Lessened malate inhibition of guard-cell phosphoenolpyruvate carboxylase velocity during stomatal opening.

Abstract:

:Leaflets of Vicia faba with closed stomata or with opening stomata were freeze-dried. Excised guard-cell pairs were assayed individually under suboptimal conditions (pH 7.1 and subsaturating substrate) for phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) using quantitative histochemical procedures. L-Malate, 400 microM, significantly inhibited guard-cell PEPC activity of closed stomata but not that of opening stomata. We postulate that the lessened sensitivity of guard-cell PEPC activity to malate inhibition is an important regulatory feature of stomatal opening, which is associated with malate accumulation.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Zhang SQ,Outlaw WH Jr,Chollet R

doi

10.1016/0014-5793(94)00916-3

subject

Has Abstract

pub_date

1994-09-19 00:00:00

pages

45-8

issue

1

eissn

0014-5793

issn

1873-3468

pii

0014-5793(94)00916-3

journal_volume

352

pub_type

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