Identification of possible adenine nucleotide-binding sites in nitrogenase Fe- and MoFe-proteins by amino acid sequence comparison.

abstract：:Mycobacterium tuberculosis contains multiple versions of the accA and accD genes that encode the alpha- and beta-subunits of at least three distinct multi-functional acyl-CoA carboxylase complexes. Because of its proposed involvement in pathogenic M. tuberculosis survival, the high-resolution crystal structure of the ...

journal_title：FEBS letters

authors： Holton SJ,King-Scott S,Nasser Eddine A,Kaufmann SH,Wilmanns M

更新日期：2006-12-22 00:00:00

abstract：:Treatment of the myeloid cell lines, U-937 or HL-60, with 10 nM of the phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), for 24 h increased the rate of incorporation of [3H]glycerol into total chloroform extracts. A proportionally greater labeling of the non-polar lipid (NL) fraction compared to the polar, ph...

journal_title：FEBS letters

authors： Ponzoni M,DeBell KE,Hoffman T,Bonvini E

更新日期：1990-12-10 00:00:00

abstract：:Various molecular mechanisms are involved in the efficacy of arsenic trioxide (ATO) against malignant hematologic and some solid tumors. FLICE-like inhibitory protein (FLIP) is an inhibitor of apoptosis mediated by death receptors. In this study, we identified a new link between the down-regulation of cellular FLIP(L)...

journal_title：FEBS letters

authors： Zhang G,Liu J,Zhang Y,Qu J,Xu L,Zheng H,Liu Y,Qu X

更新日期：2012-09-21 00:00:00

abstract：:PDZ domains are thought to act as protein-binding modules mediating the clustering of membrane and membrane-associated proteins. The INAD protein has been shown to interact via a PDZ domain with the calcium channel TRP which contributes to capacitative calcium entry into Drosophila photoreceptor cells. We have cloned ...

journal_title：FEBS letters

authors： Philipp S,Flockerzi V

更新日期：1997-08-18 00:00:00

abstract：:Antithrombin Glasgow is a hereditary abnormal antithrombin that has lost thrombin inhibitory activity. It was isolated from the plasma of a 41-year-old male with a history of thrombotic events. Antithrombin Glasgow was purified from plasma using heparin-Sepharose chromatography at pH 7.4 eluting with increasing concen...

journal_title：FEBS letters

authors： Owen MC,Beresford CH,Carrell RW

更新日期：1988-04-25 00:00:00

abstract：:The YukD protein in Bacillus subtilis was identified in a hidden Markov model (HMM) search as being related in sequence to ubiquitin. By solving the crystal structure we show that YukD adopts a fold that is most closely related to ubiquitin, yet has the shortest C-terminal tail of all known ubiquitin-like proteins. Th...

journal_title：FEBS letters

authors： van den Ent F,Löwe J

更新日期：2005-07-04 00:00:00

abstract：:The yeast integral plasma membrane protein Ist2 belongs to a group of membrane proteins which are synthesized from localized mRNAs. The protein reaches the plasma membrane via the ER on a route operating independently of the classical secretory pathway. We have identified a complex peptide-sorting signal located at th...

journal_title：FEBS letters

authors： Franz A,Maass K,Seedorf M

更新日期：2007-02-06 00:00:00

abstract：:The integrin alpha IIb beta 3-mediated redistribution of the tyrosine kinases pp125FAK and pp60Src and the small GTP-binding proteins CDC42Hs and Rap1B from the membrane skeleton to the cytoskeleton was found to be reversible: upon prolonged platelet aggregation (up to 15 min) induced by the thrombin-receptor activati...

journal_title：FEBS letters

authors： Dash D,Aepfelbacher M,Siess W

更新日期：1995-04-24 00:00:00

abstract：:Primary cultures of rat dorsal root ganglionic (DRG) cells were stained with isoform-specific antibodies against non-muscle myosin II. Antibodies against the brain type myosin (MIIB) stained the peripheries of growth cones and non-neuronal cells. Double staining of the cells with the anti-myosin antibodies and rhodami...

journal_title：FEBS letters

authors： Cheng TP,Murakami N,Elzinga M

更新日期：1992-10-19 00:00:00

abstract：:Biotic and abiotic stress induces the formation of galactolipids esterified with the phytohormones 12-oxo-phytodienoic acid (OPDA) and dinor-oxo-phytodienoic acid (dnOPDA) in Arabidopsis thaliana. The biosynthetic pathways of free (dn)OPDA is well described, but it is unclear how they are incorporated into galactolipi...

journal_title：FEBS letters

authors： Nilsson AK,Fahlberg P,Ellerström M,Andersson MX

更新日期：2012-07-30 00:00:00

abstract：:The role of Na+/H+ exchange in protein kinase C-mediated effects in platelets was investigated by studying the effect of removal of extracellular Na+ ([Na+]e) on the different responses induced by phorbol 12-myristate 13-acetate (PMA) and 1,2-dioctanoylglycerol (diC8). None of the responses studied, namely, protein ph...

