Functional properties of a pore mutant in the Drosophila melanogaster inositol 1,4,5-trisphosphate receptor.


:The inositol (1,4,5)-trisphosphate receptor (InsP3R) is an intracellular calcium release channel that plays a crucial role in cell signaling. In Drosophila melanogaster, a single InsP3R gene (itpr) encodes a protein (DmInsP3R) that is approximately 60% conserved with mammalian InsP3Rs. The functional properties of wild-type (WT) and mutant DmInsP3Rs have recently been described [Srikanth et al., Biophys. J. 86 (2004) 3634-3646]. Here, we use the planar lipid bilayer reconstitution technique to describe single channel properties of a ka901 point mutant (G2630S) in the pore-forming region of DmInsP3R. We find that homomeric ka901 channels are not functional, but the heteromeric WT:ka901 mutant channels display increased conductance, longer channel open time and altered ion selectivity properties when compared to WT DmInsP3R. Obtained results are consistent with the gain of function phenotype observed in ka901/+ mutant flies.




FEBS letters


Srikanth S,Wang Z,Hasan G,Bezprozvanny I





Has Abstract


2004-09-24 00:00:00














  • A novel antimicrobial peptide from the loach, Misgurnus anguillicaudatus.

    abstract::A novel antimicrobial peptide, named misgurin, was isolated and characterized from the loach (mudfish), Misgurnus anguillicaudatus. The 21-amino-acid peptide with a molecular mass of 2502 Da was purified to homogeneity using a heparin-affinity column and C18 reverse-phase and gel-permeation high-performance liquid chr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Park CB,Lee JH,Park IY,Kim MS,Kim SC

    更新日期:1997-07-14 00:00:00

  • A novel subunit of axonemal dynein conserved among lower and higher eukaryotes.

    abstract::To elucidate the subunit composition of axonemal inner-arm dynein, we examined a 38 kDa protein (p38) co-purified with a Chlamydomonas inner arm subspecies, dynein d. We found it is a novel protein conserved among a variety of organisms with motile cilia and flagella. Immunoprecipitation using specific antibody verifi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yamamoto R,Yanagisawa HA,Yagi T,Kamiya R

    更新日期:2006-11-27 00:00:00

  • Multiple genes for G protein-coupled receptors and their expression in lingual epithelia.

    abstract::Using the polymerase chain reaction (PCR), we identified a gene family including more than 60 members which encoded similar G protein-coupled seven-transmembrane receptors. Sequence analyses of six representatives out of the 60 PCR clones showed that they had significant structural similarity to olfactory and optic re...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Abe K,Kusakabe Y,Tanemura K,Emori Y,Arai S

    更新日期:1993-02-01 00:00:00

  • Crystallization and crystallographic investigations of the small subunit of mouse ribonucleotide reductase.

    abstract::The R2 protein component of mouse ribonucleotide reductase has been obtained from overproducing Escherichia coli bacteria. It has been crystallized using NaCl as precipitant. The crystals are orthorhombic, space group C222(1), with cell dimensions a = 76.9 A, b = 108.9 A, c = 92.7 A and diffract to at least 2.5 A. The...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nielsen BB,Kauppi B,Thelander M,Thelander L,Larsen IK,Eklund H

    更新日期:1995-10-16 00:00:00

  • Complete amino acid sequence of human seminal plasma beta-inhibin. Prediction of post Gln-Arg cleavage as a maturation site.

    abstract::The complete sequence of a 94 amino acid human seminal plasma polypeptide exhibiting inhibin-like activity is presented. This molecule, called beta-inhibin, selectively and specifically suppresses the release of pituitary FSH in vivo as well as in vitro. It does not affect the secretion of LH. Such a novel acidic prot...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Seidah NG,Arbatti NJ,Rochemont J,Sheth AR,Chrétien M

    更新日期:1984-10-01 00:00:00

  • Microtubule-active drugs suppress the closure of the permeability transition pore in tumour mitochondria.

    abstract::We report the effects of anticancer drugs, inhibitors of microtubule organisation, on the mitochondrial permeability transition pore (PTP) in Ehrlich ascites tumour cells. Taxol (5-20 microM) and colchicine (100-500 microM) prevented closing of the cyclosporin A-sensitive PTP. No taxol or colchicine effects on oxidati...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Evtodienko YV,Teplova VV,Sidash SS,Ichas F,Mazat JP

