Abstract:
:Vacuolar membrane H(+)-translocating pyrophosphatase (H(+)-PPase) was purified from pumpkin seedlings. Its enzymatic properties including molecular size of constituting polypeptide (75 kDa) were very similar to those of mung bean H(+)-PPase [(1989) J. Biol. Chem. 264, 20068-20073]. The native, functional molecular size of the pumpkin H(+)-PPase was estimated to be 135-139 kDa from gel permeation HPLC of the purified enzyme in the presence of detergent and from radiation inactivation of the enzyme in vacuolar membranes. It is concluded that native, functional pumpkin H(+)-PPase, and also probably H(+)-PPases from other plants, is a dimer of 75 kDa subunits.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sato MH,Maeshima M,Ohsumi Y,Yoshida Mdoi
10.1016/0014-5793(91)81254-6keywords:
subject
Has Abstractpub_date
1991-09-23 00:00:00pages
177-80issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(91)81254-6journal_volume
290pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Carotenoids present in lipids extracted from the cyanobacterium Synechococcus 6716 indicate trans-membrane potential in proteoliposomes reconstituted from these lipids and the ATPase complex isolated from the same organism. A carotenoid absorbance band shift to a longer wavelength is obtained with valinomycin-induced ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80661-x
更新日期:1985-05-06 00:00:00
abstract::Thrombin is a potent activator of human glomerular epithelial cells (HGEC). Here we compare short-term and long-term effects of thrombin and thrombin receptor agonist peptide (TRAP) which selectively activates the functional thrombin receptor. TRAP, as thrombin, increases intracellular free Ca2+ concentration and acts...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81716-d
更新日期:1993-11-15 00:00:00
abstract::In fibrous connective tissues, fibroblasts are organized into syncytia, cellular networks that enable matrix remodeling and that are interconnected by intercellular adherens junctions (AJs). The AJs of fibroblasts are mediated by N-cadherin, a broadly expressed classical cadherin that is critically involved in develop...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2006.12.029
更新日期:2007-01-23 00:00:00
abstract::We present a rat model in which continuous supply of hepatocyte growth factor (HGF) prevents liver injury induced by carbon tetrachloride (CCl4) and E. coli 011:B4 lipopolysaccharide (LPS). Rat fibroblasts genetically modified to secrete rat HGF were implanted in syngenic rat spleen 7 days before administration of the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00744-8
更新日期:1997-07-14 00:00:00
abstract::Modulation of intracellular guanosine 3',5'-bispyrophosphate ((p)ppGpp) level, the effector of the stringent response, is crucial for survival as well as optimal growth of prokaryotes and, thus, for bacterial pathogenesis and dormancy. In Mycobacterium tuberculosis (Mtb), (p)ppGpp synthesis and degradation are carried...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12739
更新日期:2017-08-01 00:00:00
abstract::The trend of the electric field and the value of the electric field flux, sensed by the superoxide substrate in the proximity of the active site, were found to be constant in three highly homologous Cu,Zn superoxide dismutases from ox, pig and sheep, which display large differences in net protein charge and distributi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80681-7
更新日期:1989-06-19 00:00:00
abstract::The peroxisome assembly factor-1 (PAF-1) is reported here to contain the signature subsequence for a ring finger motif in its carboxyl-terminus. This conserved subsequence in PAF-1 may be the key to a gene expression regulatory pathway important in peroxisome biogenesis. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81397-5
更新日期:1992-11-02 00:00:00
abstract::Because A2 adenosine receptor activation stimulates adenylate cyclase and cyclic AMP induces 5'-nucleotidase expression in rat mesangial cells, we examined the effect of adenosine and its analogs on 5'-nucleotidase activity in these cells. A2 adenosine receptors were characterized using [3H]5'-N-ethylcarboxamidoadenos...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80304-d
更新日期:1993-09-27 00:00:00
abstract::Mutations in PTEN-induced putative kinase 1 (PINK1) cause recessive form of Parkinson's disease (PD). PINK1 acts upstream of parkin, regulating mitochondrial integrity and functions. Here, we show that PINK1 in combination with parkin results in the perinuclear mitochondrial aggregation followed by their elimination. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.02.016