journal_title：FEBS letters

authors： Krishnamurthi S,Patel Y,Morgan WA,Wheeler-Jones CP,Kakkar VV

更新日期：1989-07-31 00:00:00

abstract：:NDRG1 is phosphorylated by SGK1 (but not PKB) in vivo at three residues each contained within three nonapeptide repeats. Here, we demonstrate that this nonapeptide, like the NDRG1 protein, is phosphorylated by SGK1, but not by PKBalpha or RSK1 in vitro. The inability of PKBalpha and RSK1 to phosphorylate the nonapepti...

journal_title：FEBS letters

authors： Murray JT,Cummings LA,Bloomberg GB,Cohen P

更新日期：2005-02-14 00:00:00

abstract：:We have used a diethylpyrocarbonate (DEPC) modification [(1976) Prog. Nucl. Acids Res. 16, 189-262] to probe the accessibility of adenines essential for coat protein binding in the MS2 translational operator [(1983) Biochemistry 22, 2601-2610, 2610-2615, 4723-4730; (1987) Biochemistry 26, 1563-1568]. The essential ade...

journal_title：FEBS letters

authors： Talbot SJ,Medina G,Fishwick CW,Haneef I,Stockley PG

更新日期：1991-05-20 00:00:00

abstract：:The cholecystokinin (CCK) receptor in purified plasma membranes prepared from mouse pancreatic acini had a binding affinity of 1.8 nM, an acid pH optimum between 6.0 and 6.5, and an analog specificity of CCK8 greater than CCK33 greater than desulphated CCK8 greater than CCK4. Binding of CCK to its receptor was abolish...

journal_title：FEBS letters

authors： Sakamoto C,Williams JA,Wong KY,Goldfine ID

更新日期：1983-01-10 00:00:00

abstract：:Mediatophore is a protein purified from the nerve terminal membranes of Torpedo electric organ. It confers to artificial membranes a calcium-dependent mechanism that translocates acetylcholine. When similar reconstitution experiments are applied to rat brain synaptosomal membranes they reveal the presence of mediatoph...

journal_title：FEBS letters

authors： Israël M,Lesbats B,Morel N,Manaranche R,Le Gal la Salle G

更新日期：1988-06-20 00:00:00

abstract：:Using a phage display peptide library, we characterized the epitope of two monoclonal antibodies reacting with syndecan-1: B-B2 and B-B4. The identified epitopes QDIT, for B-B2, and LPEV, for B-B4, were found to align with residues 36-39 and 90-93 of the mature protein, respectively. In contrast to B-B4, the B-B2 epit...

journal_title：FEBS letters

authors： Dore JM,Morard F,Vita N,Wijdenes J

更新日期：1998-04-10 00:00:00

abstract：:Homologues of the catalase-peroxidase gene katG and the gene for the non-specific DNA binding protein dpsA were identified downstream of oxyR in Burkholderia pseudomallei. Northern experiments revealed that both katG and dpsA are co-transcribed during oxidative stress. Under conditions where the katG promoter is not h...

journal_title：FEBS letters

authors： Loprasert S,Whangsuk W,Sallabhan R,Mongkolsuk S

更新日期：2003-05-08 00:00:00

abstract：:Data from a number of model systems support a role for proteolysis in apoptotic cell death. Using immature rat thymocytes, we demonstrate that the inhibitors N-tosyl-L-lysyl chloromethylketone (TLCK) and N-tosyl-L-phenylalanyl chloromethylketone (TPCK) have very different effects on apoptosis. TLCK inhibits apoptosis ...

journal_title：FEBS letters

authors： Fearnhead HO,Rivett AJ,Dinsdale D,Cohen GM

更新日期：1995-01-09 00:00:00

abstract：:The O-chain polysaccharide (O-PS) of Bordetella avium was isolated from the lipopolysaccharide by mild acid hydrolysis to remove the lipid A, followed by hydrofluorolysis to remove the lipopolysaccharide core oligosaccharide leaving a residual O-PS for structural analysis. High resolution (1)H and (13)C NMR and MALDI ...

journal_title：FEBS letters

authors： Larocque S,Brisson JR,Thérisod H,Perry MB,Caroff M

更新日期：2003-01-30 00:00:00

abstract：:Placental alkaline phosphatase is an inducible enzyme, expressed in HeLaS3 cells, which has been shown to possess protein phosphotyrosine phosphatase activity. Since phosphotyrosine levels are known to increase in actively dividing cells we sought an inverse correlation between PLAP activity and growth rate in HeLaS3 ...