    更新日期:1996-09-09 00:00:00

  • L protein, encoded by psbL, restores normal functioning of the primary quinone acceptor, QA, in isolated D1/D2/CP47/Cytb-559/I photosystem II reaction center core complex.

    abstract::Plastoquinone-9 (PQ-9)-depleted PSII reaction center core complex, consisting of CP47/D1/D2/Cytb-559/I, was isolated from spinach PSII particles. PQ-9, lipids and several proteins were extracted from the original PSII particles and separated by several steps of chromatography to be reconstituted into the isolated comp...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kitamura K,Ozawa S,Shiina T,Toyoshima Y

    更新日期:1994-10-31 00:00:00

  • A reporter for dsRNA response in Neurospora crassa.

    abstract::In the filamentous fungus Neurospora, the production of dsRNA can elicit a dsRNA-induced transcriptional response similar to the interferon response in vertebrates. However, how fungi sense the expression of dsRNA and activate gene expression is unknown. In this study, we established a dsRNA response reporter system i...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Li X,Shen Y,Sun J,Wang B,He Q

    更新日期:2011-03-23 00:00:00

  • Distinct phospholipase C-regulated signalling pathways in Swiss 3T3 fibroblasts induce the rapid generation of the same polyunsaturated diacylglycerols.

    abstract::Prostaglandin F2alpha, platelet-derived growth factor (PDGF) and calcium ionophore A23187 stimulated the rapid (within 25 s) generation of polyunsaturated 1,2-diacylglycerol (DAG) species, in particular 18:0/20:3n-9, 18:0/20:4n-6 and 18:0/20:5n-3, in Swiss 3T3 fibroblasts. This was followed by a second sustained phase...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pettitt TR,Wakelam MJ

    更新日期:1998-05-15 00:00:00

  • Direct reprogramming of terminally differentiated B cells into erythroid lineage.

    abstract::Hematopoietic progenitors have been shown to retain plasticity and switch lineages by appropriate stimuli. However, mature blood cells hardly showed such differentiation plasticity. In this paper, we tried to reprogram mature B cells into erythroid lineage by expressing various hematopoietic transcription factors. Amo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sadahira K,Fukuchi Y,Kunimono H,Sakurai M,Ikeda Y,Okamoto S,Nakajima H

    更新日期:2012-10-19 00:00:00

  • Complete amino acid sequence of fetal bovine serum acetylcholinesterase and its comparison in various regions with other cholinesterases.

    abstract::The complete amino acid sequence of a mammalian acetylcholinesterase from fetal bovine serum (FBS AChE) is presented. This enzyme has a high degree of sequence identity with other cholinesterases, liver carboxyesterases, esterase-6, lysophospholipase, and thyroglobulin. The locations of 191 amino acids in 10 regions o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Doctor BP,Chapman TC,Christner CE,Deal CD,De La Hoz DM,Gentry MK,Ogert RA,Rush RS,Smyth KK,Wolfe AD

    更新日期:1990-06-18 00:00:00

  • Sequence analysis of the promoter region of the rat vasopressin gene.

    abstract::The vasopressin gene is highly transcribed in magnocellular neurons of the supraoptic (SON) and paraventricular nucleus (PVN) in the rat hypothalamus. In order to identify cis-acting elements involved in the expression of the vasopressin gene, approximately 1 kb upstream of the transcription start site has been sequen...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mohr E,Richter D

    更新日期:1990-01-29 00:00:00

  • Leader sequence of a plant ribosomal protein gene with complementarity to the 18S rRNA triggers in vitro cap-independent translation.

    abstract::Cap-independent translation (CIT) occurs at the leader sequences of uncapped plant viral RNAs, but also at a number of normally capped cellular mRNAs and has been correlated with sequence complementarity to 18S rRNA. The ribosomal protein S18 (RPS18) is a component of the small ribosomal subunit and is encoded by thre...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vanderhaeghen R,De Clercq R,Karimi M,Van Montagu M,Hilson P,Van Lijsebettens M

    更新日期:2006-05-15 00:00:00

  • Differential activation by fMet-Leu-Phe and phorbol ester of a plasma membrane phosphatidylcholine-specific phospholipase D in human neutrophil.

