The puzzle of RNA recombination.

abstract：:Ku, a heterodimer of 70- and 80-kDa subunits, plays a general role in the metabolism of DNA ends in eukaryotic cells, including double-strand DNA break repair, V(D)J recombination, and maintenance of telomeres. We have utilized the yeast two-hybrid system to identify Ku70-interacting proteins other than Ku80. Two reac...

journal_title：FEBS letters

authors： Song K,Jung D,Jung Y,Lee SG,Lee I

更新日期：2000-09-08 00:00:00

abstract：:We investigated insulin's effect on intestinal lipid, transport and, particularly, the biogenesis of apolipoproteins crucial to lipoprotein secretion. Adding insulin (3 mU) to the serum-free medium of cultured jejunal explants from human fetuses (17-20 weeks) reduced triglyceride and chylomicron production and inhibit...

journal_title：FEBS letters

authors： Levy E,Sinnett D,Thibault L,Nguyen TD,Delvin E,Ménard D

更新日期：1996-09-16 00:00:00

abstract：:The collecting duct epithelium originates from the embryonic ureter by branching morphogenesis. Ontogeny-dependent changes of CFTR mRNA expression were assessed by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) in primary monolayer cultures of rat ureteric buds (UB) and cortical collecting ducts...

journal_title：FEBS letters

authors： Huber S,Braun G,Burger-Kentischer A,Reinhart B,Luckow B,Horster M

更新日期：1998-02-27 00:00:00

abstract：:The alpha3/beta4 subtype of neuronal nicotinic acetylcholine receptor (nAChR) was stably expressed in human embryonic kidney (HEK) 293 cells that co-expressed a voltage-gated Ca2+ channel. alpha3/beta4-nAChR-expressing clones were identified using the fura-2 Ca2+ imaging technique, and were further characterised by si...

journal_title：FEBS letters

authors： Stetzer E,Ebbinghaus U,Storch A,Poteur L,Schrattenholz A,Kramer G,Methfessel C,Maelicke A

更新日期：1996-11-11 00:00:00

abstract：:Prostaglandin E2 receptors (EPR), which belong to the family of heterotrimeric G protein-coupled ectoreceptors with seven transmembrane domains, can be classified into four subtypes according to their ligand binding and G protein coupling specificity. Of these, EP3betaR is coupled to Gi, whereas EP4R is coupled to Gs....

journal_title：FEBS letters

authors： Neuschäfer-Rube F,Hänecke K,Püschel GP

更新日期：1997-09-29 00:00:00

abstract：:In this work we have investigated the role of specific peptide sequences for glucose-inactivation of the yeast isocitrate lyase. Thus, different fragments of the ICL1 coding region were fused to the lacZ gene of E. coli to provide a reporter construction. Determinations of beta-galactosidase activities indicated that ...

journal_title：FEBS letters

authors： Ordiz I,Herrero P,Rodicio R,Moreno F

更新日期：1995-07-03 00:00:00

abstract：:Glucose-regulated protein 58 (GRP58)-like immunoreactivity in rat liver obtained in the evening or after fasting underwent an electrophoretic band-shift, which disappeared after phosphatase-treatment. Since mass spectrometric analysis raised a possibility that Ser150 of GRP58 is phosphorylated, an antibody against the...

journal_title：FEBS letters

authors： Kita K,Okumura N,Takao T,Watanabe M,Matsubara T,Nishimura O,Nagai K

更新日期：2006-01-09 00:00:00

abstract：:Regulatory T-cells (Tregs) are the guardians of peripheral tolerance acting to prevent autoimmune diseases such as systemic lupus erythomatosus (SLE) and rheumatoid arthritis (RA). Defects in Tregs have been reported in these two diseases despite significant differences in their clinical phenotype and pathogenesis. In...

journal_title：FEBS letters

authors： Chavele KM,Ehrenstein MR

更新日期：2011-12-01 00:00:00

abstract：:Several lines of evidence led to the conclusion that mammalian ribosomal protein synthesis is a highly organized biological process in vivo. A wealth of data support the concept according to which tRNA aminoacylation, formation of the ternary complex on EF1A and delivery of aminoacyl-tRNA to the ribosome is a processi...

journal_title：FEBS letters

authors： Mirande M

更新日期：2010-01-21 00:00:00

abstract：:A heat-stable activator for ADP-ribosylation factor (ARF)-dependent phospholipase D (PLD) was purified to near homogeneity from rat kidney cytosol by a sequential column chromatography. The purified activator has a molecular mass of 23 kDa on SDS-PAGE. Using a partially purified ARF-dependent PLD from rat kidney, the ...

