Abstract:
:The nuclear gene OXA1 was first isolated in Saccharomyces cerevisiae and found to be required at a post-translational step in cytochrome c oxidase biogenesis, probably at the level of assembly. Mutations in OXA1 lead to a complete respiratory deficiency. The protein Oxa1p is conserved through evolution and a human homolog has been isolated by functional complementation of a yeast oxa1- mutant. In order to further our understanding of the role of Oxa1p, we have constructed two yeast strains in which the OXA1 open reading frame was almost totally deleted. Cytochrome spectra and enzymatic activity measurements show the absence of heme aa3 and of a cytochrome c oxido-reductase activity and dramatic decrease of the oligomycin sensitive ATPase activity. Analysis of the respiratory complexes in non-denaturing gels reveals that Oxa1p is necessary for the correct assembly of the cytochrome c oxidase and the ATP synthase complex.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Altamura N,Capitanio N,Bonnefoy N,Papa S,Dujardin Gdoi
10.1016/0014-5793(96)00165-2subject
Has Abstractpub_date
1996-03-11 00:00:00pages
111-5issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(96)00165-2journal_volume
382pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Amylin, a 37 amino acid product of pancreatic beta-cells, inhibits insulin-stimulated radioglucose incorporation into muscle glycogen. In the current study, we exercised rats and then prelabelled the glycogen pool by infusing [3-3H]glucose along with glucose and insulin. Subsequent amylin administration increased the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80702-v
更新日期:1993-11-22 00:00:00
abstract::USP4, 11 and 15 are three closely related paralogues of the ubiquitin specific protease (USP) family of deubiquitinating enzymes. The DUSP domain and the UBL domain in these proteins are juxtaposed which may provide a functional unit conferring specificity. We determined the structures of the USP15 DUSP-UBL double dom...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.09.040
更新日期:2011-11-04 00:00:00
abstract::The sequence of seminalplasmin, a basic antimicrobial and transcription-inhibitory protein from bovine seminal plasma, has been determined using an automated sequenator. This sequence is slightly different from that reported earlier by Theil and Scheit [(1983) EMBO J. 2, 1159-1163] and identical with that of caltrin, ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80615-9
更新日期:1986-06-09 00:00:00
abstract::RNA binding motif protein 5 (RBM5) is a candidate tumor suppressor gene. Recent studies showed that RBM5 functions as an alternative splicing regulator of apoptosis-related genes. Here, we identify DHX15 and PRP19, two spliceosome components, as novel RBM5-interacting partners. We then show that the G-patch domain of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.02.052
更新日期:2012-04-05 00:00:00
abstract::Replication of Brucella inside eukaryotic cells is essential for pathogenesis, and successful infection requires rapid adaptation to the intracellular milieu. Close relatives of Brucella use the two-component system FixLJ to survive inside the host. We aimed to identify a homologous sensor in Brucella abortus. A predi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.07.047
更新日期:2013-09-17 00:00:00
abstract::The structural characteristics of the three nuclear phosphoproteins of the high mobility group A family are outlined and related to their participation in chromatin structure alteration in many biological processes such as gene expression, neoplastic transformation, differentiation, and apoptosis. The elevated express...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2004.08.013
更新日期:2004-09-10 00:00:00
abstract::Cellular homeostasis is linked tightly to mitochondrial functions. Some damage to mitochondrial proteins and nucleic acids can lead to the depolarization of the inner mitochondrial membrane, thereby sensitizing impaired mitochondria for selective elimination by autophagy. Mitochondrial dysfunction is one of the key as...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.02.060
更新日期:2010-04-02 00:00:00
abstract::The cDNA of the human muscle type acylphosphatase was isolated and characterized. The mRNA presents a very long 5'-untranslated region, covering the first half of the molecule: 175 bases of this part were cloned and prediction of the possible secondary structure showed that a very stable stem-loop structure could be f...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01270-2
