Structural modifications associated with the change in Ca2+ sensitivity on activation of m-calpain.

abstract：:The gene for human tetranectin was isolated from a genomic library with a mixture of degenerate oligonucleotide probes. The gene is about 12 kbp and contains two intervening sequences. The gene encodes a protein of 202 amino acid residues, with a signal peptide of 21 amino acid residues, followed by the tetranectin se...

journal_title：FEBS letters

authors： Berglund L,Petersen TE

更新日期：1992-08-31 00:00:00

abstract：:Rho GTPases participate in various important signaling pathways and have been implicated in myogenic differentiation. Here the first evidence is provided that in C2C12 myoblasts sphingosine 1-phosphate (SPP) rapidly and transiently induced membrane association of Rho A in a pertussis toxin-insensitive manner. The bioa...

journal_title：FEBS letters

authors： Meacci E,Donati C,Cencetti F,Romiti E,Bruni P

更新日期：2000-09-29 00:00:00

abstract：:Bovine tryptophanyl-tRNA synthetase (TrpRS, E.C.6.1.1.2) is unable to catalyze in vitro formation of Ap4A in contrast to some other aminoacyl-tRNA synthetases. However, in the presence of L-tryptophan, ATP-Mg2+ and ADP the enzyme catalyzes the Ap3A synthesis via adenylate intermediate. Ap3A (not Ap4A) may serve as a s...

journal_title：FEBS letters

authors： Merkulova T,Kovaleva G,Kisselev L

更新日期：1994-08-22 00:00:00

abstract：:Aspergillus nidulans catalase B (CatB) was purified to homogeneity and characterized as a hydroperoxidase which resembles typical catalases in some physicochemical characteristics: (1) it has an apparent molecular weight of 360000 and is composed of four glycosylated subunits, (2) it has hydrophobic properties as reve...

journal_title：FEBS letters

authors： Calera JA,Sánchez-Weatherby J,López-Medrano R,Leal F

更新日期：2000-06-16 00:00:00

abstract：:The thermal blockage of QA-. oxidation was analysed in PS II membrane fragments by monitoring flash-induced changes of the relative fluorescence quantum yield as a function of temperature. The results obtained reveal: (a) in dark-adapted samples the fraction of QA-. that is not reoxidised within a time domain of 10 s ...

journal_title：FEBS letters

authors： Reifarth F,Renger G

更新日期：1998-05-29 00:00:00

abstract：:Selective protein degradation is an energy-dependent process performed by high-molecular-weight proteases. The activity of proteolytic components of these enzymes is coupled to the ATPase activity of their regulatory subunits or domains. Here, we obtained the proteolytic domain of Escherichia coli protease Lon by clon...

journal_title：FEBS letters

authors： Rasulova FS,Dergousova NI,Starkova NN,Melnikov EE,Rumsh LD,Ginodman LM,Rotanova TV

更新日期：1998-08-07 00:00:00

abstract：:The antigenic cross-reactivity between purified chick, eel and mouse electrolectins (endogenous beta-D-galactoside specific lectins) have been studied using a solid phase radioimmunoassay. The immune serum raised against the eel electrolectin crossreacts both with the chick and the mouse electrolectins, while the anti...

journal_title：FEBS letters

authors： Levi G,Teichberg VI

更新日期：1982-11-01 00:00:00

abstract：:We present a new water selective pulse sequence allowing rapid determination of exchange rates of labile protons on the millisecond time scale. Using diffusion measurements, exchange rates of resolved protons can be determined without prior knowledge of relaxation parameters in a short overnight experiment. The use of...

journal_title：FEBS letters

authors： Böckmann A,Guittet E

更新日期：1997-11-24 00:00:00

abstract：:Snake venoms are rich sources of serine proteinase inhibitors that are members of the Kunitz/BPTI (bovine pancreatic trypsin inhibitor) family. However, only a few of their gene sequences have been determined from snakes. We therefore cloned the cDNAs for the trypsin and chymotrypsin inhibitors from a Vipera ammodytes...

journal_title：FEBS letters

authors： Zupunski V,Kordis D,Gubensek F

更新日期：2003-07-17 00:00:00

abstract：:A variety of plant tissues contain an uncoupling mitochondrial protein (PUMP), recently described and characterized by our group. In this study we show that the inhibition of PUMP activity in potato tuber mitochondria significantly increases mitochondrial H2O2 generation, while PUMP substrates, such as linoleic acid, ...

journal_title：FEBS letters

authors： Kowaltowski AJ,Costa AD,Vercesi AE

更新日期：1998-03-27 00:00:00

abstract：:The addition of a poly-His C-terminal extension, designed to facilitate the purification of the protein, to the beta-lactamase of a thermophilic Bacillus licheniformis strain modified the site of action of the signal peptidase. This resulted in the secretion of a protein with a different N-terminus, showing that this ...

