Abstract:
:Plastoquinone-9 (PQ-9)-depleted PSII reaction center core complex, consisting of CP47/D1/D2/Cytb-559/I, was isolated from spinach PSII particles. PQ-9, lipids and several proteins were extracted from the original PSII particles and separated by several steps of chromatography to be reconstituted into the isolated complex. PQ-9 reconstituted in the complex with the help of thylakoid lipids (digalactosyldiglyceride) did not function as QA by itself. However, PQ-9 simultaneously reconstituted with L protein and the thylakoid lipids successfully functioned as QA in the complex. Other proteins of PSII origin, such as CP43, H, K, nuclear encoded 4.1 and 5.0 kDa proteins, are unable to restore the QA activity in the complex.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kitamura K,Ozawa S,Shiina T,Toyoshima Ydoi
10.1016/0014-5793(94)01089-7subject
Has Abstractpub_date
1994-10-31 00:00:00pages
113-6issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(94)01089-7journal_volume
354pub_type
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