Copolymers of glutamic acid and tyrosine are potent inhibitors of oocyte casein kinase II.

abstract：:Mitochondrial ATP synthase, a major ATP supplier in respiring cells, should be regulated in amount and in activity to respond to the varying demands of cells for ATP. We screened 80 protein kinase inhibitors and found that HeLa cells treated with four inhibitors exhibited reduced mitochondrial ATP synthesis activity. ...

journal_title：FEBS letters

authors： Sugawara K,Fujikawa M,Yoshida M

更新日期：2013-11-29 00:00:00

abstract：:We analyzed the expression of aquaporins (AQPs) in the small intestine to elucidate their functions, and found that AQP9, which had not previously been detected there, is present in duodenum, jejunum, and ileum. AQP9 is expressed in colon as well, but not in stomach. Also, its expression in these intestinal sections i...

journal_title：FEBS letters

authors： Okada S,Misaka T,Matsumoto I,Watanabe H,Abe K

更新日期：2003-04-10 00:00:00

abstract：:Stimulation of mas-oncogene transfected 401L-C3 cells by angiotensins leads to the production of inositol phosphates. This response shows dose dependence, and has an apparent rank order of potency angiotensin III greater than or equal to angiotensin II much greater than angiotensin I. Preincubation with 12-O-tetradeca...

journal_title：FEBS letters

authors： Jackson TR,Hanley MR

更新日期：1989-07-17 00:00:00

abstract：:To search for negative regulatory components of the NF-kappaB activation pathways, we mutagenized Rat-1 fibroblasts and established a stable mutant cell line with a constitutive NF-kappaB activity. This mutant cell line, designated as TK26, showed permanently elevated I kappa B kinase (IKK) activity and a genetically ...

journal_title：FEBS letters

authors： Chinanonwait N,Miura H,Yamamoto N,Yamaoka S

更新日期：2002-11-20 00:00:00

abstract：:We have studied the pharmacological properties of genetically engineered human NK1 tachykinin receptors in which residues at the extracellular surface of the fourth transmembranal domain were substituted with the corresponding amino acids from the NK2 receptor. We show that substitution of G166C:Y167F in the human NK1...

journal_title：FEBS letters

authors： Ciucci A,Palma C,Riitano D,Manzini S,Werge TM

更新日期：1997-10-27 00:00:00

abstract：:Proteorhodopsin (PR), a retinal-containing seven transmembrane helix protein, functions as a light-driven proton pump. Using PCR, we isolated 18 PR variants originating from the surface of the Arctic Ocean. Their absorption maxima were between 517 and 546 nm at pH 7. One of the isolates turned out to be identical to G...

journal_title：FEBS letters

authors： Jung JY,Choi AR,Lee YK,Lee HK,Jung KH

更新日期：2008-05-28 00:00:00

abstract：:Localisation of mRNA encoding the four AMPA glutamate receptor subunits in the rat adrenal gland was investigated using in situ hybridisation. GluR1 is found in the zona glomerulosa of the cortex, GluR3 in the remaining parts of the cortex, GluR2 in adrenal medullary cells and GluR4 at a very low level in the zona glo...

journal_title：FEBS letters

authors： Kristensen P

更新日期：1993-10-11 00:00:00

abstract：:Vacuolar membrane H(+)-translocating pyrophosphatase (H(+)-PPase) was purified from pumpkin seedlings. Its enzymatic properties including molecular size of constituting polypeptide (75 kDa) were very similar to those of mung bean H(+)-PPase [(1989) J. Biol. Chem. 264, 20068-20073]. The native, functional molecular siz...

journal_title：FEBS letters

authors： Sato MH,Maeshima M,Ohsumi Y,Yoshida M

更新日期：1991-09-23 00:00:00

abstract：:The C-Jun N-terminal Kinase (JNK) inhibitor SP600125 is widely used to inhibit the JNK-mediated Bax activation and cell apoptosis. However, this report demonstrates that SP600125 synergistically enhances the dihydroartemisinin (DHA)-induced human lung adenocarcinoma cell apoptosis by accelerating Bax translocation and...

journal_title：FEBS letters

authors： Lu YY,Chen TS,Wang XP,Qu JL,Chen M

更新日期：2010-09-24 00:00:00

abstract：:Understanding specific cargo distribution in differentiated cells is a major challenge. Trafficking kinesin proteins (TRAKs) are kinesin adaptors. They bind the cargo binding domain of kinesin-1 motor proteins forming a link between the motor and their cargoes. To refine the TRAK1/2 binding sites within the kinesin-1 ...

