The Saccharomyces cerevisiae OXA1 gene is required for the correct assembly of cytochrome c oxidase and oligomycin-sensitive ATP synthase.


:The nuclear gene OXA1 was first isolated in Saccharomyces cerevisiae and found to be required at a post-translational step in cytochrome c oxidase biogenesis, probably at the level of assembly. Mutations in OXA1 lead to a complete respiratory deficiency. The protein Oxa1p is conserved through evolution and a human homolog has been isolated by functional complementation of a yeast oxa1- mutant. In order to further our understanding of the role of Oxa1p, we have constructed two yeast strains in which the OXA1 open reading frame was almost totally deleted. Cytochrome spectra and enzymatic activity measurements show the absence of heme aa3 and of a cytochrome c oxido-reductase activity and dramatic decrease of the oligomycin sensitive ATPase activity. Analysis of the respiratory complexes in non-denaturing gels reveals that Oxa1p is necessary for the correct assembly of the cytochrome c oxidase and the ATP synthase complex.




FEBS letters


Altamura N,Capitanio N,Bonnefoy N,Papa S,Dujardin G




Has Abstract


1996-03-11 00:00:00














  • Evidence for release of free glucose from muscle during amylin-induced glycogenolysis in rats.

    abstract::Amylin, a 37 amino acid product of pancreatic beta-cells, inhibits insulin-stimulated radioglucose incorporation into muscle glycogen. In the current study, we exercised rats and then prelabelled the glycogen pool by infusing [3-3H]glucose along with glucose and insulin. Subsequent amylin administration increased the ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Young AA,Carlo P,Smith P,Wolfe-Lopez D,Pittner R,Wang MW,Rink T

    更新日期:1993-11-22 00:00:00

  • Structural variability of the ubiquitin specific protease DUSP-UBL double domains.

    abstract::USP4, 11 and 15 are three closely related paralogues of the ubiquitin specific protease (USP) family of deubiquitinating enzymes. The DUSP domain and the UBL domain in these proteins are juxtaposed which may provide a functional unit conferring specificity. We determined the structures of the USP15 DUSP-UBL double dom...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Elliott PR,Liu H,Pastok MW,Grossmann GJ,Rigden DJ,Clague MJ,Urbé S,Barsukov IL

    更新日期:2011-11-04 00:00:00

  • Seminalplasmin and caltrin are the same protein.

    abstract::The sequence of seminalplasmin, a basic antimicrobial and transcription-inhibitory protein from bovine seminal plasma, has been determined using an automated sequenator. This sequence is slightly different from that reported earlier by Theil and Scheit [(1983) EMBO J. 2, 1159-1163] and identical with that of caltrin, ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sitaram N,Kumari VK,Bhargava PM

    更新日期:1986-06-09 00:00:00

  • Tumor suppressor RBM5 directly interacts with the DExD/H-box protein DHX15 and stimulates its helicase activity.

    abstract::RNA binding motif protein 5 (RBM5) is a candidate tumor suppressor gene. Recent studies showed that RBM5 functions as an alternative splicing regulator of apoptosis-related genes. Here, we identify DHX15 and PRP19, two spliceosome components, as novel RBM5-interacting partners. We then show that the G-patch domain of ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Niu Z,Jin W,Zhang L,Li X

    更新日期:2012-04-05 00:00:00

  • FixL-like sensor FlbS of Brucella abortus binds haem and is necessary for survival within eukaryotic cells.

    abstract::Replication of Brucella inside eukaryotic cells is essential for pathogenesis, and successful infection requires rapid adaptation to the intracellular milieu. Close relatives of Brucella use the two-component system FixLJ to survive inside the host. We aimed to identify a homologous sensor in Brucella abortus. A predi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Roset MS,Almirón MA

    更新日期:2013-09-17 00:00:00

  • Nuclear phosphoproteins HMGA and their relationship with chromatin structure and cancer.

    abstract::The structural characteristics of the three nuclear phosphoproteins of the high mobility group A family are outlined and related to their participation in chromatin structure alteration in many biological processes such as gene expression, neoplastic transformation, differentiation, and apoptosis. The elevated express...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Sgarra R,Rustighi A,Tessari MA,Di Bernardo J,Altamura S,Fusco A,Manfioletti G,Giancotti V

    更新日期:2004-09-10 00:00:00

  • Parkin-mediated selective mitochondrial autophagy, mitophagy: Parkin purges damaged organelles from the vital mitochondrial network.

