Copolymers of glutamic acid and tyrosine are potent inhibitors of oocyte casein kinase II.


:Polypeptides rich in glutamic acid are strong inhibitors purified from isolated nuclei of Xenopus laevis oocytes of casein kinase II. The presence of tyrosine in these peptides greatly enhances their inhibitory capacity. Using casein as a substrate, copolyglu:tyr (4:1) has an I50 value of 20 nM, 250 fold lower than that of polyglutamic acid which is 5 microM. A similar large difference is observed when a synthetic peptide is used as substrate. The inhibition of copolyglu:tyr is competitive with casein and can be completely reversed by high ionic strength. The relative inhibitory capacity of the polypeptides tested, in descending order, is copolyglu:tyr (4:1) greater than copolyglu:tyr (1:1) greater than polyglu greater than copolyglu:phe (4:1) greater than copolyglu:ala (6:4) greater than copolyglu:leu (4:1). The high affinity for tyrosine-containing acid peptides is shared by rat liver and yeast casein kinase II so that it seems to be a general property of these enzymes.




FEBS letters


Tellez R,Gatica M,Allende CC,Allende JE




Has Abstract


1990-06-04 00:00:00














  • Construction and characterization of the soybean leaf metalloproteinase cDNA.

    abstract::The cloning and analysis of a cDNA clone encoding the soybean metalloproteinase obtained by polymerase chain reaction (PCR) and the rapid amplification of cDNA ends (RACE) reaction are described. The cDNA was constructed from poly(A)+ RNA isolated from 15-17 day old leaves. The deduced amino acid sequence of the cDNA ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pak JH,Liu CY,Huangpu J,Graham JS

    更新日期:1997-03-10 00:00:00

  • Characterization of phospholipase A2 from the venom of Horned viper (Cerastes cerastes).

    abstract::Phospholipase A2 has been purified from the venom of Horned viper (Cerastes cerastes) by gel permeation chromatography followed by reverse-phase HPLC. The primary structure was established by sequence analysis of the intact protein and its enzymic peptides. The structure has 120 residues, properties like other group I...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Siddiqi AR,Shafqat J,Zaidi ZH,Jörnvall H

    更新日期:1991-01-14 00:00:00

  • Adiponectin receptor binding proteins--recent advances in elucidating adiponectin signalling pathways.

    abstract::Adiponectin whose systemic levels are reduced in obesity-related diseases ameliorates insulin sensitivity and regulates biological processes like apoptosis, proliferation, migration and inflammation. Adiponectin binds to adiponectin receptors, AdipoR1 and AdipoR2, which are ubiquitously expressed. Clathrin-dependent e...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Buechler C,Wanninger J,Neumeier M

    更新日期:2010-10-22 00:00:00

  • An isoform of the human calcitonin receptor is expressed in TT cells and in medullary carcinoma of the thyroid.

    abstract::We amplified, using the polymerase chain reaction and calcitonin receptor (CTR) specific primers, RNA extracted from medullary thyroid carcinoma (MTC) and the derived TT cell line. Both secrete large amounts of calcitonin. Electrophoresis of amplification products revealed, in both cases, an ethidium bromide-stained b...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Frendo JL,Pichaud F,Mourroux RD,Bouizar Z,Segond N,Moukhtar MS,Jullienne A

    更新日期:1994-04-04 00:00:00

  • Purification and functional reconstitution of a truncated human Na(+)/glucose cotransporter (SGLT1) expressed in E. coli.

    abstract::A truncated human Na(+)/glucose cotransporter (C(5), residues 407-664) was expressed and purified from Escherichia coli using a GST fusion vector and glutathione affinity chromatography. The truncated transporter (C(5)) was cleaved from GST-C(5) by Factor Xa proteolysis and purified by gel filtration chromatography. U...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Panayotova-Heiermann M,Leung DW,Hirayama BA,Wright EM

    更新日期:1999-10-15 00:00:00

  • Cloning and sequencing of the bacterioferritin gene of Brucella melitensis 16M strain.

    abstract::The 40 N-terminal amino acids of the 20 kDa antigen A2 from Brucella melitensis were sequenced and showed important similarities with 4 bacterioferrins. A monoclonal antibody raised against this antigen cross-reacted with Escherichia coli bacterioferritin. Hybridization of two sets of degenerate primers with B. melite...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Denoel PA,Zygmunt MS,Weynants V,Tibor A,Lichtfouse B,Briffeuil P,Limet JN,Letesson JJ