journal_title：FEBS letters

authors： Randall TS,Moores C,Stephenson FA

更新日期：2013-11-29 00:00:00

abstract：:Colneleic acid (9-[1'(E),3'(Z)-nonadienyloxy]-8(E)-nonenoic acid) is produced from linoleic acid by the sequential action of 9-lipoxygenase and divinyl ether synthase. We demonstrate that a small fraction of the colneleic acid in potato tubers is esterified in phospholipids. This colneleic acid was released by chemica...

journal_title：FEBS letters

authors： Fauconnier ML,Williams TD,Marlier M,Welti R

更新日期：2003-03-13 00:00:00

abstract：:We have used a panel of monoclonal antibodies in a study of the expression of multiple tubulins in Physarum polycephalum. Three anti-beta-tubulin monoclonal antibodies, DM1B, DM3B3 and KMX-1 all reacted with the beta 1-tubulin isotypes expressed in both myxamoebae and plasmodia. However, these antibodies showed a spec...

journal_title：FEBS letters

authors： Birkett CR,Foster KE,Johnson L,Gull K

更新日期：1985-08-05 00:00:00

abstract：:To investigate the biochemical basis of the HIV-1 resistance to AZT we obtained the RT mutant containing four amino acid substitutions by an oligonucleotide-directed mutagenesis technique. Enzymatic properties of the wild type and mutant RTs were compared. 'AZT-resistant' mutations in RT were shown to be associated wi...

journal_title：FEBS letters

authors： Pokholok DK,Gudima SO,Yesipov DS,Dobrynin VN,Rechinsky VO,Kochetkov SN

更新日期：1993-07-05 00:00:00

abstract：:Zymosan, which is composed primarily of alpha-mannan and beta-glucan polymers, is a well recognized activator of macrophages. The type receptor by which unopsonized zymosan induces arachidonic acid release was investigated. It was found that particulate beta-glucan and zymosan stimulated an identical dose-dependent re...

journal_title：FEBS letters

authors： Daum T,Rohrbach MS

更新日期：1992-09-07 00:00:00

abstract：:500 MHz 1H NMR studies of human epidermal growth factor are described. The backbone resonances of the 1-48 derivative of hEGF have been assigned using two-dimensional techniques. Analysis of the type and magnitude of the observed sequential nuclear Overhauser effects and the NH-alpha CH spin-spin coupling constants al...

journal_title：FEBS letters

authors： Carver JA,Cooke RM,Esposito G,Campbell ID,Gregory H,Sheard B

更新日期：1986-09-01 00:00:00

abstract：:Coenzyme A ligases play an important role in metabolism by catalyzing the activation of carboxylic acids. In this study we describe the synthesis of aminoacyl-coenzyme As (CoAs) catalyzed by a CoA ligase from Penicillium chrysogenum. The enzyme accepted medium-chain length fatty acids as the best substrates, but the p...

journal_title：FEBS letters

authors： Koetsier MJ,Jekel PA,Wijma HJ,Bovenberg RA,Janssen DB

更新日期：2011-03-23 00:00:00

abstract：:CXCR4 (fusin) is a chemokine receptor which is involved as a coreceptor in gp120 binding to the cell surface. In this study we provide evidence that binding of gp120 triggers CXCR4 recruitment to glycosphingolipid-enriched microdomains. Scanning confocal microscopy showed a nearly complete localization of CXCR4 within...

journal_title：FEBS letters

authors： Sorice M,Garofalo T,Misasi R,Longo A,Mattei V,Sale P,Dolo V,Gradini R,Pavan A

更新日期：2001-09-28 00:00:00

abstract：:Feeding insects introduce oral secretions (OS) into the wounded tissue of the attacked plant. Various OS-derived molecules must be involved in subsequent processes including the induction of plant defence reactions. Using the planar lipid bilayer membrane technique, isolated OS were analyzed with respect to their memb...

journal_title：FEBS letters

authors： Maischak H,Grigoriev PA,Vogel H,Boland W,Mithöfer A

更新日期：2007-03-06 00:00:00

abstract：:The Escherichia coli signal transduction protein PII, product of the glnB gene, was overproduced and purified. The predicted molecular weight of the protein based on the correct nucleotide sequence is 12,427 and is very close to the value 12,435 obtained by matrix-assisted laser desorption mass spectrometry. Hexagonal...

journal_title：FEBS letters

authors： Vasudevan SG,Gedye C,Dixon NE,Cheah E,Carr PD,Suffolk PM,Jeffrey PD,Ollis DL

更新日期：1994-01-17 00:00:00

abstract：:Lectin-like oxidized low-density lipoprotein receptor (LOX-1) is a scavenger receptor that binds oxidized low-density lipoprotein (OxLDL) and has a role in atherosclerosis development. The N-terminus intracellular region (cytoplasmic domain) of LOX-1 mediates receptor internalization and trafficking, potentially throu...