journal_title：FEBS letters

authors： Akisue T,Jinnai H,Hitomi T,Miwa N,Yoshida K,Nakamura S

更新日期：1998-01-23 00:00:00

abstract：:Rabbits ingesting 4-fluorophenylalanine are known to incorporate small amounts of this fluorinated amino acid into their proteins. Carbonic anhydrase I isolated from the erythrocytes of animals so maintained exhibits a well-resolved fluorine NMR signal for each phenylalanine in the sequence. The chemical shifts of mos...

journal_title：FEBS letters

authors： Gamcsik MP,Gerig JT

更新日期：1986-02-03 00:00:00

abstract：:Fibromodulin and lumican are closely related members of the extracellular matrix leucine-rich repeat glycoprotein/proteoglycan family. Similar to decorin, another member of this protein family, they bind to fibrillar collagens and function in the assembly of the collagen network in connective tissues. We have studied ...

journal_title：FEBS letters

authors： Svensson L,Närlid I,Oldberg A

更新日期：2000-03-24 00:00:00

abstract：:Modelling the amino acid sequence of NAD(+)-linked 15-hydroxyprostaglandin dehydrogenase into the three-dimensional structure of 3 alpha/20 beta-hydroxysteroid dehydrogenase shows that these two enzymes, as well as the NADP(+)-linked prostaglandin dehydrogenase (identical to carbonyl reductase) have similar conformati...

journal_title：FEBS letters

authors： Krook M,Ghosh D,Duax W,Jörnvall H

更新日期：1993-05-10 00:00:00

abstract：:The tetracycline-controlled transcription system has become one of the most potent systems for experimental manipulations of transcription levels in vivo. Here we report on rtTA variants, which were generated by combining the existing positively regulated Tet repressor domains of rtTA and rtTA-M2 with a modified and m...

journal_title：FEBS letters

authors： Kämper MR,Gohla G,Schlüter G

更新日期：2002-04-24 00:00:00

abstract：:Pyruvate dehydrogenase (E1p) is one of the components of the pyruvate dehydrogenase multienzyme complex (PDHC). Previously, it was shown that the N-terminal domain of E1p is involved in its binding to the core component (E2p) of PDHC. We constructed point mutations in this domain (D17Q, D17R, E20Q, E20R, D24Q and D24R...

journal_title：FEBS letters

authors： Hengeveld AF,de Kok A

更新日期：2002-07-03 00:00:00

abstract：:We have investigated the possible role of selected negatively-charged amino acids of the sodium and chloride-coupled GABA transporter GAT-1 on sodium binding. These residues located adjacent to putative transmembrane domains and which are conserved throughout the large superfamily of neurotransmitter transporters were...

journal_title：FEBS letters

authors： Keshet GI,Bendahan A,Su H,Mager S,Lester HA,Kanner BI

更新日期：1995-08-28 00:00:00

abstract：:Polyubiquitylation leading to proteasomal degradation is a well-established mechanism for regulating TGF-β signal transduction components such as receptors and Smads. Recently, an equally important role was suggested for monoubiquitylation of both Smad4 and receptor-associated Smads that regulates their function witho...

journal_title：FEBS letters

authors： Dupont S,Inui M,Newfeld SJ

更新日期：2012-07-04 00:00:00

abstract：:We synthesized and evaluated by surface plasmon resonance 64 LNA/2'-O-methyl sequences corresponding to all possible combinations of such residues in a kissing aptamer loop complementary to the 6-nt loop of the TAR element of HIV-1. Three combinations of LNA/2'-O-methyl nucleoside analogues where one or two LNA units ...

journal_title：FEBS letters

authors： Di Primo C,Rudloff I,Reigadas S,Arzumanov AA,Gait MJ,Toulmé JJ

更新日期：2007-02-20 00:00:00

abstract：:Two polypeptides antigenically related to mammalian HMG1/2 have been detected in the yeast Saccharomyces cerevisiae. One exhibits an electrophoretic mobility in the range of mammalian HMG1/2 whereas the second polypeptide comigrates with yeast HMG S4. Evidence for the presence of HMG14/17 in yeast has not been obtaine...

journal_title：FEBS letters

authors： Estruch F,Pérez-Ortín JE,José M,Puigdomènech P,Franco L

更新日期：1986-03-03 00:00:00

abstract：:The trans----cis isomerization of Pro 93 was measured during refolding of bovine ribonuclease A. This isomerization is slow (tau = 500 s) under marginally stable folding conditions of 2.0 M GdmCl, pH 6, at 10 degrees C. However, it is strongly accelerated (tau = 100 s) in samples which, prior to isomerization, had bee...