    更新日期:1995-03-20 00:00:00

  • Cloning and expression of a bovine adenylyl cyclase type VII specific to the retinal pigment epithelium.

    abstract::A cDNA of a type 7 adenylyl cyclase isoform was cloned from a bovine retinal pigment epithelium cDNA library using oligonucleotides developed to conserved regions common to mammalian adenylyl cyclases. A 6.7 kb mRNA of very high abundance was uniquely present on Northern blots containing mRNA or total RNA from the pig...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Völkel H,Beitz E,Klumpp S,Schultz JE

    更新日期:1996-01-15 00:00:00

  • Cooperativity and flexibility of active sites in homodimeric transketolase.

    abstract::Here we summarize evidence for non-equivalence of two structurally similar active sites in transketolase and other thiamine-dependent enzymes. This non-equivalence takes place when the enzymes interact with various ligands (inhibitors, cations, coenzyme and substrates). Data on different strains in the structure of th...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kovina MV,Kochetov GA

    更新日期:1998-11-27 00:00:00

  • Intercellular communication between vascular smooth muscle and endothelial cells mediated by heparin-binding epidermal growth factor-like growth factor and vascular endothelial growth factor.

    abstract::Heparin-binding epidermal growth factor-like growth factor (HB-EGF), a potent mitogen and migration factor for vascular smooth muscle cells (SMC), promoted neovascularization in vivo in the rabbit cornea. MRI demonstrated quantitatively the angiogenic effect of HB-EGF when introduced subcutaneously into nude mice. HB-...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Abramovitch R,Neeman M,Reich R,Stein I,Keshet E,Abraham J,Solomon A,Marikovsky M

    更新日期:1998-04-03 00:00:00

  • Vacuolation induced by cytotoxin from Helicobacter pylori is mediated by the EGF receptor in HeLa cells.

    abstract::The bacterial toxin VacA produced by H. pylori induces large vacuoles in several types of cultured cells such as HeLa cells or gastric cells. To determine the mechanism of vacuolation induced by this toxin we employed several inhibitors of membrane trafficking and endocytosis. The development of vacuolation induced by...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Seto K,Hayashi-Kuwabara Y,Yoneta T,Suda H,Tamaki H

    更新日期:1998-07-24 00:00:00

  • Evolution of dnaQ, the gene encoding the editing 3' to 5' exonuclease subunit of DNA polymerase III holoenzyme in Gram-negative bacteria.

    abstract::The nucleotide sequences of the dnaQ genes from Salmonella typhimurium and Buchnera aphidicola, encoding the epsilon-subunit of the DNA polymerase III holoenzyme, have been determined. The Salmonella typhimurium dnaQ protein consists of 243 amino acid residues with a calculated molecular weight of 27224. The Buchnera ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Huang Y,Braithwaite DK,Ito J

    更新日期:1997-01-02 00:00:00

  • Cell cycle-dependent association of Gag-Mil and hsp90.

    abstract::Immunoprecipitated p100Gag-Mil protein kinase from MH2-transformed quail embryo fibroblasts is associated with an 89 kDa protein. The molar ratio between p89 and Gag-Mil in the immunocomplex is 0.72, indicating that the majority of Gag-Mil is complexed with p89. During mitosis part of Gag-Mil is shifted to a form with...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lovrić J,Bischof O,Moelling K

    更新日期:1994-04-18 00:00:00

  • The kinetics of the interaction between the actin-binding domain of alpha-actinin and F-actin.

    abstract::Measurement of the binding equilibrium for the interaction of alpha-actinin with F-actin is complicated by secondary reactions involving cross-linking and/or bundling of the actin filaments. To quantitate the initial binding event, we studied the interaction of the bacterially expressed actin-binding domain (ABD) of c...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kuhlman PA,Ellis J,Critchley DR,Bagshaw CR

    更新日期:1994-02-21 00:00:00

  • Mobilization of arachidonic acid between diacyl and ether phospholipids in rabbit alveolar macrophages.

    abstract::The metabolism of 20:4 (arachidonic acid) in alkenylacyl, alkylacyl and diacyl lipid classes in choline glycerophospholipids (CGP) and ethanolamine glycerophospholipids (EGP) in rabbit alveolar macrophages was examined. [3H]20:4 was very rapidly incorporated into diacyl glycerophosphocholine (GPC). After the removal o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sugiura T,Katayama O,Fukui J,Nakagawa Y,Waku K