    abstract::Cellular homeostasis is linked tightly to mitochondrial functions. Some damage to mitochondrial proteins and nucleic acids can lead to the depolarization of the inner mitochondrial membrane, thereby sensitizing impaired mitochondria for selective elimination by autophagy. Mitochondrial dysfunction is one of the key as...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Tanaka A

    更新日期:2010-04-02 00:00:00

  • The 5'-untranslated region of the human muscle acylphosphatase mRNA has an inhibitory effect on protein expression.

    abstract::The cDNA of the human muscle type acylphosphatase was isolated and characterized. The mRNA presents a very long 5'-untranslated region, covering the first half of the molecule: 175 bases of this part were cloned and prediction of the possible secondary structure showed that a very stable stem-loop structure could be f...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fiaschi T,Marzocchini R,Raugei G,Veggi D,Chiarugi P,Ramponi G

    更新日期:1997-11-03 00:00:00

  • Pharmacological targeting of α-synuclein and TPPP/p25 in Parkinson's disease: challenges and opportunities in a Nutshell.

    abstract::With the aging of population, neurological disorders, and especially disorders involving defects in protein conformation (also known as proteopathies) pose a serious socio-economic problem. So far there is no effective treatment for most proteopathies, including Parkinson's disease (PD). The mechanism underlying PD pa...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Oláh J,Ovádi J

    更新日期:2019-07-01 00:00:00

  • Expanded amino acid sequence of the PhaC box in the active center of polyhydroxyalkanoate synthases.

    abstract::Polyhydroxyalkanoate (PHA) synthases catalyze the polymerization reaction of the acyl moiety of hydroxyacyl-coenzyme A into polyester. The catalytic subunit PhaC of PHA synthase has the PhaC box sequence at the active site that is typically described as G-X-C-X-G-G (X is an arbitrary amino acid), and cysteine is an ac...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nambu Y,Ishii-Hyakutake M,Harada K,Mizuno S,Tsuge T

    更新日期:2020-02-01 00:00:00

  • Photosynthesis modulates the sign of phototaxis of wild-type Chlamydomonas reinhardtii. Effects of red background illumination and 3-(3',4'-dichlorophenyl)-1,1-dimethylurea.

    abstract::We have found that the sign of phototaxis, i.e. the direction of phototactic migration either towards or away from the light source, of wild-type Chlamydomonas reinhardtii depends on its photosynthetic activity. This accounts for the frequently reported transient positive phototaxis that precedes a negative phototaxis...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Takahashi T,Watanabe M

    更新日期:1993-12-28 00:00:00

  • Six myosin heavy chain isoforms are expressed during chick breast muscle development.

    abstract::Two major embryonic myosin heavy chains are expressed in embryonic chick breast muscle until the first week after hatching. Of these, one is already detected in the 8-day-old embryo. The other appears on day 12. Two putative slow embryonic isoforms represent minor components transiently expressed between days 8 and 12...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hofmann S,Düsterhöft S,Pette D

    更新日期:1988-10-10 00:00:00

  • Developmental regulation of expression of the alpha 1 and alpha 2 subunits mRNAs of the voltage-dependent calcium channel in a differentiating myogenic cell line.

    abstract::The voltage-dependent calcium channel (VDCC) in skeletal muscle probably plays a key role in transducing membrane charge movement to the calcium release channel. We report here that the expression of VDCC alpha 1 and alpha 2 mRNAs is developmentally regulated in differentiating C2C12 myogenic cells. The alpha 1 mRNA i...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Varadi G,Orlowski J,Schwartz A

    更新日期:1989-07-03 00:00:00

  • Bacterial beta-galactosidase and human dystrophin genes are expressed in mouse skeletal muscle fibers after ballistic transfection.

    abstract::Ballistic transfection, based on cell and tissue bombardment by the tungsten and gold microparticles covered with the gene DNA, was used for the delivery of a bacterial beta-galactosidase and a full-length cDNA copy of the human dystrophin genes into mouse skeletal muscles. CMV-lacZ, SV40-lacZ, LTR-lacZneo and full-le...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zelenin AV,Kolesnikov VA,Tarasenko OA,Shafei RA,Zelenina IA,Mikhailov VV,Semenova ML,Kovalenko DV,Artemyeva OV,Ivaschenko TE,Evgrafov OV,Dickson G,Baranovand VS