    abstract::Signal transduction involving phosphatidylcholine hydrolysis has been investigated in human neutrophils (PMN) after in situ generation of [3H]alkylacyl-sn-glycero-3-phosphocholine ([3H]alkylacyl-GPC) by cell incubation with [3H]alkylacetyl-GPC. When PMN were stimulated with the chemotactic peptide N-formyl-Met-Leu-Phe...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gelas P,Ribbes G,Record M,Terce F,Chap H

    更新日期:1989-07-17 00:00:00

  • Stopped-flow spectrophotometry monitoring the initial processes of platelet activation by platelet-activating factor.

    abstract::The initial processes of platelet activation by platelet-activating factor (PAF) were observed by stopped-flow light scattering and fluorometry. The binding of PAF to rabbit platelets did not change the membrane fluidity, though it caused the removal of calcium from internal stores and induced concomitantly platelet s...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ohga Y,Nakanishi M

    更新日期:1986-10-20 00:00:00

  • Amino acid residue: is it structural or functional?

    abstract::A new approach is suggested for delineating the structural and functional amino acid residues in proteins with known three-dimensional structure, basing on the involvement of residues in intramolecular hydrophobic and hydrophilic interactions and additional information about the conservativity of the residues. The app...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Golovanov AP,Efremov RG,Jaravine VA,Vergoten G,Arseniev AS

    更新日期:1995-11-13 00:00:00

  • Empirical free energy as a target function in docking and design: application to HIV-1 protease inhibitors.

    abstract::Structure-based drug design requires the development of efficient computer programs for exploring the structural compatibility of various flexible ligands with a given receptor. While various algorithms are available for finding docked conformations, selecting a target function that can reliably score the conformation...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: King BL,Vajda S,DeLisi C

    更新日期:1996-04-08 00:00:00

  • Inositol(1,3,4,5) tetrakisphosphate plays an important role in calcium mobilization from Entamoeba histolytica.

    abstract::Calcium release from internal stores of Entamoeba histolytica, a parasitic protozoan, was observed by measuring fluorescence of Fura-2. Emptying of inositol(1,4,5)trisphosphate (Ins(1,4,5)P3)-sensitive calcium pools in permeabilized E. histolytica did not significantly affect subsequent calcium release by inositol(1,3...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Raha S,Giri B,Bhattacharyya B,Biswas BB

    更新日期:1995-04-10 00:00:00

  • Selective inhibition of 5-lipoxygenase by natural compounds isolated from Chinese plants, Artemisia rubripes Nakai.

    abstract::Three of four natural compounds, which are caffeic acid, eupatilin and 4'-demethyleupatilin, isolated from Chinese plant, Artemisia rubripes Nakai selectively inhibited 5-lipoxygenase of cultured mastocytoma cells. Half-inhibition doses (ID50) for caffeic acid, eupatilin and 4'-demethyleupatilin were 3.7, 14 and 18 X ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Koshihara Y,Neichi T,Murota S,Lao A,Fujimoto Y,Tatsuno T

    更新日期:1983-07-11 00:00:00

  • A light-sensitive mechanism differently regulates transcription and transcript stability of omega3 fatty-acid desaturases (FAD3, FAD7 and FAD8) in soybean photosynthetic cell suspensions.

    abstract::The omega3 fatty-acid desaturases: FAD7 and FAD8 (plastid) and FAD3 (reticular) are responsible for trienoic fatty-acid (TA) production in plants. The expression of these enzymes seemed to be regulated differently in response to light. Darkness leads to a decrease in total TA level. Under such conditions, FAD3 and FAD...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Collados R,Andreu V,Picorel R,Alfonso M

    更新日期:2006-09-04 00:00:00

  • LncRNA FENDRR attenuates adriamycin resistance via suppressing MDR1 expression through sponging HuR and miR-184 in chronic myelogenous leukaemia cells.

    abstract::Chemotherapy is a major anticancer therapeutic modality, however, multidrug resistance (MDR) is frequently observed and hinders treatment efficacy. Here, we investigated the role and potential mechanism of the long noncoding RNA (lncRNA) FENDRR in adriamycin resistance of chronic myeloid leukaemia (CML) cells. FENDRR ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zhang F,Ni H,Li X,Liu H,Xi T,Zheng L

    更新日期:2019-08-01 00:00:00

  • The verprolin family of proteins: regulators of cell morphogenesis and endocytosis.

    abstract::The verprolin family of proteins, WIP, CR16 and WIRE/WICH, has emerged as critical regulators of cytoskeletal organisation in vertebrate cells. The founding father of the family, verprolin, was originally identified in budding yeast and later shown to be needed for actin polymerisation during polarised growth and duri...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Aspenström P

