Placental alkaline phosphatase activity is inversely related to cell growth rate in HeLaS3 cervical cancer cells.

abstract：:The ANKRD1/CARP gene encodes a muscle-specific protein which has been implicated in transcriptional regulation and myofibrillar assembly. Several features at both the mRNA and protein levels define ANKRD1 as a gene whose expression is tightly regulated, and deregulated expression of this protein has been recently asso...

journal_title：FEBS letters

authors： Badi I,Cinquetti R,Frascoli M,Parolini C,Chiesa G,Taramelli R,Acquati F

更新日期：2009-08-06 00:00:00

abstract：:Recombinant porcine growth hormone (rPGH) was solubilised from inclusion bodies (IB's) using either 6 M guanidinium hydrochloride (GnHCl), 7.5 M urea or by a novel method using a cationic surfactant, cetyltrimethylammonium chloride (CTAC). Circular dichroism (CD) analysis of the secondary (2 degrees) structure of the ...

journal_title：FEBS letters

authors： Puri NK,Cardamone M

更新日期：1992-07-06 00:00:00

abstract：:A novel protein that binds specifically to the intracellular domain of the p55 tumor necrosis factor (TNF) receptor was cloned by two-hybrid screening of a HeLa cell cDNA library. Data bank searches revealed high sequence similarity of the protein (55.11) to yeast, nematode and plant proteins, whose functions are yet ...

journal_title：FEBS letters

authors： Boldin MP,Mett IL,Wallach D

更新日期：1995-06-19 00:00:00

abstract：:Metal-binding peptides in proteolytic digest maps have been identified by matrix-assisted UV laser desorption time-of-flight mass spectrometry (LDTOF-MS). The plasma and milk metal transport protein chosen to demonstrate this process, histidine-rich glycoprotein (HRG), was purified and then digested with trypsin; the ...

journal_title：FEBS letters

authors： Hutchens TW,Nelson RW,Yip TT

更新日期：1992-01-13 00:00:00

abstract：:The effect of the inositol phospholipid-binding antibiotic neomycin was studied on high-affinity GTPase in human platelet membranes. At low concentrations (up to 1 mM), neomycin by itself stimulated a high-affinity GTPase. This GTPase stimulation was additive with that caused by the hormonal factors, prostaglandin E1 ...

journal_title：FEBS letters

authors： Herrmann E,Jakobs KH

更新日期：1988-02-29 00:00:00

abstract：:Comparative allelotyping of the short arm of human chromosome 3 (3p) in four types of epithelial carcinomas was performed using an identical set of polymorphic markers. In total, 117 samples of non-papillary renal cell carcinoma (RCC), non-small cell lung carcinoma (NSCLC), carcinoma of uterine cervix (CC), and breast...

journal_title：FEBS letters

authors： Braga E,Pugacheva E,Bazov I,Ermilova V,Kazubskaya T,Mazurenko N,Kisseljov F,Liu J,Garkavtseva R,Zabarovsky E,Kisselev L

更新日期：1999-07-09 00:00:00

abstract：:Sarafotoxins (SRTa, SRTb and SRTc) as well as endothelin-1 (ET-1) produced vasoconstrictions in rat thoracic aorta, rat isolated perfused mesentery and pithed rat in various of extents. The potency was ET-1 greater than SRTb greater than SRTa greater than SRTc at lower doses, but SRTb greater than ET-1 greater than SR...

journal_title：FEBS letters

authors： Kitazumi K,Shiba T,Nishiki K,Furukawa Y,Takasaki C,Tasaka K

更新日期：1990-01-29 00:00:00

abstract：:Protamines are polycationic proteins that are widely used for neutralisation of the anticoagulant action of heparin. However, several reports have shown adverse, mast cell-dependent reactions to protamine. The exact mechanism by which protamine causes these adverse effects is not clear. In the present study, the possi...

journal_title：FEBS letters

authors： Pejler G

更新日期：1996-04-01 00:00:00

abstract：:6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase isoform 3 (PFKFB3) is a key enzyme of the glycolytic pathway, and it plays an essential role in angiogenesis. 3-(3-Pyridinyl)-1-(4-pyridinyl)-2-propen-1-one (3PO) is frequently used as a glycolysis inhibitor and is thought to inhibit PFKFB3. However, this latter eff...

journal_title：FEBS letters

authors： Emini Veseli B,Perrotta P,Van Wielendaele P,Lambeir AM,Abdali A,Bellosta S,Monaco G,Bultynck G,Martinet W,De Meyer GRY

更新日期：2020-09-01 00:00:00

abstract：:The phosphatidylinositol 3-kinase/Akt pathway is responsible for key aspects of tumor progression, and is frequently hyperactivated in cancer. We have recently identified palladin, an actin-bundling protein that functions to control the actin cytoskeleton, as an Akt1-specific substrate that inhibits breast cancer cell...