    更新日期:1997-09-08 00:00:00

  • The role of p38 mitogen-activated protein kinase in IL-6 and IL-8 production from the TNF-alpha- or IL-1beta-stimulated rheumatoid synovial fibroblasts.

    abstract::We examined the role of p38 mitogen-activated protein (MAP) kinase in the tumor necrosis factor alpha (TNF-alpha)- or interleukin-1beta (IL-1beta)-induced production of interleukin-6 (IL-6) and interleukin-8 (IL-8) in fresh rheumatoid synovial fibroblast (RSF) cultures concomitantly with the induction of p38 MAP kinas...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Suzuki M,Tetsuka T,Yoshida S,Watanabe N,Kobayashi M,Matsui N,Okamoto T

    更新日期:2000-01-07 00:00:00

  • ATP potentiates the formation of AChR aggregate in the co-culture of NG108-15 cells with C2C12 myotubes.

    abstract::The role of adenosine 5'-triphosphate (ATP) and P2Y(1) nucleotide receptor in potentiating agrin-induced acetylcholine receptor (AChR) aggregation is being demonstrated in a co-culture system of NG108-15 cell, a mouse neuroblastoma X rat glioma hybrid cell line that resembles spinal motor neuron, with C2C12 myotube. I...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ling KK,Siow NL,Choi RC,Tsim KW

    更新日期:2005-04-25 00:00:00

  • Challenges in the analysis of long noncoding RNA functionality.

    abstract::Long noncoding RNA (lncRNA) are emerging as important regulators of diverse biological functions. Although mechanistic models are starting to emerge, it is also clear that the lncRNA field needs appropriate model systems in order to better elucidate the functions of lncRNA and their roles in both physiological and pat...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Leone S,Santoro R

    更新日期:2016-08-01 00:00:00

  • Analogs of human epidermal growth factor which partially inhibit the growth factor-dependent protein-tyrosine kinase activity of the epidermal growth factor receptor.

    abstract::Three site-directed mutants of human epidermal growth factor, Leu-26----Gly, Leu-47----Ala, and Ile-23----Thr, were examined for their ability to stimulate the protein-tyrosine kinase activity of the epidermal growth factor receptor. The receptor binding affinities of the mutant growth factors were 20- to 50-fold lowe...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Matsunami RK,Campion SR,Niyogi SK,Stevens A

    更新日期:1990-05-07 00:00:00

  • Insulin-like growth factor I supports differentiation of cultured osteoblast-like cells.

    abstract::Rat calvaria cells grown in culture for one week had properties of osteoblasts: a high content in alkaline phosphatase and a marked cyclic AMP response to parathyroid hormone (PTH). In short-term experiments, insulin-like growth factor I (IGF I) stimulated the incorporation of [14C] glucose into glycogen. When IGF I w...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schmid C,Steiner T,Froesch ER

    更新日期:1984-07-23 00:00:00

  • Calmodulin binding to human spectrin.

    abstract::Calmodulin is shown to interact with human spectrin dimer. The binding was highly calcium-dependent and observed in two different kinds of experiments. Firstly, affinity chromatography of calmodulin on a Sepharose 4B column with immobilized spectrin, and secondly, partition in aqueous two-phase polymer systems. In the...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Berglund A,Backman L,Shanbhag VP

    更新日期:1984-06-25 00:00:00

  • Phosphorylation sites for ribosomal S6 protein kinases in mouse 3T3 fibroblasts stimulated with platelet-derived growth factor.

    abstract::Platelet release products and purified platelet-derived growth factor stimulated the phosphorylation of ribosomal protein S6 in cultured mouse Balb/c 3T3 fibroblasts. The post-nuclear fraction of the stimulated cells was enriched in S6 kinase activity specific for sites resembling those phosphorylated within intact ce...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wettenhall RE,Chesterman CN,Walker T,Morgan FJ

    更新日期:1983-10-03 00:00:00

  • A comparative picosecond-resolved fluorescence study of tryptophan residues in iron-sulfur proteins.

    abstract::The fluorescence intensity and anisotropy decays of the intrinsic tryptophan emission from six Fe/S proteins (ranging from the very simplest ones to enzyme complexes containing one, two or more Trp residues) were measured. All proteins were examined in the reduced and the oxidized state. In either redox state each pro...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dorovska-Taran V,van Hoek A,Link TA,Visser AJ,Hagen WR

    更新日期:1994-07-18 00:00:00

  • A high yield method for the preparative synthesis of coenzyme A by combination of chemical and enzymic reactions.