更新日期:1997-11-03 00:00:00
abstract::With the aging of population, neurological disorders, and especially disorders involving defects in protein conformation (also known as proteopathies) pose a serious socio-economic problem. So far there is no effective treatment for most proteopathies, including Parkinson's disease (PD). The mechanism underlying PD pa...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.13464
更新日期:2019-07-01 00:00:00
abstract::Polyhydroxyalkanoate (PHA) synthases catalyze the polymerization reaction of the acyl moiety of hydroxyacyl-coenzyme A into polyester. The catalytic subunit PhaC of PHA synthase has the PhaC box sequence at the active site that is typically described as G-X-C-X-G-G (X is an arbitrary amino acid), and cysteine is an ac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13651
更新日期:2020-02-01 00:00:00
abstract::We have found that the sign of phototaxis, i.e. the direction of phototactic migration either towards or away from the light source, of wild-type Chlamydomonas reinhardtii depends on its photosynthetic activity. This accounts for the frequently reported transient positive phototaxis that precedes a negative phototaxis...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80867-t
更新日期:1993-12-28 00:00:00
abstract::Two major embryonic myosin heavy chains are expressed in embryonic chick breast muscle until the first week after hatching. Of these, one is already detected in the 8-day-old embryo. The other appears on day 12. Two putative slow embryonic isoforms represent minor components transiently expressed between days 8 and 12...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80488-5
更新日期:1988-10-10 00:00:00
abstract::The voltage-dependent calcium channel (VDCC) in skeletal muscle probably plays a key role in transducing membrane charge movement to the calcium release channel. We report here that the expression of VDCC alpha 1 and alpha 2 mRNAs is developmentally regulated in differentiating C2C12 myogenic cells. The alpha 1 mRNA i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80787-2
更新日期:1989-07-03 00:00:00
abstract::Ballistic transfection, based on cell and tissue bombardment by the tungsten and gold microparticles covered with the gene DNA, was used for the delivery of a bacterial beta-galactosidase and a full-length cDNA copy of the human dystrophin genes into mouse skeletal muscles. CMV-lacZ, SV40-lacZ, LTR-lacZneo and full-le...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01019-3
更新日期:1997-09-08 00:00:00
abstract::We examined the role of p38 mitogen-activated protein (MAP) kinase in the tumor necrosis factor alpha (TNF-alpha)- or interleukin-1beta (IL-1beta)-induced production of interleukin-6 (IL-6) and interleukin-8 (IL-8) in fresh rheumatoid synovial fibroblast (RSF) cultures concomitantly with the induction of p38 MAP kinas...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01717-2
更新日期:2000-01-07 00:00:00
abstract::The role of adenosine 5'-triphosphate (ATP) and P2Y(1) nucleotide receptor in potentiating agrin-induced acetylcholine receptor (AChR) aggregation is being demonstrated in a co-culture system of NG108-15 cell, a mouse neuroblastoma X rat glioma hybrid cell line that resembles spinal motor neuron, with C2C12 myotube. I...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.03.054
更新日期:2005-04-25 00:00:00
abstract::Long noncoding RNA (lncRNA) are emerging as important regulators of diverse biological functions. Although mechanistic models are starting to emerge, it is also clear that the lncRNA field needs appropriate model systems in order to better elucidate the functions of lncRNA and their roles in both physiological and pat...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12308
更新日期:2016-08-01 00:00:00
abstract::Three site-directed mutants of human epidermal growth factor, Leu-26----Gly, Leu-47----Ala, and Ile-23----Thr, were examined for their ability to stimulate the protein-tyrosine kinase activity of the epidermal growth factor receptor. The receptor binding affinities of the mutant growth factors were 20- to 50-fold lowe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80776-f
更新日期:1990-05-07 00:00:00
abstract::Rat calvaria cells grown in culture for one week had properties of osteoblasts: a high content in alkaline phosphatase and a marked cyclic AMP response to parathyroid hormone (PTH). In short-term experiments, insulin-like growth factor I (IGF I) stimulated the incorporation of [14C] glucose into glycogen. When IGF I w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81015-7
更新日期:1984-07-23 00:00:00