    更新日期:1984-01-09 00:00:00

  • Functional relevance of the internal hydrophobic cavity of urate oxidase.

    abstract::Urate oxidase from Aspergillus flavus is a 135 kDa homo-tetramer which has a hydrophobic cavity buried within each monomer and located close to its active site. Crystallographic studies under moderate gas pressure and high hydrostatic pressure have shown that both gas presence and high pressure would rigidify the cavi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Colloc'h N,Prangé T

    更新日期:2014-05-02 00:00:00

  • Y1 and Y2 receptors for neuropeptide Y.

    abstract::By using monoiodinated radioligands of both intact neuropeptide Y (NPY) and of a long C-terminal fragment, NPY13-36, two subtypes of binding sites, which differ in affinity and specificity, have been characterized. The Y1 type of binding site, characterized on a human neuroblastoma cell line, MC-IXC, and a rat pheochr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sheikh SP,Håkanson R,Schwartz TW

    更新日期:1989-03-13 00:00:00

  • Role of mitochondria and C-terminal membrane anchor of Bcl-2 in Bax induced growth arrest and mortality in Saccharomyces cerevisiae.

    abstract::In mammalian cells, the Bcl-2 and Bcl-x(L) proteins suppress programmed cell death whereas the topographically similar Bax protein accelerates the apoptotic process. Recently published data suggest that expression of the human Bax-alpha gene is lethal for the yeast Saccharomyces cerevisiae and that this toxicity can b...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Greenhalf W,Stephan C,Chaudhuri B

    更新日期:1996-02-12 00:00:00

  • Single and cell population respiratory oscillations in yeast: a 2-photon scanning laser microscopy study.

    abstract::Two-photon scanning laser and confocal microscopies were used to image metabolic dynamics of single or cell populations of Saccharomyces cerevisiae strain 28033. Autofluorescence of reduced nicotinamide nucleotides, and mitochondrial membrane potential (DeltaPsim), were simultaneously monitored. Spontaneous, large-sca...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Aon MA,Cortassa S,Lemar KM,Hayes AJ,Lloyd D

    更新日期:2007-01-09 00:00:00

  • Crystallographic analysis of the intact metal centres [3Fe-4S](1+/0) and [4Fe-4S](2+/1+) in a Zn(2+) -containing ferredoxin.

    abstract::Detailed structural models of di-cluster seven-iron ferredoxins constitute a valuable resource for folding and stability studies relating the metal cofactors' role in protein stability. The here reported, hemihedric twinned crystal structure at 2.0 A resolution from Acidianus ambivalens ferredoxin, shows an integral 1...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Frazão C,Aragão D,Coelho R,Leal SS,Gomes CM,Teixeira M,Carrondo MA

    更新日期:2008-03-05 00:00:00

  • Kinetics of the interaction of endotoxin with polymyxin B and its analogs: a surface plasmon resonance analysis.

    abstract::Lipopolysaccharide, the invariant structural component of Gram-negative bacteria, when present in minute amounts in the circulation in humans elicits 'endotoxic shock' syndrome, which is fatal in 60% of the cases. Polymyxin B (PMB), a cyclic cationic peptide, neutralizes the endotoxin, but also induces many harmful si...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Thomas CJ,Surolia A

    更新日期:1999-02-26 00:00:00

  • Crystallization of cytochrome P-450scc from bovine adrenocortical mitochondria.

    abstract::Cytochrome P-450scc (P-450scc), a cholesterol side-chain cleavage enzyme from bovine adrenocortical mitochondria, has been crystallized for the first time. Upon removal of glycerol from the solution of the native enzyme complexed with pyridoxal 5'-phosphate (PLP) by microdialysis against distilled water, reddish and p...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Iwamoto Y,Tsubaki M,Hiwatashi A,Ichikawa Y

    更新日期:1988-06-06 00:00:00

  • Expression of CYP2S1 in human hepatic stellate cells.

    abstract::Activated stellate cells are myofibroblast-like cells associated with the generation of fibrotic scaring in chronically damaged liver. Gene chip analysis was performed on cultured fibrotic stellate cells. Of the 51 human CYP genes known, 13 CYP and 5 CYP reduction-related genes were detected with 4 CYPs (CYP1A1, CYP2E...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Marek CJ,Tucker SJ,Koruth M,Wallace K,Wright MC

