Abstract:
:Biotic and abiotic stress induces the formation of galactolipids esterified with the phytohormones 12-oxo-phytodienoic acid (OPDA) and dinor-oxo-phytodienoic acid (dnOPDA) in Arabidopsis thaliana. The biosynthetic pathways of free (dn)OPDA is well described, but it is unclear how they are incorporated into galactolipids. We herein show that (dn)OPDA containing lipids are formed rapidly after disruption of cellular integrity in leaf tissue. Five minutes after freeze-thawing, 60-70% of the trienoic acids esterified to chloroplast galactolipids are converted to (dn)OPDA. Stable isotope labeling with (18)O-water provides strong evidence for that the fatty acids remain attached to galactolipids during the enzymatic conversion to (dn)OPDA.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Nilsson AK,Fahlberg P,Ellerström M,Andersson MXdoi
10.1016/j.febslet.2012.06.010subject
Has Abstractpub_date
2012-07-30 00:00:00pages
2483-7issue
16eissn
0014-5793issn
1873-3468pii
S0014-5793(12)00488-7journal_volume
586pub_type
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