Abstract:
:In Xanthomonas campestris, the causative agent of black rot in crucifers, the endoglucanase level is greatly decreased in the mutant deficient in Clp, a homologue of cyclic AMP receptor protein (CRP). It is established that Clp has the same DNA binding specificity as CRP at positions 5, 6, and 7 (GTG motif) of the DNA half site. In this study, the engA transcription initiation site was determined by the 5' RACE method, and two consensus Clp-binding sites, site I and site II centered at -69.5 and -42.5, respectively, were located. Transcriptional fusion assays indicated that Clp greatly activates engA transcription. Site-directed mutagenesis indicated that position 5 of GTG motif in site II is essential for both DNA-protein complex formation in electrophoretic mobility shift assays and engA transcription in vivo. In addition, mutation at position 5 of site I drastically reduces the promoter activity, indicating that binding of Clp to site I exerts a synergistic effect on the transcription activation by site II. engA appears to be the first X. campestris gene known to be activated by Clp via a direct binding to the promoter.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Hsiao YM,Liao HY,Lee MC,Yang TC,Tseng YHdoi
10.1016/j.febslet.2005.05.023keywords:
subject
Has Abstractpub_date
2005-07-04 00:00:00pages
3525-33issue
17eissn
0014-5793issn
1873-3468pii
S0014-5793(05)00630-7journal_volume
579pub_type
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