Abstract:
:Stimulation of human platelets increases cytoplasmic pH (pHi) via activation of Na+/H+ exchange. We have determined the effect of inhibiting Na+/H+ exchange on (i) thrombin-induced Ca2+ mobilization and (ii) turnover of 32P-labelled phospholipids. Blocking Na+/H+ exchange by removal of extracellular Na+ or by ethylisopropylamiloride (EIPA) inhibited Ca2+ mobilization induced by 0.2 U/ml thrombin, whereas increasing pHi by NH4Cl enhanced the thrombin-induced increase in cytosolic free Ca2+. The effect of EIPA was bypassed after increasing pHi by moneasin. The thrombin-induced cleavage of phosphatidylinositol 4,5-bisphosphate (PIP2) was unaffected by treatments that blocked Na+/H+ exchange or increased pHi. It is concluded that activation of Na+/H+ exchange is a prerequisite for Ca2+ mobilization in human platelets but not for the stimulus-induced hydrolysis of PIP2.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Siffert W,Siffert G,Scheid P,Riemens T,Gorter G,Akkerman JWdoi
10.1016/0014-5793(87)81569-7subject
Has Abstractpub_date
1987-02-09 00:00:00pages
123-6issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(87)81569-7journal_volume
212pub_type
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