journal_title：FEBS letters

authors： Ledent P,Duez C,Vanhove M,Lejeune A,Fonzé E,Charlier P,Rhazi-Filali F,Thamm I,Guillaume G,Samyn B,Devreese B,Van Beeumen J,Lamotte-Brasseur J,Frère JM

更新日期：1997-08-18 00:00:00

abstract：:The use of tobacco plants as a novel expression system for the production of human homotrimeric collagen I is presented in this report. Constructs were engineered from cDNA encoding the human proalpha1(I) chain to generate transgenic tobacco plants expressing collagen I. The recombinant proalpha1(I) chains were expres...

journal_title：FEBS letters

authors： Ruggiero F,Exposito JY,Bournat P,Gruber V,Perret S,Comte J,Olagnier B,Garrone R,Theisen M

更新日期：2000-03-03 00:00:00

abstract：:Glycogen phosphorylase b at concentrations close to those found in skeletal muscle interacts with sarcoplasmic reticulum membranes, but not with liposomes made of lipids extracted from these membranes, and is inhibited upon binding to the membrane. The interaction of glycogen phosphorylase with the sarcoplasmic reticu...

journal_title：FEBS letters

authors： Cuenda A,Centeno F,Gutierrez-Merino C

更新日期：1991-06-03 00:00:00

abstract：:The mitochondrial alternative oxidase is a diiron carboxylate quinol oxidase (Dox) found in plants and some fungi and protists, but not animals. The plastid terminal oxidase is distantly related to alternative oxidase and is most likely also a Dox protein. Database searches revealed that the alpha-proteobacterium Novo...

journal_title：FEBS letters

authors： Finnegan PM,Umbach AL,Wilce JA

更新日期：2003-12-18 00:00:00

abstract：:Cellular homeostasis is linked tightly to mitochondrial functions. Some damage to mitochondrial proteins and nucleic acids can lead to the depolarization of the inner mitochondrial membrane, thereby sensitizing impaired mitochondria for selective elimination by autophagy. Mitochondrial dysfunction is one of the key as...

journal_title：FEBS letters

authors： Tanaka A

更新日期：2010-04-02 00:00:00

abstract：:The inhibition of electron transport by unnatural (-)-antimycin A3 was examined with rat liver mitochondria and compared with that of natural (+)-antimycin A3. (-)-Antimycin A3 inhibited respiration about 1/100th as strongly as natural (+)-antimycin A3. (-)-Antimycin A3 binding to the cytochrome bc1 complex did not se...

journal_title：FEBS letters

authors： Miyoshi H,Kondo H,Oritani T,Saitoh I,Iwamura H

更新日期：1991-11-04 00:00:00

abstract：:HGTD-P is a hypoxia-responsive pro-apoptotic protein that transmits hypoxic signals directly to mitochondria. When overexpressed, HGTD-P induces cell death via typical mitochondrial apoptotic cascades. However, much is unknown about post-transcriptional modification and signaling networks of HGTD-P in association with...

journal_title：FEBS letters

authors： Kim JY,Kim SM,Ko JH,Yim JH,Park JH,Park JH

更新日期：2006-05-29 00:00:00

abstract：:Cav3.2 T-type Ca(2+) channel is required for the activation of calcineurin/NFAT signaling in cardiac hypertrophy. We aimed to investigate how Cav3.2 and calcineurin interact. We found that Ca(2+) and calmodulin modulate the Cav3.2/calcineurin interaction. Calcineurin binding to Cav3.2 decreases the enzyme's phosphatas...

journal_title：FEBS letters

authors： Huang CH,Chen YC,Chen CC

更新日期：2013-06-19 00:00:00

abstract：:The regulation of genes for acetylcholine receptor (AChR), myogenic factors and other muscle-specific proteins has been analyzed in experimental autoimmune myasthenia gravis (EAMG) and following denervation. The levels of the transcripts for the myogenic factors, MyoD1, myogenin and MRF4, were measured using Northern ...

journal_title：FEBS letters

authors： Asher O,Fuchs S,Zuk D,Rapaport D,Buonanno A

更新日期：1992-03-24 00:00:00

abstract：:Bacterial fructosyltransferase enzymes belonging to glycoside hydrolase family 68 (GH68) are not known to require a metal cofactor. Here, we show that Ca2+ ions play an important structural role in the Lactobacillus reuteri 121 levansucrase (Lev) and inulosucrase (Inu) enzymes. Analysis of the Bacillus subtilis Lev 3D...