更新日期:2010-03-19 00:00:00
abstract::The multiline signal of the S(2) state in Photosystem II was measured both in frozen-solution and single-crystal preparations from the cyanobacterium Thermosynechococcus elongatus. The frozen-solution EPR spectrum shows a gaussian-like line shape without any resolution of Mn hyperfine couplings. This line shape can be...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.05.039
更新日期:2006-06-26 00:00:00
abstract::On the basis of its predicted homology to human telomerase reverse transcriptase (hTERT), a cDNA for Arabidopsis thaliana TERT (AtTERT) has now been isolated from cultured cells. The cDNA contains an open reading frame of 3372 bp, encoding a protein with a predicted size of 131 kDa and isoelectric point of 9.9. The At...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01083-2
更新日期:1999-09-03 00:00:00
abstract::A hybrid protein from Neisseria meningitidis, which contains both a peroxiredoxin and a glutaredoxin domain, has been isolated. The enzyme was active in the reduction of various peroxides and dehydroascorbate in the presence of reduced glutathione. These findings suggest that both the peroxiredoxin and glutaredoxin do...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01156-6
更新日期:2003-11-06 00:00:00
abstract::The octameric ring protein, Translin, demonstrates marked similarities to the family of helicase enzymes regarding its quaternary organization and dimerization of subunits. Here we show that the level of Translin closely parallels the proliferative state in various cell types. Expression is periodic during the cell cy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03095-8
更新日期:2002-08-14 00:00:00
abstract::A large protein complex (approx. 2000 kDa) was found in the cytosol of the hyperthermophilic archaebacterium Staphylothermus marinus. The purified protein was shown to be a homomultimer of 93 kDa subunits, the primary structure of which was determined by nucleotide sequence analysis. The protein belongs to the family ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01304-7
更新日期:1994-12-19 00:00:00
abstract::During the course of large scale purification of the D1 dopamine receptor from rat brain, a protein of approximately 87,000 daltons (p87) was observed to copurify with the D1 receptor through four chromatographic steps. To characterize the nature of this protein, bovine and rat cDNA clones were isolated and sequenced....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80917-6
更新日期:1992-11-09 00:00:00
abstract::Human synovium was analyzed for the possible expression of cartilage oligomeric matrix protein (COMP). Immunostaining with polyclonal antiserum to COMP demonstrated positive staining within the synovial cells and immediately subjacent connective tissue, with less intense staining in the deeper connective tissue. Weste...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00789-8
更新日期:1997-07-21 00:00:00
abstract::Monomeric and trimeric PS I complexes missing the three stromal subunits E,C and D (termed PS I core complexes) were prepared from the thermophilic cyanobacterium Synechococcus sp. by incubation with urea. The subunits E,C and D are sequentially removed. In the monomeric PS I the subunit C is removed with a half life ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80364-1
更新日期:1994-01-31 00:00:00
abstract::Leaflets of Vicia faba with closed stomata or with opening stomata were freeze-dried. Excised guard-cell pairs were assayed individually under suboptimal conditions (pH 7.1 and subsaturating substrate) for phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) using quantitative histochemical procedures. L-Malate, 400 mi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00916-3
更新日期:1994-09-19 00:00:00
abstract::According to the leading theory, the agent responsible for prion diseases would be the conformational isomer PrP(Sc) of a cellular protein PrP(C), the pathogenic form PrP(Sc) multiplying by converting the normal protein into a likeness of itself. The pathogenic isoform could catalyze the conformational transition so t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00310-4
更新日期:1997-04-21 00:00:00