journal_title：FEBS letters

authors： Chin YR,Toker A

更新日期：2010-12-01 00:00:00

abstract：:Agonist-induced changes in expression and mRNA levels of luteinizing hormone (LH) receptors were compared during stimulation of ovarian follicular maturation and luteinization by gonadotropic hormones. Three major species of LH receptor mRNA, 5.8, 2.6 and 2.3 kb, were present throughout differentiation and changed sim...

journal_title：FEBS letters

authors： Hu ZZ,Tsai-Morris CH,Buczko E,Dufau ML

更新日期：1990-11-12 00:00:00

abstract：:The folding of HIV gp41 into a 6-helix bundle drives virus-cell membrane fusion. To examine the structural relationship between the 6-helix bundle core domain and other regions of gp41, we expressed in Escherichia coli, the entire ectodomain of HIV-2(ST) gp41 as a soluble, trimeric maltose-binding protein (MBP)/gp41 c...

journal_title：FEBS letters

authors： Lay CS,Wilson KA,Kobe B,Kemp BE,Drummer HE,Poumbourios P

更新日期：2004-06-04 00:00:00

abstract：:A lipid-binding protein identical to the rat intestinal bile acid-binding protein, termed I-15P, was expressed in steroid hormone-producing tissues such as ovary and adrenal gland, but not testis. In immature rats, I-15P was expressed in intestine but not in ovaries. The expression of I-15P in the ovaries of immature ...

journal_title：FEBS letters

authors： Sato E,Fujii H,Fujita M,Kanda T,Iseki S,Hatakeyama K,Tanaka T,Ono T

更新日期：1995-10-30 00:00:00

abstract：:Buthionine sulfoximine (BSO) induces decreased glutathione (GSH) and trypanothione [T(SH)2 ] pools in trypanosomatids, presumably because only gamma-glutamylcysteine synthetase (γECS) is blocked. However, some BSO effects cannot be explained by exclusive γECS inhibition; therefore, its effect on the T(SH)2 metabolism ...

journal_title：FEBS letters

authors： Vázquez C,Mejia-Tlachi M,González-Chávez Z,Silva A,Rodríguez-Zavala JS,Moreno-Sánchez R,Saavedra E

更新日期：2017-12-01 00:00:00

abstract：:As a result of Myc-dependent transcription of the LDH-A gene, Myc-transformed cells (Rat1-Myc) exhibit increased lactate production rates (LPR) even under aerobic conditions (the Warburg effect). Recently, the increased susceptibility to stress-induced apoptosis associated with Myc transfection has been linked to the ...

journal_title：FEBS letters

authors： Papas KK,Sun L,Roos ES,Gounarides JS,Shapiro M,Nalin CM

更新日期：1999-03-12 00:00:00

abstract：:A recombinant expression system was developed to analyse sequence determinants involved in O-glycosylation of proteins in mycobacteria. By expressing peptide sequences corresponding to known glycosylation sites within a chimeric lipoprotein construct, amino acids flanking modified threonine residues were found to have...

journal_title：FEBS letters

authors： Herrmann JL,Delahay R,Gallagher A,Robertson B,Young D

更新日期：2000-05-19 00:00:00

abstract：:Mitochondrial uncoupling protein 2 (UCP2) is abundant in developing monocyte/macrophage cells and may affect hematopoiesis by reducing formation of reactive oxygen species. The aims of this study were to further characterize the involvement of UCP2 in hematopoiesis. In situ hybridization in mouse embryos identified UC...

journal_title：FEBS letters

authors： Flachs P,Sponarova J,Kopecky P,Horvath O,Sediva A,Nibbelink M,Casteilla L,Medrikova D,Neckar J,Kolar F,Kopecky J

更新日期：2007-03-20 00:00:00

abstract：:To understand the role of nitric oxide (NO) in controlling the specific DNA-binding activities of transcriptional factors, we investigated the in vitro effect of the NO-donor sodium nitroprusside (SNP) on the AP-1 activity of cultured mouse cerebellar granule cells. A gel-mobility assay showed that SNP inhibited AP-1 ...

journal_title：FEBS letters

authors： Tabuchi A,Sano K,Oh E,Tsuchiya T,Tsuda M

更新日期：1994-08-29 00:00:00

abstract：:Three regions of the alpha chain of Torpedo californica acetylcholine receptor (AChR), corresponding to residues alpha 262-276, alpha 388, 408 and alpha 427-437 were synthesized, purified and characterized. The first two peptides have been proposed to occupy inter-transmembrane regions while the third represented the ...

journal_title：FEBS letters

authors： Atassi MZ,Manshouri T,Yokoi T

更新日期：1988-02-15 00:00:00

abstract：:PsbU is one of the extrinsic proteins in red algal Photosystem II (PSII) and functions to optimize the availability of Ca(2+) and Cl(-) cofactors for water oxidation. To determine the functional residue of PsbU, we constructed various PsbU mutants from a red alga Cyanidium caldarium and reconstituted these mutants wit...

journal_title：FEBS letters

authors： Okumura A,Sano M,Suzuki T,Tanaka H,Nagao R,Nakazato K,Iwai M,Adachi H,Shen JR,Enami I