abstract::Calmodulin is shown to interact with human spectrin dimer. The binding was highly calcium-dependent and observed in two different kinds of experiments. Firstly, affinity chromatography of calmodulin on a Sepharose 4B column with immobilized spectrin, and secondly, partition in aqueous two-phase polymer systems. In the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80884-4
更新日期:1984-06-25 00:00:00
abstract::Platelet release products and purified platelet-derived growth factor stimulated the phosphorylation of ribosomal protein S6 in cultured mouse Balb/c 3T3 fibroblasts. The post-nuclear fraction of the stimulated cells was enriched in S6 kinase activity specific for sites resembling those phosphorylated within intact ce...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81072-2
更新日期:1983-10-03 00:00:00
abstract::The fluorescence intensity and anisotropy decays of the intrinsic tryptophan emission from six Fe/S proteins (ranging from the very simplest ones to enzyme complexes containing one, two or more Trp residues) were measured. All proteins were examined in the reduced and the oxidized state. In either redox state each pro...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00606-7
更新日期:1994-07-18 00:00:00
abstract::Dried cells of Brevibacterium ammoniagenes are a good enzyme source for the preparative synthesis of CoA from pantothenic acid, L-cysteine and ATP. A problem with this synthesis is that the CoA synthesis is repressed by negative feedback inhibition by CoA to pantothenate kinase, the first step enzyme for the biosynthe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80091-x
更新日期:1983-01-24 00:00:00
abstract::Methyl-beta-cyclodextrin (MbetaCD) was used to explore a role for cholesterol-enriched plasma membrane microdomains in coupling lysophosphatidic acid (LPA) stimulation to phosphoinositide 3-kinase (PI3K) activation. Cholesterol depletion strongly inhibited the production of phosphatidylinositol 3,4-bisphosphate and ph...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03832-2
更新日期:2003-01-16 00:00:00
abstract::Oncogenic mutations disrupt the regulatory circuits that govern cell function, enabling tumor cells to undergo de-regulated mitogenesis, to resist to pro-apoptotic insults, and to invade through tissue boundaries. Cancer cell biology has played a crucial role in elucidating the signaling mechanisms by which oncogenic ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2014.02.005
更新日期:2014-08-19 00:00:00
abstract::It was determined that flavan-3-ols and procyanidins have an inhibitory effect on angiotensin I converting enzyme (ACE) activity, and the effect was dependent on the number of epicatechin units forming the procyanidin. The inhibition by flavan-3-ols and procyanidins was competitive with the two substrates assayed: N-h...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01355-3
更新日期:2003-12-18 00:00:00
abstract::Currently described substrates of the bacterial Tat protein transport system are directed for export by signal peptides containing a pair of invariant arginine residues. The signal peptide of the TtrB subunit of Salmonella enterica tetrathionate reductase contains a single arginine residue but is nevertheless able to ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02428-0
更新日期:2001-05-18 00:00:00
abstract::Protein kinase C (PKC) from human promyelocytic leukemia HL-60 cells can be resolved into three fractions (peak, a, b and c) by hydroxyapatite column chromatography. Peak a and c enzymes are indistinguishable from the brain type II PKC having beta (beta I and beta II)-sequence and type III having alpha-sequence, respe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80698-i
更新日期:1990-04-09 00:00:00
abstract::ATP synthesis rate, measured as the variation in external Pi concentration, varied as a linear function of either delta mu H+ or delta Gpin, in such a manner that the delta Gpin/delta mu H+ ratio increased while VATP increased. We also observed a linear dependence of the flux control coefficient of the Pi carrier on...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80539-3
更新日期:1989-02-27 00:00:00
abstract::We have set up a model system for familial amyotrophic lateral sclerosis (FALS) by transfecting human neuroblastoma cell line SH-SY5Y with plasmids directing constitutive expression of either wild-type human Cu,Zn superoxide dismutase (Cu,ZnSOD) or a mutant of this enzyme (G93A) associated with FALS. We have tested mi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01051-x
更新日期:1997-09-08 00:00:00