journal_title：FEBS letters

authors： Schmid FX

更新日期：1986-03-31 00:00:00

abstract：:We have shown by electrophoretic mobility shift assays that the nucleotide sequence CE-LPH1, centred at position -49 with respect to the transcription start site of the rat gene encoding intestinal lactase-phlorizin hydrolase, interacts in vitro with nuclear proteins present in the jejunum of suckling animals. Protein...

journal_title：FEBS letters

authors： Boukamel R,Freund JN

更新日期：1994-10-10 00:00:00

abstract：:Constitutively secreted proteins have traditionally been believed to be excluded from the regulated secretory pathway. In this work we show that kappa light chain and Fc fragment, two markers of the constitutive pathway, are present in the regulated pathway in AtT-20 cells. They colocalize with the endogenous hormone ...

journal_title：FEBS letters

authors： Castle AM,Huang AY,Castle JD

更新日期：1998-11-20 00:00:00

abstract：:To understand the role of nitric oxide (NO) in controlling the specific DNA-binding activities of transcriptional factors, we investigated the in vitro effect of the NO-donor sodium nitroprusside (SNP) on the AP-1 activity of cultured mouse cerebellar granule cells. A gel-mobility assay showed that SNP inhibited AP-1 ...

journal_title：FEBS letters

authors： Tabuchi A,Sano K,Oh E,Tsuchiya T,Tsuda M

更新日期：1994-08-29 00:00:00

abstract：:Acceptor stem variants of tRNA(fMet) (Escherichia coli) have been characterized by nuclear magnetic resonance. The wild type contains a C1-A72 mismatch pair which is crucial for its biological function. For comparison, the mismatch was replaced by regular pairs U1-A72 and C1-G72. Further variants contain an altered di...

journal_title：FEBS letters

authors： Zuleeg T,Vogtherr M,Schübel H,Limmer S

更新日期：2000-04-28 00:00:00

abstract：:The Rad51 gene of Saccharomyces cerevisiae is required for genetic recombination and recombinational repair of DNA strand breaks. In higher eukaryotes Rad51 is essential for embryonic development, and is involved in cell proliferation and DNA repair. Here we show that human Rad51 (HsRad51) is proteolytically cleaved d...

journal_title：FEBS letters

authors： Flygare J,Armstrong RC,Wennborg A,Orsan S,Hellgren D

更新日期：1998-05-08 00:00:00

abstract：:The RhoGTPase Rac1 is activated in a polarised fashion and controls cell motility. We previously showed that Rac1 binds the PP2A inhibitor SET and recruits nuclear SET to the cytosol. We show that a SET mutant, lacking a nuclear localization signal, SET(ΔNLS), promotes cell spreading and motility. This was accompanied...

journal_title：FEBS letters

authors： Lam BD,Anthony EC,Hordijk PL

更新日期：2013-01-16 00:00:00

abstract：:The nuclear factor NF-IL6 had been suggested to be responsible for the IL-6-mediated induction of several acute-phase proteins. To obtain evidence for the involvement of NF-IL6 in the induction of acute-phase proteins, we introduced the NF-IL6 gene and its truncated mutant (delNFIL6) gene into a hepatoma cell line Hep...

journal_title：FEBS letters

authors： Natsuka S,Isshiki H,Akira S,Kishimoto T

更新日期：1991-10-07 00:00:00

abstract：:In this work, we examined the antioxidant role of mucin, a typical sialic acid containing high-molecular weight glycoprotein. The function of mucin as a hydroxyl radical (.OH) scavenger was characterized using bovine submaxillary gland mucin (BSM). Non-treated BSM effectively protected DNA from the attack of .OH; howe...

journal_title：FEBS letters

authors： Ogasawara Y,Namai T,Yoshino F,Lee MC,Ishii K

更新日期：2007-05-29 00:00:00

abstract：:The human ether-a-go-go-related gene (herg) encodes a K+ current (I(HERG)) which plays a fundamental role in heart excitability and in neurons by contributing to action potential repolarization and to spike-frequency adaptation, respectively. In this paper we show that I(HERG), recorded in neuroblastoma cells and guin...

journal_title：FEBS letters

authors： Rosati B,Rocchetti M,Zaza A,Wanke E

更新日期：1998-11-27 00:00:00

abstract：:The mitochondrial ADP/ATP carriers (AACs) catalyze the exchange of cytosolic ADP for matrix ATP. We have identified and characterized a novel member of the AAC subfamily of mitochondrial metabolite transport proteins, termed AAC4. The AAC4 gene maps to human chromosome 4q28.1, and its product AAC4 is 66-68% identical ...

journal_title：FEBS letters

authors： Dolce V,Scarcia P,Iacopetta D,Palmieri F

更新日期：2005-01-31 00:00:00