Abstract:
:T helper 1 (Th1) cell fate is induced by overlapping signaling pathways, whose kinetic principles and regulatory motifs are largely unknown. We identified a simple positive feedback loop in the STAT4 signaling pathway, whereby activation by IL-12 leads to the increased expression in IL-12 receptor. A computational analysis shows that this feedback loop synergizes with the one mediated by the IFN-gamma secreted by differentiating cells, when the induction of Th1 cell fate is weak. Positive feedback loops are often utilized to enhance phenotypic differentiation. This effect was confirmed by experiments showing that stochastic fluctuations in the expression of IL-12 receptor gene were amplified, leading to two discrete levels of expression in a cell population.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Becskei A,Grusby MJdoi
10.1016/j.febslet.2007.10.007subject
Has Abstractpub_date
2007-11-13 00:00:00pages
5199-206issue
27eissn
0014-5793issn
1873-3468pii
S0014-5793(07)01064-2journal_volume
581pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Synaptotagmin I, the calcium sensor for neurotransmission, is palmitoylated. We have identified the palmitoylation sites as five cysteine residues located between the transmembrane and cytoplasmic regions. In contrast to wild-type synaptotagmin, the non-acylated mutant is not converted to the endoglycosidase-H-resista...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00449-6
更新日期:2003-06-05 00:00:00
abstract::To investigate the role of lipopolysaccharide (LPS) in hepatocyte activation, we examined the expression of Toll-like receptor 4 (TLR4), the putative receptor for LPS in human hepatocytes. TLR4 mRNA and protein expression was confirmed in human hepatocytes. Stimulation of human hepatocytes with LPS results in rapid de...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.05.072
更新日期:2004-07-02 00:00:00
abstract::The mongoose AChR alpha-subunit has been cloned and shown to be highly homologous to other AChR alpha-subunits, with only six differences in amino acid residues at positions that are conserved in animal species that bind alpha-bungarotoxin (alpha-BTX). Four of these six substitutions cluster in the ligand binding site...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00341-x
更新日期:1998-04-17 00:00:00
abstract::Acceptor stem variants of tRNA(fMet) (Escherichia coli) have been characterized by nuclear magnetic resonance. The wild type contains a C1-A72 mismatch pair which is crucial for its biological function. For comparison, the mismatch was replaced by regular pairs U1-A72 and C1-G72. Further variants contain an altered di...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01459-9
更新日期:2000-04-28 00:00:00
abstract::The present study shows a direct impairing action of a cytotoxin-producing Helicobacter pylori strain on the Na+,K(+)-ATPase (evaluated as K(+)-dependent phosphatase activity) of human gastric epithelial cells in culture. The toxin itself is likely involved in this action which may also account for the cell edema foun...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81703-3
更新日期:1993-11-15 00:00:00
abstract::N-Biotinyl phosphatidylethanolamine spin labelled at the C-14 position of the sn-2 chain has been incorporated at a level of 1 mol% in bilayers of dimyristoyl phosphatidylcholine, and the effects on the chain mobility of binding avidin to the biotin lipid headgroup have been studied by electron spin resonance spectros...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81531-4
更新日期:1993-06-07 00:00:00
abstract::The NAD-dependent glycerol-3-phosphate dehydrogenase (glycerol-3-phosphate:NAD+ oxidoreductase; EC 1.1.1.8; G3P DHG) was purified 178-fold to homogeneity from Saccharomyces cerevisiae strain H44-3D by affinity- and ion-exchange chromatography. SDS-PAGE indicated that the enzyme had a molecular mass of approximately 42...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81259-o
更新日期:1992-08-17 00:00:00
abstract::The primary structure of nuclear factor-I (CTF/NF-I), a eukaryotic regulatory DNA-binding protein involved in both DNA replication and gene transcription, and the secondary structure predictable from it, are compared here with those of a number of prokaryotic acetylases. Hydropathy and Chou-Fasman analyses reveal that...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81039-q
更新日期:1990-10-29 00:00:00