    更新日期:2005-10-10 00:00:00

  • 31P NMR as a tool for monitoring detergent solubilization of sarcoplasmic reticulum membranes.

    abstract::Sarcoplasmic reticulum vesicles were solubilized stepwise by the nonionic detergent octaethyleneglycol monododecyl ether; 31P NMR enabled the extent of phospholipid solubilization to be monitored by following the conversion of the broad resonance peak characterizing the phospholipids inserted in the bilayer to the nar...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Roux M,Champeil P

    更新日期:1984-06-11 00:00:00

  • Ground state isomerization of a model green fluorescent protein chromophore.

    abstract::The relationship between ground state cis-trans isomerization and protonation state is explored for a model green fluorescent protein chromophore, 4-hydroxybenzylidene-1,2-dimethylimidazolinone (HBDI). We find that the protonation state has only a modest effect on the free energy differences between cis and trans isom...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: He X,Bell AF,Tonge PJ

    更新日期:2003-08-14 00:00:00

  • Interaction of avidin with spin-labelled N-biotinyl phosphatidylethanolamine in a lipid membrane.

    abstract::N-Biotinyl phosphatidylethanolamine spin labelled at the C-14 position of the sn-2 chain has been incorporated at a level of 1 mol% in bilayers of dimyristoyl phosphatidylcholine, and the effects on the chain mobility of binding avidin to the biotin lipid headgroup have been studied by electron spin resonance spectros...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Swamy MJ,Marsh D

    更新日期:1993-06-07 00:00:00

  • TGF-β triggers HBV cccDNA degradation through AID-dependent deamination.

    abstract::The covalently closed circular DNA (cccDNA) of hepatitis B virus (HBV) is a viral center molecule for HBV infection and persistence. However, the cellular restriction factors of HBV cccDNA are not well understood. Here, we show that TGF-β can induce nuclear viral cccDNA degradation and hypermutation via activation-ind...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Qiao Y,Han X,Guan G,Wu N,Sun J,Pak V,Liang G

    更新日期:2016-02-01 00:00:00

  • Conserved recoding RNA editing of vertebrate C1q-related factor C1QL1.

    abstract::A-to-I RNA editing can lead to recoding of pre-mRNAs with profound functional consequences for the ensuing proteins. Here we show that complement component 1, q subcomponent-like 1 (C1QL1) undergoes RNA editing in vivo causing non-synonymous amino acid substitutions in human, mouse as well as zebrafish. The major edit...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sie CP,Maas S

    更新日期:2009-04-02 00:00:00

  • A new modified DNA enzyme that targets influenza virus A mRNA inhibits viral infection in cultured cells.

    abstract::DNA enzymes are RNA-cleaving single-stranded DNA molecules. We designed DNA enzymes targeting the PB2 mRNA translation initiation (AUG) region of the influenza A virus (A/PR/8/34). The modified DNA enzymes have one or two N3'-P5' phosphoramidate bonds at both the 3'- and 5'-termini of the oligonucleotides, which signi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Takahashi H,Hamazaki H,Habu Y,Hayashi M,Abe T,Miyano-Kurosaki N,Takaku H

    更新日期:2004-02-27 00:00:00

  • Quantitative study of the binding of cysteine proteinases to basement membranes.

    abstract::Binding of cysteine proteinases of the papain superfamily (papain and cathepsins B, B-like and L) to basement membranes was studied by using the enzymatic activity of these proteinases against their specific fluorogenic substrates. Papain inactivated by E64 was used for Kd determination by competition experiments. The...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Guinec N,Dalet-Fumeron V,Pagano M

    更新日期:1992-08-24 00:00:00

  • A mutational analysis of active site residues in trans-3-chloroacrylic acid dehalogenase.

    abstract::trans-3-Chloroacrylic acid dehalogenase (CaaD) catalyzes the hydrolytic dehalogenation of trans-3-haloacrylates to yield malonate semialdehyde by a mechanism utilizing βPro-1, αArg-8, αArg-11, and αGlu-52. These residues are implicated in a promiscuous hydratase activity where 2-oxo-3-pentynoate is processed to acetop...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Poelarends GJ,Serrano H,Huddleston JP,Johnson WH Jr,Whitman CP

    更新日期:2013-09-02 00:00:00