abstract::Dark-adapted isolated spinach chloroplasts and leaves, unlike sub-chloroplast fractions, are capable of emitting ultraweak light spontaneously (50-125 counts/s per cm2). The emission of leaves is due to two processes with activation energies of 97 and 25 kJ/mol while in isolated chloroplasts, it is the result of a sin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81454-v
更新日期:1990-11-26 00:00:00
abstract::The fluorescent calcium indicator 'quin2' was used to demonstrate changes in cytoplasmic calcium concentrations in bovine anterior pituitary cells. The basal calcium concentration was 0.21 +/- 0.02 microM (mean of 4 cell preparations). Thyroliberin (TRH) (10(-10) - 10(-6) M) rapidly and at the higher concentrations tr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80420-7
更新日期:1983-08-08 00:00:00
abstract::HslVU is an ATP-dependent protease consisting of two multimeric components, the HslU ATPase and the HslV peptidase. To gain an insight into the role of HslVU in regulation of cell division, the reconstituted enzyme was incubated with SulA, an inhibitor of cell division in Escherichia coli, or its fusion protein with m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00935-7
更新日期:1999-07-30 00:00:00
abstract::The A-rich bulge in paired region P5a of the Tetrahymena intron is a structural feature that is conserved in the sub-group Ib self-splicing introns. We have constructed a series of substitution and deletion mutations in this region of the intron. Kinetic analysis has shown that some of the mutants have a reduced maxim...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80230-z
更新日期:1991-03-11 00:00:00
abstract::Lysosomal/phagosomal membranes of mammalian cells are coated by highly conserved glycoproteins (lamps) that are thought to protect the membranes from degradation. Interestingly, we identified an amino acid sequence in human lamp-1 characteristic of a cleavage site for the Neisseria gonorrhoeae IgA1 protease. Furthermo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00163-4
更新日期:1997-03-17 00:00:00
abstract::Integral cGMP-dependent currents as well as activity of single cGMP-activated channels in plasma membrane of rod outer segment (ROS) were recorded using the patch-clamp method. The dependence of integral currents on cGMP concentration is shown to be bell-shaped. Decrease in cGMP-dependent conductance at high cGMP conc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80089-2
更新日期:1990-01-15 00:00:00
abstract::We have shown that OSCP, a subunit of yeast mitochondrial ATP synthase, can be incorporated into the intact enzyme as a fusion protein representing OSCP fused at its C-terminus to the green fluorescent protein (GFP) of Aequorea victoria. The relevant fusion OSCP-GFP-h6 additionally contains a hexahistidine tag at the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00670-4
更新日期:1997-07-07 00:00:00
abstract::A cytochrome c (cyt. c) was solubilized with Triton-X-100 and co-purified with cytochrome c oxidase from membranes of chemotrophically grown cells of Rhodopseudomonas capsulata. Cyt. c and cytochrome oxidase were separated on Sephadex G-50 columns. Antibodies against cytochrome c2 from the same bacterium did not cross...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80390-1
更新日期:1983-02-21 00:00:00
abstract::Activation-induced cytidine deaminase (AID), a member of the APOBEC family that induces antibody diversification, has been shown to inhibit the replication of hepatitis B virus, Kaposi's sarcoma-associated herpesvirus, and retro-transposons. However, whether AID can inhibit human immunodeficiency virus 1 (HIV-1) repli...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12954
更新日期:2018-01-01 00:00:00
abstract::We prepared an alpha-bungarotoxin-specific monoclonal antibody that neutralizes the biological activity of the toxin in vivo. The antigenic determinant combining specifically with this antibody was determined on the basis of cross-reaction experiments using three other long neurotoxins and peptide fragments of alpha-b...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81018-x
更新日期:1989-08-28 00:00:00
abstract::Thrombin is a multifunctional enzyme involved in coagulation, cell modulation and inflammation. We recently reported a novel membrane-associated prothrombin activator, abbreviated as MAPA, found in cultured fibroblasts and glial cell lines. In this study, we examined the physiological role of this enzyme. MAPA-like ac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00852-1
更新日期:1997-08-04 00:00:00