abstract::Microinjection of human liver mRNA from a patient homozygous for alpha 1-antitrypsin deficiency (PiZZ) into Xenopus oocytes led to a 2--10-fold increase in lysosomal activity. Stimulation of lysosomal activity was not observed when mRNA from a normal human liver (alpha 1-antitrypsin PiMM), or water was injected into t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80798-5
更新日期:1985-04-22 00:00:00
abstract::The possibility that the mutant mouse wasted (wst/wst) may serve as an animal model for studies of severe combined immunodeficiency disease (SCID) and the role of adenosine deaminase (ADA, EC 3.5.4.4) in adenosine metabolism were investigated. The specific activity of ADA in wst/wst compared with control mice was sign...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)81063-8
更新日期:1986-11-24 00:00:00
abstract::Gp130 is the common signal transducing receptor subunit of interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor and cardiotrophin-1. IL-6 and IL-11 induce gp130 homodimerization whereas the others lead to the formation of heterodimers with LIFR or OSMR. Binding e...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01205-9
更新日期:2000-02-25 00:00:00
abstract::The reduction of methyl-coenzyme M (CH3SCoM) to methane in methanogenic bacteria is dependent on component B (N-7-mercaptoheptanoyl-O-phospho-L-threonine, HSHTP). We report here that S-methyl-component B (N-7-(methylthio)heptanoyl-O-phospho-L-threonine, CH3SHTP) can substitute for neither CH3SCoM nor HSHTP in the meth...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80846-3
更新日期:1987-08-17 00:00:00
abstract::Osteoblastic differentiation is regulated by various factors, including hormones and transcription factors. Runt-related transcription factor 2 (Runx2) is an essential player in osteoblastogenesis and transactivates its molecular target by creating a protein complex with its hetero-dimeric partner core binding factor ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.09.024
更新日期:2015-10-24 00:00:00
abstract::Hyperthyroid mitochondria show an increased Km and Vmax in the high affinity phase of cytochrome oxidase kinetics. During inhibitor titrations, cytochrome c shows a different redox behaviour in hyperthyroid with respect to protonophore-treated euthyroid mitochondria. The observations are discussed in terms of a differ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01211-v
更新日期:1995-11-20 00:00:00
abstract::Physiological amyloid aggregation occurs within the nuclei of stress-treated cells. These structures, termed Amyloid bodies (A-bodies), assemble through the rapid accumulation of proteins into dense membrane-less organelles, which possess the same biophysical properties as plaques observed in many amyloid-based diseas...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13597
更新日期:2019-11-01 00:00:00
abstract::We present an open-access web platform isomiRex, to identify isomiRs and on the fly graphical visualization of the differentially expressed miRNAs in control as well as treated library. The open-access web-platform is not restricted only to NGS sequence dataset from animals and potentially analyzes a wider dataset for...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.06.047
更新日期:2013-08-19 00:00:00
abstract::Disruption of the gene pgil of Saccharomyces cerevisiae, which codes for phosphoglucose isomerase, results in a dramatic increase in the amount of intracellular glycogen in early exponential cultures. The level of glucose 6-phosphate was much higher in mutant than in wild-type cells. Phosphorylase a activity and the s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81325-g
更新日期:1992-09-28 00:00:00
abstract::Hematopoietic progenitors have been shown to retain plasticity and switch lineages by appropriate stimuli. However, mature blood cells hardly showed such differentiation plasticity. In this paper, we tried to reprogram mature B cells into erythroid lineage by expressing various hematopoietic transcription factors. Amo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.08.019
更新日期:2012-10-19 00:00:00
abstract::The role of protein kinase C (PKC) in cellular regulation of L-type Ca2+ channels was investigated in human umbilical vein smooth muscle. Activation of PKC, by low concentrations (< 30 nM) of 12-O-tetradecanoyl-phorbol-13-acetate (TPA) caused inhibition of Ca2+ channels, while higher concentrations of TPA (> 100 nM) e...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80458-3
更新日期:1994-03-21 00:00:00
