Abstract:
:The cDNA coding for mature human alpha 1-proteinase inhibitor (alpha 1-PI) has been inserted into a variety of yeast expression vectors. Yeast cells transformed with these plasmids were then assayed for the production of mature, unglycosylated alpha 1-PI. The production level is optimal when the recombinant plasmid carries the TDH promoter, the complete 2mu and the leu2D selection marker. Biologically active recombinant alpha 1-PI can be purified either analytically, by affinity chromatography using a monoclonal antibody, or on a large scale, by a procedure involving precipitation of high-Mr yeast material with polyethylene glycol 3300 followed by successive chromatography on DEAE-agarose, Zn-chelate agarose, kappa-chain agarose, heparin-agarose and aminohexyl-agarose.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Hoylaerts M,Weyens A,Bollen A,Harford N,Cabezón Tdoi
10.1016/0014-5793(86)81391-6subject
Has Abstractpub_date
1986-08-11 00:00:00pages
83-7issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(86)81391-6journal_volume
204pub_type
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