Abstract:
:C26, the C-terminal 26 residue peptide of serpin A1, significantly increased cell proliferation in cultures of hepatoma cells, but not in porcine kidney epithelial cells, human skin fibroblasts or keratinocytes. The mitogenic activity of C26 was preferentially inhibited with a protein kinase C (PKC) inhibitor, an antibody against CD47 and CD47 short interfering RNA. The mutant C26-K19R,N22M, imitating a thrombospondin-like cell adhesion motif, increased the mitogenic activity in both Hep G2 cells and MCF-7 breast cancer cells. Phosphorylation of C26 at T24 (a putative PKC phosphorylation site) resulted in a 1.9-2.5 increase in mitogenic activity over C26 in MCF-7 cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Congote LF,Temmel Ndoi
10.1016/j.febslet.2004.09.035keywords:
subject
Has Abstractpub_date
2004-10-22 00:00:00pages
343-7issue
3eissn
0014-5793issn
1873-3468pii
S0014579304011500journal_volume
576pub_type
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