    更新日期:2007-02-20 00:00:00

  • Sphingolipid topology and the dynamic organization and function of membrane proteins.

    abstract::When acquiring internal membranes and vesicular transport, eukaryotic cells started to synthesize sphingolipids and sterols. The physical differences between these and the glycerophospholipids must have enabled the cells to segregate lipids in the membrane plane. Localizing this event to the Golgi then allowed them to...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: van Meer G,Hoetzl S

    更新日期:2010-05-03 00:00:00

  • Insulin-dependent translocation of the small GTP-binding protein rab3C in cardiac muscle: studies on insulin-resistant Zucker rats.

    abstract::The failure of insulin-regulated recruitment of the GLUT4 glucose transporter in cardiac muscle of obese Zucker rats is associated with alterations of the subcellular distribution of the small-molecular-mass GTP-binding protein rab4A. Here, we show by subcellular fractionation and Western blotting a translocation of t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Uphues I,Chern Y,Eckel J

    更新日期:1995-12-18 00:00:00

  • Expression of the vasopressin and oxytocin genes in human hypothalami.

    abstract::Poly(A)+ RNA isolated from post-mortem human hypothalami has been used to characterize the poly-protein precursors to vasopressin and oxytocin. Translation in a cell-free system and subsequent immuno-precipitation with antibodies raised against either vasopressin or neurophysin identified a product of Mr 19000 (prepro...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mohr E,Hillers M,Ivell R,Haulica ID,Richter D

    更新日期:1985-11-25 00:00:00

  • Stable insertion of Alzheimer Abeta peptide into the ER membrane strongly correlates with its length.

    abstract::Alzheimer's disease is characterized by the deposition of amyloid beta-peptide (Abeta) plaques in the brain. Full-length amyloid-beta precursor protein (APP) is processed by alpha- and beta-secretases to yield soluble APP derivatives and membrane-bound C-terminal fragments, which are further processed by gamma-secreta...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lundin C,Johansson S,Johnson AE,Näslund J,von Heijne G,Nilsson I

    更新日期:2007-08-07 00:00:00

  • Three-dimensional structure and antigenicity of transmembrane-protein peptides of the human immunodeficiency virus type 1. Effects of a neutralization-escape substitution.

    abstract::A point mutation (Ala-589 to Thr) in the transmembrane protein of the human immunodeficiency virus type 1 (HIV-1) has been shown to decrease the sensitivity of the virus to the neutralizing effect of human HIV-1 specific antibodies [(1990) J. Virol. 64, 3240-3248]. Here 17-residue peptides with the parental and mutant...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Klasse J,Pipkorn R,Blomberg J,Han KH,Hilton B,Ferretti JA

    更新日期:1993-05-24 00:00:00

  • Site-directed mutagenesis of alanine-382 of human antithrombin III.

    abstract::Antithrombin III Hamilton is a structural variant of antithrombin III (AT-III) with normal heparin affinity but impaired serine protease inhibitory activity. The molecular defect of AT-III-Hamilton is a substitution of threonine for alanine at amino acid residue 382. Recently it has been shown that both plasma-derived...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Austin RC,Rachubinski RA,Blajchman MA

    更新日期:1991-03-25 00:00:00

  • Persistent activity modification of phosphatidate phosphohydrolase and fatty acyl-CoA synthetase on incubation of adipocytes with the tumour promoter 12-O-tetradecanoylphorbol 13-acetate.

    abstract::Incubation of rat adipocytes with the phorbol ester TPA caused a dose-dependent, persistent increase in phosphatidate phosphohydrolase activity and decrease in fatty acyl-CoA synthetase activity. Half-maximal effects were obtained with 1-2 X 10(-10) M TPA. TPA did not alter basal or noradrenaline-stimulated lipolysis....

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hall M,Taylor SJ,Saggerson ED

    更新日期:1985-01-07 00:00:00

  • Hepatitis B virus X increases immune cell recruitment by induction of chemokine SDF-1.

    abstract::Hepatitis B virus X protein is a major factor in the HBV-induced disease developments. Stromal cell-derived factor-1 is a small cytokine that is strongly chemotactic for lymphocytes. We explored the role of HBx on recruitment of HBV-induced virus-nonspecific immune cells into liver. Immune cell recruitment and SDF-1 e...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cho HK,Kim SY,Seong JK,Cheong J

    更新日期:2014-03-03 00:00:00