A fourth ADP/ATP carrier isoform in man: identification, bacterial expression, functional characterization and tissue distribution.

abstract：:In cultured rat pituitary gonadotrophs, gonadotropin-releasing hormone (GnRH) induces rapid hyperpolarization of the cell membrane and causes cessation of the spontaneous electrical activity present in non-stimulated cells. This initial response to GnRH is followed by slow oscillations of membrane potential (Vm) which...

journal_title：FEBS letters

authors： Kukuljan M,Stojilković SS,Rojas E,Catt KJ

更新日期：1992-04-13 00:00:00

abstract：:A 4.8 kb DNA-fragment was cloned and sequenced encompassing the structural gene of PFL-deactivase (2.7 kb) and 2 kb of the 5' flanking region that contains the elements for anaerobic induction. A mutant lacking deactivase was shown to require exogenous electron acceptors for anaerobic growth with glucose. This reveale...

journal_title：FEBS letters

authors： Kessler D,Leibrecht I,Knappe J

更新日期：1991-04-09 00:00:00

abstract：:A potent inhibitor of a dual-specificity protein phosphatase, VHR (vaccinia H1 related), was isolated during a screening of microbial metabolites. This inhibitor was identified as 4-isoavenaciolide (4-iA), and was determined to irreversibly inhibit VHR phosphatase activity with a 50% inhibitory concentration of 1.2 mi...

journal_title：FEBS letters

authors： Ueda K,Usui T,Nakayama H,Ueki M,Takio K,Ubukata M,Osada H

更新日期：2002-08-14 00:00:00

abstract：:In spite of numerous studies, there appears to be no consensus regarding the orientation and aggregation state of membrane-bound melittin. We report here the restricted environment of the sole tryptophan residue in membrane-bound melittin using environment-induced effects on the rates of solvent relaxation. When incor...

journal_title：FEBS letters

authors： Chattopadhyay A,Rukmini R

更新日期：1993-12-13 00:00:00

abstract：:Transcriptional co-activators, co-repressors and chromatin remodeling machines are essential elements in the transcriptional programs directed by the master adipogenic transcription factor PPARgamma. Many of these components have orthologs in other organisms, where they play roles in development and pattern formation,...

journal_title：FEBS letters

authors： Denis GV,Nikolajczyk BS,Schnitzler GR

更新日期：2010-08-04 00:00:00

abstract：:The carboxy-terminal half of the c-src protein fused to the protein A moiety was expressed in bacteria. The protein A/truncated c-src fusion protein, which does not have SH2 and SH3 domains, is found in the periplasmic space allowing for a simple one-step purification and demonstrated high efficiency in autophosphoryl...

journal_title：FEBS letters

authors： Saya H,Lee PS,Nishi T,Izawa I,Nakajima M,Gallick GE,Levin VA

更新日期：1993-07-26 00:00:00

abstract：:The thermal stability of PQQ glucose dehydrogenases (PQQGDHs) which were chimeras with more than 95% made up of the N-terminal region of Escherichia coli PQQGDH and the rest made up of the C-terminal region of Acinetobacter calcoaceticus PQQGDH was investigated. Among the chimeric PQQGDHs, E97A3 (E. coli 97% and A. ca...

journal_title：FEBS letters

authors： Sode K,Watanabe K,Ito S,Matsumura K,Kikuchi T

更新日期：1995-05-15 00:00:00

abstract：:RNA binding motif protein 5 (RBM5) is a candidate tumor suppressor gene. Recent studies showed that RBM5 functions as an alternative splicing regulator of apoptosis-related genes. Here, we identify DHX15 and PRP19, two spliceosome components, as novel RBM5-interacting partners. We then show that the G-patch domain of ...

journal_title：FEBS letters

authors： Niu Z,Jin W,Zhang L,Li X

更新日期：2012-04-05 00:00:00

abstract：:We have previously reported that Monad, a novel WD40 repeat protein, potentiates apoptosis induced by tumor necrosis factor-alpha(TNF-alpha) and cycloheximide (CHX). By affinity purification and mass spectrometry, we identified RNA polymerase II-associated protein 3 (RPAP3) as a binding protein of Monad. Overexpressio...

journal_title：FEBS letters

authors： Itsuki Y,Saeki M,Nakahara H,Egusa H,Irie Y,Terao Y,Kawabata S,Yatani H,Kamisaki Y

更新日期：2008-07-09 00:00:00

abstract：:Mammalian heart has been reported to express AC isozymes (types V and VI) that are inhibited by < microM [Ca2+]; avian heart has been reported to express adenylyl cyclase activity that is inhibited by < microM [Ca2]. We have used reverse transcription polymerase chain reaction (RT-PCR) to determine that type V and VI ...