journal_title：FEBS letters

authors： Telfer JF,Green CD

更新日期：1993-08-30 00:00:00

abstract：:In the first committed reaction of chlorophyll biosynthesis, magnesium chelatase couples ATP hydrolysis to the thermodynamically unfavorable Mg(2+) insertion into protoporphyrin IX (ΔG°' of circa 25-33 kJ·mol(-1) ). We explored the thermodynamic constraints on magnesium chelatase and demonstrate the effect of nucleoti...

journal_title：FEBS letters

authors： Adams NB,Brindley AA,Hunter CN,Reid JD

更新日期：2016-06-01 00:00:00

abstract：:o-Aminophenol was found to be rapidly metabolized to a brown compound in the presence of purified human oxy- and methemoglobin, coupled with the oxidation and reduction of these hemoglobins by o-aminophenol. The final product of o-aminophenol was identified as 2-aminophenoxazine-3-one, by using spectrophotometry and H...

journal_title：FEBS letters

authors： Tomoda A,Yamaguchi J,Kojima H,Amemiya H,Yoneyama Y

更新日期：1986-02-03 00:00:00

abstract：:Incubation of adipocytes with 125I-insulin plus leupeptin or monensin, but not chloroquine, resulted in the appearance of a novel peak of 125I-insulin (modal density about 1.20 g/ml) on density gradient centrifugation; the appearance of the peak depended on the presence of specific insulin receptors on the cell surfac...

journal_title：FEBS letters

authors： Jeffrey IW,Flint DJ,Vernon RG,Mayer RJ

更新日期：1985-03-25 00:00:00

abstract：:A high-throughput cell-free protein synthesis method has been described. The methodology is based on a bilayer diffusion system that enables the continuous supply of substrates, together with the continuous removal of small byproducts, through a phase between the translation mixture and substrate mixture. With the use...

journal_title：FEBS letters

authors： Sawasaki T,Hasegawa Y,Tsuchimochi M,Kamura N,Ogasawara T,Kuroita T,Endo Y

更新日期：2002-03-06 00:00:00

abstract：:Protein D2 forms the water-filled pore across the outer membrane of Pseudomonas aeruginosa and allows the penetration of imipenem. We cloned the protein D2 gene by the antibody screening technique. When the imipenem-resistant mutant lacking protein D2 harbored the plasmid with the cloned D2 gene, the mutant overproduc...

journal_title：FEBS letters

authors： Yoneyama H,Nakae T

更新日期：1991-06-03 00:00:00

abstract：:Transglutaminase 2 (TG2) is a GTP-binding protein with transglutaminase activity. Despite advances in the characterization of TG2 functions and their impact on cellular processes, the role of TG2 in Human chronic myelogenous leukemia K562 cell line is still poorly understood. To understand the biological significance ...

journal_title：FEBS letters

authors： Kang SK,Lee JY,Chung TW,Kim CH

更新日期：2004-11-19 00:00:00

abstract：:We investigated 38 cases of B-cell chronic lymphocytic leukemia (B-CLL) for the presence of non-productive rearrangements in the S(mu) regions and defined for the first time the molecular nature of these rearrangements. Southern blot analysis revealed S(mu) region rearrangements in 13 cases (34%) and polymerase chain ...

journal_title：FEBS letters

authors： Nardini E,Rizzi S,Capello D,Vitolo U,Gaidano G,Menard S,Balsari A

更新日期：2002-05-08 00:00:00

abstract：:Insight into the regulation of the actions of the protein-tyrosine kinases will be greatly facilitated by the full characterization of the family of protein-tyrosine phosphatases. A search for novel phosphatases resulted in the isolation of a cDNA, termed HLPR, encoding a member of the family of human receptor-like pr...

journal_title：FEBS letters

authors： Jirik FR,Janzen NM,Melhado IG,Harder KW

更新日期：1990-10-29 00:00:00

abstract：:The β-xylosidase B from Bifidobacterium adolescentis ATCC15703 belongs to the newly characterized family 120 of glycoside hydrolases. In order to investigate its catalytic mechanism, an extensive kinetic study of the wild-type enzyme and mutants targeting the three highly conserved residues Asp(393), Glu(416) and Glu(...

journal_title：FEBS letters

authors： Cecchini DA,Fauré R,Laville E,Potocki-Veronese G

更新日期：2015-10-07 00:00:00

abstract：:Coronin proteins are widely expressed among eukaryotic organisms. Most coronins consist of a WD-repeat domain followed by a C-terminal coiled coil. Dictyostelium discoideum expresses a single short coronin coronin A, which has been implicated in both actin modulation and multicellular differentiation. Whether coronin ...

journal_title：FEBS letters

authors： Fiedler T,Fabrice TN,Studer V,Vinet A,Faltova L,Kammerer RA,Steinmetz MO,Sharpe T,Pieters J

更新日期：2020-04-16 00:00:00