Abstract:
:A 4.8 kb DNA-fragment was cloned and sequenced encompassing the structural gene of PFL-deactivase (2.7 kb) and 2 kb of the 5' flanking region that contains the elements for anaerobic induction. A mutant lacking deactivase was shown to require exogenous electron acceptors for anaerobic growth with glucose. This revealed the identity of PFL-deactivase with the alcohol and acetaldehyde dehydrogenases of E. coli. The multienzyme represents a homopolymeric protein (approximately 40 x 96 kDa) requiring Fe2+ for all functions.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kessler D,Leibrecht I,Knappe Jdoi
10.1016/0014-5793(91)80358-asubject
Has Abstractpub_date
1991-04-09 00:00:00pages
59-63issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(91)80358-Ajournal_volume
281pub_type
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