A sensitive and specific radioimmunoassay for leukotriene C4.

abstract：:Syndecans are transmembrane heparan sulfate proteoglycans, with roles in development, tumorigenesis and inflammation, and growing evidence for involvement in tissue regeneration. This is a fast developing field with the prospect of utilizing tissue engineering and biomaterials in novel therapies. Syndecan receptors ar...

journal_title：FEBS letters

authors： Chung H,Multhaupt HA,Oh ES,Couchman JR

更新日期：2016-08-01 00:00:00

abstract：:Hydrin 2 (vasotocinyl-Gly), a hydroosmotic peptide resulting from differential processing of provasotocin and recently identified in frog neurohypophysis, has been looked for in the pituitary gland of an exotic toad (Bufo marinus) and of a reptile (Vipera aspis). Hydrin 2 has been found in the amphibian but not in the...

journal_title：FEBS letters

authors： Michel G,Rouillé Y,Chauvet MT,Chauvet J,Acher R

更新日期：1990-05-07 00:00:00

abstract：:The intermediate in the reaction catalyzed by 4-chlorobenzoate dehalogenase from Pseudomonas sp. CBS3 was identified as 4-chlorobenzoyl-CoA. One component of 4-chlorobenzoate dehalogenase worked as a a 4-chlorobenzoyl-CoA ligase catalyzing the formation of 4-chlorobenzoyl-CoA from 4-chlorobenzoate, coenzyme A and ATP....

journal_title：FEBS letters

authors： Löffler F,Müller R

更新日期：1991-09-23 00:00:00

abstract：:Although transthyretin (TTR) is expressed in pancreatic alpha (glucagon) cells in the islets of Langerhans, the function of TTR in pancreatic alpha cells remains unknown. In this study, by using TTR knockout (TTR KO) mice, we determined the novel role of TTR in glucose homeostasis. We demonstrated that TTR KO mice evi...

journal_title：FEBS letters

authors： Su Y,Jono H,Misumi Y,Senokuchi T,Guo J,Ueda M,Shinriki S,Tasaki M,Shono M,Obayashi K,Yamagata K,Araki E,Ando Y

更新日期：2012-11-30 00:00:00

abstract：:Hepatitis C virus (HCV) often establishes a persistent infection that leads to chronic liver diseases. The viral core protein modulates various cellular activities involved in this process. We found two mutations, K23E and V31A, in the core gene of the transfected HCV JFH-1 genome, which had been replicated for a prol...

journal_title：FEBS letters

authors： Kang JI,Kwon YC,Ahn BY

更新日期：2012-05-07 00:00:00

abstract：:The ANKRD1/CARP gene encodes a muscle-specific protein which has been implicated in transcriptional regulation and myofibrillar assembly. Several features at both the mRNA and protein levels define ANKRD1 as a gene whose expression is tightly regulated, and deregulated expression of this protein has been recently asso...

journal_title：FEBS letters

authors： Badi I,Cinquetti R,Frascoli M,Parolini C,Chiesa G,Taramelli R,Acquati F

更新日期：2009-08-06 00:00:00

abstract：:We solved the crystal structure of a functionally uncharacterized protein, HP0902, from Helicobacter pylori. Its structure demonstrated an all-β cupin fold that cannot bind metal ions due to the absence of a metal-binding histidine that is conserved in many metallo-cupins. In contrast, isothermal titration calorimetry...

journal_title：FEBS letters

authors： Sim DW,Kim JH,Kim HY,Jang JH,Lee WC,Kim EH,Park PJ,Lee KH,Won HS

更新日期：2016-09-01 00:00:00

abstract：:pMNEI, a single chain sweet protein related to monellin, has been studied by means of 1H NMR at 500 MHz. A partial sequential assignment performed by means of the MCD method allowed the determination of the secondary structure of a large portion of the beta-sheet of pMNEI that contains a likely 'sweet finger': the loo...

journal_title：FEBS letters

authors： Tancredi T,Iijima H,Saviano G,Amodeo P,Temussi PA

更新日期：1992-09-21 00:00:00

abstract：:We used Xenopus oocytes co-expressing thyrotropin-releasing hormone (TRH) receptors and human ether-a-go-go-related gene (HERG) K+ channel variants carrying different amino-terminal modifications to check the relevance of the proximal domain for hormonal regulation of the channel. Deletion of the whole proximal domain...

journal_title：FEBS letters

authors： Gómez-Varela D,Barros F,Viloria CG,Giráldez T,Manso DG,Dupuy SG,Miranda P,de la Peña P

更新日期：2003-01-30 00:00:00

abstract：:For more than three decades, RNA recombination remained a puzzle and has only begun to be solved in the last few years. The available data provide evidence for a variety of RNA recombination mechanisms. Non-homologous recombination seems to be the most common for RNA. Recent experiments in both the in vitro and the in...

