Abstract:
:Treatment of 125I-labelled high-density lipoprotein ([125I]HDL3) with monospecific polyclonal antibodies against apolipoproteins A-I and A-II resulted in a dose-dependent inhibition of the [125I]HDL3 binding to isolated human small intestine epithelial cells by 25% and 50%, respectively. Both antibodies also inhibited intracellular degradation of [125I]HDL3 by 80%. Treatment of enterocytes with polyclonal antibody against apolipoprotein A-I binding protein, a putative HDL receptor, inhibited both binding and degradation of [125I]HDL3 by these cells by 50%. Antibodies to apolipoprotein A-I, A-II and apo A-I-binding protein also inhibited [125I]HDL3 binding to cholesterol-loaded cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sviridov DD,Ehnholm C,Tenkanen H,Pavlov MYu,Safonova IG,Repin VSdoi
10.1016/0014-5793(92)80519-mkeywords:
subject
Has Abstractpub_date
1992-06-01 00:00:00pages
202-4issue
2-3eissn
0014-5793issn
1873-3468journal_volume
303pub_type
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