethylammonium chloride (CTAC). Circular dichroism (CD) analysis of the secondary (2 degrees) structure of the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80661-y
更新日期:1992-07-06 00:00:00
abstract::In eukaryotic cells, peroxiredoxins are both antioxidants and regulators of H(2)O(2)-mediated signaling. We previously found that peroxiredoxin-I (Prx-I) was overexpressed in non-small cell lung cancer (NSCLC) tissue. Since overexpressed protein can induce a humoral immune response, we examined whether serum from NSCL...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.04.028
更新日期:2005-05-23 00:00:00
abstract::Evidence has previously suggested that cellobiose:quinone oxidoreductase (CBQ) in cellulolytic cultures of Phanerochaete chrysosporium might be produced from cellobiose oxidase (CBO) by proteolytic cleavage. This study demonstrates that the ratio of CBO activity to (CBO + CBQ) activity declines with decreasing culture...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80162-n
更新日期:1993-07-26 00:00:00
abstract::The present study discusses the efficacy of Aloe emodin-8-O-glycoside (AEG), a plant derived anthroquinone, on alleviating insulin resistance and augmenting glycogen synthesis in L6 myotubes and 3T3L1 adipocytes. Dose-dependent increase in glucose uptake activity (GUA) was observed in both cell lines. Immunoblot analy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.06.004
更新日期:2010-07-16 00:00:00
abstract::The role of the Ca(2+)-activated tyrosine kinase, Pyk2, in the pleiotropic coupling of nerve cell stimulation to the MAP kinase cascade still remains undefined. Using a panel of PC12 clones, one of which was defective in Pyk2, we demonstrate (1) that the MAP kinase response induced by a [Ca(2+)](i) rise (following app...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01468-4
更新日期:1999-11-19 00:00:00
abstract::Monocyte chemoattractant protein-1 (MCP-1) is a member of the beta chemokine family which acts through specific seven transmembrane receptors to recruit monocytes, basophils, and T lymphocytes to sites of inflammation. To identify regions of the human MCP-1 protein which are important for its biological activity, we h...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00637-1
更新日期:1998-07-03 00:00:00
abstract::For understanding the mechanism(s) relating inflammation to corticosteroid action, the effect of tumour necrosis factor-alpha (TNF-alpha) on 11beta-hydroxysteroid dehydrogenase type 2 (11beta-HSD2), the enzyme regulating access of 11beta-hydroxycorticosteroids to receptors, was studied in LLC-PK(1) cells. We observed ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03004-6
更新日期:2001-11-02 00:00:00