    abstract::Dried cells of Brevibacterium ammoniagenes are a good enzyme source for the preparative synthesis of CoA from pantothenic acid, L-cysteine and ATP. A problem with this synthesis is that the CoA synthesis is repressed by negative feedback inhibition by CoA to pantothenate kinase, the first step enzyme for the biosynthe...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Shimizu S,Komaki R,Tani Y,Yamada H

    更新日期:1983-01-24 00:00:00

  • Modulation of phosphoinositide 3-kinase activation by cholesterol level suggests a novel positive role for lipid rafts in lysophosphatidic acid signalling.

    abstract::Methyl-beta-cyclodextrin (MbetaCD) was used to explore a role for cholesterol-enriched plasma membrane microdomains in coupling lysophosphatidic acid (LPA) stimulation to phosphoinositide 3-kinase (PI3K) activation. Cholesterol depletion strongly inhibited the production of phosphatidylinositol 3,4-bisphosphate and ph...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Peres C,Yart A,Perret B,Salles JP,Raynal P

    更新日期:2003-01-16 00:00:00

  • Deregulation of cell signaling in cancer.

    abstract::Oncogenic mutations disrupt the regulatory circuits that govern cell function, enabling tumor cells to undergo de-regulated mitogenesis, to resist to pro-apoptotic insults, and to invade through tissue boundaries. Cancer cell biology has played a crucial role in elucidating the signaling mechanisms by which oncogenic ...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Giancotti FG

    更新日期:2014-08-19 00:00:00

  • Inhibition of angiotensin converting enzyme (ACE) activity by flavan-3-ols and procyanidins.

    abstract::It was determined that flavan-3-ols and procyanidins have an inhibitory effect on angiotensin I converting enzyme (ACE) activity, and the effect was dependent on the number of epicatechin units forming the procyanidin. The inhibition by flavan-3-ols and procyanidins was competitive with the two substrates assayed: N-h...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Actis-Goretta L,Ottaviani JI,Keen CL,Fraga CG

    更新日期:2003-12-18 00:00:00

  • A naturally occurring bacterial Tat signal peptide lacking one of the 'invariant' arginine residues of the consensus targeting motif.

    abstract::Currently described substrates of the bacterial Tat protein transport system are directed for export by signal peptides containing a pair of invariant arginine residues. The signal peptide of the TtrB subunit of Salmonella enterica tetrathionate reductase contains a single arginine residue but is nevertheless able to ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hinsley AP,Stanley NR,Palmer T,Berks BC

    更新日期:2001-05-18 00:00:00

  • Protein kinase C during differentiation of human promyelocytic leukemia cell line, HL-60.

    abstract::Protein kinase C (PKC) from human promyelocytic leukemia HL-60 cells can be resolved into three fractions (peak, a, b and c) by hydroxyapatite column chromatography. Peak a and c enzymes are indistinguishable from the brain type II PKC having beta (beta I and beta II)-sequence and type III having alpha-sequence, respe...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hashimoto K,Kishimoto A,Aihara H,Yasuda I,Mikawa K,Nishizuka Y

    更新日期:1990-04-09 00:00:00

  • Thermodynamic and kinetic control of ATP synthesis in yeast mitochondria: role of delta pH.

    abstract::ATP synthesis rate, measured as the variation in external Pi concentration, varied as a linear function of either delta mu H+ or delta Gpin, in such a manner that the delta Gpin/delta mu H+ ratio increased while VATP increased. We also observed a linear dependence of the flux control coefficient of the Pi carrier on...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Beauvoit B,Rigoulet M,Guerin B

    更新日期:1989-02-27 00:00:00

  • Expression of a Cu,Zn superoxide dismutase typical of familial amyotrophic lateral sclerosis induces mitochondrial alteration and increase of cytosolic Ca2+ concentration in transfected neuroblastoma SH-SY5Y cells.

    abstract::We have set up a model system for familial amyotrophic lateral sclerosis (FALS) by transfecting human neuroblastoma cell line SH-SY5Y with plasmids directing constitutive expression of either wild-type human Cu,Zn superoxide dismutase (Cu,ZnSOD) or a mutant of this enzyme (G93A) associated with FALS. We have tested mi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Carrì MT,Ferri A,Battistoni A,Famhy L,Gabbianelli R,Poccia F,Rotilio G

    更新日期:1997-09-08 00:00:00