journal_title：FEBS letters

authors： Chetverin AB

更新日期：1999-10-22 00:00:00

abstract：:A cDNA clone (pUP-23) corresponding to a member of a protein family that includes inhibitors of trypsin and of heterologous alpha-amylases has been selected from a library derived from developing barley endosperm and its sequence has been determined. A stretch of 95 nucleotides that included the signal peptide and the...

journal_title：FEBS letters

authors： Lázaro A,Rodriguez-Palenzuela P,Maraña C,Carbonero P,Garcia-Olmedo F

更新日期：1988-10-24 00:00:00

abstract：:A method was developed to study amino acids as possible precursors of PQQ biosynthesis. Cultures of Hyphomicrobium X, growing on [13C]methanol, were supplemented with unlabelled amino acids. Uptake and participation in metabolism were determined via gas chromatography/mass spectrometry of derivatized amino acids, obta...

journal_title：FEBS letters

authors： van Kleef MA,Duine JA

更新日期：1988-09-12 00:00:00

abstract：:Streptomyces lividans 1326-9, a bldA+ strain, and its bldA39 mutant derivative J1725 were transformed with a cosmid containing the pur cluster, which determines the puromycin biosynthetic pathway from Streptomyces alboniger. bldA+ transformants produced puromycin in typical amounts, whereas bldA39 transformants did so...

journal_title：FEBS letters

authors： Tercero JA,Espinosa JC,Jiménez A

更新日期：1998-01-16 00:00:00

abstract：:Abscisic acid (ABA) is a plant hormone involved in many developmental and physiological processes, but as yet, no ABA receptor has been identified. Flow cytometry of rice protoplasts and immunoblotting of purified plasma membranes (PMs) have been used to demonstrate that the monoclonal antibody JIM19 recognizes carboh...

journal_title：FEBS letters

authors： Desikan R,Hagenbeek D,Neill SJ,Rock CD

更新日期：1999-08-06 00:00:00

abstract：:In fibrous connective tissues, fibroblasts are organized into syncytia, cellular networks that enable matrix remodeling and that are interconnected by intercellular adherens junctions (AJs). The AJs of fibroblasts are mediated by N-cadherin, a broadly expressed classical cadherin that is critically involved in develop...

journal_title：FEBS letters

authors： El Sayegh TY,Kapus A,McCulloch CA

更新日期：2007-01-23 00:00:00

abstract：:Leaflets of Vicia faba with closed stomata or with opening stomata were freeze-dried. Excised guard-cell pairs were assayed individually under suboptimal conditions (pH 7.1 and subsaturating substrate) for phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) using quantitative histochemical procedures. L-Malate, 400 mi...

journal_title：FEBS letters

authors： Zhang SQ,Outlaw WH Jr,Chollet R

更新日期：1994-09-19 00:00:00

abstract：:WT1 was isolated as a tumor suppressor gene of Wilms tumor. However, high expression of WT1 correlates with poor prognosis in acute leukemia. In addition suppression of WT1 expression by WT1 anti-sense oligonucleotide inhibits proliferation of leukemia cells, suggesting that WT1 is important for their proliferation. T...

journal_title：FEBS letters

authors： Murata Y,Kudoh T,Sugiyama H,Toyoshima K,Akiyama T

更新日期：1997-06-02 00:00:00

abstract：:Antifungal activity has been associated with 2 immunochemically distinct proteins, protein R and S (Mr approximately 23 kDa; pI 9-10), which were isolated in pure form from barley grain. The proteins are homologous with thaumatin- and pathogenesis-related proteins of the PR5 family. The proteins inhibit growth of i.a....

journal_title：FEBS letters

authors： Hejgaard J,Jacobsen S,Svendsen I

更新日期：1991-10-07 00:00:00

abstract：:We quantitatively measured protective antigen (PA) binding to human cells targeted by anthrax lethal toxin (LT). Affinities were less than 50 nM for all cells, but differentiated cells (macrophages and neutrophils) had significantly increased PA binding and endothelial cells demonstrated the most binding. Combined wit...

journal_title：FEBS letters

authors： Deshpande A,Hammon RJ,Sanders CK,Graves SW

更新日期：2006-07-24 00:00:00

abstract：:The function of the coenzyme Q (CoQ) pool in the inner mitochondrial membrane is reviewed in view of recent findings suggesting a supramolecular organization of the mitochondrial respiratory complexes. In spite of the structural evidence for preferential aggregations of the inner membrane components, most kinetic evid...