abstract::We have reported previously on the ability of Lactobacillus reuteri to produce a compound with vitamin B(12) activity. Here we report on the chemical characterisation of this corrinoid-like molecule. High performance liquid chromatography coupled to an ultraviolet diode array detector, mass spectrometry and nuclear ma...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.09.012
更新日期:2007-10-16 00:00:00
abstract::Highly selective affinity labeling of the primer site of E. coli DNA polymerase I was performed with the 5'-reactive derivatives of oligothymidylate in the presence of poly(dA) template. Subtilysine cleavage proved that the site of affinity modification belonged to the 'Klenow' part of DNA polymerase I. If taken separ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80478-2
更新日期:1990-10-15 00:00:00
abstract::Trypanosomes are unicellular parasites and like all decent parasites, they try to obtain from the host as much material as possible, including lipids. However, the needs of a parasite are not always the same as those of the host, and therefore, mostly, some biosynthetic work still has to be done by the parasite itself...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2006.07.056
更新日期:2006-10-09 00:00:00
abstract::Mitochondria in primary living hepatocytes were visualized in cells transfected with a chimeric plasmid encoding for the green fluorescent protein (GFP) of Aequorea victoria engineered to be specifically targeted to mitochondria, as described recently (Rizutto et al. (1995) Curr. Biol. 5, 635-642). The identification ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00182-2
更新日期:1996-03-18 00:00:00
abstract::Using PCR-based techniques, we have isolated and sequenced the full-length cDNAs that encode the human neuronal nAChR subunits alpha3,4,5,7 and beta2,3,4 in the neuroblastoma cell lines SH-SY5Y and/or IMR-32. The predicted nAChR beta3- and beta4-subunit proteins contain 458 and 498 amino acids, respectively. Except fo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01383-x
更新日期:1997-01-06 00:00:00
abstract::The present study discusses the efficacy of Aloe emodin-8-O-glycoside (AEG), a plant derived anthroquinone, on alleviating insulin resistance and augmenting glycogen synthesis in L6 myotubes and 3T3L1 adipocytes. Dose-dependent increase in glucose uptake activity (GUA) was observed in both cell lines. Immunoblot analy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.06.004
更新日期:2010-07-16 00:00:00
abstract::Proteins with a CyaB, thiamine triphosphatase domain (CYTH domain) may play a central role at the interface between nucleotide and polyphosphate metabolism. One of the plant CYTH domain-containing proteins from Brachypodium distachyon, BdTTM3, is annotated in NCBI databases as an 'adenylyl cyclase (AC)' or a 'triphosp...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13701
更新日期:2020-03-01 00:00:00
abstract::We have identified in rat vascular smooth muscle cells (SMCs) the simultaneous expression of two TGF-beta type I receptor (ALK-5) cDNAs, occurring as a consequence of alternate usage of AG splice acceptor motifs separated by 12 nucleotides located at an intron-exon junction. When translated the resultant full length p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01132-7
更新日期:2000-02-04 00:00:00
abstract::A reversible two-step (native state<-->denatured state) folding mechanism based on equilibrium and stopped flow experiments is proposed for urea denaturation of the lipoyl-bearing domain (hbLBD) of human mitochondrial branched chain alpha-ketoacid dehydrogenase (BCKD) complex. The results from this circular dichroism ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03444-0
更新日期:2002-10-23 00:00:00
abstract::Antithrombin Glasgow is a hereditary abnormal antithrombin that has lost thrombin inhibitory activity. It was isolated from the plasma of a 41-year-old male with a history of thrombotic events. Antithrombin Glasgow was purified from plasma using heparin-Sepharose chromatography at pH 7.4 eluting with increasing concen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80841-x
更新日期:1988-04-25 00:00:00
abstract::We examined whether a proposed spatial proximity between Asp114(2.50) and Asn332(7.49) affected the functional properties of the mu opioid receptor. The D114(2.50)N mutant had reduced binding affinities for morphine, DAMGO and CTAP, but not for naloxone and [3H]diprenorphine; this mutation also abolished agonist-induc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00316-6
更新日期:1999-03-26 00:00:00