journal_title：FEBS letters

authors： Bakthavatsalam D,Soung RH,Tweardy DJ,Chiu W,Dixon RA,Woodside DG

更新日期：2014-06-13 00:00:00

abstract：:SigT is an ECF sigma factor in Streptomyces coelicolor. sigT and its putative anti-sigma factor gene rstA are located in one putative operon, and SigT could physically interact with RstA. Deletion of sigT or rstA caused accelerated morphological development and enhanced production of antibiotics, concomitant with over...

journal_title：FEBS letters

authors： Mao XM,Zhou Z,Cheng LY,Hou XP,Guan WJ,Li YQ

更新日期：2009-10-06 00:00:00

abstract：:A drastic inhibition of protein synthesis occurs in HeLa cells treated with human lymphoblastoid interferon and infected with poliovirus. At the time when this inhibition has been established no degradation of 32P-labelled ribosomal RNA can be detected. Isolation of the mRNAs from poliovirus-infected cells plus or min...

journal_title：FEBS letters

authors： Muñoz A,Harvey R,Carrasco L

更新日期：1983-08-22 00:00:00

abstract：:Insolubility in non-ionic detergents such as Triton X-100 is a widely used biochemical criterion for characterization of membrane domains. We report here a novel green fluorescent protein fluorescence-based approach to directly determine detergent insolubility of specific membrane proteins. We have applied this method...

journal_title：FEBS letters

authors： Kalipatnapu S,Chattopadhyay A

更新日期：2004-10-22 00:00:00

abstract：:The tryptophan analog, 5-hydroxytryptophan (5HW), has a significant absorbance between 310-320 nm, which allows it to act as an exclusive fluorescence probe in protein mixtures containing a large number of tryptophan residues. Here for the first time a method is reported for the biosynthetic incorporation of 5HW into ...

journal_title：FEBS letters

authors： Hogue CW,Rasquinha I,Szabo AG,MacManus JP

更新日期：1992-10-05 00:00:00

abstract：:The effort to understand the mechanism of uncoupling by UCP has devolved into two models - the fatty acid protonophore model and the proton buffering model. Evidence for each hypothesis is summarized and evaluated. We also evaluate the obligatory requirement for fatty acids in UCP1-mediated uncoupling and the question...

journal_title：FEBS letters

authors： Garlid KD,Jabůrek M,Jezek P

更新日期：1998-10-30 00:00:00

abstract：:Glycosylphosphatidylinositol (GPI) is synthesized in the endoplasmic reticulum (ER) and added onto proteins to form GPI-anchored proteins. Among the many proteins involved in this process, ACAT-related enzyme-2 required for viability 1 (Arv1) is a candidate, functioning as a flippase that translocates GPI intermediate...

journal_title：FEBS letters

authors： Okai H,Ikema R,Nakamura H,Kato M,Araki M,Mizuno A,Ikeda A,Renbaum P,Segel R,Funato K

更新日期：2020-08-01 00:00:00

abstract：:The substrate specificity of purified PKC-alpha, -beta and -gamma has been investigated. A series of synthetic peptides based upon the sequence surrounding serine-7 in glycogen synthase were generated and used to determine the basic residue requirements of these PKC isotypes. While PKC-alpha and -beta are indistinguis...

journal_title：FEBS letters

authors： Marais RM,Nguyen O,Woodgett JR,Parker PJ

更新日期：1990-12-17 00:00:00

abstract：:The primary structure of nuclear factor-I (CTF/NF-I), a eukaryotic regulatory DNA-binding protein involved in both DNA replication and gene transcription, and the secondary structure predictable from it, are compared here with those of a number of prokaryotic acetylases. Hydropathy and Chou-Fasman analyses reveal that...

journal_title：FEBS letters

authors： Oikarinen J,Mannermaa RM

更新日期：1990-10-29 00:00:00

abstract：:The glycogen-binding (G) subunit of protein phosphatase-1 is phosphorylated in vivo. In rabbits injected with propranolol the serine residue termed site-1 was phosphorylated in 56% of the molecules isolated, and phosphorylation increased to 82% after administration of adrenalin. It is concluded that the G-subunit is a...

journal_title：FEBS letters

authors： MacKintosh C,Campbell DG,Hiraga A,Cohen P

更新日期：1988-07-04 00:00:00

abstract：:The ubiquitous transcription factor Sp1 has been implicated in the mechanism which maintains CpG islands methylation-free. Plasmids containing GC boxes (Sp1 sites) were in vitro methylated at every CpG dinucleotide. After stable introduction into F9 embryonal carcinoma cells, we analysed the methylation of the sequenc...

journal_title：FEBS letters

authors： Silke J,Rother KI,Georgiev O,Schaffner W,Matsuo K

更新日期：1995-08-21 00:00:00