Abstract:
:We have identified in rat vascular smooth muscle cells (SMCs) the simultaneous expression of two TGF-beta type I receptor (ALK-5) cDNAs, occurring as a consequence of alternate usage of AG splice acceptor motifs separated by 12 nucleotides located at an intron-exon junction. When translated the resultant full length proteins differ from each other only by the in-frame presence or absence of Gly-Pro-Phe-Ser residues adjacent to their transmembrane domain. Stable expression of these alternate ALK-5 isoforms in ALK-5-deficient cells demonstrated that both were competent in signaling TGF-beta-induced growth inhibition and gene transcription, but with an apparently distinct potency. Our data suggest that alternate splicing within the ALK-5 gene is an important mechanism whereby SMCs may regulate their response to TGF-beta.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Agrotis A,Condron M,Bobik Adoi
10.1016/s0014-5793(00)01132-7keywords:
subject
Has Abstractpub_date
2000-02-04 00:00:00pages
128-32issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(00)01132-7journal_volume
467pub_type
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