journal_title：FEBS letters

authors： Lenaz G

更新日期：2001-12-07 00:00:00

abstract：:In the lysosomal storage disorder Gaucher disease (GD), glucosylceramide (GlcCer) accumulates due to the defective activity of glucocerebrosidase. A subset of GD patients develops neuropathology. We now show mislocalization of Limp2-positive puncta and a large reduction in the number of Lamp1-positive puncta, which ar...

journal_title：FEBS letters

authors： Zigdon H,Meshcheriakova A,Farfel-Becker T,Volpert G,Sabanay H,Futerman AH

更新日期：2017-03-01 00:00:00

abstract：:The human pathogen Vibrio vulnificus undergoes phase variation among colonial morphotypes, including a virulent opaque form which produces capsular polysaccharide (CPS) and a translucent phenotype that produces little or no CPS and is attenuated. Here, we found that a V. vulnificus mutant defective for RfaH antitermin...

journal_title：FEBS letters

authors： Garrett SB,Garrison-Schilling KL,Cooke JT,Pettis GS

更新日期：2016-12-01 00:00:00

abstract：:The informational content of the primary structure of thymopoietin (TP) is investigated using the informational spectrum method (ISM). We show that the sequence of TP shares common information with the sequences of long postsynaptic snake neurotoxins, although no apparent similarity was found among their primary struc...

journal_title：FEBS letters

authors： Skerl V,Pavlović M

更新日期：1988-10-24 00:00:00

abstract：:hTrp3 is a human homologue of the Drosophila gene responsible for a transient receptor potential (trp) mutation. When stably expressed in HEK293 cells, hTrp3 formed ion channels that were active under resting conditions but could be further stimulated by carbachol or ATP via endogenous muscarinic or purinergic recepto...

journal_title：FEBS letters

authors： Hurst RS,Zhu X,Boulay G,Birnbaumer L,Stefani E

更新日期：1998-02-06 00:00:00

abstract：:Single-pass sequences were obtained from the 5'-ends of a total of 1794 rose petal cDNA clones. Cluster analysis identified 242 groups of sequences and 635 singletons indicating that the database represents a total of 877 genes. Putative functions could be assigned to 1151 of the transcripts. Expression analysis indic...

journal_title：FEBS letters

authors： Channelière S,Rivière S,Scalliet G,Szecsi J,Jullien F,Dolle C,Vergne P,Dumas C,Bendahmane M,Hugueney P,Cock JM

更新日期：2002-03-27 00:00:00

abstract：:Cytosolic sialidase Neu2 has been implicated in myoblast differentiation. Here we observed a significant upregulation of Neu2 expression during differentiation of murine C2C12 myoblasts. This was evidenced both as an increase in Neu2 mRNA steady-state levels and in the cytosolic sialidase enzymatic activity. To unders...

journal_title：FEBS letters

authors： Fanzani A,Giuliani R,Colombo F,Zizioli D,Presta M,Preti A,Marchesini S

更新日期：2003-07-17 00:00:00

abstract：:OmpC, a surface antigen of Salmonella typhi was crystallized after several attempts, using PEG 3350. Well shaped hexagonal crystals were grown from vapor diffusion method using octyl glucoside and C12E9 as detergents. Crystals are sensitive to X-ray and diffract weakly up to 7 A. Porin isoforms, due to the bound lipop...

journal_title：FEBS letters

authors： Arockiasamy A,Krishnaswamy S

更新日期：1999-06-25 00:00:00

abstract：:Cytochrome c oxidase has been isolated from beef heart mitochondria and labeled with the fluorochrome eosin-5-maleimide (EMA) after pretreatment with mersalyl. On SDS-polyacrylamide gels, EMA fluorescence and absorption occurred at a single band corresponding to subunit III. Since only Cys 115 of the two cysteinyl res...

journal_title：FEBS letters

authors： Müller M,Azzi A

更新日期：1985-05-06 00:00:00

abstract：:The membrane-bound 3-chloro-4-hydroxyphenylacetate (Cl-OHPA) reductive dehalogenase from the chlorophenol-reducing anaerobe Desulfitobacterium hafniense was purified 11.3-fold to apparent homogeneity in the presence of the detergent CHAPS. The purified dehalogenase catalyzed the reductive dechlorination of Cl-OHPA to ...

journal_title：FEBS letters

authors： Christiansen N,Ahring BK,Wohlfarth G,Diekert G

更新日期：1998-10-02 00:00:00

abstract：:The TSH-induced cyclic AMP response was studied using a 3-year-old ovine thyroid cell line TSH-independent for growth: OVNIS 5H. The kinetics of cyclic AMP production was followed both in cell layers and in cell culture media, with or without phosphodiesterase inhibitor. It is noteworthy that following the first wave ...

journal_title：FEBS letters

authors： Fayet G,Aouani A,Hovsépian S

更新日期：1986-01-06 00:00:00