journal_title：FEBS letters

authors： Ozimek LK,Euverink GJ,van der Maarel MJ,Dijkhuizen L

更新日期：2005-02-14 00:00:00

abstract：:The cyclic AMP phosphodiesterases type 4 (PDE4s) are expressed in a cell specific manner, with intracellular targeting directed by unique N-terminal anchor domains. All long form PDE4s are phosphorylated and activated by PKA phosphorylation within their upstream conserved region 1 (UCR1). Here, we identify and charact...

journal_title：FEBS letters

authors： Byrne AM,Elliott C,Hoffmann R,Baillie GS

更新日期：2015-03-12 00:00:00

abstract：:The carboxy-terminal half of the c-src protein fused to the protein A moiety was expressed in bacteria. The protein A/truncated c-src fusion protein, which does not have SH2 and SH3 domains, is found in the periplasmic space allowing for a simple one-step purification and demonstrated high efficiency in autophosphoryl...

journal_title：FEBS letters

authors： Saya H,Lee PS,Nishi T,Izawa I,Nakajima M,Gallick GE,Levin VA

更新日期：1993-07-26 00:00:00

abstract：:Insertion and deletion analyses of a protein have been less common than point mutation analyses, partly due to the lack in effective methods. This is the case with the green fluorescent protein (GFP), which is so widely applied in molecular biology and other fields. In this paper we first introduce a systematic approa...

journal_title：FEBS letters

authors： Kitamura K,Yoshida C,Nishigaki K

更新日期：2003-12-18 00:00:00

abstract：:In Ruminococcus albus, 4-O-β-D-mannosyl-D-glucose phosphorylase (RaMP1) and β-(1,4)-mannooligosaccharide phosphorylase (RaMP2) belong to two subfamilies of glycoside hydrolase family 130. The two enzymes phosphorolyze β-mannosidic linkages at the nonreducing ends of their substrates, and have substantially diverse sub...

journal_title：FEBS letters

authors： Ye Y,Saburi W,Odaka R,Kato K,Sakurai N,Komoda K,Nishimoto M,Kitaoka M,Mori H,Yao M

更新日期：2016-03-01 00:00:00

abstract：:Polypeptides rich in glutamic acid are strong inhibitors purified from isolated nuclei of Xenopus laevis oocytes of casein kinase II. The presence of tyrosine in these peptides greatly enhances their inhibitory capacity. Using casein as a substrate, copolyglu:tyr (4:1) has an I50 value of 20 nM, 250 fold lower than th...

journal_title：FEBS letters

authors： Tellez R,Gatica M,Allende CC,Allende JE

更新日期：1990-06-04 00:00:00

abstract：:We have demonstrated over a decade ago that hepoxilins cause the release of insulin from isolated pancreatic islets of Langerhans in vitro. However, no studies are available so far to indicate whether these compounds are active in vivo. The present study is the first to our knowledge which demonstrates that hepoxilins...

journal_title：FEBS letters

authors： Pace-Asciak CR,Demin PM,Estrada M,Liu G

更新日期：1999-11-19 00:00:00

abstract：:An antibody recognising acetylated core histones was used to immunoprecipitate chromatin fragments from proliferating human K562 cells and from cells induced to differentiate with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). The DNA of the acetylated chromatin was probed with sequences of platelet der...

journal_title：FEBS letters

authors： Clayton AL,Hebbes TR,Thorne AW,Crane-Robinson C

更新日期：1993-12-20 00:00:00

abstract：:CXCR2 plays a key role in protecting the integrity of the endothelium. Emerging evidence has demonstrated that the long ncRNAs (lncRNA) Human metastasis associated lung adenocarcinoma transcript 1 (MALAT1) participates in the regulation of the pathophysiological processes. However, whether there is crosstalk between C...

journal_title：FEBS letters

authors： Tang Y,Jin X,Xiang Y,Chen Y,Shen CX,Zhang YC,Li YG

更新日期：2015-10-07 00:00:00

abstract：:Brain-type creatine kinase B-CK (EC 2.7.3.2) was purified from several chicken tissues, e.g. cardiac muscle, brain, gizzard and retina. Two major monomeric chicken B-CK subunits, designated Bb (basic) and Ba (acidic), which differ in isoelectric point, were separated by chromatofocusing in the presence of 8 M urea on ...

journal_title：FEBS letters

authors： Quest AF,Eppenberger HM,Wallimann T

更新日期：1990-03-26 00:00:00

abstract：:The BChla-containing B880-complex (core-complex) of Rhodopseudomonas marina (Rhodospirillaceae) was isolated with a new purification method. The isolation of the B880-complex was performed by solubilisation of the photosynthetic membranes with the detergent LDAO and subsequent fractionated ammonium-sulfate precipitati...

journal_title：FEBS letters

authors： Meckenstock RU,Brunisholz RA,Zuber H

更新日期：1992-10-19 00:00:00