journal_title：FEBS letters

authors： Yu HJ,Unnerstall JR,Green RD

更新日期：1995-10-23 00:00:00

abstract：:Analysis of glutamic acid decarboxylase (GDC) (EC 4.1.1.15) from Escherichia coli ATCC 11246 revealed the presence of six pyridoxal phosphates (PLPs) as well as six covalently bound pyrroloquinoline quinones (PQQs) per hexameric enzyme molecule. This is the second example of a pyridoxo-quinoprotein, suggesting that ot...

journal_title：FEBS letters

authors： van der Meer RA,Groen BW,Duine JA

更新日期：1989-03-27 00:00:00

abstract：:The structure of the Ala38 variant of yeast iso-1-cytochrome c, in which the previously unchanged Arg38 has been replaced, has been characterised by NMR. The NMR data indicate that the structure of the Ala38 variant is very similar to that of the wild type protein. In particular, the heme environment and interactions ...

journal_title：FEBS letters

authors： Thurgood AG,Pielak GJ,Cutler RL,Davies AM,Greenwood C,Mauk AG,Smith M,Williamson DJ,Moore GR

更新日期：1991-06-24 00:00:00

abstract：:Neprilysin-2 (NEP2) is a novel metallopeptidase homologous to neprilysin (NEP), an enzyme involved in regulation of neuropeptide signalling. NEP2 exists as two alternatively spliced isoforms, NEP2 and NEP2(Delta). In this study, we cloned and expressed both human isoforms. Human NEP2 exists as a membrane-bound and sol...

journal_title：FEBS letters

authors： Whyteside AR,Turner AJ

更新日期：2008-07-09 00:00:00

abstract：:Aah VI was isolated from the venom of the North African scorpion, Androctonus australis hector. It is the first glycosylated neurotoxin from scorpion venom to be described. It was not toxic to mice, when injected intracerebroventricularly at a dose of 1.2 microg per animal. However, it had typical activity in Blatella...

journal_title：FEBS letters

authors： Hassani O,Loew D,Van Dorsselaer A,Papandréou MJ,Sorokine O,Rochat H,Sampieri F,Mansuelle P

更新日期：1999-01-25 00:00:00

abstract：:A variety of mutations leading to amino acid substitutions have been described in the transthyretin gene in association with different familial amyloidoses and have been implicated to be involved in the pathogenesis of amyloid deposits. However, there has been disagreement whether or not a transthyretin mutation is pr...

journal_title：FEBS letters

authors： Christmanson L,Betsholtz C,Gustavsson A,Johansson B,Sletten K,Westermark P

更新日期：1991-04-09 00:00:00

abstract：:Elongation factor EF-Tu from Escherichia coli was labelled with N-[14C]tosyl-L-phenylalanylchloromethane, digested with trypsin and the peptides obtained separated by HPLC. The only radioactive peak recovered corresponded to tryptic peptide containing residues 75-98. Sequencing of the peptide by automated Edman degrad...

journal_title：FEBS letters

authors： Jonák J,Petersen TE,Clark BF,Rychlík I

更新日期：1982-12-27 00:00:00

abstract：:Many receptors coupled to inhibitory Go/Gi-type G proteins often also produce stimulatory signals like Ca2+ mobilisation. When expressed in CHO cells the alpha2-adrenoceptor subtypes alpha2A, alpha2B and alpkha2C mobilised Ca2+. These responses were strongly reduced by the P2Y-purinoceptor antagonist suramin. A large ...

journal_title：FEBS letters

authors： Akerman KE,Näsman J,Lund PE,Shariatmadari R,Kukkonen JP

更新日期：1998-07-03 00:00:00

abstract：:The Kv3.1 channel plays a crucial role in regulating the high-frequency firing properties of neurons. Here, we determined whether Src regulates the subcellular distributions of the Kv3.1b channel. Co-expression of active Src induced a dramatic redistribution of Kv3.1b to the endoplasmic reticulum. Furthermore, co-expr...

journal_title：FEBS letters

authors： Bae SH,Kim DH,Shin SK,Choi JS,Park KS

更新日期：2014-01-03 00:00:00

abstract：:cDNAs encoding aquaporins PIP1;1, PIP2;1, and TIP1;1 were isolated from Mimosa pudica (Mp) cDNA library. MpPIP1;1 exhibited no water channel activity; however, it facilitated the water channel activity of MpPIP2;1 in a phosphorylation-dependent manner. Mutagenesis analysis revealed that Ser-131 of MpPIP1;1 was phospho...

journal_title：FEBS letters

authors： Temmei Y,Uchida S,Hoshino D,Kanzawa N,Kuwahara M,Sasaki S,Tsuchiya T

更新日期：2005-08-15 00:00:00

abstract：:The Methanocaldococcus jannaschii (formerly Methanococcus jannaschii) protein Mj0968 has been reported to represent a soluble P-type ATPase [Ogawa et al., FEBS Lett. 471 (2000) 99-102]. In this study, we report that the heterologously expressed Mj0968-His(10) protein exhibits high rates of phosphatase activity, wherea...