更新日期：2007-11-13 00:00:00

abstract：:Gangliosides and neutral glycolipids of muscles from normal and dystrophic chickens were studied. Total glycolipid content of the degenerating muscles was higher than the normal muscles. In addition, the myopathic muscles contained a ganglioside which was absent in the unaffected muscles from normal and dystrophic chi...

journal_title：FEBS letters

authors： Kundu SK,Misra LK,Luthra MG

更新日期：1982-12-27 00:00:00

abstract：:The spike (S) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) is an important viral structural protein. Based on bioinformatics analysis, 10 antigenic peptides derived from the S protein sequence were selected and synthesized. The antigenicity and immunoreactivity of all the peptides were tested in...

journal_title：FEBS letters

authors： Lu W,Wu XD,Shi MD,Yang RF,He YY,Bian C,Shi TL,Yang S,Zhu XL,Jiang WH,Li YX,Yan LC,Ji YY,Lin Y,Lin GM,Tian L,Wang J,Wang HX,Xie YH,Pei G,Wu JR,Sun B

更新日期：2005-04-11 00:00:00

abstract：:xxR/KxRxxSxx sequences were phosphorylated with high efficiency by both p70 S6 kinase (p70S6K) and MAPKAP kinase-1. The best substrate for MAPKAP kinase-1 (KKKNRTLSVA) was phosphorylated with a Km of 0.17 microM, and the best substrate for p70S6K (KKRNRTLSVA) with a Km of 1.5 microM. The requirement of both enzymes fo...

journal_title：FEBS letters

authors： Leighton IA,Dalby KN,Caudwell FB,Cohen PT,Cohen P

更新日期：1995-11-20 00:00:00

abstract：:The prohormone convertase PC2 is one of the major subtilisin/kexin-like enzymes responsible for the formation of small bioactive peptides in neural and endocrine cells. This convertase is unique among the members of the subtilisin/kexin-like mammalian serine proteinase family in that it undergoes zymogen processing of...

journal_title：FEBS letters

authors： Benjannet S,Mamarbachi AM,Hamelin J,Savaria D,Munzer JS,Chrétien M,Seidah NG

更新日期：1998-05-22 00:00:00

abstract：:The two cytoskeletal proteins VASP and WASP and the protein Homer share a conserved domain, currently designated the WHI domain (WASP homology domain 1) or EVH1 domain (ENA/VASP homology domain 1), which could play an important role in various cellular events such as transport, folding of proteins, and signal transduc...

journal_title：FEBS letters

authors： Callebaut I,Cossart P,Dehoux P

更新日期：1998-12-18 00:00:00

abstract：:Purified antibodies against guanylic acid and guanosine binding to RNA at guanosine residues were used to probe human lymphocyte preparations by indirect immunofluorescence. Neither antibody gave any banding pattern with metaphase chromosomes but both showed binding to specific sites in the interphase nuclei. Evidence...

journal_title：FEBS letters

authors： Iswari S,Krishnamurthy DS,Jacob TM

更新日期：1985-09-23 00:00:00

abstract：:Decarboxylation of pyruvate by pyruvate decarboxylase (EC 4.1.1.1) was performed in a reaction mixture containing 50% deuterium. The isolated product, acetaldehyde, was investigated directly by 1H NMR and by mass spectrometry after conversion to the 2,4-dinitrophenyl hydrazone. The protium content of 56% at acetaldehy...

journal_title：FEBS letters

authors： Ermer J,Schellenberger A,Hübner G

更新日期：1992-03-09 00:00:00

abstract：:Lipid analyses were carried out on transplantable murine Friend leukemia cell tumors, 6 h after intratumoral administration of tumor necrosis factor-alpha (TNF). The levels of the major phospholipid classes were uniformly decreased to about 70% of control values; free fatty acids were increased to about 170%; diacylgl...

journal_title：FEBS letters

authors： Bravo E,Carpinelli G,Proietti E,Belardelli F,Cantafora A,Podo F

更新日期：1990-01-29 00:00:00

abstract：:Ischemia/reperfusion induces nuclear factor kappaB (NF-kappaB) and AP-1 in rat hearts after 15 min of ischemia followed by reperfusion (R) for various periods of time (15 and 30 min, 1, 2, 3, 6, 12, and 24 h). Low levels of NF-kappaB and no signal for AP-1 were detected in shams and in non-ischemic tissue distant from...

journal_title：FEBS letters

authors： Chandrasekar B,Freeman GL

更新日期：1997-01-13 00:00:00

abstract：:HMr alpha 2CPI was found to be an inhibitor of human liver cathepsin H by the measurement of the dissociation constant (Ki), the association rate constant (k1) and the dissociation rate constant (k-1) between the enzyme and the inhibitor. These data suggest that this protein-proteinase inhibitor can play a physiologic...

journal_title：FEBS letters

authors： Pagano M,Engler R

更新日期：1984-01-23 00:00:00