journal_title：FEBS letters

authors： Bramkamp M,Gassel M,Herkenhoff-Hesselmann B,Bertrand J,Altendorf K

更新日期：2003-05-22 00:00:00

abstract：:Fibroblast growth factor-2 (FGF-2) is mitogenic for the human breast cancer cell line MCF-7; here we investigate some of the signaling pathways subserving this activity. FGF-2 stimulation of MCF-7 cells resulted in a global increase of intracellular tyrosine phosphorylation of proteins, particularly FGF receptor subst...

journal_title：FEBS letters

authors： Vercoutter-Edouart A,Lemoine J,Smart CE,Nurcombe V,Boilly B,Peyrat J,Hondermarck H

更新日期：2000-08-04 00:00:00

abstract：:alphaArg-376, betaLys-155, and betaArg-182 are catalytically important ATP synthase residues that were proposed to be involved in substrate Pi binding and subsequent steps of ATP synthesis [Senior, A.E., Nadanaciva, S. and Weber, J. (2002) Biochim. Biophys. Acta 1553, 188-211]. Here, it was shown using purified Escher...

journal_title：FEBS letters

authors： Ahmad Z,Senior AE

更新日期：2005-01-17 00:00:00

abstract：:Hic-5/ARA55 is a LIM-only member of the paxillin superfamily. Conflicting reports have suggested that Hic-5/ARA55 can both repress and enhance a number of biological processes, including myogenesis and tumorigenesis. With two Hic-5 isoforms documented, we hypothesized that multiple Hic-5 isoforms may exist that have b...

journal_title：FEBS letters

authors： Gao Z,Schwartz LM

更新日期：2005-10-24 00:00:00

abstract：:Rho proteins and phosphatidylinositide 3-kinases (PI 3-kinases) have been widely implicated in regulating cell motility both in cultured cells and in animal models. Monocytes are recruited from the bloodstream in response to inflammatory signals, and migrate across the endothelial barrier into the tissues, where they ...

journal_title：FEBS letters

authors： Ridley AJ

更新日期：2001-06-08 00:00:00

abstract：:Drosophila PTEN (dPTEN) plays indispensable roles in the development of Drosophila melanogaster by controlling cell size and number. Although three potential spliced forms of dPTEN have been isolated, functional distinction among these forms remains elusive. In this study, we demonstrate that all spliced forms of dPTE...

journal_title：FEBS letters

authors： Maehama T,Kosaka N,Okahara F,Takeuchi K,Umeda M,Dixon JE,Kanaho Y

更新日期：2004-05-07 00:00:00

abstract：:Using ultrastructural analysis and labeling with polyclonal antibodies that recognize peptide sequences specific for phospholipid binding, we mapped the functional domain structure of intact platelet talin and its proteolytic fragments. The talin dimer, which is crucial for actin and lipid binding, is built of a backb...

journal_title：FEBS letters

authors： Isenberg G,Goldmann WH

更新日期：1998-04-17 00:00:00

abstract：:Saccharomyces cerevisiae Vig4/Vrg4 protein is a Golgi membrane protein which has multiple transmembrane domains and is essential for transport of GDP-mannose across the Golgi membrane. By immunoprecipitation of detergent-solubilized tagged protein, we found that this protein exists as oligomer. Two mutants vig4-1 and ...

journal_title：FEBS letters

authors： Abe M,Hashimoto H,Yoda K

更新日期：1999-09-24 00:00:00

abstract：:We have isolated a potent platelet aggregation inducer from the crude venom of Calloselasma rhodostoma (Malayan pit viper), termed rhodoaggretin, with a novel oligomeric structure consisting of a dimer of C-type lectin-like heterodimers. On the basis of its native molecular mass of 66 kDa, and a M(r) of 30 kDa for its...

journal_title：FEBS letters

authors： Wang R,Kong C,Kolatkar P,Chung MC

更新日期：2001-11-23 00:00:00

abstract：:Histone acetyltransferase activity was measured in isolated nuclei during the synchronous cell cycle of the myxomycete Physarum polycephalum. Nuclei were incubated with [14C]acetyl-coenzyme A and an excess of exogenous calf thymus histones. The activity is periodic during the cell cycle; it rises during the S-phase to...

journal_title：FEBS letters

authors： Golderer G,Loidl P,Gröbner P

更新日期：1987-10-05 00:00:00

abstract：:Promoter activity of protein kinase C (PKC) gamma gene was analysed by chloramphenicol acetyltransferase (CAT) assay using extracts from the cells transfected with various fusion constructs containing the 5'-flanking region of the mouse PKC gamma gene and CAT gene. Transient expression experiments in PC12 cells reveal...

journal_title：FEBS letters

authors： Takanaga H,Mukai H,Shimakawa M,Konishi H,Kikkawa U,Koizumi T,Ono Y

更新日期：1995-07-17 00:00:00